Reinforcement Fibrin-Hyaluronic Acid Composite Gel for Tissue Engineering Cartilage Genesis

2007 ◽  
Vol 342-343 ◽  
pp. 153-156 ◽  
Author(s):  
Sang Hyug Park ◽  
So Ra Park ◽  
Byoung Hyun Min
Keyword(s):  
Hyaluronic Acid ◽  
Structural Integrity ◽  
Cell Delivery ◽  
Fibrin Gel ◽  
External Compression ◽  
Composite Gels ◽  
Composite Gel ◽  
A Cell ◽  
Safranin O ◽  
Hybrid Biomaterials

The reimplantation method of cultured chondrocytes broadly has been offered as an alternative for articular cartilage repair. A variety of biologically derived and synthetic polymeric and hydrogel materials also have been investigated for good cell delivery efficiency. Preciously, we examined the feasibility of fibrin gel, mixed with hyaluronic acid(HA) as a cell delivery carrier. In order to reinforce the material, hybrid biomaterials of fibrin/HA composite gels with fibrinolysis inhibition factors(FIFs: aprotinin, DI101, EACA) have been investigated in the present work because we did not satisfy a little progress. These fibrin/HA composite gels added FIFs maintained their structural integrity in long-term culture over 4th weeks. Contrary to our expectation the mass of the fibrin/HA composite with DI 101 was significantly superior to the ones of other combinations. In histological evidence, all of them are showed good positive result of stain of Safranin-O and alcian blue during the culture period. In gross examination, samples of all groups grossly resembled cartilage in color and were resistant to external compression. Our study demonstrates that most favorable polymer can be used good quality tissue engineered cartilage and in this culture systems have been useful for studying the basic biology of chondrocyte biosynthesis of ECM and new cartilage matrix formation without a loss of volume. After all, we proved the safety of inhibitors of the fibrinolytic system without hazardous effect on cell behavior and found out that DI 101 would be the most effective agent.

Potential of Fortified Fibrin/Hyaluronic Acid Composite Gel as a Cell Delivery Vehicle for Chondrocytes

2009 ◽  
Vol 33 (6) ◽  
pp. 439-447 ◽  
Author(s):  
Sang-Hyug Park ◽  
Ji Hao Cui ◽  
So Ra Park ◽  
Byoung-Hyun Min
Keyword(s):  
Hyaluronic Acid ◽  
Cell Delivery ◽  
Delivery Vehicle ◽  
Composite Gel ◽  
A Cell

Fabrication of Chemically Linked PEGylated Prodrugs for Hydrogel Carriers

2021 ◽  
Vol 105 ◽  
pp. 42-47
Author(s):  
Tian Le Wang
Keyword(s):  
Hyaluronic Acid ◽  
Drug Loading ◽  
Reaction Synthesis ◽  
Alendronate Sodium ◽  
Hydrophilic Drugs ◽  
Chemical Structures ◽  
Composite Gels ◽  
Composite Gel ◽  
Different Types ◽  
Osteoporosis Drug

Some hydrophilic drugs (e.g. anti-tumor drug of doxorubicin (DOX) and anti-osteoporosis drug of alendronate sodium (ALN)) have great toxic and adverse effects on the human body. In this paper, two kinds of drug-loaded composite gel systems were prepared via mild and efficient chemical reaction synthesis, hyaluronic acid-linked ALN (HA-ALN) hydrogel and polyethylene glycol-linked DOX (Tetra-PEG-DOX) hydrogel. By characterizing their chemical structures, morphological networks, and ultraviolet absorption behaviors, two different types of drug-loaded composite gels can be constructed well. It is expected to achieve effective drug loading and controlled release. The two drug-loaded gels are applied in the fields of antitumor and anti-osteoporosis and exert their application value in the field of biomedicine.

scholarly journals Echocardiography-guided percutaneous left ventricular intracavitary injection as a cell delivery approach in infarcted mice

2021 ◽  
Vol 476 (5) ◽  
pp. 2135-2148
Author(s):  
Yibing Nong ◽  
Yiru Guo ◽  
Alex Tomlin ◽  
Xiaoping Zhu ◽  
Marcin Wysoczynski ◽  
...  
Keyword(s):  
Left Ventricular ◽  
Cell Delivery ◽  
A Cell ◽  
Delivery Approach

Development of a cell delivery system using alginate microbeads for tissue regeneration

2016 ◽  
Vol 4 (20) ◽  
pp. 3515-3525 ◽  
Author(s):  
Shirae K. Leslie ◽  
Anthony M. Nicolini ◽  
Gobalakrishnan Sundaresan ◽  
Jamal Zweit ◽  
Barbara D. Boyan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Delivery System ◽  
Tissue Regeneration ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Viable Cells ◽  
A Cell

Alginate microbeads incorporating adipose-derived stem cells (ASCs) have potential for delivering viable cells capable of facilitating tissue regeneration.

scholarly journals In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yuqing Niu ◽  
Massimiliano Galluzzi ◽  
Ming Fu ◽  
Jinhua Hu ◽  
Huimin Xia
Keyword(s):  
Hyaluronic Acid ◽  
Carotid Artery ◽  
Structural Integrity ◽  
Composite Scaffold ◽  
Vascular Endothelial Cells ◽  
Rabbit Model ◽  
Vascular Reconstruction ◽  
Vascular Prostheses ◽  
Nanofibrous Scaffolds

AbstractOne of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia. The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model. Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method. The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood. Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency. At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery. This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction. Graphical Abstract

scholarly journals Tissue adhesive hyaluronic acid hydrogels for sutureless stem cell delivery and regeneration of corneal epithelium and stroma

Biomaterials ◽  
2019 ◽  
Vol 225 ◽  
pp. 119516 ◽  
Author(s):  
Laura Koivusalo ◽  
Maija Kauppila ◽  
Sumanta Samanta ◽  
Vijay Singh Parihar ◽  
Tanja Ilmarinen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Corneal Epithelium ◽  
Tissue Adhesive ◽  
Cell Delivery ◽  
Stem Cell Delivery

A novel fibrin gel derived from hyaluronic acid-grafted fibrinogen

2011 ◽  
Vol 6 (2) ◽  
pp. 025009 ◽  
Author(s):  
Chiung L Yang ◽  
Hui W Chen ◽  
Tzu C Wang ◽  
Yng J Wang
Keyword(s):  
Hyaluronic Acid ◽  
Fibrin Gel

Collagen Network Architecture Varies Between Pure Collagen and Collagen-Fibrin Co-Gels

2012 ◽  
Author(s):  
Victor K. Lai ◽  
Allan M. Kerandi ◽  
Spencer P. Lake ◽  
Robert T. Tranquillo ◽  
Victor H. Barocas
Keyword(s):  
Mechanical Behavior ◽  
Network Architecture ◽  
Rational Design ◽  
Structural Integrity ◽  
Collagen Network ◽  
Fibrin Gel ◽  
Ecm Proteins ◽  
Fibrin Networks ◽  
Pure Collagen ◽  
Function Relationship

Naturally-occurring extracellular matrix (ECM) proteins, e.g. collagen I and fibrin, play an important role in tissues, conferring structural integrity and providing a biochemical environment for eliciting important cellular responses (e.g. migration). Tissue engineers use a variety of matrix polymers as initial scaffolds for seeding cells, sometimes in combination with one another (e.g. collagen-fibrin [1]). For example, our group fabricates arterial tissue equivalents (TEs) by seeding cells in a fibrin gel, which is gradually degraded over time and replaced by cell-produced collagen [2]. While the structure and mechanics of individual ECM proteins have been studied extensively, how multiple fibrillar networks interact to confer overall mechanical behavior remains poorly understood. Narrowing this gap in knowledge of scaffolds comprising multiple fibril networks is crucial in allowing for more rational design in tissue engineering, as cells react differently according to their mechanical environments. For collagen-fibrin networks in particular, early efforts in elucidating interactions between these two fibril networks in co-gels have proven inconclusive due to inconsistent findings from various groups. Recent modeling efforts by our group have shown that simple “series” and “parallel” type interactions provide bounds for the mechanical behavior of collagen-fibrin co-gels [3]. In addition, experiments on pure collagen and fibrin vs. their respective networks from collagen-fibrin co-gels after digestion showed slight differences in mechanical behavior [4]. These previous studies have focused on the composition-function relationship between collagen and fibrin. The objective of the current work is to explore how collagen network architecture changes in the presence of the fibrin network in collagen-fibrin co-gels, thereby providing an added dimension to our understanding of collagen-fibrin systems by elucidating structure-composition-function relationships between collagen and fibrin.

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