PEGylated Plier-Like Cationic Niosomes on Gene Delivery in HeLa Cells

2019 ◽  
Vol 819 ◽  
pp. 151-156
Author(s):  
Supusson Pengnam ◽  
Samarwadee Plianwong ◽  
Kanokwan Singpanna ◽  
Nattisa Ni-yomtham ◽  
Widchaya Radchatawedchakoon ◽  
...  
Keyword(s):  
Particle Size ◽  
Gene Delivery ◽  
Hela Cells ◽  
Transfection Efficiency ◽  
Cationic Lipid ◽  
Dna Delivery ◽  
Molar Ratio ◽  
The Novel ◽  
Low Cytotoxicity ◽  
Cationic Niosomes

Lipid-based formulations have been used as a widespread carrier to improve gene delivery. Niosomes, one type of lipid-based vesicular systems are produced from non-ionic surfactants which are generally inexpensive and potentially more stable than phospholipids. This article was to develop PEGylated cationic niosomes for DNA delivery. Thin film hydration and sonication method were applied for cationic niosomes. The niosome formulations were composed of Span 20, cholesterol (Chol) and plier-like cationic lipid B (PCL-B) with or without cholesterol-polyethylene glycol 2000 (Chol-PEG). The physicochemical properties of cationic niosomes and nioplexes were evaluated including particle size, zeta potential, DNA condensation and serum protection. The transfection efficiency and cell viability were examined in HeLa cells. The particle size and surface charge of PEGylated cationic niosome containing Span 20: Chol: PCL-B: Chol-PEG at the molar ratio of 2.5: 2.5: 1.5: 0.14 (N-PEG2) were 129.47 ± 2.15 nm and 25.93 ± 4.18 mV, respectively. These PEGylated cationic niosomes could condense pDNA into the nanosize particles and also enhance the serum protection ability for at least 6 h. Moreover, N-PEG2 exhibited high transfection efficiency in comparison with lipofectamine® 2000 and low cytotoxicity. Therefore, the novel PEGylated cationic niosomes have the capability to develop as a promising potential carrier for DNA delivery.

scholarly journals Influence of serum on DNA protection ability and transfection efficiency of cationic lipid-based nanoparticles for gene delivery

2018 ◽  
Vol 192 ◽  
pp. 01025 ◽  
Author(s):  
Supusson Pengnam ◽  
Lalita Leksantikul ◽  
Prasopchai Tonglairoum ◽  
Praneet Opanasopit ◽  
Nattisa Ni-yomtham ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Cationic Lipid ◽  
Dna Degradation ◽  
Delivery Systems ◽  
Agarose Gel Electrophoresis ◽  
Dna Protection ◽  
Significant Difference ◽  
Nanoparticulate Systems ◽  
Cationic Niosomes

Cationic lipid-based nanoparticulate systems are delivery systems that has been widely used in pharmaceutical field including gene delivery. There are many barriers obstructing genetic materials and their delivery systems to reach the target. Serum is one of the imperative factor that should be investigated. Therefore, the aim of this study was to examine the effect of serum on DNA protection ability of spermine-liposomes and niosomes by evaluating the percentage of transfection efficiency in Hela cell and observing the DNA degradation band using agarose gel electrophoresis in the presence of serum. The results showed that the percentage of transfection efficiency of spermine-liposomes was dramatically decreased when serum is presented (p< 0.05). In contrast, whether or not the serum is presented, the spermine-niosomes showed no significant difference in transfection efficiency. Concisely, liposomes could slightly protect DNA from DNase in the serum, whereas, niosomes had potential ability to protect DNA from the enzymes in serum. This result revealed an advantage of the cationic niosomes system as a gene carrier over the cationic liposomes.

Design and synthesis of a fluorescent amino poly(glycidyl methacrylate) for efficient gene delivery

2019 ◽  
Vol 7 (11) ◽  
pp. 1875-1881 ◽  
Author(s):  
Hongtao Chen ◽  
Shusen You ◽  
Qing Cai ◽  
Yang Zheng ◽  
Liqun Zhang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Glycidyl Methacrylate ◽  
Dna Delivery ◽  
Atom Transfer ◽  
Design And Synthesis ◽  
Efficient Gene ◽  
Low Cytotoxicity ◽  
Delivery Efficiency

A fluorescent amino poly(glycidyl methacrylate) (PGOHMA) was synthesized by atom transfer radical polymerization (ATRP) and post-polymerization. PGOHMA has low cytotoxicity and high DNA delivery efficiency.

Cholic Acid-Modified Polyethylenimine for Gene Delivery into Human Carcinoma Cells

2014 ◽  
Vol 1060 ◽  
pp. 3-6 ◽  
Author(s):  
Wanlop Weecharangsan ◽  
Orapan Paecharoenchai ◽  
Nattisa Niyomtham ◽  
Praneet Opanasopit ◽  
Boon-ek Yingyongnarongkul ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Zeta Potential ◽  
Cholic Acid ◽  
Plasmid Dna ◽  
Gel Electrophoresis ◽  
Transfection Efficiency ◽  
Carcinoma Cells ◽  
Molar Ratio ◽  
Dna Complexes ◽  
Human Carcinoma

Polyethylenimine (PEI) was modified by cholic acid at a molar ratio of 1:1. Cholic acid (CA)-modified PEI (PEI-CA) were evaluated for formation of DNA complexes. PEI-CA/pEGFP plasmid DNA complexes were characterized for their size and zeta potential. Gel electrophoresis showed total retardation for PEI-CA/pEGFP complexes formed at weight ratios above 0.25. The particle size and zeta potential of the complexes at a polymer-to-DNA ratio of 0.5 were 295.3 nm and 30.5 mV, respectively. The transfection efficiency of PEI-CA/pEGFP complexes was comparable to unmodified PEI. Cytotoxicity result showed that PEI-CA had lower cytoxicity than PEI. This study suggests that PEI-CA has potential utility as a gene delivery carrier.

Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing

2017 ◽  
Vol 5 (11) ◽  
pp. 2328-2336 ◽  
Author(s):  
Mathias Dimde ◽  
Falko Neumann ◽  
Felix Reisbeck ◽  
Svenja Ehrmann ◽  
Jose Luis Cuellar-Camacho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
Genetic Material ◽  
Ph Sensitive ◽  
Carrier System ◽  
High Transfection Efficiency ◽  
Delivery Platform ◽  
Low Cytotoxicity

An advanced cationic carrier system which combines high transfection efficiency with low cytotoxicity and a control over the release of the encapsulated genetic material by the reduction of the multivalent architecture upon pH triggered degradation was developed.

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

2016 ◽  
Vol 4 (39) ◽  
pp. 6462-6467 ◽  
Author(s):  
Sheng-Gang Ding ◽  
Lei Yu ◽  
Long-Hai Wang ◽  
Lin-Ding Wang ◽  
Zhi-Qiang Yu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Dendritic Polymer ◽  
Self Assembling ◽  
High Gene ◽  
Low Cytotoxicity

Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery.

Inducing Tumor Cell Apoptosis: Mediated Survivin miRNA by Ultrasound and Cationic Lipid Contrast Agent

2020 ◽  
Vol 20 ◽  
Author(s):  
Feng Yang ◽  
Liu-Fu Chun ◽  
Zhi-Yi Chen
Keyword(s):  
Drug Delivery ◽  
Contrast Agent ◽  
Hela Cells ◽  
Cell Apoptosis ◽  
Transfection Efficiency ◽  
Ultrasound Contrast Agent ◽  
Chemical Properties ◽  
Cationic Lipid ◽  
Viral Gene ◽  
Ultrasound Contrast

Background: Non-viral delivery systems is a promising method for gene or drug delivery. Polyethyleneimine(PEI) is a double-edged sword. It internalizes itself into the cell through endocytosis and promotes gene transfer efficiency. However, the large positive charge also makes PEI highly toxic to cells. Ultrasound-targeted microbubble destruction (UTMD) is a promising non-viral method for gene and drug delivery, but its efficiency still needs to be improved. Objective: The aim of this study was to explore a system that combines ultrasound with non-viral gene delivery for the treatment of cervical cancer HeLa cells. Methods: In this study, we synthesized a kind of cationic ultrasound contrast agent(C-UCA)that the physical and chemical properties, gene carrying capacity and cytotoxicity were verified. On the basis of previous studies, we further optimized the following transfusion parameters including ultrasound parameters, microbubble concentration, plasmid concentration, cell density and other parameters. The experiment was designed to compare the following six groups: (1) Plasmid group (P group), plasmid 15 μg; (2) PEI + plasmid group (PEI + P group),1 μl of PEI containing 10 nmol nitrogen and 1 μg of DNA containing 3 nmol phosphate for a PEI/DNA ratio equal to a nitrogen/phosphate ratio of 7 for transfection; (3) Ultrasound + plasmid group (US + P), plasmid 15 µg; (4) Ultrasound + cationic liposomal ultrasound contrast agent + plasmid group (UTMD + P group), plasmid 15 µg and cationic liposomal ultrasound contrast agent 5%; (5) Ultrasound + cationic liposomal ultrasound contrast agent + PEI + plasmid group (UTMD + PEI + P group), The influence on Hela cells was observed under microscope, the efficiency of apoptosis was measured by flow cytometry, and cell viability was tested in CCK 8. Results: The optimized transfection parameters can improve the transfection efficiency of ultrasound combined with CUCA to a certain extent, but its transfection efficiency is still lower than that of branched polyethyleneimine (bPEI) 25 kDa. By investigating the effect of HeLa cells apoptosis induced by UTMD in combination with PEI mediated survivin miRNA, we found that both PEI alone and ultrasound in combination with C-UCA were able to transfect cells with survivin miRNA to effectively induce HeLa cell apoptosis. However, the synergistic effect between the two methods was not significant. Conclusion: In contrast, the combined use of ultrasound, C-UCA and PEI may significantly reduce the transfection efficiency of UTMD and PEI, and the specific mechanism remains to be further studied.

Development of new self-assembled cationic amino liposomes for efficient gene delivery

2020 ◽  
Vol 8 (11) ◽  
pp. 3021-3025 ◽  
Author(s):  
Yihang Wu ◽  
Yue Xiong ◽  
Ling Wang ◽  
Quanming Zhou ◽  
Linxian Li ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Self Assembling ◽  
Efficient Gene ◽  
Self Assembled ◽  
Low Cytotoxicity

A library of lipidoids self-assembling to liposomes exhibits excellent transfection efficiency in HEK 293T cells and mESCs with low cytotoxicity.

Niosomes Containing Spermine-Based Cationic Lipid with Different Linkers for siRNA Delivery

2019 ◽  
Vol 819 ◽  
pp. 169-174
Author(s):  
Supusson Pengnam ◽  
Praneet Opanasopit ◽  
Theerasak Rojanarata ◽  
Nattisa Ni-yomtham ◽  
Boon Ek Yingyongnarongkul ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Sirna Delivery ◽  
Weight Ratio ◽  
Cationic Lipid ◽  
Cationic Lipids ◽  
Molar Ratio ◽  
Ionic Surfactant ◽  
Low Toxicity ◽  
Cationic Niosomes

Niosomes are a lipid nanoparticle which have been widely used as non-viral carrier for therapeutic DNA or siRNA. They are formulated from non-ionic surfactant and other helper lipids. The aim of this study were to formulate niosome containing spermine-based cationic lipid with different linkers and to evaluate the efficiency of siRNA delivery in cervical cancer cell (HeLa cell). The niosomes were formulated from cholesterol (Chol), Span 20 and different cationic lipid (Ay, By, Cy and Dy) at various molar ratios. The properties of niosomes and ability of niosome to complex with siRNA were characterized. The cellular uptake, gene silencing efficiency and cytotoxicity were also determined. From the results, niosomes formulated at Chol:Span20:lipid molar ratio of 2.5:2.5:2 showed positive zeta potential and they were in nanosize (<200 nm). The binding ability of cationic niosomes to siRNA depended on types of cationic lipid. Among niosome/siRNA complexes, the niosome By/siRNA complex provided the highest gene silencing efficiency at weight ratio of 20. The highest cellular uptake also obtained by using niosome By as a carrier. The cytotoxicity revealed that cationic niosomes had low toxicity (cell viability > 80%). In conclusion, the cationic niosomes prepared from Chol, Span 20 and spermine-based cationic lipids are able to complex with siRNA and suitable for siRNA delivery with low toxicity.

scholarly journals Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 696
Author(s):  
Yerai Vado ◽  
Gustavo Puras ◽  
Melania Rosique ◽  
Cesar Martin ◽  
Jose Luis Pedraz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Intracellular Localization ◽  
Cell Types ◽  
Functional Studies ◽  
Cellular Models ◽  
Cationic Niosomes

Background: Mesenchymal stem cells (MSCs) are stem cells present in adult tissues. They can be cultured, have great growth capacity, and can differentiate into several cell types. The isolation of urine-derived mesenchymal stem cells (hUSCs) was recently described. hUSCs present additional benefits in the fact that they can be easily obtained noninvasively. Regarding gene delivery, nonviral vectors based on cationic niosomes have been used and are more stable and have lower immunogenicity than viral vectors. However, their transfection efficiency is low and in need of improvement. Methods: We isolated hUSCs from urine, and the cell culture was tested and characterized. Different cationic niosomes were elaborated using reverse-phase evaporation, and they were physicochemically characterized. Then, they were screened into hUSCs for transfection efficiency, and their internalization was evaluated. Results: GPxT-CQ at a lipid/DNA ratio of 5:1 (w/w) had the best transfection efficiency. Intracellular localization studies confirmed that nioplexes entered mainly via caveolae-mediated endocytosis. Conclusions: In conclusion, we established a protocol for hUSC isolation and their transfection with cationic niosomes, which could have relevant clinical applications such as in gene therapy. This methodology could also be used for creating cellular models for studying and validating pathogenic genetic variants, and even for performing functional studies. Our study increases knowledge about the internalization of tested cationic niosomes in these previously unexplored cells.

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