The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin
Keyword(s):
In Britton Robinson (B-R) buffer solution with pH = 7.00 and sodium dodecyl sulfate (SDS) medium, an effective energy transfer between acridine orange (AO) and Rhodamine B (RB) can occur, which can enhance the fluorescence of RB. The addition of Gemifloxacin (GMFX) can quench the fluorescence of RB. So a new method for the indirect determination of Gemifloxacin was established by AO-RB fluorescence resonance energy transfer. This method was applied to the determination of Gemifloxacin tablets. The results were consistent with those of high performance liquid chromatography (HPLC) . Experiments show that this method is simple, rapid, accurate and sensitive.
2000 ◽
Vol 275
(47)
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pp. 37048-37054
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2021 ◽
pp. 120643
2014 ◽
Vol 61
(6)
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pp. 638-642
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