In Vitro Wound Healing Effect of Asiaticoside Extracted from Centella asiatica (‘Pegaga’) on Human Gingival Fibroblast Cell Line

2021 ◽  
Vol 1025 ◽  
pp. 224-229
Author(s):  
Wastuti Hidayati Suriyah ◽  
Aisyah Juares Rizal ◽  
Hana Syakirah Mohamed Nadzirin ◽  
Solachuddin Jauhari Arief Ichwan ◽  
Muhammad Lokman Md. Isa
Keyword(s):  
Wound Healing ◽  
Mtt Assay ◽  
Centella Asiatica ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Potential Candidate ◽  
Scratch Assay ◽  
Qrt Pcr ◽  
Healing Effect

Asiaticoside is a bioactive compound found in the traditional plant Centella asiatica (Asiatic pennywort or ‘Pegaga’) generally used for wound healing applications. Numerous studies have discussed the potential benefits of asiaticoside on different human cells such as keratinocytes and dermal fibroblast cells in healing of wounds. However only very few studies have been conducted to investigate its healing effect on cells originated from human oral cavity. The present study aimed to determine the potential of asiaticoside on human gingival fibroblast cells. Cytotoxic activities of the compounds were assessed by MTT assay. The wound healing was examined by scratch assay. The effect of asiaticoside on Col1A1 gene expression was also analyzed using qRT-PCR. Col1A1 is known to play a crucial role in wound healing. The MTT assay result showed that the maximum tolerable concentration of asiaticoside was 0.25 mg/ml. The scratch assay revealed that asiaticoside significantly accelerated the wound healing compared to the negative control (P<0.05). Moreover, the qRT-PCR demonstrated that asiaticoside markedly increased Col1A1 mRNA expression. These results proved asiaticoside as a potential candidate for wound healing agent in dentistry.

The effects of ozone application on genotoxic damage and wound healing in bisphosphonate-applied human gingival fibroblast cells

2017 ◽  
Vol 22 (2) ◽  
pp. 867-873 ◽  
Author(s):  
Sıdıka Sinem Akdeniz ◽  
E. Beyler ◽  
Y. Korkmaz ◽  
E. Yurtcu ◽  
U. Ates ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Genotoxic Damage

scholarly journals Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 138 ◽  
Author(s):  
Chung-Chih Tseng ◽  
Yu-Cheng Lai ◽  
Tsu-Jen Kuo ◽  
Jui-Hsin Su ◽  
Ping-Jyun Sung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Oxidative Phosphorylation ◽  
Marine Biodiversity ◽  
Human Gingival Fibroblast ◽  
Type I ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Expression Levels ◽  
Gene And Protein Expression

Gingival recession (GR) potentially leads to the exposure of tooth root to the oral cavity microenvironment and increases susceptibility to dental caries, dentin hypersensitivity, and other dental diseases. Even though many etiological factors were reported, the specific mechanism of GR is yet to be elucidated. Given the species richness concerning marine biodiversity, it could be a treasure trove for drug discovery. In this study, we demonstrate the effects of a marine compound, (+)-rhodoptilometrin from crinoid, on gingival cell migration, wound healing, and oxidative phosphorylation (OXPHOS). Experimental results showed that (+)-rhodoptilometrin can significantly increase wound healing, migration, and proliferation of human gingival fibroblast cells, and it does not have effects on oral mucosa fibroblast cells. In addition, (+)-rhodoptilometrin increases the gene and protein expression levels of focal adhesion kinase (FAK), fibronectin, and type I collagen, changes the intracellular distribution of FAK and F-actin, and increases OXPHOS and the expression levels of complexes I~V in the mitochondria. Based on our results, we believe that (+)-rhodoptilometrin might increase FAK expression and promote mitochondrial function to affect cell migration and promote gingival regeneration. Therefore, (+)-rhodoptilometrin may be a promising therapeutic agent for GR.

scholarly journals In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

2019 ◽  
Vol 33 ◽  
Author(s):  
Cláudio Rodrigues Rezende Costa ◽  
Bruna Rabelo Amorim ◽  
Sandra Márcia Mazutti da Silva ◽  
Ana Carolina Acevedo ◽  
Pérola de Oliveira Magalhães ◽  
...  
Keyword(s):  
Primary Culture ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Evaluation ◽  
Eugenia Dysenterica

The Application of a Recombinant Antimicrobial Peptide of Thrombocidin-1 expressed in Pichia pastoris as a Novel Approach Against Some Oral Pathogenic Bacteria: An In-vitro Study

2021 ◽  
Vol 28 ◽  
Author(s):  
Fatemeh Forouzanfar ◽  
Hamideh Sadat Mohammadipour ◽  
Majid Akbari ◽  
Reza Beyraghshamshir ◽  
Abbas Tanhaeian ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Dilution Method ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Oral Infections ◽  
Oral Pathogens

Objective: Oral infections and dental caries are considered serious health problems. Therefore, searching for new agents with antimicrobial properties seems to be crucial. This study aimed to evaluate the antimicrobial activity of the recombinant Thrombocidin-1 [TC-1] peptide on some oral pathogens. Also, the cytotoxicity of this peptide on human gingival fibroblast cells was investigated. Methods & Materials: In this study, Pichia pastoris was used for the expression of recombinant TC-1. The microbroth dilution method was used to determine the minimum inhibitory concentration [MIC] and minimum bacterial concentration [MBC]. It tested against four main oral pathogens; Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis, and Enterococcus faecalis. Moreover, the cytotoxicity analysis was done on gingival fibroblast cells by the MTT method. The data were analyzed using a two-way analysis of variance [ANOVA] and Tukey’s HSD tests. Results: The most bactericidal effect of TC-1 was against S. salivarius, the highest bacteriostatic effect was against S. salivarius, and S. oralis had the lowest MIC value of 1.512 μg/ml. The Thrombocidin-1 peptide showed lower antibacterial properties against E. faecalis compared with CHX, unlike the stronger antimicrobial effect on examined streptococci. According to cytotoxicity examination, no concentration of TC-1 presented over 50% growth inhibition [IC50] of the fibroblasts cells. Conclusion: Based on antimicrobial tests and cytotoxicity results, the Thrombocidin-1 peptide may be useful as a safe antibacterial agent against some oral pathogens in dental materials.

In vitro effects of ascorbic acid and β-glycerophosphate on human gingival fibroblast cells

2012 ◽  
Vol 44 (5) ◽  
pp. 325-331 ◽  
Author(s):  
Elizabeth F. Martinez ◽  
Tatiani A.G. Donato ◽  
Victor E. Arana-Chavez
Keyword(s):  
Ascorbic Acid ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Effects

Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts

2009 ◽  
Vol 126 (3) ◽  
pp. 463-467 ◽  
Author(s):  
M. Fronza ◽  
B. Heinzmann ◽  
M. Hamburger ◽  
S. Laufer ◽  
I. Merfort
Keyword(s):  
Wound Healing ◽  
Scratch Assay ◽  
Healing Effect ◽  
3T3 Fibroblasts ◽  
Wound Healing Effect

scholarly journals Ulmus Parvifolia Accelerate Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

2019 ◽  
Author(s):  
Min Cheol Kang ◽  
Silvia Yumnam ◽  
Woo Sung Park ◽  
Hae Min So ◽  
Ki Hyun Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Skin Wound ◽  
Root Bark ◽  
Potential Candidate ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Ulmus Parvifolia ◽  
Wound Healing Effect

Ulmus species have been widely used in Korean folk medicine because of their anti-inflammatory and antimicrobial properties. We intended to investigate the wound healing effect of the powder of Ulmus parvifolia (UP) root bark in a mouse wound healing model. We also determined the mechanisms of effects of Ulmus parvifolia (UP) in skin and skin wound healing effect using keratinocyte model. in vivo experiments showed that the wound lesions in the mice decreased by U. parvifolia with 200 mesh size of root bark powder and significantly reduced by treatment with UP, compared with those treated with U. macrocarpa (UM). Results from in vitro experiments also revealed that UP extract promoted the migration of human skin keratinocytes. UP powder treatment upregulated the expression of the matrix metalloproteinase-2 and -9 protein and significantly increased transforming growth factor (TGF)-&beta; levels. We confirmed that topical administration of the bark powder of exerted a significant effect on skin wound healing by upregulating the expression of MMP and transforming growth factor-&beta;. TGF-&beta; In, Our study suggests that U. parvifolia may be a potential candidate for skin wound healing including epidermal skin rejuvernation.

scholarly journals Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts

2016 ◽  
Vol 5 (2) ◽  
pp. 83-88
Author(s):  
Thuy Anh Vu Pham ◽  
Hao TT Nguyen ◽  
My TN Nguyen ◽  
Van NL Trinh ◽  
Nga Y Tran ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Gingival Fibroblasts ◽  
Human Gingival Fibroblast ◽  
Complete Medium ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Platelet Rich Fibrin ◽  
Liquid Extract ◽  
And Migration ◽  
Proliferation And Migration

ABSTRACT Aims Our study focused on the fabrication of platelet-rich fibrin (PRF) and evaluated its influences on cell behaviors, including proliferation and migration. Materials and methods Platelet-rich fibrin was prepared from human peripheral blood according to Choukroun's method without using nonanticoagulant and foreign factors for platelet activation. Platelet-rich fibrin architecture was studied by hematoxylin and eosin staining. The investigation of PRF effects on human gingival fibroblasts (hGFs) was conducted via PRF liquid extract. Cell proliferation was determined via the number of cells after a period of time incubated in PRF liquid extract. Influence of PRF liquid extract on the migration of hGFs was conducted via scratch wound healing assay. Results Histological staining reviewed the natural fibrin fiber matrix of PRF. Platelet-rich fibrin liquid extract promoted hGF proliferation after 7 days of cultivation. Human gingival fibroblast proliferation in PRF liquid extract was more superior than those cultured in complete medium. Platelet-rich fibrin was also found to be able to promote the migration of hGFs for up to 48 hours. Conclusion These results indicated that PRF is suitable to be used as autologous natural biomaterial in supporting wound healing and in further application in periodontitis treatments. How to cite this article Nguyen HTT, Nguyen MTN, Trinh VNL, Tran NY, Ngo LTQ, Pham TAV, Tran HLB. Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts. Int J Experiment Dent Sci 2016;5(2):83-88.

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