scholarly journals Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 138 ◽  
Author(s):  
Chung-Chih Tseng ◽  
Yu-Cheng Lai ◽  
Tsu-Jen Kuo ◽  
Jui-Hsin Su ◽  
Ping-Jyun Sung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Oxidative Phosphorylation ◽  
Marine Biodiversity ◽  
Human Gingival Fibroblast ◽  
Type I ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Expression Levels ◽  
Gene And Protein Expression

Gingival recession (GR) potentially leads to the exposure of tooth root to the oral cavity microenvironment and increases susceptibility to dental caries, dentin hypersensitivity, and other dental diseases. Even though many etiological factors were reported, the specific mechanism of GR is yet to be elucidated. Given the species richness concerning marine biodiversity, it could be a treasure trove for drug discovery. In this study, we demonstrate the effects of a marine compound, (+)-rhodoptilometrin from crinoid, on gingival cell migration, wound healing, and oxidative phosphorylation (OXPHOS). Experimental results showed that (+)-rhodoptilometrin can significantly increase wound healing, migration, and proliferation of human gingival fibroblast cells, and it does not have effects on oral mucosa fibroblast cells. In addition, (+)-rhodoptilometrin increases the gene and protein expression levels of focal adhesion kinase (FAK), fibronectin, and type I collagen, changes the intracellular distribution of FAK and F-actin, and increases OXPHOS and the expression levels of complexes I~V in the mitochondria. Based on our results, we believe that (+)-rhodoptilometrin might increase FAK expression and promote mitochondrial function to affect cell migration and promote gingival regeneration. Therefore, (+)-rhodoptilometrin may be a promising therapeutic agent for GR.

The effects of ozone application on genotoxic damage and wound healing in bisphosphonate-applied human gingival fibroblast cells

2017 ◽  
Vol 22 (2) ◽  
pp. 867-873 ◽  
Author(s):  
Sıdıka Sinem Akdeniz ◽  
E. Beyler ◽  
Y. Korkmaz ◽  
E. Yurtcu ◽  
U. Ates ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Genotoxic Damage

scholarly journals In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Fernanda G. Basso ◽  
Taisa N. Pansani ◽  
Ana Paula S. Turrioni ◽  
Vanderlei S. Bagnato ◽  
Josimeri Hebling ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Cell Metabolism ◽  
Statistical Significance ◽  
Cell Number ◽  
Nonparametric Tests ◽  
Low Level Laser Therapy ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast

The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3×104cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hour incubation with 5% CO2at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable;780±3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2. Cells were irradiated every 24 h totalizing 3 applications. Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay). Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%. Irradiation of the fibroblasts with 0.5 and 3 J/cm2resulted in significant increase in cell metabolism compared with the nonrradiated group (P<0.05). Both energy doses promoted significant increase in the cell number as well as in cell migration (P<0.05). These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.

In Vitro Wound Healing Effect of Asiaticoside Extracted from Centella asiatica (‘Pegaga’) on Human Gingival Fibroblast Cell Line

2021 ◽  
Vol 1025 ◽  
pp. 224-229
Author(s):  
Wastuti Hidayati Suriyah ◽  
Aisyah Juares Rizal ◽  
Hana Syakirah Mohamed Nadzirin ◽  
Solachuddin Jauhari Arief Ichwan ◽  
Muhammad Lokman Md. Isa
Keyword(s):  
Wound Healing ◽  
Mtt Assay ◽  
Centella Asiatica ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Potential Candidate ◽  
Scratch Assay ◽  
Qrt Pcr ◽  
Healing Effect

Asiaticoside is a bioactive compound found in the traditional plant Centella asiatica (Asiatic pennywort or ‘Pegaga’) generally used for wound healing applications. Numerous studies have discussed the potential benefits of asiaticoside on different human cells such as keratinocytes and dermal fibroblast cells in healing of wounds. However only very few studies have been conducted to investigate its healing effect on cells originated from human oral cavity. The present study aimed to determine the potential of asiaticoside on human gingival fibroblast cells. Cytotoxic activities of the compounds were assessed by MTT assay. The wound healing was examined by scratch assay. The effect of asiaticoside on Col1A1 gene expression was also analyzed using qRT-PCR. Col1A1 is known to play a crucial role in wound healing. The MTT assay result showed that the maximum tolerable concentration of asiaticoside was 0.25 mg/ml. The scratch assay revealed that asiaticoside significantly accelerated the wound healing compared to the negative control (P<0.05). Moreover, the qRT-PCR demonstrated that asiaticoside markedly increased Col1A1 mRNA expression. These results proved asiaticoside as a potential candidate for wound healing agent in dentistry.

scholarly journals In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

2019 ◽  
Vol 33 ◽  
Author(s):  
Cláudio Rodrigues Rezende Costa ◽  
Bruna Rabelo Amorim ◽  
Sandra Márcia Mazutti da Silva ◽  
Ana Carolina Acevedo ◽  
Pérola de Oliveira Magalhães ◽  
...  
Keyword(s):  
Primary Culture ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Evaluation ◽  
Eugenia Dysenterica

The Application of a Recombinant Antimicrobial Peptide of Thrombocidin-1 expressed in Pichia pastoris as a Novel Approach Against Some Oral Pathogenic Bacteria: An In-vitro Study

2021 ◽  
Vol 28 ◽  
Author(s):  
Fatemeh Forouzanfar ◽  
Hamideh Sadat Mohammadipour ◽  
Majid Akbari ◽  
Reza Beyraghshamshir ◽  
Abbas Tanhaeian ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Dilution Method ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Oral Infections ◽  
Oral Pathogens

Objective: Oral infections and dental caries are considered serious health problems. Therefore, searching for new agents with antimicrobial properties seems to be crucial. This study aimed to evaluate the antimicrobial activity of the recombinant Thrombocidin-1 [TC-1] peptide on some oral pathogens. Also, the cytotoxicity of this peptide on human gingival fibroblast cells was investigated. Methods & Materials: In this study, Pichia pastoris was used for the expression of recombinant TC-1. The microbroth dilution method was used to determine the minimum inhibitory concentration [MIC] and minimum bacterial concentration [MBC]. It tested against four main oral pathogens; Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis, and Enterococcus faecalis. Moreover, the cytotoxicity analysis was done on gingival fibroblast cells by the MTT method. The data were analyzed using a two-way analysis of variance [ANOVA] and Tukey’s HSD tests. Results: The most bactericidal effect of TC-1 was against S. salivarius, the highest bacteriostatic effect was against S. salivarius, and S. oralis had the lowest MIC value of 1.512 μg/ml. The Thrombocidin-1 peptide showed lower antibacterial properties against E. faecalis compared with CHX, unlike the stronger antimicrobial effect on examined streptococci. According to cytotoxicity examination, no concentration of TC-1 presented over 50% growth inhibition [IC50] of the fibroblasts cells. Conclusion: Based on antimicrobial tests and cytotoxicity results, the Thrombocidin-1 peptide may be useful as a safe antibacterial agent against some oral pathogens in dental materials.

In vitro effects of ascorbic acid and β-glycerophosphate on human gingival fibroblast cells

2012 ◽  
Vol 44 (5) ◽  
pp. 325-331 ◽  
Author(s):  
Elizabeth F. Martinez ◽  
Tatiani A.G. Donato ◽  
Victor E. Arana-Chavez
Keyword(s):  
Ascorbic Acid ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Effects

scholarly journals Hyaluronate Acid-Dependent Protection and Enhanced Corneal Wound Healing against Oxidative Damage in Corneal Epithelial Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Jing Zhong ◽  
Yuqing Deng ◽  
Bishan Tian ◽  
Bowen Wang ◽  
Yifang Sun ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Apoptosis ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial Cells ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Expression Levels ◽  
Corneal Wound

Purpose. To evaluate the effects and mechanism of exogenous hyaluronate (HA) in promoting corneal wound healing.Methods. Human corneal epithelial cells (HCECs) were incubated with different concentrations of HA to evaluate their efficiency in promoting cell migration and their modulation of repair factors. After inducing hyperosmolar conditions, the cell morphologies, cell apoptosis, and expression levels of TNF-αand MMP-9 were detected to assess the protective role of HA. Corneal epithelium-injured rat models were established to test the therapeutic effects of 0.3% HA. Then, the wound healing rates, the RNA expression levels of inflammatory cytokines, and repair factors were examined.Results. HCECs in the 0.03% and 0.3% HA groups showed fewer morphological alterations and lower rates of cell apoptosis following preincubation with HA under hyperosmolar conditions, as well as the expression levels of MMP-9 and TNF-α. In the rat model, the areas of fluorescein staining in the corneas of 0.3% HA group were significantly smaller than the control group. The expression levels of IL-1βand MMP-9 were decreased, while CD44 and FN were increased in the 0.3% HA group.Conclusion. HA enhanced corneal epithelial cell wound healing by promoting cell migration, upregulating repair responses, and suppressing inflammatory responses.

scholarly journals Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts

2016 ◽  
Vol 5 (2) ◽  
pp. 83-88
Author(s):  
Thuy Anh Vu Pham ◽  
Hao TT Nguyen ◽  
My TN Nguyen ◽  
Van NL Trinh ◽  
Nga Y Tran ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Gingival Fibroblasts ◽  
Human Gingival Fibroblast ◽  
Complete Medium ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Platelet Rich Fibrin ◽  
Liquid Extract ◽  
And Migration ◽  
Proliferation And Migration

ABSTRACT Aims Our study focused on the fabrication of platelet-rich fibrin (PRF) and evaluated its influences on cell behaviors, including proliferation and migration. Materials and methods Platelet-rich fibrin was prepared from human peripheral blood according to Choukroun's method without using nonanticoagulant and foreign factors for platelet activation. Platelet-rich fibrin architecture was studied by hematoxylin and eosin staining. The investigation of PRF effects on human gingival fibroblasts (hGFs) was conducted via PRF liquid extract. Cell proliferation was determined via the number of cells after a period of time incubated in PRF liquid extract. Influence of PRF liquid extract on the migration of hGFs was conducted via scratch wound healing assay. Results Histological staining reviewed the natural fibrin fiber matrix of PRF. Platelet-rich fibrin liquid extract promoted hGF proliferation after 7 days of cultivation. Human gingival fibroblast proliferation in PRF liquid extract was more superior than those cultured in complete medium. Platelet-rich fibrin was also found to be able to promote the migration of hGFs for up to 48 hours. Conclusion These results indicated that PRF is suitable to be used as autologous natural biomaterial in supporting wound healing and in further application in periodontitis treatments. How to cite this article Nguyen HTT, Nguyen MTN, Trinh VNL, Tran NY, Ngo LTQ, Pham TAV, Tran HLB. Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts. Int J Experiment Dent Sci 2016;5(2):83-88.

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