scholarly journals Ulmus Parvifolia Accelerate Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

2019 ◽  
Author(s):  
Min Cheol Kang ◽  
Silvia Yumnam ◽  
Woo Sung Park ◽  
Hae Min So ◽  
Ki Hyun Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Skin Wound ◽  
Root Bark ◽  
Potential Candidate ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Ulmus Parvifolia ◽  
Wound Healing Effect

Ulmus species have been widely used in Korean folk medicine because of their anti-inflammatory and antimicrobial properties. We intended to investigate the wound healing effect of the powder of Ulmus parvifolia (UP) root bark in a mouse wound healing model. We also determined the mechanisms of effects of Ulmus parvifolia (UP) in skin and skin wound healing effect using keratinocyte model. in vivo experiments showed that the wound lesions in the mice decreased by U. parvifolia with 200 mesh size of root bark powder and significantly reduced by treatment with UP, compared with those treated with U. macrocarpa (UM). Results from in vitro experiments also revealed that UP extract promoted the migration of human skin keratinocytes. UP powder treatment upregulated the expression of the matrix metalloproteinase-2 and -9 protein and significantly increased transforming growth factor (TGF)-β levels. We confirmed that topical administration of the bark powder of exerted a significant effect on skin wound healing by upregulating the expression of MMP and transforming growth factor-β. TGF-β In, Our study suggests that U. parvifolia may be a potential candidate for skin wound healing including epidermal skin rejuvernation.

scholarly journals Ulmus parvifolia Accelerates Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

2019 ◽  
Vol 9 (1) ◽  
pp. 59
Author(s):  
Min Cheol Kang ◽  
Silvia Yumnam ◽  
Woo Sung Park ◽  
Hae Min So ◽  
Ki Hyun Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Skin Wound ◽  
Matrix Metalloproteinase 2 ◽  
Root Bark ◽  
Potential Candidate ◽  
Skin Wound Healing ◽  
Ulmus Parvifolia ◽  
Model Animal

Ulmus parvifolia is one of the medicinal plants used traditionally for treatment of wounds. We intended to investigate the wound healing effect of the powder of Ulmus parvifolia (UP) root bark in a mouse wound healing model. We also determined the mechanisms of effects of U. parvifolia in skin and skin wound healing effects using a keratinocyte model. Animal experiments showed that the wound lesions in the mice decreased with 200 mesh U. parvifolia root bark powder and were significantly reduced with treatment by UP, compared with those treated with Ulmus macrocarpa (UM). Results from in vitro experiments also revealed that UP extract promoted the migration of human skin keratinocytes. UP powder treatment upregulated the expression of the matrix metalloproteinase-2 and -9 protein and significantly increased transforming growth factor (TGF)-β levels. We confirmed that topical administration of the bark powder exerted a significant effect on skin wound healing by upregulating the expression of MMP and transforming growth factor-β. Our study suggests that U. parvifolia may be a potential candidate for skin wound healing including epidermal skin rejuvenation.

scholarly journals From Inflammation to Cutaneous Repair: Topical Application of Lupeol Improves Skin Wound Healing in Rats by Modulating the Cytokine Levels, NF-κB, Ki-67, Growth Factor Expression, and Distribution of Collagen Fibers

2020 ◽  
Vol 21 (14) ◽  
pp. 4952 ◽  
Author(s):  
Fernando Pereira Beserra ◽  
Lucas Fernando Sérgio Gushiken ◽  
Ana Júlia Vieira ◽  
Danilo Augusto Bérgamo ◽  
Patrícia Luísa Bérgamo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Growth Factor ◽  
Skin Wound ◽  
Collagen Fibers ◽  
Skin Wound Healing ◽  
Ki 67 ◽  
Healing Effect ◽  
Cytokine Levels ◽  
Wound Healing Effect

Skin wound healing is a highly complex event that involves different mediators at the cellular and molecular level. Lupeol has been reported to possess different biological activities, such as anti-inflammatory, antioxidant, antidiabetic, and in vitro wound healing properties, which motivated us to proceed with in vivo studies. We aimed to investigate the wound healing effect of lupeol-based cream for 3, 7, and 14 days. Wound excisions were induced on the thoraco-lumbar region of rats and topically treated immediately after injury induction. Macroscopic, histopathological, and immunohistochemical analyses were performed. Cytokine levels were measured by ELISA and gene expression was evaluated by real-time RT-qPCR. Our results showed a strong wound-healing effect of lupeol-based cream after 7 and 14 days. Lupeol treatment caused a reduction in proinflammatory cytokines (TNF-a, IL-1β, and IL-6) and gene and protein NF-κB expression, and positively altered IL-10 levels, showing anti-inflammatory effects in the three treatment periods. Lupeol treatment showed involvement in the proliferative phase by stimulating the formation of new blood vessels, increasing the immunostaining of Ki-67 and gene expression, and immunolabeling of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF), and increasing gene expression of transforming growth factor beta-1 (TGF-β1) after seven days of treatment. Lupeol was also involved in the tissue regeneration phase by increasing the synthesis of collagen fibers noted in the three treatment periods analyzed. Our findings suggest that lupeol may serve as a novel therapeutic option to treat cutaneous wounds by regulating mechanisms involved in the inflammatory, proliferative, and tissue-remodeling phases.

Myostatin-null mice exhibit delayed skin wound healing through the blockade of transforming growth factor-β signaling by decorin

2012 ◽  
Vol 302 (8) ◽  
pp. C1213-C1225 ◽  
Author(s):  
Chen Zhang ◽  
Chek Kun Tan ◽  
Craig McFarlane ◽  
Mridula Sharma ◽  
Nguan Soon Tan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Critical Role ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Skin Wound Healing

Myostatin (Mstn) is a secreted growth and differentiation factor that belongs to the transforming growth factor-β (TGF-β) superfamily. Mstn has been well characterized as a regulator of myogenesis and has been shown to play a critical role in postnatal muscle regeneration. Herein, we report for the first time that Mstn is expressed in both epidermis and dermis of murine and human skin and that Mstn-null mice exhibited delayed skin wound healing attributable to a combination of effects resulting from delayed epidermal reepithelialization and dermal contraction. In epidermis, reduced keratinocyte migration and protracted keratinocyte proliferation were observed, which subsequently led to delayed recovery of epidermal thickness and slower reepithelialization. Furthermore, primary keratinocytes derived from Mstn-null mice displayed reduced migration capacity and increased proliferation rate as assessed through in vitro migration and adhesion assays, as well as bromodeoxyuridine incorporation and Western blot analysis. Moreover, in dermis, both fibroblast-to-myofibroblast transformation and collagen deposition were concomitantly reduced, resulting in a delayed dermal wound contraction. These decreases are due to the inhibition of TGF-β signaling. In agreement, the expression of decorin, a naturally occurring TGF-β suppressor, was elevated in Mstn-null mice; moreover, topical treatment with TGF-β1 protein rescued the impaired skin wound healing observed in Mstn-null mice. These observations highlight the interplay between TGF-β and Mstn signaling pathways, specifically through Mstn regulation of decorin levels during the skin wound healing process. Thus we propose that Mstn agonists might be beneficial for skin wound repair.

Mesenchymal stem cells' interaction with skin: Wound-healing effect on fibroblast cells and skin tissue

2010 ◽  
Vol 18 (6) ◽  
pp. 655-661 ◽  
Author(s):  
Young Keul Jeon ◽  
Yun Ho Jang ◽  
Dong Ryeol Yoo ◽  
Si Na Kim ◽  
Sang Koo Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Skin Tissue ◽  
Fibroblast Cells ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Wound Healing Effect

scholarly journals 4-aminopyridine promotes accelerated skin wound healing

2021 ◽  
Author(s):  
Jagadeeshaprasad Mashanipalya ◽  
Prem Kumar Govindappa ◽  
Amanda Nelson ◽  
Mark Noble ◽  
John Elfar
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wound Closure ◽  
Transforming Growth Factor ◽  
Smooth Muscle Actin ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
S 100 ◽  
Skin Healing

Abstract The discovery of ways to enhance skin healing is of great importance due to the frequency and severity of skin wounds. We discovered that 4-aminopyridine (4-AP), a potassium channel blocker, greatly enhances skin wound healing. Benefits include faster wound closure, restoration of normal-appearing skin architecture and epidermal thickness, increased vascularization and increases in K14+ keratinocytes. Hair follicle number was increased, both histologically and by analysis of K15 and K17 expression. Levels of vimentin (which marks fibroblasts) and α-smooth muscle actin (α-SMA, which marks collagen-producing myofibroblasts) increased, as did α-SMA+ cell numbers. 4-AP also increased numbers of axons and S-100+ Schwann cells, and increased expression of p75-NTR and SOX10. Treatment also increased levels of nerve growth factor, transforming growth factor-β, Substance P and PGP9.5, important modulators of wound healing. As 4-AP is already used for treatment of multiple sclerosis and other chronic neurological syndromes, it has strong potential for rapid translational development.

Wound healing effect benzosulfonate 1-ethyl-3-methyl-4,5-bis(n-methylcarbamoyl) imidazolium

2020 ◽  
Vol 18 (3) ◽  
pp. 229-235
Author(s):  
Lydmila K. Khnychenko ◽  
Elena N. Selina ◽  
Olga M. Rodionova ◽  
Levon B. Piotrovskiy ◽  
Petr D. Shabanov
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Experimental Models ◽  
Skin Wound ◽  
Complete Healing ◽  
Male Rats ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Wound Healing Effect

Materials and methods. In experiments on 180 male rats weighing 180200 g, the wound-healing effect of derivatives of imidazole (IEM-1181) was evaluated as a 10% ointment on models of aseptic full-layer linear and planar skin wounds. Results. It was found that the compound IEM-1181 in the form of 10% ointment has a pronounced on skin wound healing effect, manifested in the qualitative features of the regenerative healing process. With local application of the ointment containing the tested compound, the strength of the tissue formed on the site of a full-layer linear skin wound was increased when its edges were stretched, and the healing time of full-layer planar wounds was reduced. The morphological picture of the tissue formed at the site of the wound defect corresponded to complete healing by primary tension with complete epithelization of the surface and squamous cell differentiation of the epithelial regenerate without signs of inflammation and scar formation. Conclusion. The results of the study on experimental models of full-layer linear and planar skin wounds indicate that the wound healing effect of the tested ointment is due to the anti-inflammatory activity of the IEM-1181 compound

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