Preparation of Small-Diameter Silk Fibroin Tubular Scaffolds with Electrospinning Method

2013 ◽  
Vol 745-746 ◽  
pp. 1-5
Author(s):  
Jing Xin Zhu ◽  
Yan Long Ma ◽  
Tetsuo Asakura
Keyword(s):  
Silk Fibroin ◽  
Fiber Diameter ◽  
Small Diameter ◽  
Electrospun Nanofibers ◽  
Random Coil ◽  
Diameter Distribution ◽  
Ethanol Treatment ◽  
Regenerated Silk Fibroin ◽  
Tubular Scaffold ◽  
Mandrel Diameter

In recent years, electrospinning has received much attention to prepare porous tubular scaffold due to electrospun nanofibers mat, which can mimic the structure characteristics of the extracellular matrix (ECM). In the present paper, silk fibroin (SF) tubular scaffolds with small-diameter were prepared by electrospinning from regenerated silk fibroin (RSF) aqueous solutions with rotating mandrel collector. The morphology and fiber diameter distribution of SF tubular scaffolds were influenced much by the concentration of regenerated silk fibroin (RSF) solution, collecting distance and mandrel diameter. The results showed that SF tubular scaffold, which was in flat surface with smaller fiber diameter and uniform distributed, could be obtained at the concentration of 22%, collection distance of 10 cm and mandrel diameter of 4 mm. The structure of SF tubular scaffolds before and after ethanol treatment was characterized by FTIR and XRD. After ethanol treatment, the SF tubular scaffolds had a conformation transition from random-coil conformation to Silk II conformation.

Effect of Solvent on the Characteristics of Electrospun Regenerated Silk Fibroin Nanofibers

2007 ◽  
Vol 342-343 ◽  
pp. 813-816 ◽  
Author(s):  
Lim Jeong ◽  
Kuen Yong Lee ◽  
Won Ho Park
Keyword(s):  
Secondary Structure ◽  
Formic Acid ◽  
Silk Fibroin ◽  
Structural Changes ◽  
Effective Means ◽  
Electrospun Nanofibers ◽  
Random Coil ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
Β Sheet

Nonwoven nanofiber matrices were prepared by electrospinning a solution of silk fibroin (SF) dissolved either in formic acid or in 1,1,1,3,3,3-hexafluoro-2-isopropyl alcohol (HFIP). The mean diameter of the electrospun nanofibers prepared from SF dissolved in formic acid was 80 nm with a unimodal size distribution, which was smaller than those prepared from HFIP (380 nm). SF nanofibers were then treated with an aqueous methanol solution, and structural changes due to solvent-induced crystallization of SF were investigated using IR and 13C solid-state CP/MAS NMR spectroscopy. SF nanofibers prepared from formic acid were found to have a higher proportion of β-sheet conformations than those prepared from HFIP. Methanol treatment provided a fast and effective means to alter the secondary structure of both types of SF nanofibers from a random coil form to a β-sheet form. As demonstrated in the present study, this approach to controlling the dimensions and secondary structure of proteins using various solvents may be useful for the design and tailoring of materials for biomedical applications, especially for tissue engineering applications.

Electrospinning of Regenerated Silk Fibroin/Polybutylene Terephthalate Blended Mats and their Surface Wettability

2012 ◽  
Vol 531-532 ◽  
pp. 531-534
Author(s):  
Yun Qian Cao ◽  
Qin Fei Ke ◽  
Xiang Yu Jin ◽  
Sha Sha Guo
Keyword(s):  
Silk Fibroin ◽  
Fiber Diameter ◽  
Contact Angles ◽  
Surface Wettability ◽  
Electrospun Fibers ◽  
Average Fiber Diameter ◽  
Polybutylene Terephthalate ◽  
Regenerated Silk Fibroin ◽  
Electrospinning Method ◽  
Nanofiber Membranes

In this paper, regenerated silk fibroin/polybutylene terephthalate blended mats were prepared using electrospinning method with different blending ratios. The influence of regenerated silk fibroin/polybutylene terephthalate ratio on the morphology behaviors, fiber diameter and the surface wettability of the blended mats were studied. The morphology of the electrospun fibers were characterized by SEM. The average fiber diameter and its distribution can be obtained from the SEM pictures using software Image J. The average fiber diameter was 280nm to 486nm and it changed with the blending ratio. The contact angles and penetration times were used to characterize the surface wettability of the nanofiber membranes. It was found that with the increase of regenerated silk fibroin amount, the surface contact angles and penetration times decreased, which meant that the wettability was greatly improved.

A comparison of centrifugally-spun and electrospun regenerated silk fibroin nanofiber structures and properties

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98553-98558 ◽  
Author(s):  
Chen Liu ◽  
Jiaqi Sun ◽  
Min Shao ◽  
Bin Yang
Keyword(s):  
Thermal Properties ◽  
Silk Fibroin ◽  
Secondary Structures ◽  
Random Coil ◽  
Structures And Properties ◽  
Regenerated Silk Fibroin ◽  
Centrifugal Spinning ◽  
Β Sheet

Centrifugal spinning converts the conformation of silk fibroin from random coil to β-sheet more easily than electrospinning, which results in fiber differences on secondary structures, orientation and thermal properties.

scholarly journals Fabrication of aqueous-based dual drug loaded silk fibroin electrospun nanofibers embedded with curcumin-loaded RSF nanospheres for drugs controlled release

RSC Advances ◽  
2017 ◽  
Vol 7 (89) ◽  
pp. 56550-56558 ◽  
Author(s):  
Huijun Li ◽  
Jingxin Zhu ◽  
Song Chen ◽  
Lan Jia ◽  
Yanlong Ma
Keyword(s):  
Controlled Release ◽  
Silk Fibroin ◽  
Electrospun Nanofibers ◽  
Electrospinning Technique ◽  
Regenerated Silk Fibroin ◽  
Dual Drug

This paper presents a new nanofabrication method for dual drug loaded regenerated silk fibroin (RSF) nanofibers, based on a simple, colloid-electrospinning technique.

scholarly journals Synthesis of Electrospun Nanofibers Membrane and Its Optimization for Aerosol Filter Application

2016 ◽  
Vol 1 ◽  
Author(s):  
Abdul Rajak
Keyword(s):  
Pressure Drop ◽  
Fiber Diameter ◽  
Polymer Concentration ◽  
Electrospun Nanofibers ◽  
Diameter Distribution ◽  
Air Filtration ◽  
Sem Image ◽  
Aerosol Filtration ◽  
Electrospinning Method ◽  
Fiber Diameter Distribution

Nanofibers membranes were synthesized using electrospinning method for air filtration application. Polyacrylonitrile (PAN) with three different concentrations as the polymeric matrix of the nanofibers membrane is used. In the aerosol filtration, the pressure drop is one of the most important parameters, which is determined by the membrane characteristics. One of the parameters that influence the characteristics of membrane is concentration of polymer solution, in which it will determine the diameter of fiber. In this study, the relation between the PAN concentration and the pressure drop in air filtration test was examined. Three different concentrations of PAN solution (6, 9, and 12 wt.%) were employed under the same process parameters of electrospinning. The fiber diameter distribution of each membrane was measured from its scanning electron microscope (SEM) image. The three concentrations resulted in significant different effect to the pressure drop that proved the existing correlation between the polymer concentration and the air pressure drop.

Mechanical and Thermal Characteristics of Optimized Electrospun Nylon 6,6 Nanofibers by Using Taguchi Method

NANO ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. 1950139
Author(s):  
Saleh S. Abdelhady ◽  
Said H. Zoalfakar ◽  
M. A. Agwa ◽  
Ashraf A. Ali
Keyword(s):  
Fiber Diameter ◽  
Thermal Characteristics ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Statistical Technique ◽  
Electrospinning Process ◽  
Diameter Distribution ◽  
Solvent Ratio ◽  
Average Fiber Diameter ◽  
Nylon 6,6

This study is an attempt to optimize the electrospinning process to produce minimum Nylon 6,6 nanofibers by using Taguchi statistical technique. Nylon 6,6 solutions were prepared in a mixture of formic acid (FA) and Dichloromethane (DCM). Design of experiment by using Taguchi statistical technique was applied to determine the most important processing parameters influence on average fiber diameter of Nylon 6,6 nanofiber produced by electrospinning process. The effects of solvent/nylon and FA/DCM ratio on average fiber diameter were investigated. Optimal electrospinning conditions were determined by using the signal-to-noise (S/N) ratio that was calculated from the electrospun Nylon 6,6 nanofibers diameters according to “the-smaller-the-better” approach. The optimum Nylon 6,6 concentration (NY%) and FA/DCM ratio were determined. The morphology of electrospun nanofibers is significantly altered by FA/DCM solvent ratio as well as Nylon 6,6 concentration. The smallest diameter and the narrowest diameter distribution of Nylon 6,6 nanofibers ([Formula: see text][Formula: see text]nm) were obtained for 10 wt% Nylon 6,6 solution in 80 wt% FA and 20 wt% DCM. An increase of 118%, 280% and 26% in tensile strength, modulus of elasticity and elongation at break over as-cast was obtained, respectively. Glass transition temperature of Nylon 6,6 nanofibers were determined by using differential scanning calorimeter (DSC). Analysis of variance ANOVA shows that NY% is the most influential parameter.

EFFECT OF STORAGE TIME AND CONCENTRATION ON STRUCTURE OF REGENERATED SILK FIBROIN SOLUTION

2006 ◽  
Vol 20 (25n27) ◽  
pp. 3878-3883 ◽  
Author(s):  
FANG XIE ◽  
HUILI SHAO ◽  
XUECHAO HU
Keyword(s):  
Aqueous Solution ◽  
Silk Fibroin ◽  
Storage Time ◽  
Conformational Transition ◽  
Solution Concentration ◽  
Cd Spectroscopy ◽  
Random Coil ◽  
Conformational Transformation ◽  
Regenerated Silk Fibroin ◽  
Α Helix

Concentrated regenerated silk fibroin (RSF) aqueous solutions with concentration close to that of the native silk fibroin (15.5%, 25.5% and 31%) were prepared. The effect of storage time and concentration on the conformational transition of the concentrated RSF aqueous solution was studied by Raman spectroscopy and circular dichroism (CD) spectroscopy. At the same time, the conformational change of RSF aqueous solution in flowing state was also investigated. It was found that the conformation of silk fibroin was changed gradually from random coil/α-helix to β-sheet structure during the storage. And the conformational transformation was accelerated with the increasing of the RSF aqueous solution concentration. When the solution was in flowing state, the conformational transformation was also accelerated.

Self-assembly of regenerated silk fibroin from random coil nanostructures to antiparallel β-sheet nanostructures

Biopolymers ◽  
2014 ◽  
Vol 101 (12) ◽  
pp. 1181-1192 ◽  
Author(s):  
Jian Zhong ◽  
Mengjia Ma ◽  
Wenying Li ◽  
Juan Zhou ◽  
Zhiqiang Yan ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Self Assembly ◽  
Random Coil ◽  
Regenerated Silk Fibroin ◽  
Β Sheet

scholarly journals The Relationship between Secondary Structure and Biodegradation Behavior of Silk Fibroin Scaffolds

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Yongpei Hu ◽  
Qin Zhang ◽  
Renchuan You ◽  
Lingshuang Wang ◽  
Mingzhong Li
Keyword(s):  
Tissue Engineering ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Degradation Rate ◽  
Mechanical Performance ◽  
Tissue Growth ◽  
Random Coil ◽  
Regenerated Silk Fibroin ◽  
Sheet Structure ◽  
The Relationship

Silk fibroin has a unique and useful combination of properties, including good biocompatibility and excellent mechanical performance. These features provided early clues to the utility of regenerated silk fibroin as a scaffold/matrix for tissue engineering. The silk fibroin scaffolds used for tissue engineering should degrade at a rate that matches the tissue growth rate. The relationship between secondary structure and biodegradation behavior of silk fibroin scaffolds was investigated in this study. Scaffolds with different secondary structure were prepared by controlling the freezing temperature and by treatment with carbodiimide or ethanol. The quantitative proportions of each secondary structure were obtained by Fourier transform infrared spectroscopy (FTIR), and each sample was then degradedin vitrowith collagenase IA for 18 days. The results show that a high content ofβ-sheet structure leads to a low degradation rate. The random coil region in the silk fibroin material is degraded, whereas the crystal region remains stable and the amount ofβ-sheet structure increases during incubation. The results demonstrate that it is possible to control the degradation rate of a silk fibroin scaffold by controlling the content ofβ-sheet structure.

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