Antibacterial Properties Evaluation of a Novel Polyurethane-Based Root Canal Sealer

2014 ◽  
Vol 804 ◽  
pp. 231-234
Author(s):  
Jian Wang ◽  
Ming Hui Zhao ◽  
Yu Bao Li ◽  
Yi Zuo ◽  
Bin Sun ◽  
...  
Keyword(s):  
Root Canal ◽  
Direct Contact ◽  
Antibacterial Properties ◽  
Adhesion Assay ◽  
Root Canal Sealer ◽  
Contact Test ◽  
Silver Phosphate ◽  
Application Potential ◽  
Rate Test ◽  
Direct Contact Test

Elimination of microorganisms from the root canal system and the prevention of subsequent reinfection are of importance for long-term endodontic treatment. The application of a sealer with antibacterial properties may reduce the reinfection and improve the success rate of the root filling treatment. The aim of this paper is to evaluate the antibacterial properties of a novel root canal sealer based on injectable self-curing polyurethane with silver phosphate (PU/Ag3PO4). The antibacterial abilities were assessed by direct contact test, anti-bacterial adhesion assay and bacteriostatic rate test. The results show that the fabricated PU/Ag3PO4sealer can completely inhibit the bacterial growth and prevent bacterial adhension effectively. The bacteriostatic rate is 93.6% and 98.1% when the bacteria incubated with PU/Ag3PO4 sealer for 7 and 24h respectively. The strong antibacterial abilities suggest that PU/Ag3PO4 sealer has great application potential in the field of root canal filling.Key words: Root canal sealer, polyurethane, silver phosphate, antibacterial properties, direct contact test

scholarly journals Evaluation of antibacterial effects of pulp capping agents with direct contact test method

2014 ◽  
Vol 08 (01) ◽  
pp. 095-099 ◽  
Author(s):  
Muhammet Yalcin ◽  
Ugur Arslan ◽  
Ayse Dundar
Keyword(s):  
Direct Contact ◽  
Antibacterial Properties ◽  
Test Method ◽  
Control Group ◽  
Capping Agents ◽  
Pulp Capping ◽  
Contact Test ◽  
Microtiter Plates ◽  
Level Of Significance ◽  
Direct Contact Test

ABSTRACT Objectives: Calcium hydroxide has been used in dentistry as a major capping material having the capacity to introduce the formation of a mineralized dentin bridge, but it has no direct inducing effect to the pulp cells. The purpose of this study was to evaluate the antibacterial properties of three different pulp capping agents using a direct contact test (DCT). Materials and Methods: The antibacterial properties of three pulp capping agents were evaluated a DCT. For the DCT, wells (n = 12) of 96-microtiter plates were coated with the tested cements (Dycal, Dentsply, USA; DiaRoot BioAggregate, Diadent, Holland; Calcimol LC, Voco, Germany) and Kalzinol (zinc oxide/eugenol cement, Dentsply, USA) was used as control material. A Lactobacillus casei suspension was placed on the surface of each specimen for 1 h at 37°C. Bacterial growth was monitored for 16 h with a temperature-controlled microplate spectrophotometer. The kinetics of the outgrowth in each well were recorded continuously at 650 nm every 30 min. The data were analyzed by one-way ANOVA, and Tamhane's T2 multiple comparison test. The level of significance was determined as P < 0.05. Results: All pulp capping agents showed an increase in the logarithmic growth rate of L. casei when compared with the control group (P < 0.05). Therefore, all pulp capping agents did not show antibacterial activity. Conclusions: The tested pulp capping agents haven't got antibacterial properties. Therefore, they should be used carefully when pulp is exposed or only very thin dentin remained over the pulp to avoid bacterial contamination.

Antibacterial investigation of titanium-copper alloys using luminescent Staphylococcus epidermidis in a direct contact test

2019 ◽  
Vol 97 ◽  
pp. 707-714 ◽  
Author(s):  
Lee Fowler ◽  
Oscar Janson ◽  
Håkan Engqvist ◽  
Susanne Norgren ◽  
Caroline Öhman-Mägi
Keyword(s):  
Staphylococcus Epidermidis ◽  
Direct Contact ◽  
Copper Alloys ◽  
Contact Test ◽  
Direct Contact Test

Direct Contact Test for Evaluating Bacterial Growth on Machined and Rough Surface Implants

2017 ◽  
Vol 26 (6) ◽  
pp. 899-903 ◽  
Author(s):  
Shimshon Slutzkey ◽  
Ofer Moses ◽  
Haim Tal ◽  
Avi Meirowitz ◽  
Shlomo Matalon
Keyword(s):  
Rough Surface ◽  
Bacterial Growth ◽  
Direct Contact ◽  
Contact Test ◽  
Direct Contact Test

scholarly journals Ecotoxicity testing of airborne particulate matter—comparison of sample preparation techniques for the Vibrio fischeri assay

2021 ◽  
Author(s):  
Nora Kováts ◽  
Katalin Hubai ◽  
Tsend-Ayush Sainnokhoi ◽  
András Hoffer ◽  
Gábor Teke
Keyword(s):  
Particulate Matter ◽  
Organic Solvents ◽  
Direct Contact ◽  
Vibrio Fischeri ◽  
Water Extract ◽  
Airborne Particulate Matter ◽  
Airborne Particulate ◽  
Contact Test ◽  
Extraction Procedures ◽  
Direct Contact Test

AbstractThe bioassay based on the bioluminescence inhibition of the marine bacterium Vibrio fischeri has been the most widely used test for the assessment of airborne particulate matter ecotoxicity. Most studies available use an extract of the solid sample, either made with water or organic solvents. As an alternative, a whole-aerosol test is also available where test bacteria are in actual contact with contaminated particles. In our study, different extraction procedures were compared to this direct contact test based on the V. fischeri assay and analytical measurements. The lowest PAH content and the highest EC50 were determined in water extract, while the highest PAH amount and lowest EC50 were measured in dichloromethane, hexane, and dimethyl-sulphoxide extracts. EC50 of the direct contact test was comparable to that of the methanol extract. Our results suggest that the sensitivity of the direct contact test equals to that of extraction procedures using organic solvents, moreover, it is mimicking an environmentally realistic exposure route.

scholarly journals Antimicrobial and cytotoxic capacity of pyroligneous extracts films of Eucalyptus grandis and chitosan for oral applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Juliana Leitzke Santos de Souza ◽  
Tomaz Alves ◽  
Laísa Camerini ◽  
Fernanda Nedel ◽  
Angela Diniz Campos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Direct Contact ◽  
Eucalyptus Grandis ◽  
Effective Control ◽  
Natural Polymers ◽  
Contact Test ◽  
Enamel Demineralization ◽  
Chitosan Films ◽  
Direct Contact Test

AbstractChitosan films containing distilled pyroligneous extracts of Eucalyptus grandis (DPEC), characterized and developed by Brazilian Agricultural Research Corporation—Embrapa Temperate Agriculture (EMBRAPA-CPACT), were evaluated for antimicrobial activity against Candida albicans, Streptococcus mutans, and Lactobacillus acidophilus by direct contact test. Further, their capacity for the prevention of teeth enamel demineralization and cytotoxicity in vitro were also determined. The natural polymers were tested at different concentrations (1500–7500 µg mL−1) and the formulation of an experimental fluoride varnish with antimicrobial activity was evaluated by direct contact test, whereas cytotoxicity was analyzed through the colorimetric MTT assay. Preliminary data showed no statistically significant differences in cytotoxicity to NIH/3T3 cell line when DPEC is compared to the control group. On the other hand, the antimicrobial capacity and demineralization effects were found between the test groups at the different concentrations tested. Chitosan films containing distilled pyroligneous extracts of E. grandis may be an effective control strategy to prevent biofilm formation related to dental caries when applied as a protective varnish. They may inhibit the colonization of oral microorganisms and possibly control dental caries through a decrease in pH and impairment of enamel demineralization.

scholarly journals An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Lavanya Anumula ◽  
Swaroop Kumar ◽  
Venkata Suneel Kumar ◽  
Chandra Sekhar ◽  
Murali Krishna ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Glass Ionomer ◽  
Polydimethyl Siloxane ◽  
Contact Test ◽  
Urethane Dimethacrylate ◽  
Endodontic Sealers ◽  
Direct Contact Test

Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based sealer was the most effective and urethane dimethacrylate resin-based sealer was the least effective against Enterococcus faecalis, whereas glass-ionomer-based and polydimethyl-siloxane-based sealers were effective only for a short period. Inhibition of the bacterial growth is related to the direct contact of the microorganism with the sealer.

scholarly journals Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts

2017 ◽  
Vol 11 (02) ◽  
pp. 141-148 ◽  
Author(s):  
Sandra Chakar ◽  
Sylvie Changotade ◽  
Nada Osta ◽  
Issam Khalil
Keyword(s):  
Cell Viability ◽  
Root Canal ◽  
Direct Contact ◽  
Human Fibroblasts ◽  
Qualitative Evaluation ◽  
Root Canal Sealer ◽  
Indirect Contact ◽  
Significance Level ◽  
Diphenyltetrazolium Bromide ◽  
Significant Difference

ABSTRACT Objectives: The purpose of this study was to evaluate the cytotoxicity of a new bioceramic-based root canal sealer (BioMM) by direct and indirect contact with human fibroblasts and to compare it with a zinc oxide-eugenol sealer, the Pulp Canal Sealer-extended working time (PCS-EWT). Materials and Methods: Cell viability was assessed through direct and indirect contact between human fibroblasts and sealer. Direct contact was performed at 24 h, whereas the indirect contact was performed at 24 and 48 h at different concentrations: 100%, 50%, and 25%. After direct contact, 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used and the optical density was measured by a spectrophotometer. Giemsa stain was also performed for a qualitative evaluation of the cells.Statistical Analysis Used: Shapiro–Wilk test was used to verify the normality of distribution of the variable. Data were analyzed by analysis of variance and Tukey tests using SPSS for Windows software 18.0. The significance level used was P < 0.05.Results: Direct contact showed a significantly higher cell viability with BioMM as compared to PCS-EWT (P = 0.002). Cell viability at 24 h was significantly higher with BioMM compared to PCS-EWT for the concentrations of 50% (P = 0.004) and 25% (P = 0.003), whereas no significant difference was noted at 100% (P = 0.141). Cell viability at 48 h was significantly higher with BioMM as compared to PCS-EWT at 25% (P = 0.007). No significant difference was observed at 100% (P = 0.484) and 50% (P = 0.185).Conclusion: BioMM may be considered minimally cytotoxic if accidentally extruded into the periapical tissues.

scholarly journals Antibacterial and Antibiofilm Effect of Low Viscosity Chitosan againstStaphylococcus epidermidis

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Inger Sofie Dragland ◽  
Håkon Valen Rukke ◽  
Ida S. R. Stenhagen ◽  
Jessica Lönn-Stensrud ◽  
Hilde M. Kopperud
Keyword(s):  
Biofilm Formation ◽  
Direct Contact ◽  
Bactericidal Effect ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Contact Test ◽  
Planktonic Growth ◽  
Low Viscosity ◽  
New Treatment ◽  
Direct Contact Test

Aim. The aim of this study was to investigate the antibacterial and antibiofilm properties of low viscosity chitosan onS. epidermidisgrowth and biofilm formation.Methods and Results. The antibacterial and antibiofilm properties were investigated, during both planktonic growth and biofilm formation. This was performed using different concentrations in media and by coating on polystyrene surfaces. In addition, the bactericidal effect was investigated using a modified direct contact test. The results showed that low viscosity chitosan in media had both a bacteriostatic and bactericidal effect on planktonic growth and biofilm formation ofS. epidermidisin a concentration dependent manner. Polystyrene discs coated with chitosan reduced both early biofilm formation (6 h) and late biofilm formation (18 h), as confirmed by scanning electron microscopy. The modified direct contact test showed a bactericidal effect.Conclusion. This study demonstrated that low viscosity chitosan has a bacteriostatic and bactericidal activity againstS. epidermidisand that the activity is dependent on the amount of chitosan added. In addition, low viscosity chitosan reduced biofilm formation both when added to media and when coated on polystyrene surfaces.Significance and Impact of Study. Low viscosity chitosan could be a contribution to new treatment approaches of biofilm-related infections ofS. epidermidis.

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