Preliminary Study on Micropropagation of Hylocereus polyrhizus with Waste Coconut Water and Sucrose

2020 ◽  
Vol 981 ◽  
pp. 316-321
Author(s):  
Zhe Cheng Ng ◽  
Suat Hian Tan ◽  
Siti Hamidah Radiyah Shiekh Mahmud ◽  
Nyuk Ling Ma
Keyword(s):  
Growth And Development ◽  
Fruit Tree ◽  
Coconut Water ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Dragon Fruit ◽  
Suitable Amount ◽  
Preliminary Study ◽  
Hylocereus Polyrhizus

Mature coconut water is always been thrown away as a waste. However, it contain a lots of nutritional elements such as amino acids, pytohormones and minerals. Plant needs some essential nutrients besides pytohormones for their growth and development. In this study, the effect of waste mature coconut water and sucrose was observed on the micropropagation of dragon fruit tree (Hylocereus polyrhizus). The stem was inoculated on MS medium containing Benzyleaminopurine (BAP) 0.03mg/L BAP and 0.01mg/L Naphthaleneacetic acid (NAA) supplemented with waste mature coconut water in various concentrations (0%, 2%, 4% and 6% v/v) and with various concentrations : 0%, 1%, 2% and 3% of sucrose respectively. As the concentration of the waste mature coconut water and sucrose increased up to 4 % and 3%, increase in the elongation of the stem and the number of root regenerated per explant was observed repectively. In conclusion, waste mature coconut water has enhanced the elongation of the shoots but has not promoted on the root induction with the suitable amount of sucrose.

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

scholarly journals Establishment of a Rapid and Efficient Micropropagation System for Succulent Plant Haworthia turgida Haw.

HortScience ◽  
2017 ◽  
Vol 52 (9) ◽  
pp. 1278-1282 ◽  
Author(s):  
Boling Liu ◽  
Hongzhou Fang ◽  
Chaorong Meng ◽  
Ming Chen ◽  
Qingdong Chai ◽  
...  
Keyword(s):  
Callus Induction ◽  
Adventitious Shoots ◽  
Germplasm Conservation ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Induction Medium ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Succulent Plant

In the present study, the effect of plant growth regulators (PGRs) on callus regeneration, adventitious shoot differentiation, and root formation of Haworthia turgida Haw. was investigated. The greatest callus induction percentage (95.6%) was achieved with leaf explants inoculated on Murashige and Skoog (MS) medium with 1.0 mg·L−1 6-benzyladenine (BA) and 0.1 mg·L−1 1-naphthaleneacetic acid (NAA), and this callus induction medium supplemented with 2.5 mg·L−1 thidiazuron (TDZ) was optimal for callus proliferation. The maximum number of shoots (25.7) was obtained when the callus was cultured on MS medium supplemented with 1.0 mg·L−1 BA and 0.2 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest number of roots per shoot (6.2) and highest rooting frequency (82.0%) were obtained when adventitious shoots were inoculated on MS medium with 0.05 mg·L−1 NAA. Regenerated plantlets were transferred to a mixture of vermiculite and soil and acclimated in a greenhouse. The survival rate of the transplanted plantlets was about 91.6%. The rate of ex vitro rooting was 83.3%, indicating that this technique is effective for root induction in H. turgida. This study has established a rapid and efficient micropropagation system that can be beneficial for commercial cultivation and germplasm conservation of H. turgida.

scholarly journals Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

2019 ◽  
Vol 20 (9) ◽  
pp. 2141 ◽  
Author(s):  
Han Yong Park ◽  
Doo Hwan Kim ◽  
Ramesh Kumar Saini ◽  
Judy Gopal ◽  
Young-Soo Keum ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Large Scale ◽  
Shoot Tip ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Leaf Tissues

The goal of this study was to establish an efficient protocol for the large-scale propagation of Mertensia maritima (L.) Gray, and evaluate the carotenoid, fatty acid, and tocopherol contents in the leaves of in vitro regenerated shoots. Surface-disinfected node and shoot tip explants were placed on semisolid Murashige and Skoog (MS) medium with 0–16 µM N6-benzyladenine (BA), kinetin, (KN), and thidiazuron (TDZ) alone, or in combination with, 1 or 2 µM α-naphthaleneacetic acid (NAA). Of the three different cytokinins employed, TDZ elicited the best results for axillary shoot proliferation. A maximum frequency of shoot initiation above 84%, with a mean of 8.9 and 4.8 shoots per node and shoot tip, respectively, was achieved on the culture medium supplemented with 4 µM TDZ. A combination of TDZ + NAA significantly increased the percentage of multiple shoot formation and number of shoots per explant. The best shoot induction response occurred on MS medium with 4 µM TDZ and 1 µM NAA. On this medium, the node (93.8%) and shoot tip (95.9%) explants produced an average of 17.7 and 8.6 shoots, respectively. The highest root induction frequency (97.4%) and number of roots per shoot (25.4), as well as the greatest root length (4.2 cm), were obtained on half-strength MS medium supplemented with 4 µM indole-3-butyric acid (IBA). The presence of six carotenoids and α-tocopherol in the leaf tissues of M. maritima was confirmed by HPLC. Gas chromatography-mass spectrometry analysis confirmed the presence of 10 fatty acids, including γ-linolenic acid and stearidonic acid in the leaf tissues of M. maritima. All-E-lutein (18.49 μg g−1 fresh weight, FW), α-tocopherol (3.82 μg g−1 FW) and α-linolenic acid (30.37%) were found to be the significant compounds in M. maritima. For the first time, a successful protocol has been established for the mass propagation of M. maritima with promising prospects for harnessing its bioactive reserves.

Pectin Extraction and Characterization from Red Dragon Fruit (Hylocereus polyrhizus): A Preliminary Study

2010 ◽  
Vol 10 (7) ◽  
pp. 631-636 ◽  
Author(s):  
K.K. Woo ◽  
Y.Y. Chong ◽  
S.K. Li Hiong ◽  
P.Y. Tang
Keyword(s):  
Dragon Fruit ◽  
Preliminary Study ◽  
Hylocereus Polyrhizus

Micropropagation of two threatened Tasmanian species of Calocephalus (Asteraceae), with comments on phenotypic plasticity

2003 ◽  
Vol 51 (4) ◽  
pp. 415 ◽  
Author(s):  
Rebecca Jane Sands ◽  
Natalie Ruth Brown ◽  
Anthony Koutoulis
Keyword(s):  
Phenotypic Plasticity ◽  
Plant Growth Regulator ◽  
Root Formation ◽  
Indoleacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Ex Vitro ◽  
Morphological Differences

Micropropagation systems were developed for Calocephalus citreus Less. and C. lacteus Less., two threatened Tasmanian members of the Asteraceae. Disinfected cold-treated capitula were used to initiate regeneration. For C. citreus, initiation was achieved on Murashige and Skoog (MS) medium with 0.1�mg�L–1 or 0.5�mg�L–1 indoleacetic acid (IAA) and 1�mg�L–1 6-benzylaminopurine (BAP) in 5�weeks, while for C. lacteus initiation was achieved on MS with α-naphthaleneacetic acid (NAA) (0.1�mg�L–1) in 3�weeks and on MS without any plant growth regulator (PGR) in 6�weeks. Multiplication was achieved in both species on MS with various concentrations of IAA (0.01–0.5�mg�L–1) and BAP (0.1–1�mg�L–1). In C. citreus, shooting in all treatments did not differ significantly from PGR-free MS, while in C. lacteus PGR-free MS was one of the better treatments. Multiplication media also initiated root formation in C. lacteus, thereby facilitating immediate planting out. Optimal root induction in C. citreus was achieved by using MS with 1�g�L–1 activated charcoal. Clear morphological differences between in vitro and ex vitro plants of both species were observed. This phenotypic plasticity was more pronounced in C. lacteus than in C. citreus. As C. lacteus has a wider distribution than C. citreus and C. lacteus was more responsive during many stages of the micropropagation process, it may be possible to use the culture-induced phenotype to provide insights into the ecology of plant species.

scholarly journals Callus Induction through Anther and Ovary of Kenaf (Hibiscus cannabinus L.)

2015 ◽  
Vol 3 (1) ◽  
pp. 6-13
Author(s):  
Ahmed Mahmood Ibrahim ◽  
Fatimah Kayat ◽  
DWI Susanto ◽  
Mohammed Ariffulah ◽  
Pedram Kashiani
Keyword(s):  
Culture Condition ◽  
Callus Induction ◽  
Cold Treatment ◽  
Treatment Plant ◽  
Naphthaleneacetic Acid ◽  
Flower Initiation ◽  
Root Induction ◽  
Ms Medium ◽  
Flower Buds ◽  
Induction Frequency

In this research new protocol has been developed that would induce callus from anther and ovary of Kenaf. The haploid technique is a fast and efficient tool for developing new varieties in a comparatively short time. The present study was carried out to investigate the effect of cold treatment, plant growth regulators (PGR) and culture condition on callus induction from Kenaf anther and ovary culture. Kenaf HF992 cultivar was chosen as an explants material, several trails were carried out to investigate the Androgenesis and gynogenesis ability before we succeed to get high percentage of callus. Flower buds at the appropriate stage of anther and ovary development were sterilized and the anthers and ovary were carefully excised from the flowers and underwent to various pretreatments and inoculated into media contained different combinations of PGR like NAA(a-naphthaleneacetic acid), BAP(N6-benzyladenine), 2iP(N6-(2-Isopentenyl) adenine) and TDZ (Thidiazuron)and kept in the dark place for different periods before transferred to light conditions. The best callus induction frequency from anther was 90.00 % in the optimized MS medium fortified with 3.0 mg/l 2iP + 3.0 mg/l NAA at the stage the microspores were at the uninucleate stage and the anther was about 7±1 mm long collected. And the best callus induction frequency from ovary was 91.25% in the optimized semi solid MS medium fortified with 3.0 mg/l 2iP + 3.0 mg/l NAA, and the flower buds was about 24±1 mm length which was collected 3-5 weeks after flower initiation. The effect of culture condition was highly significant, the root induction was highest (83.75% & 87.50%) of anther and ovary respectively when it kept in dark place for 28 days.

scholarly journals Effect of Coconut Water on Root Induction of Musa (AA Group) ‘KLUAI NAM THAI’ In Vitro

2021 ◽  
Vol 58 (1) ◽  
pp. 1640-1643
Author(s):  
Thanwamas Kassanuk Et al.
Keyword(s):  
Environmental Changes ◽  
Culture Technique ◽  
The Other ◽  
Coconut Water ◽  
Root Induction ◽  
Ms Medium ◽  
Randomized Design ◽  
Risk Of Extinction ◽  
Completely Randomized Design

Kluai Nam Thai’ (Musa AA group) are rare in Thailand and it is at risk of extinction because of environmental changes and human behavior. Hence, the plant tissue culture technique is used for propagation. This research aimed to study the effect of coconut water on root induction of Kluai Nam Thai in vitro. The shootlets from laboratory were cultured on Murashige and Skoog (MS) medium supplemented with coconut water at concentrations 10, 15, 20, and 25% for 1 month. The experimental design was Completely Randomized Design (CRD). The results indicated that MS medium supplemented with 20% coconut water gave the highest average number of roots at 1.85 while MS medium supplemented with 10% coconut water gave the highest length of roots at 2.34 cm which were significantly different (p<0.01) from the other concentrations

scholarly journals Establishment of Cell Suspension Culture and Plant Regeneration in Abrus precatorius L., a Rare Medicinal Plant

2012 ◽  
Vol 4 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Mohammad Serajur RAHMAN ◽  
Mohammad Abdul Bari MIAH ◽  
Mohammad Shahadat HOSSAIN ◽  
Ahmad Humayan KABIR ◽  
Mohammad Motiur RAHMAN
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Liquid Media ◽  
Indolebutyric Acid ◽  
Abrus Precatorius ◽  
Isolated Cell

A new protocol has been developed for cell culture and in vitro regeneration of Abrus precatorius that holds enormous potentiality for preparation of medicines. In vitro grown calli were cultured in Murashige and Skoog (MS) liquid media in agitated condition fortified with 0.5 mg/l 6-Benzylaminopurine. Growth curve of cells revealed that the cells continued to grow until 12 days of culture and got the highest peak from day 6-8. Isolated cell was found to produce highest 8.2% calli when suspended on MS medium supplemented with 0.5 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. Callus derived from single cell produced highest number of embryo (25-28%) cultured on MS medium fortified with 2.0 mg/l 6-Benzylaminopurine and 0.2 mg/l 1-Naphthaleneacetic acid. The bipolar embryos were selected and optimum shoot formation was recorded on MS medium supplemented with 2.0 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. The optimum root induction was noticed in MS medium supplemented with 1.0 mg/l 3-Indolebutyric acid. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.

scholarly journals Micropropagation of Epilobium canum garretti (Firechalice) by Axillary Shoot Culture

HortScience ◽  
2018 ◽  
Vol 53 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Areej A. Alosaimi ◽  
Robert R. Tripepi ◽  
Stephen L. Love
Keyword(s):  
Shoot Culture ◽  
Shoot Proliferation ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Axillary Shoots ◽  
Shoot Height ◽  
Epilobium Canum

Epilobium canum subsp. garrettii (firechalice) is an herbaceous wildflower with landscape potential, but its seeds are difficult to germinate because of dormancy requirements. The objective of this study was to develop a complete micropropagation procedure for a selected accession of firechalice. Single-node stem explants from the plant were examined for their ability to establish on Murashige and Skoog (MS) medium or Woody Plant Medium (WPM). Shoot explants on MS medium supplemented with 4.4 μm benzyladenine (BA) produced more than double the number of axillary shoots compared to explants on WPM (12.6 vs. 4.9 shoots, P = 0.0001). Benzyladenine, kinetin (kin), 6-(γ,γ-dimethlyallylamino)purine (2iP), thidiazuron (TDZ), and meta-topolin (mT) at concentrations of 0, 1.1, 2.2, 4.4, or 8.8 μm were evaluated for shoot proliferation efficacy. Stem explants treated with 8.8 μm of BA or mT produced the most shoots, 11 or 15, respectively. Benzyladenine, 2iP, and kin failed to affect shoot height even at the highest concentrations used, but 4.4 or 8.8 μm TDZ reduced shoot height to less than half of the heights of control shoots (3.1 vs. 1.2 cm, P = 0.0001). Firechalice shoots formed three to four roots easily without auxin added to the medium, but four to six roots formed per shoot when using up to 9 μm of indole-3-butyric acid (IBA). In contrast, 9 μm naphthaleneacetic acid (NAA) prevented root formation. When using 0–9 μm IBA for rooting, 82.5% of the rooted shoots survived transplanting. Based on these results, optimum micropropagation of firechalice may be achieved with shoots established on MS medium plus 4.4 µm BA, a concentration of 4.4 or 8.8 μm BA or mT used for shoot proliferation, and use of up to 6 µm IBA during root induction should result in >80% shoot survival after transplanting.

scholarly journals In vitro plant regeneration in Banana (Musa sp.) cv. sabri

2012 ◽  
Vol 47 (2) ◽  
pp. 143-146
Author(s):  
Amdadul Huq ◽  
Shahina Akter ◽  
Shahina Islam ◽  
Salim Khan
Keyword(s):  
Coconut Water ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Stem Cuttings ◽  
In Vitro Plant Regeneration ◽  
Musa Sp ◽  
Best Response ◽  
Micro Propagation

The establishment of a micro-propagation protocol for banana (Musa sp.) cv. Sabri, was carried out using meristematic stem cuttings explant. MS medium supplemented with BAP singly or in combination with auxin, IAA and coconut water was used for this purpose. Highest percentage of shoot regeneration (90%) and maximum number of shoots (10) per explant were observed when cultured on MS + 4.0 mg/1 BAP + 2.0 mg/1 IAA + 13% (v/v) coconut water. Best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/1 IBA. The regenerated healthy rooted plantlets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Through this method, complete micro-propagated plantlets were obtained within three months.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11444   Bangladesh J. Sci. Ind. Res. 47(2), 143-146, 2012  

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