scholarly journals El efecto de la disponibilidad de agua durante el crecimiento de Lycopersicon chilense sobre la capacidad de sus semillas para germinar a distintas temperaturas y concentraciones de manitol y NaCl

2002 ◽  
Vol 75 (4) ◽  
Author(s):  
CARLOS MALDONADO ◽  
EDGAR PUJADO ◽  
FRANCISCO A. SQUEO
Keyword(s):  
Lycopersicon Chilense

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

Highly heterogeneous families of Ty1/copia retrotransposons in the Lycopersicon chilense genome

Gene ◽  
1998 ◽  
Vol 222 (2) ◽  
pp. 223-228 ◽  
Author(s):  
Mónica Yañez ◽  
Isabel Verdugo ◽  
Mariana Rodrı́guez ◽  
Salomé Prat ◽  
Simón Ruiz-Lara
Keyword(s):  
Lycopersicon Chilense ◽  
Copia Retrotransposons

scholarly journals (260) Development of SCAR and CAPS Markers Linked to Tomato Begomovirus Resistance Genes Introgressed from Lycopersicon chilense

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1090A-1090 ◽  
Author(s):  
Yuanfu Ji ◽  
John W. Scott
Keyword(s):  
Resistance Genes ◽  
Rapd Markers ◽  
Fragment Length Polymorphism ◽  
Rflp Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Breeding Lines ◽  
Sequence Characterized Amplified Region ◽  
Lycopersicon Chilense ◽  
Caps Markers ◽  
Leaf Curl Virus

Resistance to begomoviruses tomato mottle virus (ToMoV) and tomato yellow leaf curl virus (TYLCV) has been introgressed to tomato (Lycopersicon esculentum) from L. chilense accessions LA 1932, LA 2779, and LA 1938. Resistance genes have been mapped to three regions on chromosome 6 using randomly amplified polymorphic DNA (RAPD) markers. We call these regions 1, 2, and 3. To facilitate breeding by marker assisted selection, advanced breeding lines with resistance from the above sources were assayed for the presence of RAPD markers to determine which were most tightly linked to begomovirus resistance. The best RAPD markers were then converted to sequence characterized amplified region (SCAR) markers or cleaved amplified polymorphic sequence (CAPS) markers. In addition, selected restriction fragment length polymorphism (RFLP) markers near the three regions were converted into CAPS markers, which were tested for association with the advanced breeding lines. Only LA 2779 derivatives have the L. chilense introgression in region 1, which is near the location of the Ty-1 gene and spans across CAPS markers 32.5Cla and TG118. Two region 1 RAPD markers UBC197 and UBC621 were converted co-dominant SCAR or CAPS markers, which were present in all 16 resistant breeding lines tested. Derivatives from all three accessions have introgressions in region 2. Further assays with more markers in this region are under way to determine the lengths and locations of the introgressions. No tightly linked RAPD markers have been found for the resistance gene from LA 1932 in region 3. RFLP and CAPS markers are being used to more precisely locate the region 3 gene.

scholarly journals Comparison of Drought-induced Chitinase Expression Between Wild and Cultivated Tomatoes

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 798E-798
Author(s):  
Long-Xi Yu ◽  
Ann. F. Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Gene Expression ◽  
Water Stress ◽  
Drought Tolerance ◽  
Enzyme Assay ◽  
Northern Blot Analysis ◽  
Chitinase Activity ◽  
Chitinase Gene ◽  
Lycopersicon Chilense ◽  
Drought Tolerant ◽  
And Control

We have carried out a comparative study on chitinase gene expression and enzyme activity in Lycopersicon chilense (a drought-tolerant wild tomato) and L. esculentum under water stress. Both enzyme assay and Northern blot analysis revealed that chitinase expression was differentially induced by drought among the different genotypes. Higher induction of chitinase was found in tolerant species compared to the sensitive one. Among genotypes examined, L. chilense LA2747 presented the highest level of the chitinase induction, while the lowest level was found in L. esculentum HR86. Leaves of drought-stressed plants showed the highest expression and roots showed the lowest, with stems being intermediate. Chitinase activity was detected in flowers of both drought-stressed and control plants. The measurement of leaf water potentials of different genotypes revealed a correlation between drought tolerance and the level of chitinase expression during water stress. Our results suggest that the chitinase might be involved in drought tolerance of L. chilense.

Negative regulation of gene expression of a novel proline-, threonine-, and glycine-rich protein by water stress in Lycopetsicon chilense

Genome ◽  
1996 ◽  
Vol 39 (6) ◽  
pp. 1185-1193 ◽  
Author(s):  
Long-Xi Yu ◽  
Zohreh Tabaeizadeh ◽  
Hélène Chamberland ◽  
Jean G. Lafontaine
Keyword(s):  
Cell Wall ◽  
Water Stress ◽  
Plant Cell Wall ◽  
Regulation Of Gene Expression ◽  
Down Regulation ◽  
Reading Frame ◽  
Lycopersicon Chilense ◽  
Rich Domain ◽  
Putative Signal Peptide ◽  
Glycine Rich Protein

We have isolated a full length cDNA clone (designated PTGRP) encoding a proline-rich protein from leaves of Lycopersicon chilense. Sequence analysis of the 552-bp insert revealed that the open reading frame encodes a 12.6-kDa protein. The deduced amino acid sequence of PTGRP consists of a C-terminal proline-rich domain with two identical repeat motifs Phe-Pro-Met-Pro-Thr-Thr-Pro-Ser-Thr-Gly-Gly-Gly-Phe-Pro-Ser. The N terminus lacks proline and is hydrophobic. Unlike other proline-rich proteins this protein contains five glycine-rich repeat motifs (Gly-X)n representative of glycine-rich proteins. Southern blot analysis showed that PTGRP is a member of a small gene family within the L. chilense genome. Northern blot experiments revealed that the PTGRP gene is significantly down regulated by water stress. PTGRP mRNA transcription decreased 5- to 10-fold in leaves and stems after 4–8 days of water stress. The mRNA reaccumulated when the drought-stressed plants were rewatered. The in situ hybridization experiments also revealed that PTGRP mRNAs were more abundant in leaf sections of plants watered regularly compared with those of plants submitted to water stress. Down regulation of the PTGRP gene was also observed in desiccated cell suspensions of L. chilense and in those treated with abscisic acid, mannitol, and NaCl. Based on the common features of proline-rich proteins (high proline content, repeated motifs, and a putative signal peptide) and their involvement in the cell wall, it is likely that the PTGRP protein is targeted to the cell wall. Its down regulation by drought could be correlated with the remodeling of the plant cell wall in response to water stress. Key words : proline-, threonine-, and glycine-rich protein, down regulation, drought, Lycopersicon chilense, tomato.

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