scholarly journals Use of plastic syringe as a splint for contracted fingers

2016 ◽  
Vol 9 (2) ◽  
pp. 132
Author(s):  
Amitabh Jena ◽  
GajjalaVenkata Sivanath Reddy ◽  
NaruRamana Reddy ◽  
Rashmi Patnayak ◽  
Banoth Manilal
Keyword(s):  
Plastic Syringe

Isolation and Identification of Cellular Phospholipids

1997 ◽  
Author(s):  
Donald J. Hanahan
Keyword(s):  
Venous Blood ◽  
Volume Ratio ◽  
Trisodium Citrate ◽  
Human Platelets ◽  
Convincing Evidence ◽  
Model Systems ◽  
Membrane Phospholipids ◽  
Isolation And Identification ◽  
Cellular Events ◽  
Plastic Syringe

In this chapter, some of the types of methodologies currently in use for isolation and analysis of cellular phospholipids will be outlined. Such techniques can be readily applied to experiments designed to explore the involvement of phospholipids in cellular events, such as stimulus-induced activation. Primary attention will be paid to the human platelet. If you need to justify the choice of human platelets as the cell of choice, a number of highly creditable reasons can be cited. Only three need to be considered at this point. First, the circulating platelet is of paramount importance in hemostasis, and there is convincing evidence that its membrane phospholipids are intimately involved in this process. Second, these cells can serve as excellent targets or model systems for stimulus-induced activation in which the membrane phospholipids play an important role. Third, human platelets can be isolated from whole blood by a simple, convenient centrifugal approach. Human donors are available at a very reasonable cost, and the platelets obtained from a varied spectrum of donors show remarkable consistency. Thus, one can undertake their isolation using the following method and have them available for immediate experimentation. Nonfasting venous blood is drawn (with informed consent) from male or female subjects between the ages of 20 and 40 years, who are considered to be normal and healthy and had not ingested platelet-active medication for at least 10 days prior. Blood is obtained by insertion of a butterfly infusion set (12-in. tubing from Abbott Hospitals, Inc., North Chicago, IL) with a 1-in. x 19-gauge needle into the antecubital vein. A few milliliters of blood is allowed to flow before a 60-ml plastic syringe containing 7.5 ml of an ACD [10.8% citric acid, 2.2% trisodium citrate, and 2% dextrose (w/v)] solution was attached. Four syringes were filled to the 50-ml mark and inverted gently, and the contents were transferred carefully into 50-ml plastic tubes that are then capped. The blood-to-ACD volume ratio is 6:1 (v/v). The tubes are centrifuged at 2000 rpm (830g) in a Sorvall RT 6000 centrifuge at 24°C for 15 min.

scholarly journals Minimizing Contamination from Plastic Labware in the Quantification of C16 and C18 Fatty Acids in Filter Samples of Atmospheric Particulate Matter and Their Utility in Apportioning Cooking Source Contribution to Urban PM2.5

Atmosphere ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1120
Author(s):  
Yuk Ying Cheng ◽  
Jian Zhen Yu
Keyword(s):  
Fatty Acids ◽  
Hong Kong ◽  
Particulate Matter ◽  
Fatty Acid ◽  
Stearic Acid ◽  
Palmitic Acid ◽  
New Method ◽  
Urban Site ◽  
Plastic Syringe ◽  
Syringe Filter

Palmitic acid (C16:0) and stearic acid (C18:0) are among the most abundant products in cooking emission, and thus could serve as potential molecular tracers in estimating the contributions of cooking emission to particulate matter (PM2.5) pollution in the atmosphere. Organic tracer analysis in filter-based samples generally involves extraction by organic solvents, followed by filtration. In these procedures, disposable plastic labware is commonly used due to convenience and as a precaution against sample-to-sample cross contamination. However, we observed contamination for both C16:0 and C18:0 fatty acids, their levels reaching 6–8 ppm in method blanks and leading to their detection in 9% and 42% of PM2.5 samples from Hong Kong, indistinguishable from the blank. We present in this work the identification of plastic syringe and plastic syringe filter disc as the contamination sources. We further demonstrated that a new method procedure using glass syringe and stainless-steel syringe filter holder offers a successful solution. The new method has reduced the contamination level from 6.6 ± 1.2 to 2.6 ± 0.9 ppm for C16:0 and from 8.9 ± 2.1 to 1.9 ± 0.8 ppm for C18:0 fatty acid. Consequently, the limit of detection (LOD) for C16:0 has decreased by 57% from 1.8 to 0.8 ppm and 56% for C18:0 fatty acid from 3.2 to 1.4 ppm. Reductions in both LOD and blank variability has allowed the increase in quantification rate of the two fatty acids in ambient samples and thereby retrieving more data for estimating the contribution of cooking emission to ambient PM2.5. With the assistance of three cooking related tracers, palmitic acid (C16:0), stearic acid (C18:0) and cholesterol, positive matrix factorization analysis of a dataset of PM2.5 samples collected from urban Hong Kong resolved a cooking emission source. The results indicate that cooking was a significant local PM2.5 source, contributing to an average of 2.2 µgC/m3 (19%) to organic carbon at a busy downtown roadside location and 1.8 µgC/m3 (15%) at a general urban site.

Stability of Bortezomib 1-mg/mL Solution in Plastic Syringe and Glass Vial

2005 ◽  
Vol 39 (9) ◽  
pp. 1462-1466 ◽  
Author(s):  
Pascal André ◽  
Salvatore Cisternino ◽  
Fouad Chiadmi ◽  
Audrey Toledano ◽  
Joël Schlatter ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Color Change ◽  
Hplc Method ◽  
Initial Concentration ◽  
Plastic Syringe ◽  
The Mean ◽  
The Stability ◽  
And Storage ◽  
Mean Concentration ◽  
Antineoplastic Chemotherapy

BACKGROUND: The proteasome inhibitor bortezomib (BTZ), used in antineoplastic chemotherapy, must be diluted in NaCl 0.9% for injection and stored for no more than 3 hours in a syringe or 8 hours in a vial. Better information on its stability could improve storage. OBJECTIVE: To assess the stability of BTZ solution (1 mg/mL) in syringes and vials. METHODS: BTZ 1-mg/mL solutions were prepared by adding sterile NaCl 0.9% to Velcade vials containing 3.5 mg of lyophilized BTZ. Syringes were filled with 1 mL of solution and stored in the dark at 5 °C or 60 °C; others were not protected from light and stored at 22 °C. Velcade vials containing 1 mL of solution were stored at 5 °C in the dark. Samples were taken at various times over 23 days and assayed in duplicate. An HPLC method for assaying the stability of BTZ was validated. Appearance and pH were recorded. RESULTS: There was no color change or precipitation in the samples, and the pH was stable. Oxidation, light, and storage temperature all affected the chemical stability of BTZ. The mean concentrations of BTZ in syringes stored for 2, 3, and 5 days at 60, 22, and 5 °C were >95% of the initial concentration. The mean concentration of BTZ in vials stored for 5 days at 5 °C was >95% of the initial concentration. CONCLUSIONS: BTZ stored refrigerated in vials or syringes and protected from light is chemically stable for 5 days after reconstitution.

Disposable plastic syringe

1974 ◽  
Vol 8 (4) ◽  
pp. 243-244
Author(s):  
R. I. Pertsov ◽  
V. E. Zelenetskii ◽  
G. A. Trikhanova
Keyword(s):  
Plastic Syringe ◽  
Disposable Plastic

Design, Evaluation, and Dissemination of a Plastic Syringe Clip to Improve Dosing Accuracy of Liquid Medications

2013 ◽  
Vol 41 (9) ◽  
pp. 1860-1868 ◽  
Author(s):  
Garrett J. Spiegel ◽  
Cindy Dinh ◽  
Amanda Gutierrez ◽  
Julia Lukomnik ◽  
Benjamin Lu ◽  
...  
Keyword(s):  
Design Evaluation ◽  
Plastic Syringe ◽  
Dosing Accuracy

Portable plastic syringe as a self-actuated pump for long-distance uniform delivery of liquid inside a microchannel and its application for flow-through polymerase chain reaction on chip

RSC Advances ◽  
2015 ◽  
Vol 5 (16) ◽  
pp. 12071-12077 ◽  
Author(s):  
Wenming Wu ◽  
Kieu The Loan Trinh ◽  
Yu Zhang ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Flow Rate ◽  
Chain Reaction ◽  
Long Distance ◽  
Chip Flow ◽  
Hands On ◽  
Plastic Syringe ◽  
Flow Through ◽  
Polymerase Chain ◽  
On Chip

A strategy for realizing self-actuated pumping with uniform flow rate over a long distance is introduced using hands-on operation of disposable syringe, and was applied for on-chip flow-through PCR inside a serpentine PMMA microchannel.

scholarly journals Suicide by intravenous injection of rocuronium-bromide: Case report

2005 ◽  
Vol 133 (11-12) ◽  
pp. 518-520
Author(s):  
Slobodan Nikolic ◽  
Tatjana Atanasijevic ◽  
Zorica Nesic ◽  
Andjelka Spasic
Keyword(s):  
Case Report ◽  
Intravenous Injection ◽  
The Body ◽  
Circumstantial Evidence ◽  
Forensic Investigation ◽  
Rocuronium Bromide ◽  
Left Hand ◽  
Toxicological Data ◽  
Plastic Syringe

Suicides by intravenous injection of an overdose of medicaments are uncommon. In this paper, we present the case of a suicide by rocuronium-bromide injection in combination with an oral overdose of metoprolol. Unfortunately, in Belgrade, there is no toxicological laboratory capable of detecting rocuronium. The interpretation of autopsy and toxicological data in this case was made difficult due to the extreme putrefaction of the body of the deceased. So, by forensic investigation, the case was solved indirectly, through circumstantial evidence: an empty ampoule of rocuronium found near the body, as well as a plastic syringe and cloth-bandage found in the left hand of the deceased.

The effectivity of polysaccharide extracted from marine algae, Euchema cottonii, on the immune response of tilapia, Oreochromis niloticus

2014 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Indriasari . ◽  
Remy E.P. Mangindaan ◽  
Inneke F.M. Rumengan
Keyword(s):  
Immune Response ◽  
Lateral Line ◽  
Oreochromis Niloticus ◽  
Test Material ◽  
Injection Point ◽  
Randomized Design ◽  
Test Fish ◽  
Plastic Syringe ◽  
Nonspecific Immune Response ◽  
Eucheuma Cottonii

Title (Bahasa Indonesia): Efektifitas polisakarida yang diekstrak dari alga, Euchema cottonii, terhadap respon imun ikan nila (Oreochromis niloticus)This study was aimed to test the effectiveness of polysaccharide extracts of algae, Eucheuma cottonii, in increasing the nonspecific immune response and to get an extract with best efficacy on the immune response of the tilapia, Oreochromis niloticus. The design used completely randomized design (CRD) using 4 treatments, 0 mg/kg of saline material (A), 10 mg/kg of E. cottonii extract (B), 10 mg/kg of Iota (C), and 100 cells/ml of formaline killed vaccine (FKV), Aeromonas hydrophilla, each of which were with 3 (three) replications. The fish were acclimated for 2 weeks, and then treated for 4 weeks. Test animals were vaccinated through intraperitoneal injection 2 times at one week interval as much as 0.2 ml FKV taken with disposable plastic 1 ml syringe. The vaccination was done injecting the bacteria, A. hydrophilla, previously killed in 0.4% formaldehyde FKV at the density of 109 cells / ml. Then the test fish were injected with the test material extract solution. E. cottonii as much as 0.2 ml / fish. Injection point was between the pelvic fins and the lateral line near the anus. The results showed that administration of the polysaccharide extracted from algae in tilapia gave significant effect on nonspecific immune response increment of the fish (total leukocytes and phagocytic activity). Penelitian ini bertujuan untuk menguji keefektifan ekstrak polysakarida alga laut, Eucheuma cottonii, dalam meningkatkan respons kebal non-spesifik dan untuk mendapatkan suatu ekstrak dengan kualitas terbaik terhadap respons kebal ikan nila, Oreochromis niloticus.  Penelitian ini mengguna-kan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang digunakan adalah A = 0 mg.kg-1 untuk bahan uji saline; B = 10 mg.kg-1 ekstrak alga E. cottonii; C = 10 mg.kg-1 untuk iota; D = FKV A. hydrophilla 109 sel/ml. Ikan nila pertama-tama dipelihara dalam kolam (2 x 1 m) selama 2 minggu untuk proses aklimatisasi dan kemudian diberi perlakuan selama 4 minggu. Hewan uji divaksinasi dengan injeksi secara intraperitoneal (IP) dilakukan 2 kali dengan selang waktu seminggu sebanyak 0,2 ml FKV yang diambil dengan plastic syringe disposable 1 ml. Vaksinasi dilakukan dengan menginjeksikan bakteri A. hydrophilla yang telah dimatikan dengan formalin 0,4% FKV pada kepadatan 109 sel/ml. Kemudian ikan uji disuntik dengan bahan uji larutan ekstrak E. cottonii dengan dosis penyuntikan 0,2 ml larutan/ikan. Titik suntik diantara sirip perut dan lateral line dekat anus.Hasil penelitian menunjukkan pemberian polisakarida yang diekstrak dari alga pada ikan nila memberi pengaruh yang nyata terhadap peningkatan respon imun nonspesifik ikan (total leukosit dan aktivitas fagositosis).

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