The effectivity of polysaccharide extracted from marine algae, Euchema cottonii, on the immune response of tilapia, Oreochromis niloticus

2014 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Indriasari . ◽  
Remy E.P. Mangindaan ◽  
Inneke F.M. Rumengan
Keyword(s):  
Immune Response ◽  
Lateral Line ◽  
Oreochromis Niloticus ◽  
Test Material ◽  
Injection Point ◽  
Randomized Design ◽  
Test Fish ◽  
Plastic Syringe ◽  
Nonspecific Immune Response ◽  
Eucheuma Cottonii

Title (Bahasa Indonesia): Efektifitas polisakarida yang diekstrak dari alga, Euchema cottonii, terhadap respon imun ikan nila (Oreochromis niloticus)This study was aimed to test the effectiveness of polysaccharide extracts of algae, Eucheuma cottonii, in increasing the nonspecific immune response and to get an extract with best efficacy on the immune response of the tilapia, Oreochromis niloticus. The design used completely randomized design (CRD) using 4 treatments, 0 mg/kg of saline material (A), 10 mg/kg of E. cottonii extract (B), 10 mg/kg of Iota (C), and 100 cells/ml of formaline killed vaccine (FKV), Aeromonas hydrophilla, each of which were with 3 (three) replications. The fish were acclimated for 2 weeks, and then treated for 4 weeks. Test animals were vaccinated through intraperitoneal injection 2 times at one week interval as much as 0.2 ml FKV taken with disposable plastic 1 ml syringe. The vaccination was done injecting the bacteria, A. hydrophilla, previously killed in 0.4% formaldehyde FKV at the density of 109 cells / ml. Then the test fish were injected with the test material extract solution. E. cottonii as much as 0.2 ml / fish. Injection point was between the pelvic fins and the lateral line near the anus. The results showed that administration of the polysaccharide extracted from algae in tilapia gave significant effect on nonspecific immune response increment of the fish (total leukocytes and phagocytic activity). Penelitian ini bertujuan untuk menguji keefektifan ekstrak polysakarida alga laut, Eucheuma cottonii, dalam meningkatkan respons kebal non-spesifik dan untuk mendapatkan suatu ekstrak dengan kualitas terbaik terhadap respons kebal ikan nila, Oreochromis niloticus.  Penelitian ini mengguna-kan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang digunakan adalah A = 0 mg.kg-1 untuk bahan uji saline; B = 10 mg.kg-1 ekstrak alga E. cottonii; C = 10 mg.kg-1 untuk iota; D = FKV A. hydrophilla 109 sel/ml. Ikan nila pertama-tama dipelihara dalam kolam (2 x 1 m) selama 2 minggu untuk proses aklimatisasi dan kemudian diberi perlakuan selama 4 minggu. Hewan uji divaksinasi dengan injeksi secara intraperitoneal (IP) dilakukan 2 kali dengan selang waktu seminggu sebanyak 0,2 ml FKV yang diambil dengan plastic syringe disposable 1 ml. Vaksinasi dilakukan dengan menginjeksikan bakteri A. hydrophilla yang telah dimatikan dengan formalin 0,4% FKV pada kepadatan 109 sel/ml. Kemudian ikan uji disuntik dengan bahan uji larutan ekstrak E. cottonii dengan dosis penyuntikan 0,2 ml larutan/ikan. Titik suntik diantara sirip perut dan lateral line dekat anus.Hasil penelitian menunjukkan pemberian polisakarida yang diekstrak dari alga pada ikan nila memberi pengaruh yang nyata terhadap peningkatan respon imun nonspesifik ikan (total leukosit dan aktivitas fagositosis).

scholarly journals Aplikasi Probiotik, Prebiotik dan Sinbiotik melalui Pakan untuk Meningkatkan Respon Imun dan Kelangsungan Hidup Ikan Nila Oreochromis niloticus yang Diinfeksi Streptococcus agalactiae (Application of Probiotic, Prebiotic and Synbiotic through Feed for Increasing Immune Response and Survival Rate of Nile Tilapia Oreochromis niloticus Infected by Streptococcus agalactiae)

2014 ◽  
Vol 4 (1) ◽  
pp. 15-26
Author(s):  
Widanarni Widanarni ◽  
Achmad Farouq ◽  
Munti Yuhana
Keyword(s):  
Immune Response ◽  
Survival Rate ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Positive Control ◽  
Control Fish ◽  
Fish Feed ◽  
Study Test ◽  
Test Fish

In this study, fish feed supplemented by probiotic, prebiotic and synbiotic were applied to enhance the immune response and survival rate of the fish against Streptococcus agalactiae infection. Treatments used in this study, were as follows: feed containing 1% (v/v) of probiotic (C), feed containing 2% (v/v) of prebiotic (D) and feed containing mixed of 1% (v/v) of probiotic + 2% (v/v) of prebiotic (E, defined as synbiotic). Control fish (both positive, A; and negative, B; treatments) were fed by fish feed without containing neither probiotic nor prebiotic. In this study, test fish used monosex (all male) red tilapia with the average of body weight of 13,43±2,97 gram. The fish were fed by supplemented feed within first 30 days pre injection. After that, the treated fish were challenged by 109 CFU/ml of S. agalactiae. Nile tilapia fed by synbiotic (treatment E) had higher immune response (haemoglobin, neutrofil, and phagocyte indices) than control, but were not significantly different than those of treatment C, and D. After challenged test by S. agalactiae, treatment C, D and E resulted significantly higher resistance than that of control. The survival rate of fish fed by supplemented feed containing probiotic (C), prebiotic (D) and synbiotic (E) were 76%, 76% and 80%, respectively; higher than than positive control (50%). The results showed that addition of probiotic, prebiotic and synbiotic in fish feed could increase immune response and survival rate against S. agalactiae.Keywords : tilapia, probiotic, prebiotic, synbiotic, Streptococcus agalactiae

scholarly journals POTENSI TEPUNG KULIT BUAH MANGGIS (GARCINIA MANGOSTANA L) UNTUK MENINGKATKAN PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN NILA (OREOCHROMIS NILOTICUS)

2021 ◽  
Vol 7 (1) ◽  
pp. 19-27
Author(s):  
Darna Susantie ◽  
Usy Nora Manurung
Keyword(s):  
Oreochromis Niloticus ◽  
Research Work ◽  
Fish Farming ◽  
Test Material ◽  
Fish Feed ◽  
Garcinia Mangostana ◽  
Daily Growth ◽  
Research Application ◽  
Growth And Survival ◽  
Test Fish

Ikan membutuhkan pakan dalam pertumbuhan dan kelangsungan hidupnya.  Pakan ikan adalah komponen paling penting dalam budidaya ikan.  Pakan yang berkualitas bagi ikan adalah pakan yang mudah dicerna, tidak mengandung racun, dan mengandung gizi yang tinggi.  Kulit  buah manggis dapat dijadikan pakan ikan dalam meningkatkan pertumbuhan dan kelangsungan hidup ikan yang dibudidayakan karena mengandung senyawa xantone yang cukup kuat sebagai antioksidan, antiproliferartif, dan antimicrobial.  selain itu mengandung flavonoid, saponin, alkaloid, triterpenoid, tanin, dan polifenol (Suksamrarn, 2003; Mardawati et al., 2008; Puspitasari et al., 2013).  Komposisi tepung kulit buah manggis yaitu air 9%, abu 2,58%, protein 2,69%, serat kasar 30,05%, gula total 6,92%, dan lainnya (tanin, lemak) 48,76%. Kegiatan penerapan penelitian Unggulan Perguruan Tinggi ini bertujuan untuk mengetahui pengaruh penambahan tepung kulit buah manggis terhadap pertumbuhan dan kelangsungan hidup ikan nila (Oreochromis niloticus).  Waktu pelaksanaan penelitian selama 1 bulan dari tanggal 08 Agustus sampai 10 September 2020 di Kampung Nahepese Kecamatan Manganitu.  Prosedur kerja penelitian meliputi beberapa tahap yaitu persiapan pakan uji, persiapan wadah pemeliharaan, persiapan ikan uji, dan pemeliharaan ikan uji.  Ikan uji yang digunakan dalam penelitian ini adalah ikan nila berukuran 3-5 cm sebanyak 180 ekor dimana masing-masing wadah didistribusikan  20 ekor ikan.  Sedangkan bahan uji yang dipakai adalah tepung kulit buah manggis yang ditambahkan dalam tepung pelet kemudian dicampurkan menjadi merata lalu ditambahkan air secukupnya, dicetak dan dijemur.  Laju pertumbuhan harian tertinggi ikan nila selama 30 hari pemeliharaan terdapat pada perlakuan B.  Sintasan hidup ikan nila pada kontrol dan perlakuan B yaitu 100%, yang artinya semua ikan uji yang dipelihara hidup semua.  Sedangkan pada perlakuan A yaitu 95%, dimana ada 1 ekor ikan yang meloncat keluar happa.   Fish need feed for growth and survival.  Fish feed is the most important component in fish farming.  Quality feed for fish is food that is easily digested, does not contain toxins, and contains high nutrition.  Mangosteen rind can be used as fish feed in increasing the growth and survival of cultivated fish because it contains xanthone compounds which are strong enough as antioxidants, antiproliferarts, and antimicrobials.  besides containing flavonoids, saponins, alkaloids, triterpenoids, tannins, and polyphenols (Suksamrarn, 2003; Mardawati et al., 2008; Puspitasari et al., 2013).  The composition of mangosteen rind flour is 9% water, 2.58% ash, 2.69% protein, 30.05% crude fiber, 6.92% total sugar, and 48.76% others (tannins, fat). This research application activity of Higher Education Excellence aims to determine the effect of adding mangosteen rind flour to the growth and survival of tilapia (Oreochromis niloticus).  The time for conducting the research is 1 month from August 8 to September 10 2020 in Nahepese Village, Manganitu District. The research work procedure includes several stages, namely preparation of test feed, preparation of maintenance containers, preparation of test fish, and rearing of test fish.  The test fish used in this study were 180 tilapia fish measuring 3-5 cm in which 20 fish were distributed in each container.  While the test material used is mangosteen rind flour which is added to the pellet flour then mixed evenly and then added with enough water then printed and dried. The highest daily growth rate of tilapia for 30 days of maintenance was found in treatment B. The survival rate of tilapia in control and treatment B was 100%, which means that all of the tested fish that were reared were all alive.  Whereas in treatment A, it was 95%, where 1 fish jumped out of the happa.

scholarly journals Penggunaan ragi roti (Saccharomyces cereviciae) secara in situ untuk meningkatkan respon kebal non-spesifik ikan nila (Oreochromis niloticus)

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Meilina E Rawung ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Yeast Cell ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Yeast Cells ◽  
Total Leucocyte Count ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The objetive of research was to examine the effect of yeast cell Saccharomyces cereviciae on non-specific immune response of nile tilapia, Oreochromis niloticus. Juveniles weighing 31.83 g in average were cultured in five concrete tanks with a density of 30 fish per tank. The fish were fed pellet supplemented with yeast cell at five different doses (0, 10, 20, 30, 40 g/kg pellet) for three consecutive weeks as much as 4%/bb/day, twice daily. At the end of feeding, three fish from each tank were sampled to measure the immune parameters namely total leucocyte count and phagocytosis activity of phagocyte cells. Research results showed that after three weeks of feeding, total leucocyte and phagocytosis activity of fish fed pellet supplemented with yeast cells were significantly different as compared to those of control fish.  The highest total leucocyte and phagocytosis activity were achieved in fish fed pellet supplemented with 10 g of yeast cells/kg pellet.  It was concluded that the use of yeast cells for three weeks could enhance the nonspecific immune response of nile tilapia. Keywords: Saccharomyces cerreviciae, nonspecific immune response, total leucocyte count, phagocytosis activity, nile tilapia, Oreochromis niloticus

scholarly journals Respon kebal nonspesifik ikan mas yang diberi imunostimulan ragi roti secara oral

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Aditya P. Yanuar ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Immune Parameters ◽  
North Sulawesi ◽  
Freshwater Aquaculture ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The effect of baker’s yeast as immunostimulant on innate immunity of carp (Cyprinus carpio L) had been investigated.  Fish was gathered from Freshwater Aquaculture Board (BBAT) Tatelu, Province of North Sulawesi.  The fish were acclimatized for a week in 15 aquariua with a density of 25 fish/aquarium. After acclimatization the fish were fed pellet supplemented with baker’s yeast as immunostimulant at 0, 5, 10, 15 and 20 g / kg of feed for four weeks as much as 5%/body weight of fish/day with the frequency of feeding twice a day at 08.00 am and 16.00 pm . This study used Completely Randomized Design (RAL) with five treatments and each treatment had three replications. Immune parameters consisting of total leucocytes and phagocytosis activity were measured in the last week. After  four weeks of feeding, the total leukocytes from the carp fed with the addition of baker’s yeast 10 g / kg of feed were significantly increased compared with the fish not being fed with the addition of bread yeast (p <0.01). Phagocytic activity of phagocyte cells from carp also increased significantly in fish fed pellet with the addition of 5 g of baker’s yeast / kg of feed (p = 0.01). In conclusion, the addition of baker’s yeast as immunostimulant in the diet at 5-10 g/kg of pellet can increase the nonspecific immune response of carp. Keywords:  baker’s yeast, carp, nonspesific immune response, total leucocytes, phagocytosis activity

scholarly journals PERTUMBUHAN BENIH IKAN NILA (Oreochromis niloticus) PADA BERBAGAI PADAT PENEBARAN YANG DIPELIHARA DALAM WADAH TERKONTROL

2019 ◽  
Vol 19 (2) ◽  
pp. 111
Author(s):  
Jenifer M.M. Kembuan ◽  
Deidy Y. Katili ◽  
Pience V. Maabuat
Keyword(s):  
Oreochromis Niloticus ◽  
Design Pattern ◽  
Stocking Density ◽  
Relative Growth ◽  
Randomized Design ◽  
Test Fish

Penelitian ini bertujuan untuk mengetahui padat penebaran yang cocok atau sesuai dalam mendukung pertumbuhan ikan nila. Analisis data yang digunakan yaitu ANAVA serta rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL). Meliputi benih ikan nila sebagai hewan uji yang dipelihara pada wadah plastik terdiri dari  3 perlakuan dan dan 3 kali ulangan yaitu (15 individu), (20 individu) dan  (25 individu). Hasil penelitian menunjukkan padat penebaran memberikan pengaruh terhadap pertumbuhan relatif dan biomassa benih ikan nila. Padat penebaran yang terbaik untuk mendukung pertumbuhan ikan nila adalah 15 individu perwadah sedangkan yang terendah adalah 25 individu perwadah. Mortalitas tertinggi terjadi pada padat penebaran (25 individu) sebesar 15,33 persen dan mortalitas yang paling rendah terdapat pada padat penebaran (15 individu) sebesar 0 persen.Kata Kunci : Ikan nila, padat penebaran, wadah terkontrol, pertumbuhan relatif. GROWTH OF OREOCHROMIS SEEDS (Oreochromis niloticus) IN VARIOUS STOCKING DENSITIES WHICH ARE MAINTAINED IN CONTROLLED CONTAINERS ABSTRACTThis study aimed to determine how much stocking density is suitable to support the growth of oreochromis. Analysis of the data used ANAVA and the design pattern used is a Complete Randomized Design (CRD). Test fish is oreochromis fish seeds that were kept in experimental containers consisted of 3 treatments and 3 replications, namely (15 individuals), (20 individuals) and (25 individuals). The results showed that the best stocking density that support the growth of tilapia was 15 individuals per container Plastic while the lowest was 25 individuals container Plastic, the studies results showed stocking density had an influence on the relative growth and biomass of oreochromis fish, the highest mortality occurred in treatment (density of 25 individuals) of 15,33 percent and the lowest mortality was in the (15 individuals) stocking density of 0 percent.Keywords: Oreochromis, stocking densities, controlled containers, relative growth.

scholarly journals Evaluation of The Use of Garlic (Allium sativum) in Enhancing Nonspecific Immune Response and Growth of Nile Tilapia (Oreochromis niloticus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Gercyana Marentek ◽  
Henky Manoppo ◽  
Sammy N.J Longdong
Keyword(s):  
Immune Response ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Allium Sativum ◽  
Control Fish ◽  
Total Leukocyte Count ◽  
Average Weight ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The objective of this research was to examine the effect of garlic (Allium sativum) at different doses on nonspecific immune response and growth of nile tilapia (Oreochromis niloticus). Experimental fish was nile tilapia measuring 8-15 cm with an average weight of 10.4 g per individual. Before used in the experiment, fish were reared in fiber tank for two weeks for acclimatization. After acclimatization, the fish were moved into glass aquarium (50 cm x 30 cm x 30 cm) at the density of 15 fish/aquarium. Each aquarium was equipped with aerator. Five doses of garlic as treatments used in this research consisted of A= 0 g/kg diet, B= 10 g/kg, C= 20 g/kg, D= 30 g/kg and E= 40 g/kg. Fish was fed experimental diet at a dose of 5% of body weight per day, twice a day at 08.00 and 17.00. Data collected at the end of the experiment included immune parameters (TLC and phagocytosis activity) and growth of fish. ANOVA was used to evaluate the effect of treatments on immune response and growth of fish while Duncan Test was used to evaluate the different effect between treatments. Research result showed that after four weeks of feeding, TLC of fish fed diet supplemented with garlic significantly different (p<0,01) as compared to that of control fish. The highest number of TLC was observed at treatment C (15.413x107 cell.ml-1) followed by treatment B (15.39x107 cell.ml-1), D (13,920 x 107 cell.ml-1), E (13,582 x 107 cell.ml-1) and A (12,195 x 107 cell.ml-1). TLC of fish in treatment C was different significantly compared to control as well as to other treatments. Phagocytosis activity of fish in treatment C also increased significantly (p<0,01) compared to control. The highest PA was achieved in treatment C and then treatment B, D, E and A. Growth of fish in treatment C was different significantly compared to control fish as well as other treatments. As conclusion, oral administration of garlic at 20 g/kg diet could enhance nonspecific immune response and growth of nile tilapia. Key words: Garlic, Immune response, Total leukocyte count , Phagocytosis activity, Growth

scholarly journals Toxicity of mercury (Hg) on survival and growth rate, hemato- and histopathological parameters ofOreochromis niloticus

2013 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Kukuh Nirmala ◽  
Yuni Puji Hastuti ◽  
Vika Yuniar
Keyword(s):  
Growth Rate ◽  
Oreochromis Niloticus ◽  
White Blood Cells ◽  
Survival Rates ◽  
Aquatic Organisms ◽  
Growth And Survival ◽  
Randomized Design ◽  
Test Fish ◽  
Survival And Growth ◽  
Haemoglobin Content

<p>Heavy metals are serious pollutants of the aquatic environment because of their environmental persistence and ability to be accumulated by aquatic organisms. <em>Oreochromis niloticus </em>exposed to 0, 0.16, 0.5, and 1.0 ppm Hg for 30 days. The aim of this study was to determine the influence of mercury in water on survival rate, growth rates, hematological, and histological parameters of <em>Oreochromis niloticus</em>. This study was conducted from Mei to June 2009. The experimental design was arranged in completely randomized design with four treatments and three replications. Stock density was 8 fish/aquarium with mean initial body weight was 15.70±1.13 g. Growth and survival rates of test fish were decreased with increasing the Hg concentration. Red blood cell (RBC) count, haematocrit content, and haemoglobin content decreased when compared to the control. The number of white blood cells (WBC) increased in mercuric treated fish. The results are statistically significant at p&lt;0.05 level.</p> <p>Keywords:mercury, survival and growth rate, hematology, histopathology, <em>Oreochromis niloticus</em></p>

NATURAL FEEDING OF SLUDGE WORMS (Tubifex sp.) WITH DIFFERENT FEEDING RATE ON GROWTH AND SURVIVAL RATE OF CTENOPOMA (Ctenopoma acutirostre) FISH SEEDS

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Muhammad Atilah Setiawan ◽  
Muarif Muarif ◽  
Fia Sri Mumpuni
Keyword(s):  
Survival Rate ◽  
Feeding Rate ◽  
Specific Growth ◽  
Feeding Frequency ◽  
Initial Weight ◽  
Growth And Survival ◽  
Randomized Design ◽  
Absolute Length ◽  
Test Fish ◽  
Completely Randomized Design

The aim of this study was to determine the growth and survival rate of Ctenopoma fish seeds. This study used a completely randomized design with 3 treatments and 3 replications. The treatment in this study was a different feeding rate using natural feed of Tubifex sp  namely A (8%), B (10%), and C (12%) ). The test fish used was Ctenopoma fish seeds with an initial length of 2.40 ± 0.10 cm and an initial weight of 0.32 ± 0.05 g. Fish seeds are cultivated  for 28 days with feeding frequency twice a day. The results of research showed that the use of different feeding rate had an significantly different (P<0.05) on the specific growth  rate and absolute length growth with the best results in the treatment C. The survival rate was not significantly different between treatments (P>0.05). Key Words: Ctenopoma, feeding rate, growth, survival rate 

Sign in / Sign up

Export Citation Format

Share Document