Antidiabetic activity and phytochemical screening of crude extract of Stevia rebaudiana in alloxan-induced diabetic rats

Abutilon indicum(L.) Sweet is an Asian phytomedicine traditionally used to treat several disorders, including diabetes mellitus. However, molecular mechanisms supporting the antidiabetic effect ofA. indicumL. remain unknown. The aim of this study was to evaluate whether extract ofA. indicumL. improves insulin sensitivity. First, we observed the antidiabetic activity of aqueous extract of the entire plant (leaves, twigs and roots) ofA. indicumL. on postprandial plasma glucose in diabetic rats. The subsequent experiments revealed that butanol fractions of the extract bind to PPARγand activate 3T3-L1 differentiation. To measure glucose uptake enhanced by insulin-like activity, we used rat diaphragm incubated with various concentrations of the crude extract and found that the extract enhances glucose consumption in the incubated solution. Our data also indicate that the crude extract and the fractions (water and butanol) did not affect the activity of kinases involved in Akt and GSK-3βpathways; however, the reporter assay showed that the crude extract could activate glucose transporter 1 (GLUT1) promoter activity. These results suggest that the extract fromA. indicumL. may be beneficial for reducing insulin resistance through its potency in regulating adipocyte differentiation through PPARγagonist activity, and increasing glucose utilization via GLUT1.

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Evaluation of Antidiabetic Property of Sansevieria liberica Gerald and Labroy (Dracaenaceae) Leaf Using Alloxan Induced Diabetes Model

Nigerian Journal of Pharmaceutical Research ◽

10.4314/njpr.v16i2.9s ◽

2021 ◽

Vol 16 (2) ◽

pp. 73-84

Author(s):

H.M.N. Ifebi ◽

F.A. Onyegbule ◽

C.C. Ezea ◽

S.O. Ifenatuora ◽

F.C. Anowi

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Crude Extract ◽

Antidiabetic Activity ◽

Phytochemical Screening ◽

Glucose Levels ◽

Standard Procedures ◽

Traditional Remedy ◽

Diabetes Model

Background: The phytochemical screening and antidiabetic activities of the methanol leaf extract and fractions of Sansevieria liberica Ger. and Labr. were investigated.Objectives: To identify the phytoconstituents present and evaluate the anti-diabetic activity of the leaf of S. liberica Ger. and Labr.Material and Methods: A 1.2 g of the pulverized leaf was cold macerated in methanol to obtain 240 g of the crude extract (CE), fractionation was done using n-hexane, ethylacetate and butanol. The CE and the fractions were screened for phytochemical and antidiabetic analysis using standard procedures. Diabetes was induced by intraperitonial injection of alloxan. The blood glucose levels were analyzed as indices of diabetes.Results: After 24 hours, alloxan increased the blood glucose level of rats indicating hyperglycaemia. Treatment of the rats with the extract and fractions reduced the blood glucose level within 3 to 7 days of treatment. The standard antidiabetic, metformin (500 mg/kg) also showed similar effect. Extracts (250 and 500 mg/kg) of the crude extract decreased significantly (p<0.05) the blood glucose level. The antidiabetic potency of the extract and fractions were in the order of CE ˃ ethylacetate fraction > n-Hexane fraction > butanol fraction. The phytochemical screening of the extract and fractions revealed the presence of flavonoids, steroids, alkaloids, glycosides etc.Conclusions: From the findings, S. liberica leaf posses antidiabetic activity which may be due to the phytochemical constituents present, and this justifies its use by local users as traditional remedy for diabetes mellitus.

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Antidiabetic activity and phytochemical screening of Acalypha wilkesiana (Euphorbiaceae) Mull Arg. roots in alloxan-induced diabetic rats

Scientific Research and Essays ◽

10.5897/sre2014.5824 ◽

2014 ◽

Vol 9 (7) ◽

pp. 204-212 ◽

Cited By ~ 1

Author(s):

U. E. Odoh ◽

R. I. Ndubuokwu ◽

S. I. Inya-Agha ◽

P. O. Osadebe ◽

P. F. Uzor ◽

...

Keyword(s):

Diabetic Rats ◽

Antidiabetic Activity ◽

Phytochemical Screening ◽

Acalypha Wilkesiana

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Evaluation of antidiabetic activity of aqueous extract of Origanum floribundum Munby leaves in alloxan induced diabetic rats wistar

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale ◽

10.4081/jbr.2020.9141 ◽

2021 ◽

Vol 93 (2) ◽

Author(s):

M'hamed Nasri ◽

Mohamed Zaouani ◽

Noura Mimoune ◽

Fatma Amira Hani ◽

Mohamed Mahdid ◽

...

Keyword(s):

Acute Toxicity ◽

Aqueous Extract ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Albino Rats ◽

Phytochemical Screening ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Standard Procedures ◽

Wistar Albino Rats

The present study aimed to evaluate the phytochemicals and the antidiabetic properties of Origanum floribundum aqueous extract. Phytochemical screening was conducted by using various standard procedures. Acute toxicity of the extract was determined by OECD guidelines 423. The antidiabetic activity of the plant was determined by alloxan-induced diabetes in Wistar albino rats. Data obtained showed the detection of various secondary metabolites such as alkaloids, flavonoids, saponins, terpenoids in the extract. No mortality was observed during acute toxicity studies up to the dose of 2000 mg/kg. The aqueous extract presented the ability of reducing blood glucose levels. These findings indicated that Origanum floribundum aqueous extract had various phytopharmacological activities and thus it would be useful to isolate and characterize the compounds responsible for these bioactivities in the future.

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Hypoglycemic and antidiabetic activity of leaves of Aegle marmelose in normoglycemic and streptozotocin induced diabetic rats

Planta Medica ◽

10.1055/s-0028-1084306 ◽

2008 ◽

Vol 74 (09) ◽

Author(s):

AR Juvekar ◽

DD Bandawane ◽

MP Kulkarni ◽

MR Juvekar

Keyword(s):

Diabetic Rats ◽

Antidiabetic Activity

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Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽

10.33307/entomon.v44i2.438 ◽

2019 ◽

Vol 44 (2) ◽

pp. 117-126

Author(s):

Rhitayu Chakraborti ◽

Probir Kumar Bandyopadhyay

Keyword(s):

Ethyl Acetate ◽

Bioactive Compounds ◽

Active Ingredient ◽

Crude Extract ◽

Ethyl Acetate Extract ◽

Lantana Camara ◽

Mosquito Larvae ◽

Aedes Triseriatus ◽

Phytochemical Screening ◽

Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

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Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

International Journal of Pharmaceutical and Phytopharmacological Research ◽

10.24896/eijppr.2016616 ◽

2017 ◽

Vol 6 (1) ◽

pp. 34

Author(s):

Michael Russelle Alvarez ◽

Paolo Robert Bueno ◽

Raymond Oliver Cruz ◽

Richard Macapulay ◽

Francis Jayson Vallesfin ◽

...

Keyword(s):

Crude Extract ◽

Carica Papaya ◽

Uv Light ◽

Digestive Enzyme ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Salivary Amylase ◽

Garcinia Mangostana ◽

Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

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Antidiabetic Activity of Pistia strateotes L.Aqueous Extract in Alloxan-induced Diabetic Rats

10.26538/tjnpr/v3i3.5 ◽

2019 ◽

Vol 3 (3) ◽

pp. 91-94

Author(s):

Mudassir Lawal ◽

◽

Abdulaziz Suleiman ◽

Nasiruddin Matazu ◽

Fatima Dawud ◽

...

Keyword(s):

Diabetic Rats ◽

Antidiabetic Activity

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