Dental pulp stem cells-derived schwann cells for peripheral nerve injury regeneration

This review summarizes results from in vitro and in vivo studies which provide evidence that human dental pulp stem cells (hDPSCs) might be a novel treatment strategy for nervous system injuries and neurodegenerative diseases because of their high potential for neurogenic differentiation and secretion of neuron-related trophic factors. It is also worth underlining that hDPSCs are neural crest-derived cells that possess biological properties of mesenchymal stem cells (MSCs). Induced hDPSCs have a high ability to differentiate into neuron-like cells, which show functional activity. hDPSCs express immunomodulatory factors that enhance regeneration and repair of nerve injury. These specific features of undifferentiated and differentiated hDPSCs make these cells promising for the therapy of neurodegenerative diseases, such as Alzheimer’s, Parkinson’s diseases, stroke, spinal cord injury as well as peripheral nerve injury. Recently, investigators propose that the tissue engineering technology, including scaffold, stem cells and growth factor, should provide a new strategy for spinal cord and peripheral nerve injury treatment. hDPSCs should be considered as a good choice for peripheral nerve injury therapy, because they have better potential to differentiate into neural and glial cells than stem cells coming from other sources through the expression of neuronal makers and wide range of neurotropic factors secretion. Unique properties of hDPSCs, such as high proliferation rate, trophic factors expression and stronger neuroprotective effects, indicate that these stem cells may be beneficial in neural disease therapy.

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Past, Present, and Future of Nerve Conduits in the Treatment of Peripheral Nerve Injury

BioMed Research International ◽

10.1155/2015/237507 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 70

Author(s):

Aikeremujiang Muheremu ◽

Qiang Ao

Keyword(s):

Stem Cells ◽

Clinical Practice ◽

Peripheral Nerve ◽

Schwann Cells ◽

Nerve Injury ◽

Neurotrophic Factors ◽

Peripheral Nerve Injury ◽

Functional Outcomes ◽

Nerve Conduits ◽

New Construction

With significant advances in the research and application of nerve conduits, they have been used to repair peripheral nerve injury for several decades. Nerve conduits range from biological tubes to synthetic tubes, and from nondegradable tubes to biodegradable tubes. Researchers have explored hollow tubes, tubes filled with scaffolds containing neurotrophic factors, and those seeded with Schwann cells or stem cells. The therapeutic effect of nerve conduits is improving with increasing choice of conduit material, new construction of conduits, and the inclusion of neurotrophic factors and support cells in the conduits. Improvements in functional outcomes are expected when these are optimized for use in clinical practice.

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Perspective on Schwann Cells Derived from Induced Pluripotent Stem Cells in Peripheral Nerve Tissue Engineering

Cells ◽

10.3390/cells9112497 ◽

2020 ◽

Vol 9 (11) ◽

pp. 2497

Author(s):

Zhong Huang ◽

Rebecca Powell ◽

James B. Phillips ◽

Kirsten Haastert-Talini

Keyword(s):

Stem Cells ◽

Schwann Cell ◽

Peripheral Nerve ◽

Induced Pluripotent Stem Cells ◽

Schwann Cells ◽

Nerve Injury ◽

Pluripotent Stem Cells ◽

Peripheral Nerve Injury ◽

Induced Pluripotent

Schwann cells play a crucial role in successful peripheral nerve repair and regeneration by supporting both axonal growth and myelination. Schwann cells are therefore a feasible option for cell therapy treatment of peripheral nerve injury. However, sourcing human Schwann cells at quantities required for development beyond research is challenging. Due to their availability, rapid in vitro expansion, survival, and integration within the host tissue, stem cells have attracted considerable attention as candidate cell therapies. Among them, induced pluripotent stem cells (iPSCs) with the associated prospects for personalized treatment are a promising therapy to take the leap from bench to bedside. In this critical review, we firstly focus on the current knowledge of the Schwann cell phenotype in regard to peripheral nerve injury, including crosstalk with the immune system during peripheral nerve regeneration. Then, we review iPSC to Schwann cell derivation protocols and the results from recent in vitro and in vivo studies. We finally conclude with some prospects for the use of iPSCs in clinical settings.

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Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury

CNS Neuroscience & Therapeutics ◽

10.1111/cns.13187 ◽

2019 ◽

Vol 26 (2) ◽

pp. 189-196 ◽

Cited By ~ 12

Author(s):

Cai‐Yue Liu ◽

Gang Yin ◽

Yi‐Dan Sun ◽

Yao‐Fa Lin ◽

Zheng Xie ◽

...

Keyword(s):

Stem Cells ◽

Peripheral Nerve ◽

Schwann Cells ◽

Nerve Injury ◽

Peripheral Nerve Injury ◽

Adipose Derived Stem Cells

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Dental pulp-derived stem cells can counterbalance peripheral nerve injury-induced oxidative stress and supraspinal neuro-inflammation in rat brain

Scientific Reports ◽

10.1038/s41598-018-34151-x ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 8

Author(s):

Imran Ullah ◽

Yong-ho Choe ◽

Mehtab Khan ◽

Dinesh Bharti ◽

Sharath Belame Shivakumar ◽

...

Keyword(s):

Oxidative Stress ◽

Stem Cells ◽

Peripheral Nerve ◽

Rat Brain ◽

Nerve Injury ◽

Dental Pulp ◽

Peripheral Nerve Injury ◽

Neuro Inflammation

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Trophic Effects of Dental Pulp Stem Cells on Schwann Cells in Peripheral Nerve Regeneration

Cell Transplantation ◽

10.3727/096368915x688074 ◽

2016 ◽

Vol 25 (1) ◽

pp. 183-193 ◽

Cited By ~ 30

Author(s):

Tsubasa Yamamoto ◽

Yohei Osako ◽

Masataka Ito ◽

Masashi Murakami ◽

Yuki Hayashi ◽

...

Keyword(s):

Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Schwann Cells ◽

Dental Pulp ◽

Peripheral Nerve Regeneration ◽

Dental Pulp Stem Cells ◽

Trophic Effects

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Biological Function and Mechanism of Bone Marrow Mesenchymal Stem Cells-packed Poly (3,4-ethylenedioxythiophene) (PEDOT) Scaffolds for Peripheral Nerve Injury: The Involvement of miR-21-Notch Signaling Pathway

Current Neurovascular Research ◽

10.2174/1567202614666161123112832 ◽

2017 ◽

Vol 14 (1) ◽

pp. 19-25 ◽

Cited By ~ 4

Author(s):

Wenliang Wu ◽

Shijun Zhang ◽

Yunzhen Chen ◽

Haichun Liu

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Peripheral Nerve ◽

Notch Signaling ◽

Nerve Injury ◽

Peripheral Nerve Injury ◽

Biological Function ◽

Notch Signaling Pathway ◽

Marrow Mesenchymal Stem Cells

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Transplantation of Schwann cells in a collagen tube for the repair of large, segmental peripheral nerve defects in rats

Journal of Neurosurgery ◽

10.3171/2013.4.jns121189 ◽

2013 ◽

Vol 119 (3) ◽

pp. 720-732 ◽

Cited By ~ 30

Author(s):

Yerko A. Berrocal ◽

Vania W. Almeida ◽

Ranjan Gupta ◽

Allan D. Levi

Keyword(s):

Sciatic Nerve ◽

Peripheral Nerve ◽

Schwann Cells ◽

Nerve Injury ◽

Peripheral Nerve Injury ◽

Fluorescent Protein ◽

Control Groups ◽

Myelinated Axons ◽

Segmental Nerve ◽

Green Fluorescent

Object Segmental nerve defects pose a daunting clinical challenge, as peripheral nerve injury studies have established that there is a critical nerve gap length for which the distance cannot be successfully bridged with current techniques. Construction of a neural prosthesis filled with Schwann cells (SCs) could provide an alternative treatment to successfully repair these long segmental gaps in the peripheral nervous system. The object of this study was to evaluate the ability of autologous SCs to increase the length at which segmental nerve defects can be bridged using a collagen tube. Methods The authors studied the use of absorbable collagen conduits in combination with autologous SCs (200,000 cells/μl) to promote axonal growth across a critical size defect (13 mm) in the sciatic nerve of male Fischer rats. Control groups were treated with serum only–filled conduits of reversed sciatic nerve autografts. Animals were assessed for survival of the transplanted SCs as well as the quantity of myelinated axons in the proximal, middle, and distal portions of the channel. Results Schwann cell survival was confirmed at 4 and 16 weeks postsurgery by the presence of prelabeled green fluorescent protein–positive SCs within the regenerated cable. The addition of SCs to the nerve guide significantly enhanced the regeneration of myelinated axons from the nerve stump into the proximal (p < 0.001) and middle points (p < 0.01) of the tube at 4 weeks. The regeneration of myelinated axons at 16 weeks was significantly enhanced throughout the entire length of the nerve guide (p < 0.001) as compared with their number in a serum–only filled tube and was similar in number compared with the reversed autograft. Autotomy scores were significantly lower in the animals whose sciatic nerve was repaired with a collagen conduit either without (p < 0.01) or with SCs (p < 0.001) when compared with a reversed autograft. Conclusions The technique of adding SCs to a guidance channel significantly enhanced the gap distance that can be repaired after peripheral nerve injury with long segmental defects and holds promise in humans. Most importantly, this study represents some of the first essential steps in bringing autologous SC-based therapies to the domain of peripheral nerve injuries with long segmental defects.

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