Antimicrobial resistance pattern of Pseudomonas aeruginosa isolated from various clinical samples in a tertiary care hospital, South Odisha, India

Pseudomonas aeruginosa is one of the most widespread gram-negative microorganisms identified in the clinical samples and most common causes of hospital acquired infection. P. aeruginosa is affecting both indoor and outdoor patients throughout the world. Due to frequent mutation in P. aeruginosa highly resistant strain developed rapidly. The aim of the study to determine the prevalence of P. aeruginosa species in different samples isolated from a Tertiary care Hospital as well as determination their diverse antibiotic resistance pattern. This cross-sectional study was carried out to determine in-vitro resistance pattern of P. aeruginosa isolates to common antimicrobial agents by disc diffusion method. Various clinical samples were collected from Bangladesh Health Sciences Hospital (BIHS) General Hospital, Dhaka. This research was carried out in the Department of Microbiology of Bangladesh University of Health Sciences (BUHS). Isolation, identification and antibiogram were performed for P. aeruginosa following standard microbiological laboratory procedure. A total of 218 P. aeruginosa were isolated from 3062 different clinical specimens which are statistically significant (p<0.0001). Among the highest number of P. aeruginosa were isolated from outdoor patients 140 compare to Indoor patients which are significantly higher (p <0.013). In this study Male (68.3%) are more vulnerable to P. aeruginosa infection compare to females (31.7%) which is also statistically significant. Young people (less than 35 years) were more susceptible to P. aeruginosa infection which is also statistically significant (p< 0.01). The highest number of P. aeruginosa was isolated from wound (43.12%), followed by pus (40.33%), sputum (8.71%) urine (7.80%). The maximum number of P. aeruginosa in various samples was resistant to aztreonam and co-tromoxazole followed by cephalosporins, aminoglycosides, carbapenems. The most sensitive antibiotic was colistin of followed by gentamycin and tetracycline. To control the spread of resistant bacteria, it is disparagingly vital to have stringent antibiotic guidelines. The antibiotic susceptibility pattern of P. aeruginosa requires to be continuously monitored in specialized clinical units and the results readily made available to the clinicians to minimize the resistance.

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Isolation of Pseudomonas aeruginosa from various Clinical Isolates and it Antimicrobial Resistance Pattern in a Tertiary Care Hospital

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2016.503.030 ◽

2016 ◽

Vol 5 (3) ◽

pp. 247-253

Author(s):

Shipra Saroj Golia ◽

Suhani, S. Manasa Jyoti

Keyword(s):

Pseudomonas Aeruginosa ◽

Antimicrobial Resistance ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Clinical Isolates ◽

Resistance Pattern ◽

Care Hospital

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Path-Organism Burden and Antibiogram Outline of Pseudomonas aeruginosa Isolates in a Tertiary Care Hospital of Jamshedpur, Jharkhand, India

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2021/125 ◽

2021 ◽

Vol 8 (11) ◽

pp. 638-642

Author(s):

Mohammad Mustaqueem ◽

Vindeshwar Mahto ◽

Chandan Kumar Poddar ◽

Rita Chouhan ◽

Prity Mohan Kumar ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Antimicrobial Resistance ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Control Measures ◽

Clinical Samples ◽

Care Hospital ◽

Cross Sectional ◽

Significant Growth ◽

Medical College

BACKGROUND Pseudomonas aeruginosa is an opportunistic pathogen involved in a variety of nosocomial infections like pneumonia, bacteraemia, wound infection and urinary tract infection. It is also involved in infections of rigorous burns and infections in immunocompromised persons. This study was undertaken to determine the prevalence and antibiotic susceptibility patterns of pathogenic P. aeruginosa isolated from a variety of clinical specimens in a tertiary care hospital of Jamshedpur, Jharkhand, India. METHODS Pseudomonas aeruginosa was identified using standard methods from various clinical samples collected over a period of seven months. This was a descriptive cross-sectional study which was approved by the ethical committee. The study was conducted from January 2019 to January 2020 in the Department of Microbiology at MGM Medical College, Jamshedpur, Jharkhand, India. This hospital has ICUs, one emergency ward, surgical & medical wards and Out-Patient Departments. RESULTS Our study showed the prevalence of P. aeruginosa during the study period from January 2019 to January 2020 in the Department of Microbiology at MGM Medical College, Jamshedpur, Jharkhand, India. A total of 1389 clinical samples were aerobically cultured, out of which 758 (54.6 %) yielded significant growth and the rest 630 (45.4 %) samples were either sterile or showed non-significant growth. From 758 positive growth samples, 161 (21.20 %) P. aeruginosa were isolated CONCLUSIONS The high prevalence of P. aeruginosa as an opportunistic nosocomial pathogen and high frequency of antimicrobial resistance among the clinical isolates demand regular monitoring of antibiogram of P. aeruginosa isolates with proper implementation of antimicrobial policy. Antibiotics should be used appropriately with care. Antimicrobial therapy should not be started unless there is clear evidence of infection and infection to be handled with proper infection control measures. KEYWORDS Pseudomonas aeruginosa, Pus Samples, Antimicrobial Resistance

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