Genetic differentiation of predominant mosquito species in Hainan province and characterization of mosquito midgut microbiota

2021 ◽  
Vol 1 (1) ◽  
pp. 37
Author(s):  
Qianfeng Xia ◽  
Xun Kang ◽  
Biao Liu ◽  
Siping Li
Keyword(s):  
Genetic Differentiation ◽  
Mosquito Species ◽  
Hainan Province ◽  
Mosquito Midgut

scholarly journals Seasonal abundance and larval habitats characterization of mosquito species in Lagos State, Nigeria

2020 ◽  
Vol 10 ◽  
pp. e00656
Author(s):  
Ifeoluwa Kayode Fagbohun ◽  
Emmanuel Taiwo Idowu ◽  
Taiwo Samson Awolola ◽  
Olubunmi Adetoro Otubanjo
Keyword(s):  
Mosquito Species ◽  
Seasonal Abundance ◽  
Larval Habitats ◽  
Lagos State

scholarly journals The environment and species affect gut bacteria composition in laboratory co-cultured Anopheles gambiae and Aedes albopictus mosquitoes

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sally A. Saab ◽  
Heinrich zu Dohna ◽  
Louise K. J. Nilsson ◽  
Piero Onorati ◽  
Johnny Nakhleh ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Aedes Albopictus ◽  
Food Source ◽  
Mosquito Species ◽  
Critical Role ◽  
Microbiota Composition ◽  
Human Pathogens ◽  
Multiple Factors ◽  
Mosquito Midgut ◽  
Environmental Contribution

AbstractThe midgut microbiota of disease vectors plays a critical role in the successful transmission of human pathogens. The environment influences the microbiota composition; however, the relative mosquito-species contribution has not been rigorously disentangled from the environmental contribution to the microbiota structure. Also, the extent to which the microbiota of the adult sugar food source and larval water can predict that of the adult midgut and vice versa is not fully understood. To address these relationships, larvae and adults of Anopheles gambiae and Aedes albopictus were either reared separately or in a co-rearing system, whereby aquatic and adult stages of both species shared the larval water and sugar food source, respectively. Despite being reared under identical conditions, clear intra- and interspecies differences in midgut microbiota-composition were observed across seven cohorts, collected at different time points over a period of eight months. Fitting a linear model separately for each OTU in the mosquito midgut showed that two OTUs significantly differed between the midguts of the two mosquito species. We also show an effect for the sugar food source and larval water on the adult midgut microbiota. Our findings suggest that the mosquito midgut microbiota is highly dynamic and controlled by multiple factors.

Characterization of a unique human single-chain antibody isolated by phage-display selection on membrane-bound mosquito midgut antigens

2002 ◽  
Vol 261 (1-2) ◽  
pp. 73-83 ◽  
Author(s):  
Brian D Foy ◽  
Gerry F Killeen ◽  
Ross H Frohn ◽  
Daniel Impoinvil ◽  
Andrew Williams ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Mosquito Midgut ◽  
Midgut Antigens ◽  
Membrane Bound

Genotypic and phenotypic characterization of genetic differentiation and diversity in the USDA rice mini-core collection

Genetica ◽  
2010 ◽  
Vol 138 (11-12) ◽  
pp. 1221-1230 ◽  
Author(s):  
Xiaobai Li ◽  
Wengui Yan ◽  
Hesham Agrama ◽  
Biaolin Hu ◽  
Limeng Jia ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Core Collection ◽  
Phenotypic Characterization ◽  
Mini Core Collection

scholarly journals The P25 Ookinete Surface Proteins: Homology Modeling and Phylogenetic Relationships

2013 ◽  
Vol 2013 ◽  
pp. 1-17
Author(s):  
Babita Sharma ◽  
Manoj Kumar Jaiswal
Keyword(s):  
Phylogenetic Relationships ◽  
Surface Protein ◽  
Surface Proteins ◽  
Parasite Development ◽  
Transmission Blocking ◽  
Malaria Burden ◽  
Mosquito Midgut ◽  
Egf Domain ◽  
Domain Iii

Sexual stages of Plasmodium such as zygote, ookinete, and young oocysts express 25 kDa surface protein P25, which along with P28 proteins protect the parasite from harmful environment inside mosquito midgut. Vaccines against these proteins induce antibodies in vertebrate host capable to inhibit parasite development in mosquito midgut and thus preventing the transmission of parasite from mosquito to other human host. Transmission-blocking vaccines help reduce malaria burden. The purpose of this study was in silico structural characterization of P25 family proteins and to predict their phylogenetic relationships with other proteins. Results indicate that members of P25 family have four EGF domains arranged in triangular fashion with major variations lying in the loop regions. All 22 cysteines are conserved forming 11 disulphide bonds. The C-loop of EGF domain IV in P25 proteins is smaller in comparison to P28 proteins. B loop of EGF domain II showed maximum RMSD variations followed by loops of EGF domain III. P25 proteins are tile-like triangular flat proteins that protect the parasite inside mosquito midgut. Obtained structures will help in understanding the biology of the parasite inside the mosquito midgut. These structures may also help in designing transmission-blocking vaccine against malaria in absence of experimentally determined structures.

scholarly journals Genetic Differentiation of Clariid Populations using Microsatellite Markers in Kano State Rivers

2021 ◽  
pp. 35-45
Author(s):  
I. O. Suleiman ◽  
R.O. Okeke ◽  
J. M. Madu ◽  
A. U. Umar ◽  
O.M Akinsola ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Differentiation ◽  
Dna Extraction ◽  
Genetic Characterization ◽  
Caudal Peduncle ◽  
Fta Cards ◽  
Fish Samples ◽  
Polymerase Chain ◽  
Outbred Populations

This study aimed to investigate the genetic characterization of strains of Clariid fish species in some river bodies in Kano State using microsatellite markers.One hundred and seventy seven Clariid fish samples (Clariasgariepinus and Heterobranchuslongifilis) were collected from six rivers (Thomas, Ghari, Tiga dam, Duddurun Gaya, Karaye and Bagwai) in Kano state. Blood sample was taken from each fish sample by severing the caudal peduncle and drained into FTA cards for DNA extraction, Polymerase Chain Reaction and electrophoresis to determine genetic variation between the Clariid fish populations.Genealex 6.4 software package was used to analyse the resolve bands from DNA extraction to determine their base pair and genetic variation. Results showed that the Fst values ranged from 0.00 to 0.66, Fit ranged from -0.04 to 0.12, Fis ranged from -0.35 to -0.26. It indicated a large number of gene flow (exchange) among the populations with a range of 0.46 to 0.87. There was an established magnitude of genetic divergence (91.86%) among the populations as shown by the result of the percentage polymorphism which depends on the number of alleles detected per locus and their frequencies. It can be concluded that since there was no inbreeding as shown in the study, none of the population exhibited genetic uniqueness. The populations had a high genetic differentiation between populations but moderate differentiation within populations. The populations were outbred populations; an indication that relatives avoided mating in the population.

scholarly journals Genetic differentiation of Norway spruce (Picea abies (L.) Karst.) trees with diferent crown types from the mountain Golija

Genetika ◽  
2015 ◽  
Vol 47 (3) ◽  
pp. 849-861 ◽  
Author(s):  
Vladislava Galovic ◽  
Mirjana Sijacic-Nikolic ◽  
Robert Safhauzer ◽  
Dijana Cortan ◽  
Sasa Orlovic
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Picea Abies ◽  
Genetic Differentiation ◽  
Norway Spruce ◽  
Plant Species ◽  
Genetic Characterization ◽  
Similarity Matrix ◽  
Primer Sets

The knowledge of genetic diversity degree of given species is of great importance for the successful process of breeding and genetic conservation. The aim of conducted research was to determine the genetic differentiation of Norway spruce (Picea abies (L.) Karst) genotypes with very specific narrow pyramidal and normal crown type, which grows at different altitude of the mountain Golija. For assessment of genetic similarities or differences between studied genotypes co-dominant microsatellite system had been used. This system has proven to be reliable and efficient in the genetic characterization of plant species. In total 22 primer sets have been tested, while 16 (73%) of them resulted in the successful yield of the amplified product. The analysis show that studied individuals had in total 130 alleles, in average 8.125 polymorphic alleles per each locus. The lowest polymorphism was detected in the locus EATC1D10, EATC1F03B and EATC2G09, while the highest level of polymorphism was detected in EATC2G08. Based on microsatellite date and similarity matrix, cluster analysis dendrogram indicates existence of the vertical differentiation of studied genotypes, which is consistent with results of previous Norway spruce studies.

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