scholarly journals Spectrum of clinicohematological profile and its correlation with average parasite density in visceral leishmaniasis

CytoJournal ◽  
2018 ◽  
Vol 15 ◽  
pp. 19
Author(s):  
Vijay Kumar ◽  
Poojan Agarwal ◽  
Sadhna Marwah ◽  
A. S. Nigam ◽  
Awantika Tiwari
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Parasite Density ◽  
Plasma Cells ◽  
Parasite Load ◽  
Statistical Correlation ◽  
Positive Trend ◽  
Strong Negative Correlation ◽  
Cell Percentage ◽  
Bone Marrow Plasma

Background: Leishmaniasis is the prevalent in tropical and subtropical regions of the world. Demonstration of Leishman-Donovan (LD) bodies in the bone marrow aspirates (BMA) is vital to diagnosis of visceral leishmaniasis (VL). In the present study, we studied the clinicohematological parameters encountered in VL and correlated them with parasite load on BMA. Methods: Retrospective analysis over 3 years was done; clinical details, biochemical profile, complete hemogram with peripheral smear findings, and BMA smears were reviewed and average parasite density (APD) calculated in each case. Multivariate analysis and tests of significance were applied. Results: The study included 28 patients. Splenomegaly showed a positive trend with APD. rK39 antigen detection test was 100% positive in select cases. A strong negative correlation was observed between albumin to globulin ratio and grade of APD. BMA revealed hemophagocytosis (HPS) in 78.57% cases and it had a significant strong correlation with APD (P = 0.014). A significant correlation was also observed between APD and bone marrow plasma cell percentage (P = 0.01). LD bodies were noted in unusual locations such as within myelocytes (14.2%), plasma cells (7.1%), and megakaryocytes (10.7%). Conclusion: HPS and bone marrow plasmacytosis were two statistically significant findings, which showed positive correlation with parasite load. The presence of these two findings should prompt hematopathologists for more focused search of hemoparasites in BMA to arrive at a definitive diagnosis. This will avoid unnecessary workups and improve the prognosis. To the best of our knowledge, a statistical correlation between APD and clinicohematological parameters has never been previously studied.

scholarly journals Hematological profile in visceral leishmaniasis

2013 ◽  
Vol 2 (2) ◽  
pp. 39-44 ◽  
Author(s):  
Y Agrawal ◽  
AK Sinha ◽  
P Upadhyaya ◽  
SU Kafle ◽  
S Rijal ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Plasma Cells ◽  
Parasite Load ◽  
Clinical History ◽  
Bone Marrow Examination ◽  
Severe Form ◽  
P Value ◽  
Cross Sectional ◽  
Haematological Profile

INTRODUCTION: Visceral Leishmaniasis is the most severe form of leishmaniasis and can be fatal in the absence of treatment. Nepal, India, Bangladesh, Brazil and Sudan constitute five countries of the world where more than 90% of visceral leishmaniasis occurs. The aim of this study is to evaluate haematological profile with available clinical data in visceral leishmaniasis patients and to detect LD bodies among them. MATERIALS AND METHODS: It is a hospital based cross sectional study conducted in the Department of Pathology, BPKIHS, Dharan, for the period of one year. LD bodies were calculated in bone marrow aspirate of forty clinically suspected cases by counting the number of parasites per 100 consecutive oil immersion fields. RESULTS: The age ranged from 2-60 years. Pyerxia was the most common sign (100%) followed by splenomegaly (82.5%), hepatomegaly (65%), and pallor (75%). Anemia was present in 90%, leucopenia in 67.5% and thrombocytopenia in 72.5% cases. Bicytopenia and pancytopenia were observed in 40% and 25% cases, respectively. On peripheral examination RBCs were predominantly normocytic normochromic. On bone marrow examination normocellular marrow and megaloblastic features were predominant findings followed by increased plasma cells. Low, moderate and high grade LD bodies were present in 7.5%, 37.5% and 55% of the cases respectively. Hepatomegaly, anemia, neutropenia and lymphocytosis were statistically significant to parasite load (p-value <0.05). CONCLUSIONS: Besides LD bodies in bone marrow aspirates, dyserythroblastic changes and increase plasma cells are common findings in leishmaniasis. Patient from endemic area with positive clinical history and findings should be examined for LD bodies in marrow if dyserythroblastic and increase plasma cell picture is found.  DOI: http://dx.doi.org/10.3126/ijim.v2i2.8320   Int J Infect Microbiol 2013;2(2):39-44

scholarly journals Complement 4 Aids in Prediction of Newly Diagnosed Multiple Myeloma Outcome in Patients

2021 ◽  
Author(s):  
Lihua Zhang ◽  
Xiaosui Ling ◽  
Fan Li ◽  
Tonghua Yang ◽  
Keqian Shi ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Proportional Hazards ◽  
Plasma Cells ◽  
Newly Diagnosed ◽  
Complement C4 ◽  
Cell Percentage ◽  
Bone Marrow Plasma ◽  
Complement 4 ◽  
Poor Outcomes

Abstract Background and aim: A cure for the heterogeneous hematological malignancy Multiple Myeloma (MM) is yet to be developed. To date, the early risk factors associated with poor outcomes in MM have not been fully elucidated. Studies have shown an aberrant complement system in MM patients, but the precise association necessitates elucidation. Therefore, this study scrutinizes the correlation between serum complement level and the disease outcome of MM patients.Materials and methods: A retrospective analysis of 72 MM patients (new diagnosis) with complement C4 and C3 along with common laboratory indicators was done. The Pearson’s χ2 test and the Mann–Whitney U test were done to evaluate categorical or binary variables and inter-group variance, respectively. Kaplan-Meier test and Cox’s proportional hazards regression were employed for quantitation of overall survival (OS) and univariate or multivariate analyses, respectively.Results: The Cox proportional hazard model analysis unveiled the following: platelet≤115.5×10^9/L(HR=5.82，95%CI=2.522-13.436, P<0.001)， complement C4≤0.095g/L(HR=3.642, 95%CI=1.486-8.924,P=0.005), age≥67 years(HR=0.191, 95%CI=0.078-0.47, P<0.001)，bone marrow plasma cell percentage≥30.75% (HR=0.171, 95%CI=0.06-0.482, P=0.001) can be employed as independent predictors of OS. Of these, advanced age, low platelet level, and a high proportion of bone marrow plasma cells have been implicated in poor outcomes in MM patients. Interestingly, a low complement 4 level can function as a new indicator of poor prognosis in MM patients.Conclusion: Low levels of C4 are indicative of a poor outcome in newly diagnosed MM patients.

Should CD138 Immunohistochemistry be Standard Recommended Practice for Bone Marrow Evaluation of Plasma Cell Neoplasms?

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S110-S110
Author(s):  
A Vijayanarayanan ◽  
K Inamdar ◽  
M Menon ◽  
P Kuriakose
Keyword(s):  
Bone Marrow ◽  
Plasma Cell ◽  
Linear Correlation ◽  
Plasma Cells ◽  
Pearson Correlation ◽  
Protein Electrophoresis ◽  
Pearson Correlation Coefficient ◽  
Cell Percentage ◽  
Significant Linear Correlation ◽  
Plasma Cell Neoplasms

Abstract Introduction/Objective Myeloma diagnosis by a pathologist requires 10% plasma cells (PC) or a biopsy proven plasmacytoma in addition to myeloma defining events. PC% &gt; 60% is a biomarker of malignancy under this definition. WHO allows for assesment of plasma cell percentage either by aspirate count or by CD138 immunohistochemistry (IHC). There is lack of consensus on aspirate smear adequacy for PC% estimation. Uneven distribution of plasma cells, hemodilution and/or patchy infiltration can lead to gross underestimation. We compared PC% by aspirate count and CD138 IHC and established corelation with serum protein electrophoresis (SPEP) values. Methods 67 myeloma cases were included after excluding cases with suboptimal or inadequate aspirate smears. Two hematopathologists evaluated the diagnostic marrow (therapy naive) for PC% by aspirate count and CD138 IHC on biopsy/clot section. Corresponding SPEP and Free light chain (FLC) values were obtained. Correlation coefficent was calculated using Pearson correlation coefficient (GraphPad Prism). Results The Ig subtypes included IgG (41/67) and IgA (17/67). 12 cases had available FLC values. Both average and median PC% by CD138 IHC was considerably higher (50%, 52%) compared to aspirate count (29%, 21%). However, PC% by aspirate smear count and CD138 IHC demonstrated a significant linear correlation (r=0.71, p60% by CD138 (and not by aspirate count). Conclusion CD138 IHC based PC% is consistently higher, nevertheless, statistically significant linear corelation is observed between aspirate count PC% and CD138 IHC. A significant linear correlation is observed between CD138 IHC and SPEP (IgG and IgA), however, no such correlation is observed with aspirate count. More cases were diagnosed as myeloma (11%) and higher propotion of cases (35%) had biomarker of malignancy i.e. PC% &gt;60% by CD138 IHC. Based on these findings, we propose estimation of PC% by CD138 immunostain be a recommended standard practice for better clinicopathologic and biologic correlation.

scholarly journals Bone Marrow Plasma Cells Modulate Local Myeloid-Lineage Differentiation via IL-10

2019 ◽  
Vol 10 ◽  
Author(s):  
Lingzhang Meng ◽  
Larissa Nogueira Almeida ◽  
Ann-Katrin Clauder ◽  
Timo Lindemann ◽  
Julia Luther ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Plasma Cells ◽  
Myeloid Lineage ◽  
Lineage Differentiation ◽  
Bone Marrow Plasma

scholarly journals T cell suppression in the bone marrow of visceral leishmaniasis patients: impact of parasite load

2017 ◽  
Vol 191 (3) ◽  
pp. 318-327 ◽  
Author(s):  
P. Kumar ◽  
P. Misra ◽  
C. P. Thakur ◽  
A. Saurabh ◽  
N. Rishi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Visceral Leishmaniasis ◽  
Parasite Load ◽  
T Cell Suppression ◽  
Cell Suppression

scholarly journals Multiple myeloma: circulating lymphocytes that express plasma cell antigens

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 352-356
Author(s):  
GJ Ruiz-Arguelles ◽  
JA Katzmann ◽  
PR Greipp ◽  
NJ Gonchoroff ◽  
JP Garton ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Plasma Cell ◽  
Peripheral Blood ◽  
Plasma Cells ◽  
Peripheral Blood Lymphocytes ◽  
Tumor Burden ◽  
B Cell Differentiation ◽  
Blood Lymphocytes ◽  
Bone Marrow Plasma

The bone marrow and peripheral blood of 14 patients with multiple myeloma were studied with murine monoclonal antibodies that identify antigens on plasma cells (R1–3 and OKT10). Peripheral blood lymphocytes expressing plasma cell antigens were found in six cases. Five of these cases expressed the same antigens that were present on the plasma cells in the bone marrow. Patients that showed such peripheral blood involvement were found to have a larger tumor burden and higher bone marrow plasma cell proliferative activity. In some patients, antigens normally found at earlier stages of B cell differentiation (B1, B2, and J5) were expressed by peripheral blood lymphocytes and/or bone marrow plasma cells.

scholarly journals LONG-LIVED BONE MARROW PLASMA CELLS DURING IMMUNE RESPONSE TO ALPHA (1→3) DEXTRAN

2015 ◽  
Vol 17 (2) ◽  
pp. 127
Author(s):  
I. N. Chernyshova ◽  
M. V. Gavrilova ◽  
L. V. Komarova ◽  
E. V. Sidorova
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Plasma Cells ◽  
Bone Marrow Plasma
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