scholarly journals Initial response of human bone marrow–derived stem cells after contact with ultrahigh-molecular-weight polyethylene (UHMWPE) material: An in vitro study on cell viability and interleukin-6 expression

2018 ◽  
Vol 10 (1) ◽  
pp. 43 ◽  
Author(s):  
IwanB Anwar ◽  
Asep Santoso ◽  
Eko Saputra ◽  
Rifky Ismail ◽  
J Jamari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Molecular Weight ◽  
Bone Marrow ◽  
Cell Viability ◽  
Interleukin 6 ◽  
In Vitro Study ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
Initial Response ◽  
Ultrahigh Molecular Weight

In Vitro Study Bioactivity of HA/UHMWPE Nanocomposites

2007 ◽  
Vol 342-343 ◽  
pp. 701-704
Author(s):  
Li Ming Fang ◽  
Yang Leng ◽  
Ping Gao
Keyword(s):  
Molecular Weight ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
In Vitro Study ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
Octacalcium Phosphate ◽  
Tof Sims ◽  
Ultrahigh Molecular Weight

Bioactivity of hydroxyapatite reinforced ultrahigh molecular weight polyethylene (HA/UHMWPE) nanocomposites with HA volume content of 10~50 % was evaluated by simulated body fluid (SBF) immersion. The effect of HA content on the capability for calcium phosphate (Ca- P) induction was studied. It was found that Ca-P deposition covered the whole surface of the composite with 30 vol. % of HA after immersion for 1 day and the layer grew to around 10 0m thick in one-week immersion, while there was few nucleus formed for composites with HA content lower than 30 vol. % after one-week immersion. The Ca-P structure was identified as octacalcium phosphate (OCP) by SEM, TEM, and ToF-SIMS.

In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells

2017 ◽  
Vol 357 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Long-Wei Hu ◽  
Xiao Wang ◽  
Xin-Qun Jiang ◽  
Li-Qun Xu ◽  
Hong-Ya Pan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelin 1

scholarly journals Porous Ultrahigh Molecular Weight Polyethylene/Functionalized Activated Nanocarbon Composites with Improved Biocompatibility

Materials ◽  
2021 ◽  
Vol 14 (20) ◽  
pp. 6065
Author(s):  
Wangxi Fan ◽  
Xiuqin Fu ◽  
Zefang Li ◽  
Junfei Ou ◽  
Zhou Yang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Contact Angle ◽  
Tensile Strength ◽  
Wear Resistance ◽  
Biomedical Applications ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
In Vitro Experiments ◽  
Ultrahigh Molecular Weight ◽  
Uhmwpe Composite

Ultrahigh molecular weight polyethylene (UHMWPE) materials have been prevalent joint replacement materials for more than 45 years because of their excellent biocompatibility and wear resistance. In this study, functionalized activated nanocarbon (FANC) was prepared by grafting maleic anhydride polyethylene onto acid-treated activated nanocarbon. A novel porous UHMWPE composite was prepared by incorporating the appropriate amount of FANC and pore-forming agents during the hot-pressing process for medical UHMWPE powder. The experimental results showed that the best prepared porous UHMWPE/FANC exhibited appropriate tensile strength, porosity, and excellent hydrophilicity, with a contact angle of 65.9°. In vitro experiments showed that the porous UHMWPE/FANC had excellent biocompatibility, which is due to its porous structure and hydrophilicity caused by FANC. This study demonstrates the potential viability for our porous UHMWPE/FANC to be used as cartilage replacement material for biomedical applications.

scholarly journals Presence of mixed colony-forming cells in long-term hamster bone marrow suspension cultures: response to pokeweed spleen conditioned medium

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 495-502
Author(s):  
CE Eastment ◽  
FW Ruscetti ◽  
E Denholm ◽  
I Katznelson ◽  
E Arnold ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Conditioned Medium ◽  
In Vitro Study ◽  
Suspension Cultures ◽  
Rapid Expansion ◽  
Pokeweed Mitogen ◽  
Hematopoietic Stem

In contrast to the murine system, long-term hamster bone marrow suspension cultures maintain proliferation of both pluripotent and committed stem cells in the absence of an adherent layer and without addition of exogenous factors, such as hydrocortisone. Addition of pokeweed-mitogen-stimulated hamster spleen conditioned medium (SCM) to these long-term suspension cultures produces an increase in the number of mixed colonies assayed in soft-agar, These mixed colonies, which contained four cell lineages--granulocytic, erythroid, megakaryocytic, and macrophage--could be generated from cells grown in suspension for over 6 mo. Addition of SCM also induces an initial rapid expansion of the myeloid compartment, and this expansion results in 70% of the cells being terminally differentiated granulocytes. In contrast, addition of SCM to hamster bone marrow cultures containing both adherent cells and hematopoietic stem cells produced no change in the number of mixed colonies generated in the culture. This system allows the in vitro study of the process of stem cell proliferation and differentiation and also provides a means to examine the relationship of adherent and supernatant bone marrow populations.

scholarly journals Hyperglycemia induces osteogenic differentiation of bone marrow derived stem cells: an in vitro study

2019 ◽  
Vol 08 (01) ◽  
pp. 1 ◽  
Author(s):  
A. Giai Via ◽  
M.B. McCarthy ◽  
M. Francke ◽  
F. Oliva ◽  
A.D. Mazzocca ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals An in vitro Evaluation of the Cytotoxicity of Varying Concentrations of Sodium Hypochlorite on Human Mesenchymal Stem Cells

2014 ◽  
Vol 15 (4) ◽  
pp. 473-481 ◽  
Author(s):  
Zeeshan H Ahmad ◽  
Sarah M Alkahtany ◽  
Sukumaran Anil
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Sodium Hypochlorite ◽  
Human Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

ABSTRACT Aim To evaluate and compare the cytotoxicity of various concentrations of sodium hypochlorite on immortalized human bone marrow mesenchymal stem cells (MSCs). Materials and methods The 5.25 percent sodium hypochlorite (NaOCl) at concentrations of 0.5, 0.1, 0.025, 0.0125, and 0.005 mg/ml were used to assess the cytotoxic effect on MSCs. Immortalized human bone marrow mesenchymal stem cells (hTERT-MSCs) were exposed to NaOCl at 5 different concentrations. Cell viability was assessed by 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) and alamarBlue assays. The cell morphology changes were assessed with scanning electron microscopy (SEM) after exposure to 2, 4, and 24 hour incubation. The ethidium bromide/acridine orange (EB/ AO) fluorescent stain was applied to the cells in the 8-chamber slides after they were incubated with the testing agents for 2 and 4 hours to detect live and dead cells. The observations were quantitatively and qualitatively analyzed. Results The cell viability study using MTT assay and AB assay showed significant reduction with varying concentration at 2 and 4 hours incubation period. The cell viability decreased with the higher percentage of NaOCl. The exposure time also revealed an inverse relation to the cell viability. The SEM analysis showed reduction in the number of cells and morphological alterations with 0.5 mg/ml at 2 and 4 hours compared to 0.025 mg/ml NaOCl. Destruction of the cells with structural alterations and lysis was evident under fluorescence microscope when the cells were exposed to 0.5 mg/ml NaOCl. Conclusion Within the limitations of this in vitro study it can be concluded that NaOCl is toxic to the human bone marrow MSCs. The cell lysis was evident with higher concentration of sodium hypochlorite. From the observations, it can be concluded that a lower concentration of NaOCl may be used as endodontic irrigant due to its cytotoxic properties. Further studies are man datory to evolve a consensus on the optimal concentration of sodium hypochlorite to be used as endodontic irrigant. How to cite this article Alkahtani A, Alkahtany SM, Anil S. An in vitro Evaluation of the Cytotoxicity of Varying Concentrations of Sodium Hypochlorite on Human Mesenchymal Stem Cells. J Contemp Dent Pract 2014;15(4):473-481.

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