scholarly journals Determining frequency of genes of CTX-M and CTX-M-15 of producing Enterobacteriaceae of isolated extended-spectrum beta-lactamases from clinical samples of patients referred to training hospitals of Medical Sciences University, Khorramabad, Iran

2017 ◽  
Vol 7 (2) ◽  
pp. 60 ◽  
Author(s):  
Azam Rezvani-Rad ◽  
SeyedMojtaba Mosavian
Keyword(s):  
Clinical Samples ◽  
Medical Sciences ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases

scholarly journals Antimicrobial Resistance Patterns in Clinical Isolates of Enterobacteriaceae from a Tertiary Care Hospital, Kathmandu, Nepal

2018 ◽  
Vol 1 (2) ◽  
pp. 74-78
Author(s):  
Ram Prasad Adhikari ◽  
Subha Shrestha ◽  
Junu Richhinbung Rai ◽  
Ritu Amatya
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
College Teaching ◽  
Clinical Isolates ◽  
High Rate ◽  
Clinical Samples ◽  
Care Hospital ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases

Introduction: Multidrug resistance among Enterobacteriaceae is in increasing trend these days. The objective of this study was to determine the antibiogram of clinical isolates of Enterobacteriaceae with special reference to multidrug resistance and  extended spectrum beta-lactamases production.Materials and Methods: A descriptive cross sectional study was conducted over a period of six months (February -July, 2017) in the microbiology laboratory of Nepal Medical College Teaching Hospital, Kathmandu, Nepal. A total of 936 bacterial isolates of Enterobacteriaceae from clinical specimens were processed for antimicrobial susceptibility testing and screened for multidrug resistance. ESBL production was detected among potential isolates by combination disk diffusion test.Results: The rate of multidrug resistance and extended spectrum beta-lactamases production was 54.2% and 23.8% respectively. Of the total ESBL producers 92.4% were multidrug resistance. The rate of multidrug resistance and extended spectrum beta-lactamases production were higher in organisms isolated from clinical samples collected from inpatients. High rate of multidrug resistance and extended spectrum beta-lactamases production was seen in E. coli (54.4% & 27.7%), Klebsiella spp. (67.1% & 28.2%) and Citrobacter spp. (70.3% & 10.9%). The antimicrobial resistance rate was highest against ampicillin (76.7%) followed by cefixime (54. 0%), ceftazidime (51.5%), ceftriaxone (51.0%), cotrimoxazole (48.7%), ciprofloxacin (43.9%) and ofloxacin (41.1%).Conclusions:  Multidrug resistance is common among Enterobacteriaceae. These bacteria have high rate of resistance against commonly used groups of antibiotics like cephalosporins and quinolones. Continuous monitoring, surveillance of antimicrobial resistance, proper infection control and practices are important to combat with these issues.

scholarly journals Antimicrobial Resistance Patterns and Extended Spectrum Beta-lactamases Producing by Proteus mirabilis Isolated from Different Sources

2017 ◽  
Vol 28 (1) ◽  
pp. 47
Author(s):  
Ali S. Kadhim
Keyword(s):  
Proteus Mirabilis ◽  
Clinical Samples ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Antibiotic Susceptibility Test ◽  
Resistance Patterns ◽  
Extended Spectrum Beta Lactamases ◽  
Resistance Rates ◽  
Different Sources ◽  
Animal Isolates

A total of 801 samples included 179 clinical samples and 622 animal samples were collected from Baghdad province. All samples were cultured on blood agar and MacConkey agar plates to isolate proteus mirabilis bacteria. Results showed that rate isolates of P. mirabilis from clinical and animal samples were 25.89% (51/179) and 5.95% (37/622) respectively. Antibiotic susceptibility test showed that lowest resistance rates for clinical P. mirabilis isolates were 3.9% for ciprofloxacin, 7.8% for norfloxacin, 9.8% for imipenem, 13.7% for levofloxacin and 15.7% for cefotaxime, and highest resistance rates were 82.4% for cefepime, 78.4% for piperacillin, 56.9% for ceftazidime and 54.9% for cefoxitin. In regards to animal isolates, they were 100% sensitive to cefoxitin and 100% resistance to piperacillin. Their resistance rates were 2.7% to amikacin, 5.4% to ciprofloxacin and 8.1% to cefepime, imipenem and levofloxacin. The results revealed that all P. mirabilis isolates were 100% multidrug resistance for 2-8 antibiotics. Extended spectrum β-lactamases produced were detected in 52.94% of clinical P. mirabilis isolates and in 48.65% of animal isolates.

scholarly journals Extended Spectrum Beta-Lactamases Producers among Gram-negative Bacteria from Clinical and Environmental Sources in Two Tertiary Hospitals in Makurdi, Nigeria

2020 ◽  
pp. 42-53
Author(s):  
Florence Bose Omoregbe ◽  
Obasola Ezekiel Fagade
Keyword(s):  
Clinical Isolates ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Multiple Drug ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Negative ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases

Extended Spectrum Beta-Lactamases (ESBLs) enzymes are produced by many Gram-negative bacteria to nearly all antibiotics. Clinical bacteria isolates were obtained from various clinical samples from the two healthcare facilities. Sewer wastewater and sediments were also collected from both hospitals using standard sampling techniques and bacteria isolated using pour plate technique. Multiple drug resistant patterns were determined using disc diffusion technique. Antibiotics sensitivity of the isolates was verified using disc diffusion method. Detection of ESBL producing bacteria was done using double disc synergy test. Data obtained were analysed using descriptive statistics. Clinical bacteria (403) were obtained, out of which 299 were confirmed Gram-negative, 218 from Federal Medical Centre (FMC) and 81 from Benue State Teaching Hospital (BSUTH). Thirty-nine Gram-negative bacteria were also isolated from the environmental samples. The ESBL producers in clinical isolates were 69 (FMC) and 42 (BSUTH) while in environmental isolates they were eight and four respectively. Out of a total of 338 environmental and clinical isolates from the two hospitals, 216 shown resistance/intermediate resistance to Ceftazidime. Of these 216, 123(36.39%) were positive for ESBLs production.- space From FMC, ESBLs producing bacteria are highly sensitive to imipenem with sensitivity frequency of 62 while they were highly resistant to ceftazidime with a frequency of 52. Age groups 0-5 had the highest percentage distribution of 21.43% and 10.14% from BSUTH and FMC respectively. ESBLs producing bacteria showed multidrug resistance.

scholarly journals Phenotypic detection of Extended Spectrum Beta-Lactamases and Amp C Beta- Lactamases among nosocomial isolates in a tertiary care hospital

2013 ◽  
Vol 12 (4) ◽  
pp. 378-384
Author(s):  
V Vijayvergia ◽  
AK Sahni ◽  
M Lall ◽  
K Vijay ◽  
ID Khan
Keyword(s):  
Tertiary Care ◽  
Clinical Samples ◽  
Simultaneous Occurrence ◽  
Kappa Statistics ◽  
Care Hospital ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases ◽  
Disk Test

Background & objectives: Extended-Spectrum Beta-Lactamases (ESBLs) is an important resistance mechanism in Enterobacteriaceae infections. Lack of standard guidelines from Clinical Laboratory Standards Institute (CLSI) for Amp C beta-lactamase detection poses a problem. This study was undertaken to detect ESBLs by phenotypic tests and Amp C beta-lactamase by inhibitor based method. Material and Methods: 200 consecutive non-repetitive isolates of E.coli, Klebsiella and Proteus from clinical samples were screened for ESBLs as per CLSI guidelines and confirmed by PCDT, DDST and E-tests (AB Biodisk, Biomerieux). Amp C beta lactamases were screened by cefoxitin resistance and confirmed by inhibitor (Cloxacillin) based method. Simultaneous occurrence of Amp C and ESBLs was detected by combined disk test (Neo-Sensitabs and Diatabs). Descriptive and Kappa statistics were used. Results: Out of 200 isolates studied, 131 were initially screened as ESBL producers and later 114 (57%) were confirmed by phenotypic methods. E-Test was found most sensitive phenotypic test as compared to PCDT and DDST. 13 strains resistant to cefoxitin (30?g) were found to be pure Amp C producers. Combined disk test detected 36 to be ESBL and Amp C co-producers. Surprisingly, six isolates found sensitive to cefoxitin disk were confirmed as Amp C producers by cloxacillin disk inhibition test. Conclusion: 57% ESBLs and 27.5% Amp C producers were isolated from nosocomial pathogens showing significant resistance to 3rd generation cephalosporins. Phenotypic confirmation by E-test, PCDT & DDST were useful for ESBL identification and for detection of Amp C, cloxacillin was found to be an effective inhibitor. DOI: http://dx.doi.org/10.3329/bjms.v12i4.13309 Bangladesh Journal of Medical Science Vol. 12 No. 04 October ’13 Page 378-384

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