Gas chromatography-mass spectrometry elucidation and antipsoriatic activity on developed herbal formulations from Carissa congesta, Catharanthus roseus, Annona squamosa, and Polyalthia longifolia Plant Extracts

Catharanthus roseus (periwinkle) is a medicinal plant commonly known for its wide biological activity. In many countries different parts of this plant are used for the treatment of diabetes, hypertension and for menstrual regulation. Due to the ability of production of alkaloids, which can be applied in cancer therapy, is still extensively investigated. Two, the mostvaluable alkaloids (vincristine and vinblastine) are present in C. roseus in very low concentrations. Micropropagation is promising technique used to enhance the level of important secondary metabolites. The main objective of present study was alkaloids extraction from plants cultivated conventionally and derived from in vitro cultures. In this order the aerial parts of periwinkle were extracted with 96% ethanol at room temperature (method I) and heated with 96% ethanol at 55 °C for 90 minutes (method II). The obtained mixtures of different indole alkaloids were analyzed by gas chromatography – mass spectrometry (GC-MS). Analysis revealed the presence of 15 alkaloids, among which vindoline,vindorosine,isovindolinineand ajmalicine were the most abundant. The obtained results indicated that the propagation method had a significant effect on the percentage content of alkaloids in C. roseus herb. Plants derived from in vitro cultures were richer in vindorosine and vindoline, while conventionally cultivated – in tetrahydroalstonine and ajmalicine. Moreover, in case of isovindolinine,vindolinine and ajmalicine, extraction at 55 °C was more effective, while for pericyclivine – maceration at room temperature.Interestingly, the pericyclivine was not detected in the mixture of alkaloids obtained from periwinkle herb by the extraction at55 °C.

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Quantification of Arbutin in Plant Extracts by Stable Isotope Dilution Gas Chromatography–Mass Spectrometry

Chromatographia ◽

10.1007/s10337-017-3461-5 ◽

2018 ◽

Vol 81 (3) ◽

pp. 533-538 ◽

Cited By ~ 4

Author(s):

Kyoung Bok Lee ◽

Jaehyuk Choi ◽

Soon Kil Ahn ◽

Jong-Kuk Na ◽

Krishna Kumar Shrestha ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Stable Isotope ◽

Plant Extracts ◽

Isotope Dilution ◽

Gas Chromatography Mass Spectrometry ◽

Stable Isotope Dilution ◽

Chromatography Mass Spectrometry

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Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from Catharanthus roseus (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha.44.1.10127 ◽

2016 ◽

Vol 44 (1) ◽

Author(s):

Aneta WESOŁOWSKA ◽

Monika GRZESZCZUK ◽

Janusz WILAS ◽

Danuta KULPA

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Catharanthus Roseus ◽

Indole Alkaloids ◽

In Vitro Cultures ◽

Gas Chromatography Mass Spectrometry ◽

Chromatography Mass Spectrometry ◽

Ms Analysis

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Antimony speciation in freshwater plant extracts by using hydride generation–gas chromatography–mass spectrometry

The Analyst ◽

10.1039/an9962100223 ◽

1996 ◽

Vol 121 (2) ◽

pp. 223-228 ◽

Cited By ~ 62

Author(s):

Mattew Dodd ◽

Spiros A. Pergantis ◽

William R. Cullen ◽

Hao Li ◽

Guenter K. Eigendorf ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Plant Extracts ◽

Hydride Generation ◽

Gas Chromatography Mass Spectrometry ◽

Chromatography Mass Spectrometry ◽

Antimony Speciation

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COMPREHENSIVE INVITRO EVALUATION OF PHARMACOLOGICAL ACTIVITIES OF SELECTED PLANT EXTRACTS AND GC-MS PROFILING OF FLACOURTIA JANGOMAS FLOWER EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i5.17419 ◽

2017 ◽

Vol 10 (5) ◽

pp. 237

Author(s):

Sangeetha Annie George

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Cytotoxic Activity ◽

Plant Extracts ◽

Mtt Assay ◽

Antiproliferative Activity ◽

Gas Chromatography Mass Spectrometry ◽

Flower Extract ◽

Chromatography Mass Spectrometry ◽

Ic50 Values

Objective: Continued exploration and bio-evaluation of plants hold good as there are an increasing demand and recognition of natural products in disease management. The aim of this study was to investigate the antioxidant, cytotoxic, and antiproliferative activity of methanolic extracts of different parts of six plants. Six plants - Couroupita guianensis, Flacourtia jangomas, Lucuma nervosa, Euphorbia milii, Acalypha hispida, and Hydnocarpus pentandra - were chosen for this study.Methods: The plant parts were extracted with methanol and screened for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging abilities. The cytotoxic activity of the extracts was investigated on SCC9 and Calu6 through MTT assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The flower extract of F. jangomas was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A twoway ANOVA was done to estimate the effect of the extract between samples remembered at p<0.05 level.Results: Among all the plant extracts, the extract of F. jangomas (flower) showed significant antioxidant potential with IC50 values of 11.16±0.54 μg/mland 12.34±0.37 μg/ml for DPPH and ABTS assays, respectively. GC-MS analysis of the extract revealed the presence of 21 phytocompounds. MTT assay revealed that this extract had promising cytotoxic activity against the two cancer cell lines, Calu6 and SCC9 with IC50 values of 43.57±0.04 μg/ml and 53.42±0.15 μg/ml, respectively. The extract treatment caused significant arrest in G2M phase of cell cycle.Conclusion: F. jangomas flower extract displayed significant antioxidant and antiproliferative activity and can be considered as a potential source ofanticancer compounds.Keywords: Antioxidants, MTT assay, Gas chromatography-mass spectrometry, Calu6, SCC9, Flacourtia jangomas.

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