Identification of gibberellins in crude plant extracts by combined gas chromatography-mass spectrometry

Objective: Continued exploration and bio-evaluation of plants hold good as there are an increasing demand and recognition of natural products in disease management. The aim of this study was to investigate the antioxidant, cytotoxic, and antiproliferative activity of methanolic extracts of different parts of six plants. Six plants - Couroupita guianensis, Flacourtia jangomas, Lucuma nervosa, Euphorbia milii, Acalypha hispida, and Hydnocarpus pentandra - were chosen for this study.Methods: The plant parts were extracted with methanol and screened for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging abilities. The cytotoxic activity of the extracts was investigated on SCC9 and Calu6 through MTT assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The flower extract of F. jangomas was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A twoway ANOVA was done to estimate the effect of the extract between samples remembered at p<0.05 level.Results: Among all the plant extracts, the extract of F. jangomas (flower) showed significant antioxidant potential with IC50 values of 11.16±0.54 μg/mland 12.34±0.37 μg/ml for DPPH and ABTS assays, respectively. GC-MS analysis of the extract revealed the presence of 21 phytocompounds. MTT assay revealed that this extract had promising cytotoxic activity against the two cancer cell lines, Calu6 and SCC9 with IC50 values of 43.57±0.04 μg/ml and 53.42±0.15 μg/ml, respectively. The extract treatment caused significant arrest in G2M phase of cell cycle.Conclusion: F. jangomas flower extract displayed significant antioxidant and antiproliferative activity and can be considered as a potential source ofanticancer compounds.Keywords: Antioxidants, MTT assay, Gas chromatography-mass spectrometry, Calu6, SCC9, Flacourtia jangomas.

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Phytochemical Screening of Plant Extracts and GC-MS Analysis of n-Hexane Extract of the Leaves of Cassia alata Linn

The Journal of Phytopharmacology ◽

10.31254/phyto.2020.9509 ◽

2020 ◽

Vol 9 (5) ◽

pp. 342-347

Author(s):

Koushik Saha ◽

◽

Rezwana Zaman Proma ◽

Nasima Khan ◽

◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Plant Extracts ◽

Hexane Extract ◽

Biologically Active ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Screening ◽

Leaf Extracts ◽

Cassia Alata ◽

Chromatography Mass Spectrometry

The purpose of this investigation was to undergo the preliminary phytochemical screening of the plant extracts and identify the presence of pharmacologically bioactive constituents in the leaves of Cassia alata by using gas chromatography-mass spectrometry (GC-MS) technique. The preliminary phytochemical screening of different leaf extracts of Cassia alata revealed the presence of various phytochemical compounds such as terpenoids, steroids, flavonoids, phenolic compounds, quinones, carbohydrates, tannins and alkaloids. Qualitative and quantitative determination of different biologically active compounds from the crude n-hexane extract using gas chromatography-mass spectrometry disclosed 20 compounds with varying amounts where main components were identified as palmitic acid (26.65%), stearic acid (14.27%), (E)-9-octadecadienoic acid (11.40%), erucylamide (8.34%), 1,19-eicosadiene (5.15%), stigmasterol (4.68%), linoleic acid (4.06%), vitamin E (3.97%), methyl palmitate (3.93%) and methyl 11-octadecenoate (3.32%). The compounds identified through this investigation may be responsible for any of the pharmacological properties of Cassia alata and could be of considerable interests for the development of new drug leads.

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