scholarly journals COMPREHENSIVE INVITRO EVALUATION OF PHARMACOLOGICAL ACTIVITIES OF SELECTED PLANT EXTRACTS AND GC-MS PROFILING OF FLACOURTIA JANGOMAS FLOWER EXTRACT

2017 ◽  
Vol 10 (5) ◽  
pp. 237
Author(s):  
Sangeetha Annie George
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Plant Extracts ◽  
Mtt Assay ◽  
Antiproliferative Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Flower Extract ◽  
Chromatography Mass Spectrometry ◽  
Ic50 Values

Objective: Continued exploration and bio-evaluation of plants hold good as there are an increasing demand and recognition of natural products in disease management. The aim of this study was to investigate the antioxidant, cytotoxic, and antiproliferative activity of methanolic extracts of different parts of six plants. Six plants - Couroupita guianensis, Flacourtia jangomas, Lucuma nervosa, Euphorbia milii, Acalypha hispida, and Hydnocarpus pentandra - were chosen for this study.Methods: The plant parts were extracted with methanol and screened for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging abilities. The cytotoxic activity of the extracts was investigated on SCC9 and Calu6 through MTT assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The flower extract of F. jangomas was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A twoway ANOVA was done to estimate the effect of the extract between samples remembered at p<0.05 level.Results: Among all the plant extracts, the extract of F. jangomas (flower) showed significant antioxidant potential with IC50 values of 11.16±0.54 μg/mland 12.34±0.37 μg/ml for DPPH and ABTS assays, respectively. GC-MS analysis of the extract revealed the presence of 21 phytocompounds. MTT assay revealed that this extract had promising cytotoxic activity against the two cancer cell lines, Calu6 and SCC9 with IC50 values of 43.57±0.04 μg/ml and 53.42±0.15 μg/ml, respectively. The extract treatment caused significant arrest in G2M phase of cell cycle.Conclusion: F. jangomas flower extract displayed significant antioxidant and antiproliferative activity and can be considered as a potential source ofanticancer compounds.Keywords: Antioxidants, MTT assay, Gas chromatography-mass spectrometry, Calu6, SCC9, Flacourtia jangomas.

scholarly journals SCREENING OF BIOPROTECTIVE PROPERTIES OF VARIOUS PLANT EXTRACTS AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING OF ADENANTHERA PAVONINA STEM EXTRACT

2017 ◽  
Vol 10 (7) ◽  
pp. 188 ◽  
Author(s):  
Shubha Bhadran ◽  
Sangeetha Annie George ◽  
Sudhakar Malla ◽  
Harini Bp
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Mtt Assay ◽  
Antiproliferative Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Potential Candidate ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Stem Extract

Objective: The search for various phytotherapeutic compounds is on rise due to a complex multifactorial phenomenon called drug resistance. The present study investigates the cytotoxic, antioxidant, and antiproliferative potential of methanolic extracts of Clitorea ternatea, Averrhoa bilimbi, Phyllanthus acidus, Tecoma stans, Curcuma aromatica, Anethum graveolens, Adhatoda vasica, Markhamia lutea, Spathodea companulata, and Adenanthera pavonina.Methods: The plant parts were extracted with methanol and screened for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging abilities. The cytotoxic activity of the extracts was investigated on HeLa and HCT116 cells through 3-(4,5-dimethylthiazol2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The stem extract of A. pavonina was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A two-way ANOVA was done to estimate the effect of the extract between samples remembered at p<0.05 level.Results: Among all the studied samples, the extract of A. pavonina (stem) showed significant scavenging activity of 70.23% and 76.32% of scavenging compared to 74.58 % and 81.13% of that of reference standard in ABTS and DPPH assay, respectively. GC-MS analysis of the extract revealed the presence of 17 phytocompounds. MTT assay revealed that this extract (SB19) had promising cytotoxic activity against the two cancer cell lines, HCT116 and HeLa with inhibitory concentration 50% IC50 values of 25.86±0.21 μg/ml and 39.89±0.11 μg/ml, respectively. The extract treatment caused significant arrest in G2M phase of cell cycle.Conclusion: A. pavonina (stem) extract displayed significant antioxidant and antiproliferative activity and can be considered as a potential candidate drug for anticancer studies.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

Quantification of Arbutin in Plant Extracts by Stable Isotope Dilution Gas Chromatography–Mass Spectrometry

2018 ◽  
Vol 81 (3) ◽  
pp. 533-538 ◽  
Author(s):  
Kyoung Bok Lee ◽  
Jaehyuk Choi ◽  
Soon Kil Ahn ◽  
Jong-Kuk Na ◽  
Krishna Kumar Shrestha ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Plant Extracts ◽  
Isotope Dilution ◽  
Gas Chromatography Mass Spectrometry ◽  
Stable Isotope Dilution ◽  
Chromatography Mass Spectrometry

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

Antimony speciation in freshwater plant extracts by using hydride generation–gas chromatography–mass spectrometry

The Analyst ◽  
1996 ◽  
Vol 121 (2) ◽  
pp. 223-228 ◽  
Author(s):  
Mattew Dodd ◽  
Spiros A. Pergantis ◽  
William R. Cullen ◽  
Hao Li ◽  
Guenter K. Eigendorf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Plant Extracts ◽  
Hydride Generation ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Antimony Speciation

Phytochemical Screening of Plant Extracts and GC-MS Analysis of n-Hexane Extract of the Leaves of Cassia alata Linn

2020 ◽  
Vol 9 (5) ◽  
pp. 342-347
Author(s):  
Koushik Saha ◽  
◽  
Rezwana Zaman Proma ◽  
Nasima Khan ◽  
◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Plant Extracts ◽  
Hexane Extract ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Cassia Alata ◽  
Chromatography Mass Spectrometry

The purpose of this investigation was to undergo the preliminary phytochemical screening of the plant extracts and identify the presence of pharmacologically bioactive constituents in the leaves of Cassia alata by using gas chromatography-mass spectrometry (GC-MS) technique. The preliminary phytochemical screening of different leaf extracts of Cassia alata revealed the presence of various phytochemical compounds such as terpenoids, steroids, flavonoids, phenolic compounds, quinones, carbohydrates, tannins and alkaloids. Qualitative and quantitative determination of different biologically active compounds from the crude n-hexane extract using gas chromatography-mass spectrometry disclosed 20 compounds with varying amounts where main components were identified as palmitic acid (26.65%), stearic acid (14.27%), (E)-9-octadecadienoic acid (11.40%), erucylamide (8.34%), 1,19-eicosadiene (5.15%), stigmasterol (4.68%), linoleic acid (4.06%), vitamin E (3.97%), methyl palmitate (3.93%) and methyl 11-octadecenoate (3.32%). The compounds identified through this investigation may be responsible for any of the pharmacological properties of Cassia alata and could be of considerable interests for the development of new drug leads.

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