scholarly journals Combination of Immunohistochemistry and Ploidy Analysis to Assist Histopathological Diagnosis of Molar Diseases

2008 ◽  
Vol 1 ◽  
pp. CPath.S601 ◽  
Author(s):  
M.C. Osterheld ◽  
L. Caron ◽  
P. Chaubert ◽  
K. Meagher-Villemure
Keyword(s):  
Histological Analysis ◽  
Protein A ◽  
Image Cytometry ◽  
First Trimester ◽  
Internal Controls ◽  
Histopathological Diagnosis ◽  
Complete Mole ◽  
Products Of Conception ◽  
Partial Mole ◽  
Hydropic Abortion

Background Differential diagnosis between hydropic abortion, partial mole and complete mole is still a challenge for pathologists but really important for patient management. Material and Method In this study, we have evaluated 111 products of conception from the first trimester. Histological analysis was made according to the main diagnostic histopathological features described in the literature and the cases were categorized in hydropic abortus (HA), partial mole (PM) and complete mole (CM). Immunohistochemistry was performed using monoclonal antibody against p57kip protein a putative paternally imprinted inhibitor gene and DNA ploidy was analysed in all cases by image cytometry. Results All 23 HAs presented a diploid DNA content and were p57kip2 positive. From the 28 CMs, 12 cases (43%) were diploid and 16 cases (57%) were tetraploid but no expression of p57kip2 was found with positive internal controls. From the 60 PMs, 58 cases were positive for p57kip2 expression and 53 cases (88%) were triploid, 6 cases (10%) tetraploid and 1 case (2%) diploid. Conclusion This study on 111 cases of early pregnancies confirms the usefulness of immunohistochemistry and cytometry but demonstrates the importance of the combination of both techniques to assist histology for the best reliable diagnosis.

scholarly journals Histopathological examination of tissues following a surgical uterine evacuation in the first trimester bleeding: clinical relevance

2020 ◽  
Vol 10 (3) ◽  
pp. 91-94
Author(s):  
Bandana Khanal ◽  
Basant Sharma ◽  
Renuka Tamrakar ◽  
Prekshya Singh
Keyword(s):  
Clinical Relevance ◽  
Histopathological Examination ◽  
First Trimester ◽  
Gestational Trophoblastic Disease ◽  
Complete Mole ◽  
Products Of Conception ◽  
Partial Mole ◽  
History Of

Background: There is no general consensus about the role of routine HPE of the obtained tissue at the time of uterine evacuation. However, it is understood clinically that it is of utmost importance to prove the presence of intrauterine gestation and to exclude gestational trophoblastic disease in the form of partial or complete mole. This study aimed to assess the role of histopathology in cases of first trimester miscarriages and to determine clinical relevance of histopathological examination following surgical evacuation. Methods: This was a retrospective study of collected data over 12 months (January 2018- De­cember 2018) in an agency for reproductive health setup with predefined inclusion criteria. We included 60 consecutive patients attended with history of first trimester bleeding. Patient’s record and the histopathological examination report of products of conception following uterine evacu­ation were observed. The data was analyzed descriptive statistics including percentage, stander deviation, mean, and range in Microsoft Excel software. Results: Missed abortion was the most common type and constituted 55% of the studied group. The histopathological reports confirmed the pregnancy in all patients and revealed partial mole in 6(10%) patients and complete mole in 2(3.33%) patients. Conclusions: Histopathological examination of products of conception detects under diagnosed molar pregnancies that necessitates special follow up and is a key step to do further management.

Histopathological Diagnosis of Partial and Complete Hydatidiform Mole in the First Trimester of Pregnancy

2003 ◽  
Vol 6 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Neil J. Sebire ◽  
Rosemary A. Fisher ◽  
Helene C. Rees
Keyword(s):  
Early Pregnancy ◽  
Hydatidiform Mole ◽  
First Trimester ◽  
Histopathological Diagnosis ◽  
Molar Pregnancy ◽  
Diagnostic Problem ◽  
Complete Hydatidiform Mole ◽  
Products Of Conception ◽  
Genetic Abnormalities ◽  
First Trimester Of Pregnancy

The diagnosis of molar pregnancy is a continuing diagnostic problem for many practicing histopathologists who are required to examine specimens of products of conception, particularly since changes in gynecological management in recent years have resulted in uterine evacuation at earlier gestations. The aim of this review is to provide practical, up-to-date, diagnostically useful information regarding the histological diagnosis of molar disease in early pregnancy. Pathophysiological issues relevant to molar pregnancies, such as genetic abnormalities, will be briefly summarized, but nonhistopathological aspects of molar disease will not be covered in detail in this review.

scholarly journals Role of the Immunohistochemical Marker (Ki67) in Diagnosis and Classification of Hydatidiform Mole

2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Hasan Abu Deka FF ◽  
Abd Ali Al Saeng ZH ◽  
Khalid Almukhtar Z
Keyword(s):  
Stromal Cells ◽  
Hydatidiform Mole ◽  
Labeling Index ◽  
Statistical Relationship ◽  
Ki 67 ◽  
Cross Sectional ◽  
Complete Mole ◽  
Partial Mole ◽  
Hydropic Abortion

Introduction: Since the hallmark of gestational trophoblastic disease is trophoblastic proliferation, Ki67 is regarded as the best marker in studying hydatidiform mole.This study was conducted to evaluate the role of this proliferative marker in distinguishing among hydropic abortion, partial and complete hydatidiform mole. Materials and methods: This is a cross sectional study involving the application of Ki67 on a total of 90 histological samples of curetting materials from molar (partial and complete mole) and non molar hydropic abortion belong to Iraqi females, so three study groups were created. Immunohistochemical expression in villous cytotrophoblasts, syncytiotrophoblasts and stromal cells were recorded separately by three independent observers and the results were correlated statically. Results: The mean number of stained nuclei of villous cytotrophoblasts and stromal cells was the highest in complete mole and the lowest in non molar hydropic abortion. There is a significant statistical relationship regarding Ki67 labeling index in villous cytotrophoblasts between partial moles and hydropic abortion, complete mole and partial moles, hydropic abortion and complete mole. Regarding Ki67 labelling index in villous stromal cells, a significant statistical relationship achieved when the correlation done between partial mole and hydropic abortions, hydropic abortion and complete mole, while a non significant statistical relationship was achieved if the correlation done between partial and complete mole. All villous syncytiotrophoblasts showed negative results. Conclusion: Ki-67 labeling index in villous cytotrophblastic cells are useful in separating between partial moles and hydropic abortion, partial mole and complete mole, hydropic abortion and complete mole. While Ki-67 labeling index in villous stromal cells is only useful in separating between partial moles and hydropic abortion, hydropic abortion and complete mole.

scholarly journals Clinical Usefulness of Immunohistochemical Staining of p57kip2for the Differential Diagnosis of Complete Mole

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Shigeru Sasaki ◽  
Yasushi Sasaki ◽  
Toshiaki Kunimura ◽  
Akihiko Sekizawa ◽  
Yoshihiro Kojima ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Immunohistochemical Staining ◽  
Dna Analysis ◽  
Single Agent ◽  
Positive Staining ◽  
Nuclear Staining ◽  
Complete Mole ◽  
Partial Mole ◽  
Hydropic Abortion

Objective. Can polymer-based immunohistochemical staining ofp57kip2replace DNA analysis as an inexpensive means of differentiating complete mole from partial mole or hydropic abortion?Methods and Materials. Original paraffin-embedded tissue blocks from 14 equivocal cases were turned over to our laboratory and examined by immunohistochemical staining ofp57kip2.Results. Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining. The remaining 10 cases showed apparently positive staining in cytotrophoblasts and villous stromal cells. Without DNA analysis we are able to clearly differentiate the 4 cases of complete mole among the 14 equivocal cases. During follow-up, secondary low-risk gestational trophoblastic neoplasia (GTN) developed in 1 of the 4 cases of complete mole: the GTN was treated by single-agent chemotherapy. No subsequent changes were observed during follow-up in the other cases.Conclusion. Polymer-based immunohistochemical staining ofp57kip2(paternally imprinted gene, expressed from maternal allele) is a very effective method that can be used to differentiate androgenetic complete mole from partial mole and hydropic abortion. We might be able to avoid the cost of DNA analysis.

scholarly journals Histopathological Analysis of Endometrial Biopsy in Gandaki Medical College Teaching Hospital, Pokhara, Nepal

2019 ◽  
Vol 12 (1) ◽  
pp. 25-30
Author(s):  
B Parajuli ◽  
G Pun ◽  
S Ranabhat ◽  
S Poudel
Keyword(s):  
Histopathological Examination ◽  
Hydatidiform Mole ◽  
College Teaching ◽  
Histopathological Diagnosis ◽  
Age Group ◽  
Medical College ◽  
Dilatation And Curettage ◽  
Partial Mole ◽  
Proliferative Endometrium ◽  
The Common

Objective: To study the spectrum of histopathological diagnosis of endometrial lesions and their distribution according to age. Methods: All the endometrium samples obtained by the procedure of dilatation and curettage and hysterectomy sent for histopathological examination at Pathology Department of Gandaki Medical College Teaching Hospital, Pokhara, Nepal. The study duration was total 12 months ranging from July 2016 to June 2017. All the endometrial samples were processed, sectioned at 4 - 6 μm and stained with routine H & E stain. Patient’s data including age, sex, procedure of the biopsy taken and histopathological diagnosis were noted. A pathologist, using Olympus microscope, reported the slides. Cases were reviewed by a second pathologist whenever necessary. Results: A total of 128 cases were studied. The most common histopathological diagnosis was proliferative endometrium (28.9%) followed by disorder proliferative endometrium (15.65%). Most of the patients were in age group 36 - 45 years comprising 32.03%. Hydatidiform mole comprised of 7.03% and among Hydatidiform mole, partial mole was more common. Dilatation and curettage (82.8%) was the common procedure in compare to hysterectomy for the evaluation of endometrial lesions. Conclusions: In this study, we observed a variety of endometrial lesions. Most of them are benign; among benign, proliferative endometrium was the common histopathological diagnosis followed by disorder proliferative endometrium. Most common presenting age group was found to be at 36 - 45 years. In evaluation of hydatidiform mole, partial mole was more frequent in compare to complete mole. Conventional dilatation and curettage is the preferred method in developing countries with limited resource to screen endometrial lesion and therefore biopsy should be sent for histopathological examination. Thus histopathological examination of routinely stained hematoxylin and eosin is readily available and widely accepted standard technique for evaluation of the endometrial lesions.

scholarly journals Comparison of Different Blood Collection, Sample Matrix, and Immunoassay Methods in a Prenatal Screening Setting

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jeroen L. A. Pennings ◽  
Jacqueline E. Siljee ◽  
Sandra Imholz ◽  
Sylwia Kuc ◽  
Annemieke de Vries ◽  
...  
Keyword(s):  
Prenatal Screening ◽  
Protein A ◽  
Correlation Coefficients ◽  
First Trimester ◽  
Dried Blood Spots ◽  
Maternal Blood ◽  
Blood Collection ◽  
Antibody Microarray ◽  
Sample Matrix ◽  
Finger Prick

We compared how measurements of pregnancy-associated plasma protein A (PAPP-A) and the free beta subunit of human chorionic gonadotropin (fβ-hCG) in maternal blood are influenced by different methods for blood collection, sample matrix, and immunoassay platform. Serum and dried blood spots (DBS) were obtained by venipuncture and by finger prick of 19 pregnant women. PAPP-A and fβ-hCG from serum and from DBS were measured by conventional indirect immunoassay on an AutoDELFIA platform and by antibody microarray. We compared methods based on the recoveries for both markers as well as marker levels correlations across samples. All method comparisons showed high correlations for both marker concentrations. Recovery levels of PAPP-A from DBS were 30% lower, while those of fβ-hCG from DBS were 50% higher compared to conventional venipuncture serum. The recoveries were not affected by blood collection or immunoassay method. The high correlation coefficients for both markers indicate that DBS from finger prick can be used reliably in a prenatal screening setting, as a less costly and minimally invasive alternative for venipuncture serum, with great logistical advantages. Additionally, the use of antibody arrays will allow for extending the number of first trimester screening markers on maternal and fetal health.

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