BOAR, BREED AND ENVIRONMENTAL FACTORS INFLUENCING SEMEN CHARACTERISTICS OF BOARS USED IN ARTIFICIAL INSEMINATION

1984 ◽  
Vol 64 (4) ◽  
pp. 833-843 ◽  
Author(s):  
B. W. KENNEDY ◽  
J. N. WILKINS
Keyword(s):  
Volume Concentration ◽  
Seasonal Pattern ◽  
Sperm Concentration ◽  
Seasonal Effects ◽  
Maximum Volume ◽  
Semen Volume ◽  
Day Of The Week ◽  
Boar Semen ◽  
Live Sperm ◽  
Young Boars

A total of 12 717 ejaculates from 74 Yorkshire, 25 Hampshire, 35 Duroc, 27 Landrace and 5 Lacombe boars were measured for semen volume, sperm concentration, percentage live sperm, motility and potential doses of semen. The data were collected by the Ontario Swine AI Association between 1971 and 1980. Boar repeatability and the effects of breed of boar, technician, year, month, day of the week, interval between collections and age of boar were examined. Boar repeatabilities were 0.21 for volume, 0.32 for concentration, 0.28 for percentage live sperm, 0.21 for motility score and 0.30 for potential doses. Breed of boar effects were significant for all semen measures. Potential doses were highest for Yorkshire (14.7), followed by Landrace (13.7), Duroc (12.9), Hampshire (12.5) and Lacombe (7.7). Differences between technicians were significant for all measures except potential doses. Technicians who collected greater semen volume obtained ejaculates with lower sperm concentration, which resulted in no real difference between technicians for potential doses. Similarly, day of collection was significant for all measures except potential doses. Volume of semen collected was lowest on Saturdays, but Saturday collections had the highest sperm concentration, percentage live sperm and motility scores. Seasonal effects were significant on all traits. Semen volume was lowest in April and increased steadily to peak in November whereafter volume declined. The seasonal pattern for concentration was less definite. Percentage live sperm and motility were highest in January and declined steadily to a low point in August. Potential doses were highest from November to January (13.2–14.2) and lowest from April to June (10.7–10.9). Year effects were also significant and potential doses increased over time. As interval between collections increased, there was as significant increase in semen volume, concentration and potential doses, but interval had little effect on percentage live sperm and motility. Age of boar effects were significant for all traits. Maximum volume, concentration and potential doses were from 24- to 29-mo old boars and lowest volume, concentration and potential doses were from young boars of less than 9 mo. However, percentage live sperm and motility were highest for young boars and declined with increasing age. Key words: Boar, semen, repeatability, breed, age, collection interval

scholarly journals Cryopreservation and quality assessment of boar semen collected from bulk samples

2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Bulk Sample ◽  
Energy Status ◽  
Semen Volume ◽  
Boar Semen ◽  
Live Sperm ◽  
Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P < 0.05) had a lower mean sperm concentration (P < 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P < 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

Effect of different extenders on quality of frozen-thawed boar semen

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
S Frydrychová ◽  
A Lustyková ◽  
E Václavková ◽  
J Lipenský ◽  
M Rozkot
Keyword(s):  
Sperm Motility ◽  
Aspartate Aminotransferase ◽  
Sperm Concentration ◽  
Holding Time ◽  
Holding Period ◽  
Semen Volume ◽  
Significant Difference ◽  
Boar Semen ◽  
Fresh Semen

The objective of this study was to investigate the effect of using different extenders <italic>viz.</italic> Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed. Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.

EVALUATION OF SEMEN FROM YOUNG BEEF BULLS

1968 ◽  
Vol 48 (3) ◽  
pp. 347-352
Author(s):  
H. B. Jeffery ◽  
R. T. Berg ◽  
R. M. Gratz
Keyword(s):  
Volume Concentration ◽  
Sperm Concentration ◽  
Progeny Test ◽  
Motile Sperm ◽  
Progressive Motility ◽  
Semen Volume ◽  
Beef Bulls ◽  
One Year

Semen was collected over a 30-day period from 18 yearling beef bulls, nine Herefords and nine hybrids. Average age at the start of the test was 380 ± 20 days. A total of 112 attempted collections resulted in 94 samples. Fifty percent of ejaculates were considered acceptable for freezing from Herefords and 79% from hybrids, respectively. The hybrid bulls produced significantly more semen per ejaculate (3.3 vs. 2.2 ml), higher sperm concentration (1092 × 106 vs. 657 × 106 per ml), higher sperm numbers per ejaculate (3,834 × 106 vs. 1,499 × 106) and higher total motile sperm per ejaculate (1,999 × 106 vs. 669 × 106). Bull-within-breed variance was significant for semen volume, concentration, initial motility and progressive motility. Progressive motility was in favor of semen from the hybrid bulls but was not statistically significant (48% vs. 36%). It is suggested that the superiority of the hybrid bulls was related to earlier onset of puberty. The trial also suggests that useable semen for progeny test purposes can be obtained soon after bulls reach one year of age.

scholarly journals Incidence and impact of hyperviscosity on sperm parameters of Malawian men seeking assisted reproduction

2020 ◽  
Vol 20 (1) ◽  
pp. 1-3
Author(s):  
Fanuel Lampiao ◽  
Joseph Chisaka
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Volume Concentration ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Infertility Treatment ◽  
Normal Morphology ◽  
Progressive Motility ◽  
Accessory Sex Glands ◽  
Semen Volume

Background: Seminal hyperviscosity has been shown to be associated with male infertility. The aim of this study was to assess the prevalence of hyperviscosity in semen of Malawian males seeking infertility treatment. Methods: A total of 120 men visiting our laboratory for fertility assessment donated semen samples. The semen samples were assessed for hyperviscosity, volume, concentration, total motility, progressive motility, viability, and morphology. Results: Out of the 120 samples analyzed, 34 samples were hyperviscous representing 28.3%. No significant statistical differ- ence in semen volume between samples with normal viscosity compared to those with hyperviscosity (p>0.05). Sperm concen- tration, progressive motility, total motility, viability, and normal morphology were significantly higher in the normal viscosity group when compared to the abnormal viscocity group (p<0.05). Conclusion: Hyperviscosity affects a significant number of men in Malawi and may be the cause of decreased fertility as it was associated with poor sperm concentration, total motility, progressive motility, viability, and morphology. Keywords: Viscosity; sperm motility; accessory sex glands; infertility; semen; semen analysis; spermatozoa.

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

116. EFFECTS OF SUPPLEMENTAL VITAMIN ON THE SEMEN CHARACTERISTICS OF MARKHOZ GOATS DURING NON-BREEDING SEASON

2009 ◽  
Vol 21 (9) ◽  
pp. 35
Author(s):  
A. Towhidi ◽  
A. Farshad ◽  
M. Dolatpanah ◽  
R. Salehi
Keyword(s):  
Vitamin E ◽  
Breeding Season ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Supplemental Vitamin ◽  
Diet Control ◽  
Live Sperm

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.

scholarly journals PRELIMINARY OBSERVATIONS ON THE SEMINAL CHARACTERISTICS OF RABBITS RAISED IN TWO LOCATIONS IN SOUTHEASTERN NIGERIA.

2021 ◽  
Vol 22 (2) ◽  
pp. 108-112
Author(s):  
U. Herbert ◽  
C. O. Acha
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Southeastern Nigeria ◽  
Abnormal Sperm ◽  
Port Harcourt ◽  
Semen Volume ◽  
Live Sperm ◽  
Artificial Vagina ◽  
Time Of Collection

A total of sixteen rabbits sourced from two locations in Southeastern Nigeria were used for this study. Semen was collected from all the rabbits twice weekly for eight weeks using an artificial vagina while observation on the effect of time of collection on semen quality were also made. The results obtained indicated that time of collection had no significant effect (P > 0.05) on all the seminal characteristics measured. However, source of the animals showed significant effect (P < 0.05) on semen volume, sperm concentration, total sperm per ejaculate and proportion of abnormal sperm. Average semen volume, sperm concentration and total sperm per ejaculate were: 0.69±0.007 ml, 104.0±1.44 x 106/m1 and 71.40±0.90x106 respectively for the rabbits sourced from Port Harcourt. The corresponding values for rabbits sourced from Owerri were: 0.66±0.008m1, 96.02 ±1.63x106iml and 63.49±1.50 x 106 respectively. Abnormal sperm proportions were 11.05±0.22% and 1216 ± 032% for the rabbits sourced from Port Harcourt and Owerri respectively. There were no significant (P> 0.05) differences observed between locations in live sperm concentration and sperm motility. Live sperm concentration and sperm motility were 87.75±1.09% and 71.62±0.74% for the Port Harcourt group, while the values obtained for the Owerri group were 88.06±034% and 71.19±0.89% respectively.  

scholarly journals Polymorphism of the GPX-5 gene and characteristics of boar semen

2004 ◽  
Vol 47 (2) ◽  
pp. 165-171 ◽  
Author(s):  
M. Mackowski ◽  
M. Switonski ◽  
J. Mackowska ◽  
W. Perz
Keyword(s):  
Glutathione Peroxidase ◽  
Sperm Concentration ◽  
Polymorphic Site ◽  
Sperm Number ◽  
Sperm Production ◽  
Intron 1 ◽  
Semen Volume ◽  
Boar Semen

Abstract. Two polymorphisms at intron 1 of the glutathione peroxidase 5 (GPX-5) gene, namely A>G substitution (1B and 2B alleles) and deletion/insertion (1C and 2C alleles), were studied in a group of 140 AI boars. Alleles 2B and 1C were the most frequent ones – 0.72 and 0.93, respectively. Only two genotypes for each polymorphic site could be analysed with regard to three characteristics of semen: volume, sperm concentration and total sperm number in the ejaculate. Altogether, data on 13172 ejaculates were used in this study. It was found that 1B2B genotype was related to higher sperm concentration, while other traits demonstrated variable relationships with the genotypes, depending on the analysed breed. It may suggest that the GPX-5 gene is linked to an unknown QTL controlling sperm production.

scholarly journals Study on buck evaluation based on semen quality and fertility

2013 ◽  
Vol 42 (2) ◽  
pp. 101-108 ◽  
Author(s):  
F Sultana ◽  
SS Husain ◽  
A Khatun ◽  
AS Apu ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Fertility Data ◽  
Three Stages ◽  
Live Sperm

The experiment was conducted to evaluate the performance of buck on the basis of semen quality and fertility. Data on 162 ejaculates from five adult Black Bengal bucks (B1, B2, B3, B4 and B5) were recorded. Individual buck effect was found to be significant on semen volume (p<0.05), three stages of sperm motility (p<0.01) and percentage of live sperm (p<0.01) but insignificant (p>0.05) on sperm concentration and percentage of normal sperm. The results of semen volume per ejaculate, sperm concentration, initial sperm motility, on dilution sperm motility, post-thawing sperm motility, percentage of live sperm and percentage of normal sperm were ranged from 0.58±0.17 to 1.04±1.1 ml, 2827±0.76 to 3132

scholarly journals Changes in boar semen with a high and low level of morphologically abnormal spermatozoa

2011 ◽  
Vol 50 (No. 7) ◽  
pp. 289-299 ◽  
Author(s):  
J. Čeřovský ◽  
S. Frydrychová ◽  
A. Lustyková ◽  
M. Rozkot
Keyword(s):  
Performance Test ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Test Results ◽  
Semen Volume ◽  
Significant Difference ◽  
Group A ◽  
Boar Semen ◽  
Group B ◽  
Selection Of

The aim of the paper was to find out the level of changes in the sperm quality in two groups of boars in the insemination (A and B) with diametrically different contents of morphologically abnormal spermatozoa (AS) with an interval of 25 weeks between semen collection I and collection II. In the group A there were 22 boars with the AS content up to 10%, in the group B 16 of boars with the AS content above 40% in collection I. Both groups were comparable concerning the parameters of the performance test results and in quantitative parameters of the semen from collection I. They differed significantly in the AS content (P &lt; 0.01) and in the age (P &lt; 0.05). In collection II in both groups the semen volume increased significantly (A &ndash; P &lt; 0.01; B &ndash; P &lt; 0.05), in the group A the number of spermatozoa per ejaculate and per day also increased (P &lt; 0.01). In the group B there was an insignificant clear decrease in the sperm concentration (P &gt; 0.05). In comparison with the group B the group A can be characterized as a group with significantly higher dynamics in the sperm production per ejaculate. An opposite trend was noted in the total AS content. In the group A there was a significant increase (P &lt; 0.001) and in the group B a significant decrease (P &lt; 0.001) in collection II. In the group A there was a deterioration of the AS content in 7 boars (31.8%), in the group B an improvement in 7 boars (43.7%). Particular monitored AS forms are given. A significant difference in the total AS content between both groups was in favour of the group A (P&lt; 0.001). While no boars from the group A exceeded the limit in the AS content for the applicability of semen for insemination (in theCzech &nbsp; Republic25%), in the group B they remained above this limit without applicability possibility. The detected variations and prevailing stability in the AS occurrence in boars kept in the same conditions lead us to a consideration of hereditary characteristics of the spermatogenesis factor, of considerable persistence of the level of monitored characteristics and to a consideration of applicability of the phenotype AS presentation to selection of boars for artificial insemination. &nbsp; &nbsp;

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