GROWTH OF MICE PRODUCED BY MALES WITH OR WITHOUT THE RAT GROWTH HORMONE TRANSGENE

1990 ◽  
Vol 70 (3) ◽  
pp. 979-982 ◽  
Author(s):  
J. NAGAI ◽  
G. DAVIS ◽  
C. Y. LIN
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Key Words ◽  
Genetic Background ◽  
Growth Hormone Gene ◽  
Male Mice ◽  
Adult Weight ◽  
Rat Growth

Male mice with or without the rat growth hormone transgene were mated with females of three lines without the transgene to examine body weight of resulting crossbred progeny at 21 and 42 d of age. The F1 progeny of sires with the transgene were consistently 16% heavier (P < 0.01) in 42-d body weight than F1 progeny of sires without the transgene in the three dam lines. The former progeny were also significantly (P < 0.01) heavier than the latter at weaning at 21 d of age. Progeny from backcrossing to sires with the transgene were, on average, 17% heavier (P < 0.01) at 42-d than those from sires without the transgene. Thus, sires with the rat growth hormone gene increased body weight of progeny regardless of the genetic background of dams to which sires were mated, and effects of the sires with the gene were clear as early as at weaning of progeny. Key words: Transgene, mice, growth, adult weight

Posttranscriptional regulation of rat growth hormone gene expression: increased message stability and nuclear polyadenylation accompany thyroid hormone depletion

1992 ◽  
Vol 12 (6) ◽  
pp. 2624-2632
Author(s):  
D Murphy ◽  
K Pardy ◽  
V Seah ◽  
D Carter
Keyword(s):  
Growth Hormone ◽  
Thyroid Hormone ◽  
Pituitary Gland ◽  
Anterior Pituitary ◽  
Posttranscriptional Regulation ◽  
Anterior Pituitary Gland ◽  
Growth Hormone Gene ◽  
Gh Gene ◽  
Rat Growth ◽  
Pituitary Glands

In thyroid hormone-depleted rats, the rate of transcription of the growth hormone (GH) gene in the anterior pituitary gland is lower than the rate in euthyroid controls, and there is a corresponding reduction in the abundance of the GH mRNA. Concomitantly, the poly(A) tail of the GH mRNA increases in length. Examination of nuclear RNA from anterior pituitary glands of control and thyroid hormone-depleted rats revealed no difference in the length of pre-mRNAs containing the first and last introns of the GH gene. However, mature nuclear GH RNA is differentially polyadenylated in euthyroid and hypothyroid animals. We suggest that the extent of polyadenylation of the GH transcript is regulated in the cell nucleus concomitant with or subsequent to the splicing of the pre-mRNA. Experiments with anterior pituitary gland explant cultures demonstrated that the GH mRNA from thyroid hormone-depleted rats is more stable than its euthyroid counterpart and that the poly(A) tail may contribute to the differential stability of free GH ribonucleoproteins.

scholarly journals 434 Late-Breaking: Expression of Growth hormone Gene (GH) and Insulin-like growth factor (IGF-2) in metabolic tissues of pigs are affected by unconventional dietary feed sources

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 42-43
Author(s):  
Ufuoma G Sorhue ◽  
Ikenna Omeje ◽  
Ifeanyichukwu udeh ◽  
Adimabua M Moemeka ◽  
Raphael E Onainor
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Weight Gain ◽  
Growth Factor ◽  
Feed Intake ◽  
Feed Efficiency ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Growth Hormone Gene ◽  
Feed Conversion

Abstract This study was conducted to examine the growth performance and expression patterns of growth hormone gene and insulin-like growth factor in metabolic tissues of pigs fed different unconventional feed sources. A total of 45 growing pigs were randomly assigned to 5 dietary treatments at 9 animals per treatment and 3 animals per replicate. Treatment one (T1) comprise of whole maize (WM) which served as the control, T2: brewers dried grain (BDG), T3: cassava peel meal (CPM), T4: plantain peel meal (PPM) and T5: corn husk meal (CHM) at 35% inclusion in the diet. Data collected and analyzed includes; initial body weight (IBW), final body weight (FBW), average daily feed intake (ADFI), average daily weight gain (ADG), feed conversion ratio (FCR) and feed efficiency (FE). At the end of the feeding trial, three animals per treatment (one animal per replicate) were slaughtered; the duodenum and liver were collected and stored using RNALater prior to RNA extraction. Real-time qPCR was performed in 40cycles using the PowerUp SYBR Green reagent and analysed using the Delta-Delta Ct method. All data were subjected to analysis of variance in a completely randomized design. The results showed that the treatment diets had effect on growth performance with significant differences (P &lt; 0.05) observed for average final weight, average total weight gain, average daily feed intake, feed conversion ratio and feed efficiency while the initial weight were not significantly different (P &gt; 0.05). IGF-2 was differentially expressed in the liver and duodenum across treatments while significant differences were not observed in the expression pattern of the growth hormone gene in both tissues. This study showed promise for the use of unconventional feeding stuffs in pig feeding and reveals that genes influencing performance characters are differentially expressed in relation to the source of unconventional feeds used in compounding pig diet.

scholarly journals Rat growth hormone gene introns stimulate nucleosome alignment in vitro and in transgenic mice.

1995 ◽  
Vol 92 (17) ◽  
pp. 7724-7728 ◽  
Author(s):  
K. Liu ◽  
E. P. Sandgren ◽  
R. D. Palmiter ◽  
A. Stein
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Growth Hormone Gene ◽  
Rat Growth

scholarly journals Interaction of a tissue-specific factor with an essential rat growth hormone gene promoter element.

1987 ◽  
Vol 7 (3) ◽  
pp. 1193-1197 ◽  
Author(s):  
B L West ◽  
D F Catanzaro ◽  
S H Mellon ◽  
P A Cattini ◽  
J D Baxter ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Dna Sequences ◽  
Site Directed Mutagenesis ◽  
Specific Factor ◽  
Growth Hormone Gene ◽  
Base Pairs ◽  
Hybrid Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Rat Growth

Rat growth hormone (rGH) gene expression is normally restricted to the anterior pituitary. As a model of this tissue specificity, we compared the transient expression of an rGH-chloramphenicol acetyltransferase (CAT) hybrid gene in rGH-producing rat pituitary tumor (GC) cells and in non-rGH-producing rat fibroblast (rat-2) cells. Deletion analysis of the rGH portion of this hybrid gene demonstrated that DNA sequences within 140 base pairs 5' to the rGH gene were sufficient for correct cell type-specific expression. Deletion of an additional 35 base pairs of the rGH 5'-flanking DNA resulted in a loss of expression of the transfected hybrid gene and correlated with the interaction of a putative trans-acting factor with this region of the rGH promoter. This factor was detectable by DNase I footprinting in a crude nuclear extract from GC cells but not from rat-2 cells. Site-directed mutagenesis of the footprint region caused complete loss of expression of a hybrid gene containing 530 base pairs 5' to the rGH gene. Thus, the interaction of this factor, which we term GC2, is likely to be essential for the tissue-specific expression of the rGH gene.

The influence of dietary monensin and salinomycin on growth and endocrine status of gilts

1992 ◽  
Vol 72 (2) ◽  
pp. 427-429 ◽  
Author(s):  
T. A. Van Lunen ◽  
R. N. Kirkwood ◽  
P. A. Thacker
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Key Words ◽  
Crude Protein ◽  
Protein Diet ◽  
Serum Growth Hormone ◽  
Endocrine Status ◽  
Effect Of Treatment

Seventy-two gilts were fed a 16% crude protein diet supplemented with monensin, salinomycin or not supplemented. There was no effect of treatment on gilt growth between 30 and 100 kg body weight. Monensin-fed gilts tended to have increased, while salinomycin-fed gilts had decreased (P < 0.01), serum growth hormone concentrations. Salinomycin-fed gilts were older and heavier at puberty (P < 0.05). Key words: Monensin, salinomycin, gilts, growth, reproduction

scholarly journals Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

1984 ◽  
Vol 12 (18) ◽  
pp. 7153-7173 ◽  
Author(s):  
Arthur Gutierrez-Hartmann ◽  
Ivan Lieberburg ◽  
David Gardner ◽  
John D. Baxter ◽  
Guy G. Cathala
Keyword(s):  
Growth Hormone ◽  
Repetitive Dna ◽  
Tandem Repeat ◽  
Growth Hormone Gene ◽  
Repeat Structure ◽  
Rat Growth

scholarly journals The aromatase inhibitor letrozole increases epiphyseal growth plate height and tibial length in peripubertal male mice

2004 ◽  
Vol 182 (1) ◽  
pp. 165-172 ◽  
Author(s):  
R Eshet ◽  
G Maor ◽  
T Ben Ari ◽  
M Ben Eliezer ◽  
G Gat-Yablonski ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Aromatase Inhibitor ◽  
Growth Plate ◽  
Growth Potential ◽  
Longitudinal Growth ◽  
Male Mice ◽  
Epiphyseal Growth Plate ◽  
Plate Height ◽  
Igf I

Sex hormones may influence longitudinal growth, either indirectly, by affecting the growth-hormone-insulin-like growth factor I (IGF-I) axis, or directly, by affecting changes within the epiphyseal growth plate (EGP). The aim of the present study was to investigate the effects of letrozole, an aromatase inhibitor, on longitudinal growth and changes in the EGP in vivo. Eighteen peripubertal male mice were divided into three groups. The first group was killed at baseline, the second was injected with letrozole (Femara) s.c., 2 mg/kg body weight/day, for 10 days, and the third was injected with the vehicle alone. Serum testosterone levels were found to be significantly higher in the treated group than in the controls. Letrozole induced a significant increase in body weight, tail length and serum growth hormone level, but had no significant effect on the level of serum IGF-I. On histomorphometric study, there was a significant increase (12%) in EGP height in the treated animals compared with controls. Immunohistochemistry showed a 3.4-fold letrozole-induced increase in the proliferation of the EGP chondrocytes, as estimated by the number of proliferation cell nuclear antigen-stained cells, and a decrease in the differentiation of the EGP chondrocytes, as estimated by type X collagen staining. Letrozole did not interfere with type II collagen levels. The study group also showed a twofold increase in the number of IGF-I receptor-positive cells compared with controls. In conclusion, the aromatase inhibitor, letrozole, appears to increase the linear growth potential of the EGP in mice.

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