The prevalence of Fusarium spp. colonizing seed corn stalks in southwestern Ontario, Canada

2009 ◽  
Vol 89 (1) ◽  
pp. 103-106 ◽  
Author(s):  
L. Tamburic-Ilincic ◽  
A. W. Schaafsma

Gibberella zeae, Fusarium verticillioides and F. subglutinans are the most important causes of Fusarium stalk rot in corn (Zea mays L.). Gibberella zeae also causes fusarium head blight in wheat (Triticum aestivum L.) and gibberella ear rot in corn. The objectives of this study were to investigate prevalence of Fusarium species in the stalks of seed corn over time and to investigate the influence of sampling time and internode position on Fusarium spp. and G. zeae, particularly. Fusarium subglutinans and G. zeae were the most frequently recovered species from asymptomatic host tissue and from pink discoloration on stalks, respectively. More G. zeae was isolated from the basal internode of stalks than from the higher ones closer to harvest time. Other species isolated from seed corn stalks over time included F. verticillioides, F. oxysporum, F. sporotrichioides and F. equiseti. A similar spectrum of Fusarium species was identified from corn ears and from winter wheat across southwestern Ontario. Key words: Zea mays L., Fusarium spp.

2018 ◽  
Vol 7 (1) ◽  
pp. 31-49
Author(s):  
Narges Atabaki ◽  
Vahid Rahjoo ◽  
Mohamed M. Hanafi ◽  
Rambod Abiri ◽  
Hamidreza Z. Zadeh ◽  
...  

Fusarium verticillioides and Fusarium proliferatum cause a wide range of maize diseases.  These fungi produce dangerous mycotoxins, such as fumonisin B1, which are important threats to humans and animals. Given this predicament, the present study aimed to identify the fungi both molecular-morphologically and also investigate the pathogenicity variation and mating type of 41 Fusarium strains in maize (Zea mays L.) samples with sifting their fumonisin contents.  Furthermore, species-specific primers for the molecular identification of distinct strains amplified 2 fragments of 578 and 800 bp in Fusarium verticillioides, while a single 585 bp band was amplified in Fusarium proliferatum.  Accordingly, 24 isolates out of 41 were identified as F. verticillioides, and 13 isolates were identified as F. proliferatum.  The fumonisin-producing and non-producing Fusarium strains were identified using the VERTF-1/VERTF-2 primers.  A total of 24 isolates of F. verticillioides were positively scored based on the amplification of a single 400 bp fragment.  The highest and lowest fumonisin content, as measured using an enzyme-linked immunosorbent assay (ELISA), belonged to strains MS1 and MG3, respectively, and ranged from 960-12673 and 4.07-23 ppm, respectively.  Additionally, the mating type test showed that the sexual form of the studied Fusarium species could possibly belong to the A and D mating populations.  In vivo and in vitro pathogenicity tests revealed a high susceptibility.


Toxins ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 224 ◽  
Author(s):  
Karolina Gromadzka ◽  
Lidia Błaszczyk ◽  
Jerzy Chełkowski ◽  
Agnieszka Waśkiewicz

Maize has become one of the most important crops for food and feed production—both as a silage and crop residue worldwide. The present study aimed to identify the co-occurrence of Fusarium subglutinans, Fusarium verticillioides, Trichoderma atroviride, Sarocladium zeae, and Lecanicillium lecanii on maize ear rot. Further, the accumulation of mycotoxins as secondary metabolites of Fusarium spp. in maize ear samples was also analyzed. Maize ear samples were collected between 2014 and 2017 from two main maize growing areas in Poland (Greater Poland and Silesia region). A significant difference was found in the frequency of two main Fusarium spp. that infect maize ears, namely F. subglutinans and F. verticillioides. In addition to Fusarium spp. T. atroviride, S. zeae, and L. lecanii were also identified. T. atroviride species was found in 14% of maize samples examined between 2014 and 2017, particularly with a high percentage of Trichoderma spp. recorded in 2014, i.e., in 31% of samples. However, mycotoxin content (beauvericin and fumonisins) varied, depending on both the location and year of sampling. The interaction of fungi and insects inhabiting maize ear and kernel is very complex and not yet elucidated. Therefore, further research is required in this area.


2018 ◽  
Vol 15 ◽  
pp. 30-37 ◽  
Author(s):  
Olumayowa Mary Olowe ◽  
Odunayo Joseph Olawuyi ◽  
Ayodele Adegboyega Sobowale ◽  
Adegboyega Christopher Odebode

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1161
Author(s):  
Andrés Gustavo Jacquat ◽  
Martín Gustavo Theumer ◽  
María Carmen Cañizares ◽  
Humberto Julio Debat ◽  
Juliana Iglesias ◽  
...  

Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.


2020 ◽  
Vol 26 ◽  
pp. e00474
Author(s):  
A.O. Akanmu ◽  
A.A. Sobowale ◽  
M.A. Abiala ◽  
O.J. Olawuyi ◽  
A.C. Odebode

EUGENIA ◽  
2012 ◽  
Vol 18 (3) ◽  
Author(s):  
Vilma Victa Rikumahu ◽  
Jantje Pongoh ◽  
J. M. Paulus

ABSTRACT Research has been implemented as of July 2012, in the  BPSB Laboratory  of North Sulawesi Province i.e.: 1) to test the seed lacking in vigour during the Random Complete Design with three treatments of the various harvest ages, i.e. U1 (90 dasy), U2 (100 days) and U3 (110 days) treatments repeated four times; 2) in the greenhouse to see the amount of the normal, sprouts abnormal, seeds, hard seed and dead, shoot root ratio by using the Random Completed Design with two factors, respectively are : factorial in complete factors of the first different age harvest U1 (90 days), U2 (100 days) and U3 (110 days), the second factor granting of the water with different capacity P1 (3600 ml), P2 (2700 ml), P3 (1800 ml) and P4 (900 ml). Data analysis were found by the various analysis, however if there are different results, it would continue on the Smallest Significant Test. The results showed that, Electric Power Conductivity (EPC) affected by the age of harvest seed corn. The highest EPC at the harvest ages of 90 days and the lowest at 100 days and 110 days. It would be shown that the great value of EPC, the smallest amount of germination. There is no interaction between the age of abnormal seeds, hard seed die, shoot root ratio and high sprouts, but single factor treatment of harvest age and media planting humidity were significantly effected toward sprout normal, abnormal seed, hard seed die, shoot root ratio and high sprouts. Keywords : corn seed, harvest ages, humidity ABSTRAK   Penelitian dilaksanakan sejak bulan Juli 2012, penelitian pertama di Laboratorium Balai Penelitian dan Sertifikasi Benih (BPSB) Provinsi Sulawesi Utara, untuk menguji vigor benih menggunakan Rancangan Acak Lengkap dengan tiga perlakuan umur panen yang berbeda: U1 (90 Hari), U2 (100 Hari) dan U3 (110 hari) perlakuan diulang empat kali. Penelitian kedua di Rumah Kaca untuk melihat jumlah kecambah normal, abnormal, benih keras,benih mati dan nisbah pupus akar  menggunakan Rancangan Acak Lengkap disusun secara faktorial  dengan  2 faktor  masing-masing adalah : faktor pertama : Umur Panen yang berbeda, U1 (90 Hari), U2 (100 Hari) dan U3 (110 hari). faktor kedua pemberian air dengan kapasitas yang berbeda, P1 (3600 ml), P2 (2700 ml), P3 (1800 ml) dan P4 (900 ml). Data yang diperoleh menggunakan analisa ragam dan jika terdapat perbedaan perlakuan dilanjutkan dengan uji Beda Nyata Terkecil  (BNT). Hasil penelitian yang didapat adalah; Daya hantar listrik (DHL) dipengaruhi oleh umur panen benih jagung, DHL tertinggi pada umur panen 90 hari dan yang terendah umur panen 100 dan 110 hari. Hal ini menunjukan bahwa semakin besar nilai DHL semakin kecil jumlah perkecambahan. Tidak terdapat interaksi antara umur panen benih dan kelembaban media tanam terhadap jumlah kecambah normal, abnormal, benih keras, benih mati, nisbah pupus akar dan tinggi kecambah tetapi secara tunggal faktor perlakuan umur panen dan kelembaban media tanam berpengaruh nyata terhadap kecambah normal, abnormal, benih keras, benih mati, nisbah pupus akar dan tinggi kecambah. Kata kunci : benih jagung, umur panen, kelembaban


Plant Disease ◽  
2004 ◽  
Vol 88 (7) ◽  
pp. 724-730 ◽  
Author(s):  
S. A. Pereyra ◽  
R. Dill-Macky ◽  
A. L. Sims

Survival and inoculum production of Gibberella zeae (Schwein.) Petch (anamorph Fusarium graminearum (Schwabe)), the causal agent of Fusarium head blight of wheat and barley, was related to the rate of wheat (Triticum aestivum L.) residue decomposition. Infested wheat residue, comprising intact nodes, internodes, and leaf sheaths, was placed in fiberglass mesh bags on the soil surface and at 7.5- to 10-cm and 15- to 20-cm depths in chisel-plowed plots and 15 to 20 cm deep in moldboard-plowed plots in October 1997. Residue was sampled monthly from April through November during 1998 and every 2 months through April to October 1999. Buried residue decomposed faster than residue placed on the soil surface. Less than 2% of the dry-matter residue remained in buried treatments after 24 months in the field, while 25% of the residue remained in the soil-surface treatment. Survival of G. zeae on node tissues was inversely related to the residue decomposition rate. Surface residue provided a substrate for G. zeae for a longer period of time than buried residue. Twenty-four months after the initiation of the trial, the level of colonization of nodes in buried residue was half the level of colonization of residue on the soil surface. Colonization of node tissues by G. zeae decreased over time, but increased for other Fusarium spp. Ascospores of G. zeae were still produced on residue pieces after 23 months, and these spores were capable of inducing disease. Data from this research may assist in developing effective management strategies for residues infested with G. zeae.


1962 ◽  
Vol 42 (2) ◽  
pp. 302-307 ◽  
Author(s):  
N. J. Whitney ◽  
C. G. Mortimore

In bio-assay tests ether extracts of the roots and of the stalks of both resistant and susceptible corn inbreds inhibited the growth of Fusarium moniliforme Sheld. and Gibberella zeae (Schw.) Petch, the level of inhibition varying over the season. However, the extracts of the resistant and susceptible inbreds did not differ consistently in their ability to inhibit fungus growth on any given date. Thus, this bio-assay method cannot be used to evaluate inbreds for resistance to root and stalk rot.


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