Identification of Brassica genotypes and molecular markers for increased seed oil content

2014 ◽  
Vol 94 (6) ◽  
pp. 1103-1108 ◽  
Author(s):  
Nasir Javed ◽  
Muhammad Tahir ◽  
Jianfeng Geng ◽  
Genyi Li ◽  
Peter B. E. McVetty

Javed, N., Tahir, M., Geng, J., Li, G. and McVetty, P. B. E. 2014. Identification of Brassica genotypes and molecular markers for increased seed oil content. Can. J. Plant Sci. 94: 1103–1108. Carbon dioxide emissions by the transportation sector are major contributors to global climate change. Lower CO2 emissions by the transportation sector are linked to the use of renewable fuels including biodiesel. Canola has high seed oil content, adaptation to temperate climates and favorable fatty acid composition, which make it a preferred feedstock for biodiesel production. Doubled haploid (DH) line, random inbred (RI) line and consensus genetics maps for mapping populations derived from Polo × Topas were developed. The DH line-based genetic map was then used for the identification and tagging of quantitative trait loci (QTL) controlling seed oil biosynthesis. This genetic map consisted of 620 loci identified using several different types of molecular markers, and covered a map distance of 2241.1 cM with marker saturation of 3.7 cM. The phenotypic data on the mapping population for seed oil content and component fatty acids were collected from four-environment replicated field trials. One hundred and thirty-one QTL for various fatty acids in canola oil and 14 QTL for oil content were identified. These QTL, combined with marker-assisted selection, may assist breeders in their attempts to develop canola lines with improved oil quality, oil content and oil production per hectare for biodiesel production.

2021 ◽  
Vol 304 ◽  
pp. 01002
Author(s):  
Abdelhamid Benmoumou ◽  
Saïd El Madidi

Citrullus colocynthis has been garnering interest in recent times as a potential biodiesel feed stockcrop due to its high seed oil content and its natural adaptation to drought The variability seed oil content (OC), Oil yield (kg/ha) (OY) and fatty acid composition were investigated for 12 accessions collected in different localities in Morocco. Analysis of the data revealed high variability among the accessions, with seed oil content ranging between 17.1 and 24.3 % of seed weight and Oil yield from 35.3 to 172.7 kg/ha. The predominant fatty acid of the seed oil was linoleic acid, C18:2, ranging from 67.0 to 73.0 % of total fatty acids and the average values of unsaturated fatty acids vary between 75.25 and 81.94 %.The wide range of variations found in this study for the traits measured offers the possibility of genetic improvement to develop cultivars for biodiesel production compatible with sustainable and ecological agriculture.


2021 ◽  
Vol 72 (3) ◽  
pp. e415 ◽  
Author(s):  
M. De Wit ◽  
V.K. Motsamai ◽  
A. Hugo

Cold-pressed seed oil from twelve commercially produced cactus pear cultivars was assessed for oil yield, fatty acid composition, physicochemical properties, quality and stability. Large differences in oil content, fatty acid composition and physicochemical properties (IV, PV, RI, tocopherols, ORAC, % FFA, OSI and induction time) were observed. Oil content ranged between 2.51% and 5.96% (Meyers and American Giant). The important fatty acids detected were C16:0, C18:0, C18:1c9 and C18:2c9,12, with C18:2c9,12, the dominating fatty acid, ranging from 58.56-65.73%, followed by C18:1c9, ranging between 13.18-16.07%, C16:0, which ranged between 10.97 - 15.07% and C18:0, which ranged between 2.62-3.18%. Other fatty acids such as C14:0, C16:1c9, C17:0, C17:1c10, C20:0, C18:3c9,12,15 and C20:3c8,11,14 were detected in small amounts. The quality parameters of the oils were strongly influenced by oil content, fatty acid composition and physicochemical properties. Oil content, PV, % FFA, RI, IV, tocopherols, ORAC and ρ-anisidine value were negatively correlated with OSI. C18:0; C18:1c9; C18:2c9,12; MUFA; PUFA; n-6 and PUFA/SFA were also negatively correlated with OSI. Among all the cultivars, American Giant was identified as the paramount cultivar with good quality traits (oil content and oxidative stability).


2020 ◽  
Vol 149 ◽  
pp. 112367
Author(s):  
Mohadeseh Kiani ◽  
Iraj Alahdadi ◽  
Elias Soltani ◽  
Birte Boelt ◽  
Fatemeh Benakashani

2013 ◽  
Vol 162 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Maoyin Li ◽  
Sung Chul Bahn ◽  
Chuchuan Fan ◽  
Jia Li ◽  
Tien Phan ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2048
Author(s):  
Kamel Zemour ◽  
Ahmed Adda ◽  
Amina Labdelli ◽  
Abdelkader Dellal ◽  
Muriel Cerny ◽  
...  

Safflower seeds provide an oil rich in mono and polyunsaturated fatty acids. Its adaptation to drought and high temperatures makes it an alternative for the development of oleaginous crops in semi-arid areas. This study examines the oil content and the chemical composition of seed oil from three safflower accessions (Gila, Halab, Touggourt) cultivated over three years (2015, 2016, and 2017) in a semi-arid area in Tiaret (West of Algeria). Under these semi-arid conditions, characterized by low rainfall and high temperatures, seed oil content remained relatively high and was composed mainly of unsaturated fatty acids, with their ratio to saturated fatty acids reaching an average value of 9. Seed oil content varies between 22.8% and 28.4% among the genotypes and throughout the three years. The extracted oil consists essentially of unsaturated fatty acids, linoleic and oleic acids, poly and monounsaturated, respectively. Their contents over the three years vary between 75% and 79.3% for linoleic acid and between 10.2% and 14.7% for oleic acid. The saturated fatty acids content of the oil reached a maximum value of 9%. They consist mainly of palmitic acid, of which the average genotypic content varies between 6.6% and 7.15% depending on all grown years. The results obtained may assist in better understanding the response of cultivars under rainfed conditions and could be helpful for breeders with regards to introduction into selection programs.


2015 ◽  
Vol 140 (4) ◽  
pp. 362-372 ◽  
Author(s):  
Joyce W. Ngure ◽  
Chunyan Cheng ◽  
Shuqiong Yang ◽  
Qunfeng Lou ◽  
Ji Li ◽  
...  

Cucumber (Cucumis sativus) seed oil has the potential for use as an edible oil and as a pharmaceutical, cosmetic, insecticidal, and industrial product. In this study, we investigated, for the first time, the effect of cultivar and season on seed number, oil content, and fatty acid profiles as well as their proportions in different cucumber cultivars. We examined the effects of spring and autumn seasons on seed oil content and fatty acid composition in 46 cucumber cultivars and one wild species of cucumber (C. anguria) grown in greenhouse experiments in 2013 and 2014. Seed oil was determined using the Soxhlet method and fatty acids using the gas chromatography-mass spectrometry method. Seed oil content in the cucumber seeds ranged from 41.07% in ‘Hazerd’ to 29.24% in ‘Lubao’ while C. anguria had 23.3%. Fatty acids detected were linoleic (C18:2), palmitic (C16:0), oleic (C18:1), stearic (C18:0), linolenic (C18:3), behenic (C22:0), arachidic C20:0), lignoceric (C24:0), eicosenoic (C20:1), palmitoleic (C16:1), and myristic (C14:0), among other unidentified fatty acids. The results showed significant effects of cultivar genotype, growing season, and interactions on the variables examined. The content of seed oil and fatty acids differed significantly among the cultivar genotypes. Spring-grown cucumbers had higher quantities of oil than the autumn-grown cucumbers. The content of fatty acids (mainly palmitic, palmitoleic, stearic, oleic, eicosenoic, and lignoceric) also was higher in spring. In autumn there were more seeds, and higher linoleic, linolenic, and other unspecified fatty acids. The higher the oleic acid content the lower was the linoleic acid indicating a strong negative relationship in these two fatty acids. The higher the seed oil content the higher was linoleic and oleic indicating a positive relationship between the seed oil and the two fatty acids. Results of this study provide important information applicable in improving management and production of cucumber seed oil especially considering its versatility in uses. Furthermore, the wide range of fatty acids found in the studied cucumber cultivars could be used in the production of novel industrial oils through genetic engineering.


2020 ◽  
Vol 14 (1) ◽  
pp. 299-320
Author(s):  
Rostislav Y. Blume ◽  
Genadiy V. Lantukh ◽  
Iryna V. Levchuk ◽  
Kostyantyn M. Lukashevych ◽  
Dzhamal B. Rakhmetov ◽  
...  

Background: One of the most promising alternative biofuels, competitive with regular petrol, diesel or jet fuel is biodiesel, especially derived from plant oils. Until now, various technological approaches, as well as oil sources, have been proposed for biodiesel production, but an industrially scalable technology with high end-product quality and production efficiency has not been developed and brought to the market yet. Biodiesel is produced in Europe and North America mainly from rapeseed, or canola, sunflower and soybean oil. However, other underutilized plant species could also be considered as potential oil feedstocks for biodiesel. The great perspective holds Brassicaceae family, especially such species as false flax (Camelina sativa) and Ethiopian mustard (Brassica carinata), but many other Brassicaceae crops are still out of sight. Objectives: This research has been conducted aiming to identify and compare the productivity of several Brassicaceae crops (camelina or false flax (C. sativa), turnip rape (B. campestris), oil radish (Raphanus sativus var. oleifera) and tyfon (B. rapa ssp. oleifera f. biennis × (ssp. rapifera × ssp. pekinensis)), that are suitable for biodiesel production under conditions of temperate climate regions (Northern America, Europe); and to obtain biodiesel by transesterification of fatty acids present on these species using bioethanol. Methods and Materials: Seed oil content, yield and fatty acid profiles have been studied and analysed in different genotypes of C. sativa (10), winter (6) and spring (4) B. campestris, R. sativus var. oleifera (8) and tyfon (5). The most productive crops have been identified: false flax variety ‘Evro-12’ (1620 kg of oil per hectare) and ‘Peremoha’ (1657 kg/ha); winter turnip rape variety ‘Oriana’ (1373 kg/ha), oil radish variety ‘Kyianochka’ (1445 kg/ha) and tyfon varieties ‘Fitopal’ (1730 kg/ha) and ‘Obriy’ (1860 kg/ha). According to chromatographic analysis results, oils of winter turnip rape and tyfon contain high levels (38-42,8%) of erucic (22:1) acid, while oils from spring turnip rape, false flax and oil radish possess high amounts of short-chained fatty acids (not longer than C18) – up to 85,37% in camelina breeding line FEORZhYaFD. Fatty acid ethyl esters (FAEE) were produced from oil of best genotypes and proved to comply with all main quality requirements for diesel. Results: Moreover, a new solvent-based technology of high-yield (up to 96%) FAEE production, has been firstly proposed for C. sativa oil conversion. Conclusion: Best genotypes that can be used as a plant oil source for biodiesel production have been identified for camelina, turnip rape, oil radish and tyfon species. The data obtained on seed oil content, yield and fatty acid profiles suggested that they are: false flax – breeding form FEORZhYaFD; winter turnip rape - variety ‘Oriana’; oil radish - variety ‘Rayduha’ and tyfon hybrid - variety ‘Fitopal’. Biodiesel samples obtained from these plants fit the Ukrainian standards for diesel fuel and can be used in car engines. The proposed new technological approach to produce fatty acid ethyl esters allows to reduce reaction time and to increase esters yield and quality.


2007 ◽  
Vol 55 (4) ◽  
pp. 485-489 ◽  
Author(s):  
A. Fieldsend

Seed moisture content is a well-recognised index of safe storage. However, when in equilibrium with the storage environment it is merely an indicator of the relative humidity of the air, which is the primary regulator of the growth of moulds and insects. The relationship is influenced by the profile of the seed components. During the 1990s, significant increases in the seed oil content of evening primrose were achieved through plant breeding. This paper shows that the equilibrium moisture content of evening primrose seeds declines significantly with increasing oil content. Hence, the moisture isotherm is altered and newer cultivars must be stored at slightly lower seed moisture contents to ensure that seed and oil quality are maintained.


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