Detection of main-effect and epistatic QTL for yield-related traits in rice under drought stress and normal conditions

2014 ◽  
Vol 94 (4) ◽  
pp. 633-641 ◽  
Author(s):  
Wang Xing ◽  
Hongwei Zhao ◽  
Detang Zou
Keyword(s):  
Drought Stress ◽  
Crop Yields ◽  
Upland Rice ◽  
Recombinant Inbred Lines ◽  
Stress Conditions ◽  
Epistatic Qtls ◽  
Epistatic Qtl ◽  
Main Effect ◽  
Drought Resistant ◽  
Main Effect Qtls

Xing, W., Zhao, H. and Zou, D. 2014. Detection of main-effect and epistatic QTL for yield-related traits in rice under drought stress and normal conditions. Can. J. Plant Sci. 94: 633–641. Drought-resistant cultivars play an important role in maintaining high and stable crop yields under drought-stress conditions. However, the genetic mechanism of drought resistance must first be elucidated. Therefore, 220 recombinant inbred lines from a cross between Xiaobaijingzi (upland rice) and Kongyu 131 (Oryza sativa L.) were used to identify quantitative trait loci (QTLs) for yield and yield-component traits under drought stress and control conditions in Heilongjiang and Tieli. As a result, 23 main-effect QTLs and 11 digenic interactions were detected for four traits under the above two conditions. Of the main-effect QTLs, 10 and 8 were detected under control and drought-stress conditions, respectively; and five common QTLs were observed. In addition, five QTLs were found to be responsible for the difference across the two conditions. Among all epistatic QTLs, three types of epistatic QTLs were observed: one was between two main-effect QTLs, such as qPH-3-1 and qPH-7-2; one was between one locus with and another without main-effect, e.g., qPN-4 and qPN-3-2; and one was between two loci without main-effect, e.g., qYP-6-1 and qYP-12-2. In the above epistatic examples, their recombinant genotypes tended to reduce plant height and the number of grains per panicle and increase yield, respectively. Our results provide a good foundation for designed molecular breeding of drought-resistant rice.

scholarly journals Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

2018 ◽  
Vol 19 (12) ◽  
pp. 4020 ◽  
Author(s):  
Xinbo Wang ◽  
Yanhua Xu ◽  
Jingjing Li ◽  
Yongzhe Ren ◽  
Zhiqiang Wang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Gene Silencing ◽  
Proteomic Analysis ◽  
Crop Yields ◽  
Stress Conditions ◽  
Virus Induced Gene Silencing ◽  
Knock Down ◽  
Comparative Proteomic Analysis ◽  
Related Proteins

Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.

scholarly journals Microbial Diversity of Upland Rice Roots and Their Influence on Rice Growth and Drought Tolerance

2020 ◽  
Vol 8 (9) ◽  
pp. 1329
Author(s):  
Zhiqiang Pang ◽  
Ying Zhao ◽  
Peng Xu ◽  
Diqiu Yu
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Plant Growth ◽  
Upland Rice ◽  
Oryza Sativa L ◽  
Soluble Sugar ◽  
Stress Conditions ◽  
Rice Roots ◽  
Microbial Resources

Among abiotic stresses, drought is one of the most important factors limiting plant growth. To increase their drought tolerance and survival, most plants interact directly with a variety of microbes. Upland rice (Oryza sativa L.) is a rice ecotype that differs from irrigated ecotype rice; it is adapted to both drought-stress and aerobic conditions. However, its root microbial resources have not been explored. We isolated bacteria and fungi from roots of upland rice in Xishuangbanna, China. Four hundred sixty-two endophytic and rhizospheric isolates (337 bacteria and 125 fungi) were distributed. They were distributed among 43 genera on the basis of 16S rRNA and internal transcribed spacer (ITS) gene sequence analysis. Notably, these root microbes differed from irrigated rice root microbes in irrigated environments; for example, members of the Firmicutes phylum were enriched (by 28.54%) in the roots of the upland plants. The plant growth-promoting (PGP) potential of 217 isolates was investigated in vitro. The PGP ability of 17 endophytic and 10 rhizospheric isolates from upland rice roots was evaluated under well-irrigated and drought-stress conditions, and 9 fungal strains increased rice seedling shoot length, shoot and root fresh weight (FW), antioxidant capability, and proline (Pro) and soluble sugar contents. Our work suggests that fungi from upland rice roots can increase plant growth under irrigated and drought-stress conditions and can serve as effective microbial resources for sustainable agricultural production in arid regions.

scholarly journals Water-efficient rice performances under drought stress conditions

2021 ◽  
Vol 6 (3) ◽  
pp. 838-863
Author(s):  
Didi Darmadi ◽  
◽  
Ahmad Junaedi ◽  
Didy Sopandie ◽  
Supijatno ◽  
...  
Keyword(s):  
Drought Stress ◽  
Water Use Efficiency ◽  
Water Use ◽  
Upland Rice ◽  
Stress Conditions ◽  
Root Anatomy ◽  
Shoot Biomass ◽  
Sensitivity Index ◽  
Rice Varieties ◽  
Use Efficiency

<abstract> <p>The use of varieties that are able to adapt well to extreme environments is one strategy to overcome the challenges of decreasing production in sub-optimal land. Indonesian tropical rice varieties (Jatiluhur, IPB 9G, IPB 3S, Hipa 19, Mentik Wangi, Ciherang, Inpari 17, and Mekongga) have been tested and established as water-used-efficient varieties in an optimal environment. However, to date, these varieties have not been examined in the suboptimal area, in particular, drought stress conditions. Therefore, this study aimed to evaluate the adaptation response of production, morphological, and physiological character of several water-efficient rice varieties under drought stress in the field. The study was designed in a split-plot with two factors and 4 replications, where the first factor (main plot) was drought stress stages i.e. vegetative (Dv), reproductive (Dr), generative (Dg), and control (Dc). The second factor was rice varieties, consisting of eight varieties, i.e., Jatiluhur, IPB 3S, IPB 9G, Hipa 19, Mentik Wangi, Ciherang, Inpari 17, and Mekongga. The experiment was conducted from May to December 2018 in Muneng Kidul Village, Probolinggo Regency, East Java Province. The experimental variables were morphology, production, leaf scrolling score during drought stress, drought sensitivity index, water use efficiency, physiology and root anatomy. The result showed that upland rice varieties were more tolerant to drought stress and had a higher water use efficiency than lowland rice varieties. This shows that Jatiluhur and IPB 9G which are indicated to be adaptive to drought stress, and have the ability to regulate water use more efficiently when drought stress occurs. Therefore, water use efficiency could be used as selection characters under drought conditions in rice particularly tropical upland rice. Moreover, morphological characters, i.e., grain yiled per plot, weight of pithy grain, weight of shoot biomass and weight of roots could be the selection characters to predict drought tolerant tropical rice. According to physiological characters, photosynthesis rate, transpiration rate, proline content, malondialdehyde content, leaf water potential and leaf greenness could be used as a selection tool to predict water use efficient genotypes in rice. However, further studies are needed to understand the complex mechanisms of water use efficiency by combining various approaches.</p> </abstract>

Overdominant Epistatic Loci Are the Primary Genetic Basis of Inbreeding Depression and Heterosis in Rice. I. Biomass and Grain Yield

Genetics ◽  
2001 ◽  
Vol 158 (4) ◽  
pp. 1737-1753 ◽  
Author(s):  
Zhi-Kang Li ◽  
L J Luo ◽  
H W Mei ◽  
D L Wang ◽  
Q Y Shu ◽  
...  
Keyword(s):  
Grain Yield ◽  
Inbreeding Depression ◽  
Genetic Basis ◽  
Mixed Model ◽  
Recombinant Inbred Lines ◽  
Hybrid Breakdown ◽  
Mixed Model Approach ◽  
Epistatic Qtl ◽  
Main Effect ◽  
Mapping Populations

AbstractTo understand the genetic basis of inbreeding depression and heterosis in rice, main-effect and epistatic QTL associated with inbreeding depression and heterosis for grain yield and biomass in five related rice mapping populations were investigated using a complete RFLP linkage map of 182 markers, replicated phenotyping experiments, and the mixed model approach. The mapping populations included 254 F10 recombinant inbred lines derived from a cross between Lemont (japonica) and Teqing (indica) and two BC and two testcross hybrid populations derived from crosses between the RILs and their parents plus two testers (Zhong 413 and IR64). For both BY and GY, there was significant inbreeding depression detected in the RI population and a high level of heterosis in each of the BC and testcross hybrid populations. The mean performance of the BC or testcross hybrids was largely determined by their heterosis measurements. The hybrid breakdown (part of inbreeding depression) values of individual RILs were negatively associated with the heterosis measurements of their BC or testcross hybrids, indicating the partial genetic overlap of genes causing hybrid breakdown and heterosis in rice. A large number of epistatic QTL pairs and a few main-effect QTL were identified, which were responsible for &gt;65% of the phenotypic variation of BY and GY in each of the populations with the former explaining a much greater portion of the variation. Two conclusions concerning the loci associated with inbreeding depression and heterosis in rice were reached from our results. First, most QTL associated with inbreeding depression and heterosis in rice appeared to be involved in epistasis. Second, most (~90%) QTL contributing to heterosis appeared to be overdominant. These observations tend to implicate epistasis and overdominance, rather than dominance, as the major genetic basis of heterosis in rice. The implications of our results in rice evolution and improvement are discussed.

scholarly journals Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress

2020 ◽  
Vol 21 (3) ◽  
pp. 772 ◽  
Author(s):  
Salah E. Abdel-Ghany ◽  
Fahad Ullah ◽  
Asa Ben-Hur ◽  
Anireddy S. N. Reddy
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transcriptome Analysis ◽  
Crop Yields ◽  
Seedling Stage ◽  
Limiting Factor ◽  
Drought Resistant ◽  
Sorghum Genotypes ◽  
Resistant Genotypes

Drought is a major limiting factor of crop yields. In response to drought, plants reprogram their gene expression, which ultimately regulates a multitude of biochemical and physiological processes. The timing of this reprogramming and the nature of the drought-regulated genes in different genotypes are thought to confer differential tolerance to drought stress. Sorghum is a highly drought-tolerant crop and has been increasingly used as a model cereal to identify genes that confer tolerance. Also, there is considerable natural variation in resistance to drought in different sorghum genotypes. Here, we evaluated drought resistance in four genotypes to polyethylene glycol (PEG)-induced drought stress at the seedling stage and performed transcriptome analysis in seedlings of sorghum genotypes that are either drought-resistant or drought-sensitive to identify drought-regulated changes in gene expression that are unique to drought-resistant genotypes of sorghum. Our analysis revealed that about 180 genes are differentially regulated in response to drought stress only in drought-resistant genotypes and most of these (over 70%) are up-regulated in response to drought. Among these, about 70 genes are novel with no known function and the remaining are transcription factors, signaling and stress-related proteins implicated in drought tolerance in other crops. This study revealed a set of drought-regulated genes, including many genes encoding uncharacterized proteins that are associated with drought tolerance at the seedling stage.

Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
F Nabbie ◽  
O Shperdheja ◽  
J Millot ◽  
J Lindberg ◽  
B Peethambaran
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Conditions ◽  
Knockout Mutants
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