EFFECTS OF TEMPERATURE AND OSMOTIC POTENTIAL ON GERMINATION OF SUNFLOWER AND SAFFLOWER AND ON HORMONE-TREATED SUNFLOWER SEEDS

1978 ◽  
Vol 58 (2) ◽  
pp. 295-301 ◽  
Author(s):  
M. MAFTOUN ◽  
A. R. SEPASKHAH
Keyword(s):  
Acetic Acid ◽  
Polyethylene Glycol ◽  
Carthamus Tinctorius ◽  
Naphthalene Acetic Acid ◽  
Sunflower Seeds ◽  
Salt Tolerant ◽  
Indole Butyric Acid ◽  
Effects Of Temperature ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

The influence of three temperatures and solutions of different osmotic potentials obtained with NaCl and/or polyethylene glycol 6000 was tested on the germination of sunflower (Helianthus annuus L.) and safflower (Carthamus tinctorius L.) seeds. The effects of two hormones at two concentrations on the germination of osmotically stressed sunflower seeds were also investigated. Seeds of the Record cultivar of sunflower were more salt-tolerant than Chernianka-66. Furthermore, Record germinated better at 20 °C than at 10° or 30 °C, while Chernianka-66 showed higher germination at 20° and 30 °C and was most sensitive to salinity at 10 °C. Seeds from Ute safflower exhibited more salt tolerance than did Local 3151, especially at the lower NaCl-induced osmotic potentials. The two safflower cultivars germinated better at 10° and 20 °C than at 30 °C. Seed germination of both crops was restricted more with polyethylene glycol 6000 than with NaCl, presumably due to the greater water stress caused by the former osmotic substrate. Germination of Record in NaCl substrate was inhibited at 50 ppm α-naphthalene acetic acid (NAA). NAA at 50 ppm decreased the germination of cv. Chernianka-66, whereas 3-indole butyric acid (IBA) at 10 ppm enhanced its germination. In general, germination of both sunflower cultivars was stimulated more by IBA than NAA. Furthermore, seeds treated with 10 ppm concentration gave better response than those treated with 50 ppm, possibly due to toxic effects.

scholarly journals Adaptive drought tolerance during germination of Salsola drummondii seeds from saline and nonsaline habitats of the arid Arabian deserts

Botany ◽  
2019 ◽  
Vol 97 (2) ◽  
pp. 123-133 ◽  
Author(s):  
Attiat Elnaggar ◽  
Ali El-Keblawy ◽  
Kareem A. Mosa ◽  
Teresa Navarro
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Drought Tolerance ◽  
Light Regimes ◽  
Saline Habitats ◽  
Population Origin ◽  
Effects Of Temperature ◽  
Osmotic Potentials

The effects of temperature, light, salinity, and drought on germination of halophytes have been extensively studied. However, few studies have focused on the germination of plants that grow well in both saline and nonsaline habitats (i.e., habitat-indifferent halophytes). Here, we assess the impacts of population origin, temperature, and light on drought tolerance, as simulated with polyethylene glycol (PEG), during germination of Salsola drummondii Ulbr., a habitat-indifferent halophyte from the arid Arabian deserts. Seeds were collected from both saline and nonsaline habitats and germinated at six concentrations of PEG at three temperatures and two light regimes. An increase in the concentration of PEG resulted in a significant reduction in seed germination, especially at higher temperatures. Seeds from the nonsaline habitat attained significantly greater germination efficiency at concentrations of PEG up to –1.2 MPa, but there was no difference in germination of seeds between the two habitats at concentrations of –1.5 MPa. Seeds from the saline habitat germinated significantly faster at higher concentrations of PEG. Germination was significantly higher in darkness than in light at –1.5 MPa at the lower temperatures, but the opposite was true for the higher temperatures. Seeds from saline habitats had higher levels of dormancy and faster rates of germination at higher concentrations of PEG because of their adaptation to low osmotic potentials.

scholarly journals Rooted cuttings by IBA (Indole butyric acid) and NAA (Naphthalene acetic acid) in black pepper (piper nigrum) - A case study in Vietnam

2020 ◽  
Vol 8 (3) ◽  
pp. 1880-1885
Author(s):  
Tran Quyen Nguyen ◽  
Ba Huy Nguyen ◽  
Dieu Hien Tran Thi ◽  
Oanh Duong Thi ◽  
Quang Ngoc Nguyen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Rooted Cuttings

scholarly journals Growth Response of Two Pepper (Piper nigrum L.) Stem Cuttings on Application of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid)

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Rizky Wulandari ◽  
Yaya Hasanah ◽  
Meiriani Meiriani
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Growth Response ◽  
Block Design ◽  
Primary Branch ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Secondary Branch ◽  
Indole Butyric Acid ◽  
Significant Difference

Using fruit branch for pepper shrub propagation is one of alternatives for an efficient pepper multiplication which usually uses underlayer cuttings. This research is aimed at finding the growth response of two pepper (Piper nigrum L.) cuttings to the administration of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid). This research was conducted at the greenhouse of the Faculty of Agriculture, University of Sumatera Utara, Medan (± 32 m above sea level), from April to August 2017 using a factorial randomized block design with 2 factors, pepper branch cuttings (primary branch cuttings and secondary branch cuttings) and the administration of IBA and NAA (0+0 ppm, 2500+0 ppm, 0+2500 ppm, 1500+1000 ppm, and 1000+1500 ppm). The results show that the emerging shoot rate in the secondary branch cuttings was significantly faster than in the primary branch cuttings, but the volume of root in the primary branch cuttings is significantly larger than in the secondary branch cuttings. There was no significant difference in the administration of IBA and NAA on all observed variables. The highest interaction of shoot length was found in the  primary branch cuttings with the administration of  IBA 1500 ppm + NAA 1000 ppm and the highest percentage of root and root volume was found in the primary branch cuttings with the administration of IBA 2500 ppm + NAA 0 ppm.

scholarly journals Factors Affecting Vegetative Propagation of Wintersweet (Chimonanthus praecox) by Softwood Cuttings

HortScience ◽  
2020 ◽  
Vol 55 (11) ◽  
pp. 1853-1860
Author(s):  
Huan Hu ◽  
Nan Chai ◽  
Haoxiang Zhu ◽  
Rui Li ◽  
Renwei Huang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Design Method ◽  
Orthogonal Design ◽  
Naphthalene Acetic Acid ◽  
Hormone Concentration ◽  
Range Analysis ◽  
Indole Butyric Acid ◽  
Factors Affecting ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox) is one of the most popular winter-blooming species. Effective vegetative propagation is necessary for commercial usage and protection of wintersweet. In the current study, the four factors, namely hormone type (A), hormone concentration (B), soaking duration (C), and medium (D), were assessed using an L16 (44) orthogonal test design. The hormone types include ABT (A1), α-naphthalene acetic acid (NAA) (A2), indole butyric acid (IBA) (A3), and indole-3-acetic acid (IAA) (A4); the hormone concentrations include 100 mg·L−1 (B1), 500 mg·L−1 (B2), 1000 mg·L−1 (B3), and 1500 mg·L−1 (B4); the soaking durations include 5 seconds (C1), 5 minutes (C2), 30 minutes (C3), and 3 hours (C4); and the mediums include perlite: peat in the ratios 1:0 (D1), 2:1 (D2), 1:1 (D3), and 1:2 (D4). The results showed that hormone and proper medium could significantly improve the cutting survival, rooting, and sprouting, whereas poor factor combinations, especially high hormone concentrations combined with long soaking durations may be threatened to the cuttings and rooting. In actual experiments, we successfully obtained an excellent rooting percentage (62.22%) of wintersweet from treatment No. 5 (A2B1C2D3), which is perlite and peat (1:1) as the medium and soaking the cuttings in 100 mg·L−1 NAA for 5 minutes as the hormone treatment. This combination can already meet the requirements for commercial production. A range analysis showed that the medium and hormone concentration were the most important factors affecting the cutting of wintersweet. An analysis of variance also showed that the medium and hormone concentration can significantly or extremely significantly affect most cutting indicators. Moreover, our results revealed that an orthogonal design method is an effective tool for establishing an improved technique for cutting propagation.

scholarly journals PENGARUH PEMBERIAN IBA (Indole Butyric Acid) DAN KONSENTRASI NAA (Naphthalene Acetic Acid) TERHADAP KEBERHASILAN PENYETEKAN SIRIH MERAH (Piper crocatum Ruiz and Pav.)

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Desi Maulida ◽  
Rugayah Rugayah ◽  
Tri Dewi Andalasari
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Block Design ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Randomized Block Design ◽  
The Difference ◽  
The Given

The research was conducted at the cutting red bettle (Piper crocatum Ruiz and Pav.) to determined the effect of (1) the difference growth of cuttings of red bettle which are given IBA and without IBA, (2) the concentration of NAA on growth of cutting red bettle, (3) the concentration of NAA on growth of cutting red bettle on each given IBA. The treatment was arranged in factorial (2 x 4) in randomized block design with three replication. The first factor were without IBA (A) and the given of 1000 ppm IBA (A1). The second factor were the concentration of NAA consists of: 0 ppm (B), 1000 ppm (B1), 2000 ppm (B2), and 4000 ppm (B). The results showed that the NAA concentration of 4000 ppm produced the most number of roots on either at the node or at the base of cutting. Planting cutting red bettle which given IBA 1000 ppm was able to accelerated the time leaves open and increased the number of cutting which germinate. The mixtured of giving IBA 1000 ppm and the concentration of NAA 4000 ppm, produced the most number of cutting that germinated.

scholarly journals Adventitious root formation in branch cuttings of Taxus wallichiana Zucc.(Himalayan yew): A clonal approach to conserve the scarce resource

2017 ◽  
Vol 8 ◽  
Author(s):  
Mohd Aslam ◽  
Parvaiz A. Raina ◽  
Rouf Ur Rafiq ◽  
T.O. Siddiqi ◽  
Zafar A. Reshi
Keyword(s):  
Acetic Acid ◽  
Adventitious Root ◽  
Root Formation ◽  
Block Design ◽  
Natural Habitat ◽  
Adventitious Root Formation ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Himalayan Yew ◽  
Taxus Wallichiana

Himalayan yew (<em>Taxus wallichiana</em> Zucc.), is an economically valuable plant and critically endangered due to overexploitation for the isolation of Taxol,an exciting anticancer drug from its bark and leaves. Since the species is unisexual and due to its long seed dormancy period and rapid loss of viability coupled with low survival percentage, its natural regeneration from seeds is very poor. As the seed raised plants add little growth, propagation by stem cuttings was tried under natural conditions and a considerable success was achieved after making use of different auxins (Indole-3- Acetic Acid-IAA, Indole Butyric Acid-IBA and Naphthalene Acetic Acid-NAA) in different concentrations. A Randomized block design (RBD) was adopted for laying the experiment of the present study. Of the ten treatments studied, IBA at 500ppm performed best of all the treatments and registered higher callusing percentage, rooting percentage, number of roots and length of roots in the juvenile shoot cuttings of the species. The results achieved through the application ofplant growth regulators (PGRs)by way of adventitious root formation (ARF) could be useful for the management of this understory coniferous tree species, whether for conservation, habitat restoration or for the production of Taxol,a promising anti-cancer agent. The technique evolved will be the most handy, quickest, inexpensive and can be applied any where in its natural habitat for the restoration and restocking of this valuable plant, which is otherwise facing the peril of extinction throughout the range of its distribution including Indian Himalayas.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals INFLUNCE OF AUXIN AND POLYAMINEES ON ROOTING OF SHOOTS OF CITRUS VOLKAMERIANA ROOTSTOCK IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Al- Khazali & Hamad
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Tissue Culture ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
College Of Agriculture ◽  
Indole Butyric Acid ◽  
Citrus Volkameriana

This  research  was  conducted  in  the  plant  tissue  culture  Lab. College  of Agriculture / University  of  Baghdad  from  February to  October  2015. The aim  of  the  study  was  investigating  the  influence  of  combinations  of Indole  butyric  acid (IBA) ,  Naphthalene  acetic  acid (NAA) and  polyamine Spermidine (Spd.) on rooting of shoots of  citrus volkameriana rootstock cultured  on 1\2  MS medium. The Results indicated that 1/2 MS medium supplemented  with 1.0 mg L-1 (IBA)  gave the highest  percentage  of  rooting  (67 %) which differed significantly  from the MS medium with free auxin IBA  that gave (22%) while  the  MS medium  supplemented with 0.5 mg L-1  spermidine  gave the highest percentage of rooting (63%) that was not significantly different  than other concentrations of Spd . MS medium supplemented with 1.0 mg L-1 IBA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (83%) and the highest root number / shoot (3.17) and highest length of root (3.15 cm) while the MS medium with free auxin IBA and spd. did not give percentage of rooting (0%) for citrus volkameriana rootstock plantlets . The MS medium supplemented with 1.0 mg L-1 NAA gave the highest percentage of rooting (56%) which differed significantly from MS medium with free auxin NAA that gave (22%)  while MS medium supplemented with 0.5mg L-1 spd. gave the highest percentage of rooting (50%) that was not significantly different from other concentration of Spd . MS medium supplemented with 1.0 mg L-1 NAA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (68%) and the highest root number /shoot (2.5) and highest length of root (2.65 cm) while the MS medium with free auxin NAA and Spd. did not gave percentage of rooting (0%) .

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