CRYOPRESERVATION OF APPLE GENETIC RESOURCES

1987 ◽  
Vol 67 (4) ◽  
pp. 1151-1154 ◽  
Author(s):  
C. STUSHNOFF
Keyword(s):  
Genetic Resources ◽  
Key Words ◽  
Gene Conservation ◽  
Cold Hardness ◽  
Key Words Cold

Key words: Cold hardness, gene conservation, Malus cryopreservation

EFFECTS OF PSYCHOLOGICAL STRESS, ACUTE COLD STRESS, AND DIET ON FORESTOMACH CONTRACTIONS IN CATTLE

1988 ◽  
Vol 68 (2) ◽  
pp. 399-407 ◽  
Author(s):  
A. LIRETTE ◽  
J. M. KELLY ◽  
L. P. MILLIGAN ◽  
R. J. CHRISTOPHERSON
Keyword(s):  
Heart Rate ◽  
Cold Stress ◽  
Key Words ◽  
Psychological Stress ◽  
Latin Square ◽  
Alfalfa Hay ◽  
Alfalfa Silage ◽  
Acute Psychological Stress ◽  
Key Words Cold

Studies were made on forestomach contraction frequencies as affected by diet (brome hay, alfalfa hay, alfalfa silage and barley concentrate diet), acute cold stress and acute psychological stress. Four 15-mo-old steers fitted with ruminal fistulae were used in a 4 × 4 Latin square design. Diets did not influence contractile frequencies of the forestomachs, and did not interact with the effects of the stresses. Acute cold stress and psychological stress both produced significant increases of forestomach contraction frequencies and of heart rate. Key words: Cold stress, psychological stress, forestomach contractions, heart rate

Cryopreservation of germplasm from chickens kept in Canadian research institutions

2008 ◽  
Vol 88 (4) ◽  
pp. 577-580 ◽  
Author(s):  
F. G. Silversides ◽  
Y. Song ◽  
R. Renema ◽  
B. R. Rathgeber ◽  
H. L. Classen
Keyword(s):  
Genetic Resources ◽  
Key Words ◽  
Agricultural Research ◽  
Genetic Material ◽  
Publicly Funded ◽  
Research Institutions ◽  
Cryogenic Storage ◽  
Canadian Research

Twenty-three genetically distinct lines of chickens are maintained as living populations by Agriculture and Agri-Food Canada and Canada’s eight Faculties of Agriculture. Historically, cryogenic storage of avian genetic material has been difficult, but we have developed techniques of gonadal transplantation to allow recuperation of stored genetic material into living birds. Gonads from 1660 day-old chicks or late-term embryos (810 females and 850 males) from 18 chicken populations from four Canadian institutions were harvested and cryopreserved using dimethylsulfoxide as a cryoprotectant. Future efforts will be directed to completing the collection of the populations kept in Canadian publicly-funded institutions that conduct agricultural research. Key words: Chicken, genetic resources, cryopreservation, gonads

Đánh giá một số dòng cà chua mới chọn tạo và khả năng tạo hạt lai của những dòng triển vọng tại Đà Lạt, Lâm Đồng/Evaluation of new tomato lines and capability of hybrid seed production of potential tomato lines at Dalat, Lamdong

2015 ◽  
Vol 108 (9) ◽  
Author(s):  
Nguyễn Đình Thi ◽  
Trần Đăng Hòa ◽  
Nguyễn Thế Nhuận ◽  
Phạm Thị Mùi
Keyword(s):  
Seed Germination ◽  
Genetic Resources ◽  
Key Words ◽  
Seed Production ◽  
Seed Vigor ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Agronomic Characters ◽  
Tomato Variety ◽  
Fruit Setting

Tóm tắt. Kết quả thí nghiệm của chúng tôi cho thấy: 1) 27 dòng cà chua thí nghiệm có các chỉ tiêu nông sinh học tương đối đa dạng, mức độ nhiễm sâu bệnh hại ít, chất lượng và tiềm năng cho năng suất quả cao. Đây là nguồn gen phong phú để lựa chọn sử dụng tạo giống lai; 2) Có 4 dòng có triển vọng và phù hợp với thị hiếu người tiêu dùng trong nước, các tính trạng tương đối ổn định là: 389, 485, 691 và 709; 3) Trong 12 tổ hợp lai (♀/♂) từ 4 dòng triển vọng, 4 tổ hợp có khả năng tạo hạt lai kém là: 691x709, 485x709, 709x691 và 389x691. 8 tổ hợp lai còn lại có các chỉ tiêu tỷ lệ đậu quả, số hạt/quả đạt chất lượng, số hạt lép/quả, khối lượng 1000 hạt, tỉ lệ nảy mầm của hạt lai đều đạt giá trị cao. 4) Cần tiếp tục so sánh con lai của 8 tổ hợp lai này với giống cà chua đang được sản xuất đại trà tại Lâm Đồng để đánh giá và tuyển chọn chúng trong những vụ tiếp theo. Từ khóa: dòng cà chua mới, hạt giống laiAbstract. The results of our research were indicated that: 1) 27 new tomato lines in our experiment have hightly variance in bio-agronomic characters, lack of pest infection, hight potential on quality and yield. They are good genetic resources for hybride seed breeding; 2) 4 potential tomato lines are 389, 485, 691 and 709; 3) in 12 crossing combination (♀/♂) for hybride seed breeding from 4 potential tomato lines, 4 crossing combination have bad in hybrid seed production (691x709, 485x709, 709x691 và 389x691), 8 crossing combination have good in fruit-setting, seeds number/fruit, 1.000 seeds weight, seed vigor and seed germination indicates. 4) Need to compare 8 crossing combination for hybrid seed breeding with commonalty tomato variety at Lam Dong for continue evaluation and collection in next seasons. Key words: new tomato lines, hybrid seed

scholarly journals Setting priorities for conservation of the conifer genetic resources of British Columbia

1996 ◽  
Vol 72 (4) ◽  
pp. 406-415 ◽  
Author(s):  
Alvin D. Yanchuk ◽  
Donald T. Lester
Keyword(s):  
British Columbia ◽  
Genetic Resources ◽  
Protected Areas ◽  
Natural Regeneration ◽  
Ex Situ ◽  
Gene Conservation ◽  
Conifer Species ◽  
The Future ◽  
Ex Situ Collections

Gene conservation of native conifer species in British Columbia is considered necessary primarily to safeguard the future evolutionary potential of species to climate change, new biotic challenges and for commercial genetic improvement programs. The tactical options include: (i) maintaining existing protected areas, (ii) creating new reserves for in situ management, and (iii) ex situ collections of various types.A two-part strategy is presented, with emphasis on technical justifications, for the conservation of conifer tree genetic resources of 23 British Columbia species. Part I is a survey of the frequency of each species in current land reserves in BC. Part II outlines an approach that will aid in setting priorities for additional gene conservation activities for specific species. This process attempts to use information on: (i) levels of in situ protection, (ii) the status of each species in current provenance research and breeding programs, and (iii) the relative capabilities for natural regeneration for each species.Representation of the 23 native conifer species in the current network of protected areas is generally complete. For instance, western hemlock is under little threat, as extensive ex situ collections are in field tests; it is well protected in the current reserve network, and it has a great capacity for natural regeneration. At the other extreme, whitebark pine has no ex situ collections made to date, needs additional protection in some ecoregions of the province, and generally has poor natural regeneration potential. While implementation of our strategy will be an ongoing process (i.e. updating information from both new and old in situ reserves, and setting new priorities among species), several immediate issues have been noted. These include; (i) follow-up work in the area of population sampling for ex situ collections, (ii) "ground truthing" of current reserves where data are weak, and (iii) examining the merit of certain populations not located in BC (as they could be as important as those currently protected in BC).For the future, various broad management questions will need to be resolved, such as: (i) whether existing reserves will perpetuate the various gene pools, and what management will be appropriate for such areas, (ii) the impact of pollen migration from genetically improved production stands on reserve stands, and (iii) the role of commercially improved stands and the breeding populations themselves to meet gene conservation objectives. Key words: gene conservation, native conifers, British Columbia, tree genetics

scholarly journals Tree species of concern in New Brunswick, Canada. II. Guidelines for conservation of genetic resources

2007 ◽  
Vol 83 (3) ◽  
pp. 402-407
Author(s):  
J A Loo ◽  
T L Beardmore ◽  
J D Simpson ◽  
D A McPhee
Keyword(s):  
New Brunswick ◽  
Genetic Resources ◽  
Tree Species ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
Gene Conservation ◽  
American Beech ◽  
Bur Oak ◽  
Butternut Canker

Guidelines were developed by the New Brunswick Forest Gene Conservation Working Group for conserving genetic resources of four native tree species in New Brunswick. Gene conservation guidelines for three of these species aim to maintain sufficient gene pools of known or putatively resistant stock to retain or develop the potential for restoration. Natural populations of American beech (Fagus grandifolia) are known to have genotypes resistant to the beech scale at low frequencies. Gene conservation approaches include in situ and ex situ measures, including resistance breeding. White elm (Ulmus americana) demonstrates a degree of resistance to Dutch elm disease (DED), and will benefit from a mixture of in situ and ex situ conservation measures. Resistance to butternut canker has not yet been demonstrated in populations of butternut (Juglans cinerea), but ex situ conservation of putatively resistant genotypes will be of increasing importance as the frequency of butternut canker escalates. Bur oak (Quercus macrocarpa), threatened by small population size, habitat loss, and ongoing development, requires primarily in situ conservation and restoration efforts. Key words: American beech, bur oak, butternut, disease resistance, ex situ, gene conservation guidelines, genetic resources, in situ, restoration, white elm

scholarly journals PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2017 ◽  
Vol 4 (1) ◽  
pp. 68
Author(s):  
M. H. Fariduddin Ath-thar ◽  
Intan Putriana ◽  
Dinar Tri Soelistyowati ◽  
Rudhy Gustiano
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Genetic Resources ◽  
Key Words ◽  
Genetic Relationship ◽  
Fragment Size ◽  
Random Amplified Polymorphic Dna ◽  
Tropical Species ◽  
Total Production ◽  
Culture Activity

Genotype performance of Tambakan, Helostoma temminckii (Cuvier, 1829) from Sumatera, Java and Kalimantan Polulation using Random Amplified Polymorphic DNA (RAPD)        Tambakan, kissing gourami (Helostoma temminckii) is well known as a freshwater tropical  species from Southeast Asia. In Indonesia, tambakan is an important commodity. However, total production of tambakan tends to decrease. Therefore, domestication has urgently to be done to solve the problem of Tambakan population. Three different tambakan populations from Sumatera, Java and Kalimantan were observed to find good genetic resources for culture activity. This study aimed to investigate the genetic variation of tambakan especially from West Java, Jambi, and South Kalimantan province using RAPD. The result showed that the highest polymorphism and heterozygosity was from South Kalimantan population among others. The three population observed had the fragment size ranged from 100- 2000 bp. The highest genetic distance was between Sumatera and Kalimantan (0,2877), while the lowest was between Kalimantan and Java (0,1961). Key words: Helostoma temminckii, genetic, heterozigosity, genetic relationship ABSTRAK        Ikan tambakan (Helostoma temminckii) adalah salah satu jenis ikan air tawar yang berasal dari wilayah tropis, tepatnya Asia Tenggara. Di Indonesia ikan tambakan merupakan salah satu ikan komoditas penting. Saat ini, jumlah produksi ikan tambakan cenderung menurun. Sehingga domestikasi sangat diperlukan untuk mengatasi masalah peneurunan populasi tambakan Tiga populasi ikan tambakan dari Sumatera, Jawa dan Kalimantan diobeservasi untuk mendapatkan sumber genetic terbaik yang dapat digunakan untuk kegiatan budidaya.. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetika populasi ikan tambakan Jambi, Jawa Barat dan Kalimantan Selatan menggunakan metode RAPD. Hasil menunjukkan bahwa polimorfisme dan heterosigositas tertinggi terdapat pada populasi ikan tambakan Kalimantan Selatan jika dibandingkan dengan populasi ikan tambakan lainnya. Ukuran fragmen DNA teramplifikasi berkisar antara 100-2000 bp. Jarak genetik paling jauh adalah antara populasi tambakan Sumatera dengan Kalimantan (0,2877), sedangkan jarak genetik terendah adalah tambakan Kalimantan dengan Jawa (0,1961).Kata kunci: Helostoma temminckii, genetik, heterosigositas, kekerabatan

Evaluation of sheep genetic resources in North America: Lamb productivity of purebred, crossbred and synthetic populations

2008 ◽  
Vol 88 (3) ◽  
pp. 391-398 ◽  
Author(s):  
J. N. B. Shrestha ◽  
W. J. Boylan ◽  
W. E. Rempel
Keyword(s):  
North America ◽  
Genetic Resources ◽  
Key Words ◽  
North American ◽  
Reciprocal Crosses ◽  
Key Words Growth ◽  
Genetic Types ◽  
Synthetic Populations ◽  
Breed Crosses

Lamb weights and daily gains from divergent genetic types of established purebreds, e.g., Dorset (D), Lincoln (L), Rambouillet (Ra), Suffolk (Su) and Targhee (T), and fecund-type breeds, e.g., Finnsheep (F) and Romanov (Ro), their reciprocal crosses and Suffolk sired specific cross Su(F × Ro) were evaluated. Also evaluated were lambs of the Outaouais (O) and Rideau (R) Arcott breeds and their reciprocal crosses, in addition to Synthetic I (½ F, ½ L), Synthetic II (½ D, ½ Ra) and Synthetic III (¼ F, ¼ L, ¼ D, ¼ Ra) populations. The established purebreds produced heavier lambs at birth and weaning, Arcott breed crosses gained weight more rapidly resulting in heavier lambs at 140 d, and fecund-type breeds produced lighter lambs (P < 0.05). In general, daily gains and lamb weights of all genetic types were similar, except that fecund-type breeds produced significantly lighter lambs. Lamb weights of T were most at birth, and Su at weaning and 140 d, while F lambs weighed the least (P < 0.05). Within established purebreds, Su weighed the most and D weighed the least, while L, Ra and T lambs were intermediate. Daily gains including weaning and 140-d weights of F and R cross lambs benefited from 7–9% heterosis, while the Arcott breed cross lambs not only benefited from 5–8% heterosis, but were comparable with Su lambs. At the same time, lamb performance of Su(F × Ro) was similar to the average of their parental breeds. Lambs of synthetic populations relative to the average of their respective parental breeds weighed 8–24% more at 140 d, suggesting heterosis retention. Key words: Growth, North American breeds, Finnsheep, Romanov, Arcotts, synthetic populations

ASSESSMENT AND CONSERVATION OF ANIMAL GENETIC RESOURCES IN CANADA

1984 ◽  
Vol 64 (2) ◽  
pp. 235-251 ◽  
Author(s):  
R. D. CRAWFORD
Keyword(s):  
Dairy Cattle ◽  
Genetic Resources ◽  
Key Words ◽  
Public Agencies ◽  
Conservation Assessment ◽  
Conservation Action ◽  
Livestock Species ◽  
Animal Genetic Resources ◽  
Key Words Animal ◽  
Resources Conservation

An assessment is made of the present Canadian situation regarding livestock and poultry genetic resources and of the need to take conservation measures as insurance against future needs. Loss of resources in poultry has been extreme, and loss is occurring rapidly now in dairy cattle. A complete inventory of poultry genetic resources kept by commercial breeders, hobbyists, and public agencies was prepared in 1979–1980; it is described in detail. There is an annual inventory of research stocks, both mammalian and avian. A brief survey has been made of rare and feral livestock. There is an urgent need for complete cataloguing of existing resources in the major livestock species — cattle, sheep and swine. Three conservation collections of poultry stocks are located in Canada. Conservation action is also needed for livestock, especially cattle and swine. Canadians have a duty to maintain their animal genetic resources. While commercial animal breeders and hobbyists have a role to play in conservation, the main responsibility should be assumed by public agencies. Key words: Animal genetic resources, conservation, assessment, livestock, poultry

scholarly journals KERAGAMAN GENETIK KELAPA DALAM BALI (DBI) DAN DALAM SAWARNA (DSA) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2020 ◽  
Vol 16 (2) ◽  
pp. 83
Author(s):  
DONATA S. PANDIN
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Key Words ◽  
Genetic Relationship ◽  
Banding Pattern ◽  
Numerical Taxonomy ◽  
Random Amplified Polymorphic Dna ◽  
Population Based ◽  
Plant Biology ◽  
Biology Laboratory

<p>ABSTRACT</p><p>Keragaman genetik dan hubungan kekerabatan dalam populasikelapa Dalam Bali (DBI) dan Dalam Sawarna (DSA) dianalisismenggunakan penanda RAPD. Penelitian dilaksanakan di LaboratoriumBiologi Tumbuhan, Pusat Penelitian Sumber Daya Hayati danBioteknologi, Institut Pertanian Bogor pada Februari-Mei 2007. Bahanyang digunakan dalam penelitian sebanyak 10 individu dari masing-masing populasi. Primer acak digunakan dalam analisis terdiri atas 10primer -10 mer yaitu OPA-02, OPA-08, OPA-10, OPA-13, OPA-20,OPB-08, OPB-11, OPB-12, OPB-15, OPB-20. DNA diekstraksimenggunakan metode Rohde yang telah dimodifikasi, konsentrasiditetapkan menggunakan metode Sambrook. Untuk melihat tingkatkekerabatan antar individu berdasarkan pola pita RAPD dari setiap primerdigunakan program NTsys ver. 2,0 (Program Numerical Taxonomy andMultivariate Analysis), sedangkan untuk analisis gerombol digunakanmetode UPGMA untuk membuat dendogram. Koefisien keragaman antarindividu dalam populasi kelapa DBI berkisar antara 2,4% – 30,7% denganrata-rata 21,7%, dan untuk populasi kelapa DSA antara 1,5% – 22,4%dengan rata-rata 12,7%. Jarak genetik individu-individu dalam populasikelapa Dalam Bali (DBI) cukup jauh menunjukkan bahwa keragamangenetik dalam populasi Dalam Bali masih tinggi, sehingga seleksi untukmaksud perbaikan sifat masih sangat memungkinkan. Pada populasikelapa Dalam Sawarna (DSA) jarak genetik individu-individu dalam sudahsemakin sempit, artinya keanekaragaman genetik antar individu di dalampopulasi DSA sudah sangat rendah oleh karena itu seleksi untuk maksudperbaikan sifat harus dilakukan dengan selektif. Hubungan kekerabatanantar populasi kelapa Dalam Bali dan Dalam Sawarna sebesar 44% artinyajarak genetik kedua populasi ini cukup jauh yaitu 56%. Sehingga jikaindividu-individu terseleksi dari kedua populasi tersebut disilangkan, akandiperoleh keturunan yang memilikinilai heterosis tinggi.</p><p>Kata kunci: Kelapa Dalam Bali, kelapa Dalam Sawarna, keragamangenetik, hubungan kekerabatan, RAPD</p><p>ABSTRACT</p><p>Genetic Diversity of Bali Tall (DBI) and Sawarna Tall(DSA) Coconuts Based  on  Random  AmplifiedPolymorphic DNA (RAPD</p><p>)Genetic Diversity of Bali Tall (DBI) and Sawarna Tall (DSA)coconuts based on Random Amplified Polymorphic DNA (RAPD) wasobserved. Ten plants were used in each population. The objectives of thisresearch were to determine genetic diversity within-and inter-population ofBali Tall (DBI) and Sawarna Tall (DSA) coconuts, and geneticrelationship of those population based on RAPD (Random AmplifiedPolymorphic DNA). Research was done in Plant Biology Laboratory ofCenter Research of Genetic Resources and Biotechnology, InstitutPertanian Bogor, February – May 2007. DNA extraction was done bymodified Rohde method and to determine the concentration and quality ofDNA by Sambrook method. Ten RAPD 10-mer were used namely OPA-02, OPA-08, OPA-10, OPA-13, OPA-20, OPB-08, OPB-11, OPB-12,OPB-15, OPB-20. To find out the level of genetic relationship betweenindividuals based on RAPD banding pattern of each primer, we usedNTsys program ver. 2.0 (program Numerical Taxonomy and MultivariateAnalysis System), whereas for the analysis of clustering UPGMA methodis used to create a dendogram. These ten RAPD primers could separateDBI and DSA in each group. Genetic diversity within-population of BaliTall coconut population varied from 2.4 to 30.7% with average of 21.7%.So that, opportunity to improve characters in DBI coconut populationcould be done by selection. Genetic diversity within-population ofSawarna Tall coconut population progressively was narrow, ranging from1.5 to 12.4% with average 12.7%, so the selection in order to do characterimprovement in this population could be done selectively. Geneticrelationship between DBI dan DSA populations was far enough (54%), sothe crossing between those population will be good for charactersimprovment.</p><p>Key words : Bali Tall coconut, Sawarna Tall coconut, genetic diversity,genetic relationship, RAPD</p>

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