Authentification des 13 cultivars de fraisier du programme de certification du Québec par l'analyse d'ADN polymorphe amplifié au hasard (RAPD)

1995 ◽  
Vol 75 (1) ◽  
pp. 221-224 ◽  
Author(s):  
Jean-Guy Parent ◽  
Danièle Pagé
Keyword(s):  
Key Words ◽  
Dna Analysis ◽  
Cultivar Identification ◽  
Random Amplified Polymorphic Dna ◽  
Certification Program ◽  
Strawberry Cultivars ◽  
Selection Of ◽  
Apparently Healthy

Random amplified polymorphic DNA analysis was used to identify and differentiate the 13 strawberry cultivars of Quebec's certification program. DNA extracts from leaves of field plants were analyzed. Young and apparently healthy leaves were chosen to ensure a good quality of DNA. A selection of only two primers, in a test combination, was sufficient to attain the anticipated result. Key words: Strawberry (Fragaria spp.), random amplified polymorphic DNA, RAPD, cultivar identification

Identification de cultivars de framboisier par l’analyse d’ADN polymorphe amplifié au hasard (RAPD)

1993 ◽  
Vol 73 (4) ◽  
pp. 1115-1122 ◽  
Author(s):  
Jean-Guy Parent ◽  
Danièle Pagé ◽  
Marc G. Fortin
Keyword(s):  
Key Words ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Simple Test ◽  
Certification Program ◽  
Growth Season ◽  
Plant Dna ◽  
In Vitro Plantlets

We developed a fast and simple test to accurately characterize the various cultivars of raspberry of the Quebec certification program. This test, based on random amplified polymorphic DNA (RAPD) analysis, uses minute quantities of plant DNA. Portions of this DNA are multiplied with small 10-nucleotide primers of arbitrary sequences. Analyses were done on DNA from 15 raspberry cultivars amplified with 19 different primers. A combination of three primers was chosen for its ability to differenciate all the cultivars. Test reproducibility was checked with DNA samples from plants collected at various periods of the growth season and from plants of various categories of the certification program (in vitro plantlets, Foundation and Certified plants, commercial plants). Key words: Raspberry (Rubus spp.), random amplified polymorphic DNA, cultivar identification

scholarly journals AN ATTEMPT TO ESTIMATE TEAT QUALITY OF SOWS BY HAND MILKING DURING FARROWING

1984 ◽  
Vol 64 (1) ◽  
pp. 165-170 ◽  
Author(s):  
D. FRASER ◽  
C. S. LIN
Keyword(s):  
Key Words ◽  
Strong Tendency ◽  
Teat Number ◽  
Selection Of

For 21 sows, teats were hand milked individually in a standard way during farrowing in an attempt to produce an index of teat quality. The piglets' teat selection and 14-day weights were then compared with the hand milking results. Of within-litter variation in 14-day weight, 38.6% was explained by 1-day weight, and only 4.6% extra variation by the index derived from hand milking. Use of the index gave no improvement over previous models involving 1-day weight and teat number (anterior to posterior). Hand milking showed a pronounced decline in colostrum yield from the most anterior teat pair (46.8 g) to the most posterior (13.7 g); this may help to explain the piglets' strong tendency to select anterior positions. Piglets of high, medium and low 1-day weight differed significantly in liveweight gain, but not in their selection of anterior or posterior teats. Key words: Colostrum, milk, piglet, sow, suckling behavior, teat quality

scholarly journals Characterizing the Sphaceloma Fungus, Causal Agent of Superelongation Disease in Cassava

Plant Disease ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 423-428 ◽  
Author(s):  
Elizabeth Alvarez ◽  
Martha L. Molina
Keyword(s):  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Management Strategies ◽  
Restriction Enzymes ◽  
Colony Growth ◽  
Molecular Characteristics ◽  
Intermediate Reaction ◽  
Polymerase Chain ◽  
The Tropics ◽  
Selection Of

The fungus Sphaceloma manihoticola causes superelongation disease in cassava, a starchy root crop grown widely in the tropics. Isolates were collected from infected plants grown in six localities of Colombia. Morphological analyses of the fungus showed that colony growth and color are not stable characteristics over time. Pathogenicity studies, using the susceptible cassava variety M Col 22 and the resistant M Ven 77, showed that M Col 22 was tolerant of 29% of pathogen isolates studied and had an intermediate reaction to 71%. Variety M Ven 77 also showed tolerance of 16.2% of the isolates, had an intermediate reaction to 80.6%, and was susceptible to 3.2%. Significant cultivar × isolate interactions indicated pathogenic specialization. This study is the first to describe this pathogen's molecular characteristics. A homogeneous and reporducible band of about 545 bp was obtained with polymerase chain reaction which, when digested by restriction enzymes, showed an equal pattern of bands for all isolates. The isolates thus belonged to one species. Random amplified polymorphic DNA analysis revealed intraspecific genetic diversity. By better understanding the pathogen, we can apply more appropriate disease management strategies, such as selection of germ plasm tolerant of superelongation disease.

Genetic diversity in sunflower (Helianthus annuus L.) as revealed by random amplified polymorphic DNA analysis

1994 ◽  
Vol 45 (7) ◽  
pp. 1319 ◽  
Author(s):  
WR Lawson ◽  
RJ Henry ◽  
JK Kochman ◽  
GA Kong
Keyword(s):  
Genetic Diversity ◽  
Ribosomal Rna ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Efficient Tool ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Sunflower Genome ◽  
Pair Wise Comparison ◽  
Selection Of

A cross-section of sunflower genotypes grown in Australia including commercial cultivars (Suncross 40R, Hysun 33, Hysun 45CQ, Advance, DK3873), breeding lines (Sunfola, S37- 388, PhRR3, HA-R2, MC29, MC69, S37-388RR), wild sunflower varieties (H. annuus, H. argophyllus), a distantly related species (Tithonia diversifolia), and a hexaploid/diploid cross (H. tuberosus L.x H. annuus L.) were assessed for genetic diversity using RAPD (Random Amplified Polymorphic DNA) analysis. A considerable amount of polymorphism was revealed. Of the total of 158 markers amplified, 133 were polymorphic for at least one pair-wise comparison within the 16 genotypes. Overall, 33% dissimilarity was detected, with an average of 27% dissimilarity revealed among the hybrids and breeding lines, which exhibited 38% dissimilarity to the wild varieties H. annuus and H. tuberosus, and 51% dissimilarity to Tithonia and H. tuberosus x H, annuus. PCR of the 5S ribosomal RNA gene spacer region did not reveal any polymorphisms among the cultivated and breeding lines, but did distinguish between H. tuberosus and the other wild species. This survey of a selection of sunflower genotypes indicates that the genetic base of domesticated sunflower may be quite wide. These results suggest that RAPD methodology will provide an efficient tool for the analysis of the sunflower genome, in particular in breeding programs.

scholarly journals Identification of the Edible Fig `Bianco del Cilento' by Random Amplified Polymorphic DNA Analysis

HortScience ◽  
1999 ◽  
Vol 34 (7) ◽  
pp. 1263-1265 ◽  
Author(s):  
U. Galderisi ◽  
M. Cipollaro ◽  
G. Di Bernardo ◽  
L. De Masi ◽  
G. Galano ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Southern Italy ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Accurate Identification ◽  
Banding Patterns ◽  
Campania Region ◽  
Quality Of Products

Random amplified polymorphic DNA (RAPD) analysis is currently used to estimate genetic relationships in plants. We have used RAPD analysis to distinguish six different cultivars of Ficus carica, and several of their clones, that are widespread in the Campania Region of Southern Italy. Among these cultivars, `Bianco del Cilento' has unique characteristics, and is particularly useful for drying and for the manufacture of syrups. The protection of this cultivar is important to the Campania Region. We have utilized molecular markers to allow accurate identification of this cultivar, making it possible to control the quality of products and prevent fraudulent commerce. DNA was extracted from leaves and amplified by PCR using random oligonucleotide primers. The amplification patterns obtained with five decamer primers were useful for distinguishing all six cultivars analyzed. `Bianco del Cilento' was identified by two primers. The banding patterns were scored and used in similarity value calculations to estimate genetic relationships.

scholarly journals Identification of Raspberry Cultivars by Sequence Characterized Amplified Region DNA Analysis

HortScience ◽  
1998 ◽  
Vol 33 (1) ◽  
pp. 140-142 ◽  
Author(s):  
Jean-Guy Parent ◽  
Danièle Pagé
Keyword(s):  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Rubus Idaeus ◽  
Scar Markers ◽  
Certification Program ◽  
Red Raspberry ◽  
Sequence Characterized Amplified Region

Five polymorphic random amplified polymorphic DNA (RAPD) markers for 13 red raspberry (Rubus idaeus L.) and two purple raspberry (R. idaeus L. × R. occidentalis L.) cultivars were cloned and their termini sequenced. Sequence-specific 24-mer primer pairs were synthesized as extended RAPD primers and used in sequence characterized amplified region (SCAR) DNA analysis. All primer pairs generated polymorphic SCAR markers of the original RAPD marker sizes and length variants. Markers from four of the primer pairs could be easily scored and were adequate to identify the raspberry cultivars of the certification program of the province of Quebec.

scholarly journals Description of three new arctic bramble cultivars and proposal for cultivar identification

1998 ◽  
Vol 7 (4) ◽  
pp. 455-468 ◽  
Author(s):  
H. PIRINEN ◽  
P. DALMAN ◽  
S. KÄRENLAMPI
Keyword(s):  
Dna Analysis ◽  
Cultivar Identification ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characteristics ◽  
Growing Season ◽  
The Arctic ◽  
New Cultivars

Morphological and yield differences between five arctic bramble (Rubus arcticus L.) strains and the cultivars Pima and Mespi were studied at two locations during the growing season 1994. Morphological observations and measurements were made on canes, leaves, flowers and berries, adapting the UPOV (Union pour la Protection des Obtentions Vegetales) descriptions for strawberry and raspberry. The plants were also compared with the aid of random amplified polymorphic DNA analysis. Apart from DNA, the most important distinctive characteristic was yield. Characteristics of the flowers, leaves and berries, such as number, size, shape and colour were used to differentiate between the arctic bramble genotypes. The yield of three new cultivars, named Marika, Muuruska and Elpee, was greater than that of Pima or Mespi. The yield of 'Marika' and 'Elpee' was 2,4-times and that of 'Muuruska' 1,9-times that of 'Pima' or 'Mespi'. Based on distinctive morphological characteristics, guidelines for the description of arctic bramble are proposed. This description may also be used for hybrids of arctic bramble with Rubus stellatus Sm., i.e. Rubus arcticus L. nothosubsp. stellarcticus G. Larsson. ;

Analysis of Heterodera avenae populations by the random amplified polymorphic DNA technique

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 118-122 ◽  
Author(s):  
I. López-Braña ◽  
A. Delibes ◽  
M. D. Romero
Keyword(s):  
Cluster Analysis ◽  
Key Words ◽  
Dna Analysis ◽  
Fragment Size ◽  
Random Amplified Polymorphic Dna ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Heterodera Avenae ◽  
Random Primers

Eleven populations of the Heterodera avenae complex (four Spanish, two British, two French, and three Swedish) were studied by random amplified polymorphic DNA analysis. From 5 to 11 fragments were obtained with each of 14 random primers, with fragment size ranging from 200 to 2200 bp. Cluster analysis of the 11 populations, using 108 scorable markers, separate these populations into two main groups. These groups coincide with what is known as the "true" H. avenae and the "Gotland strain" or "British pathotype 3" of H. avenae. The results also clarify the relationships among some members of the H. avenae complex established previously using morphological and biochemical criteria. Key words : cereal cyst nematode, CCN, Heterodera avenae, RAPD.

scholarly journals Conceptual model of organizational and economic mechanism of mobilization of resource potential of the trade enterprise

2020 ◽  
pp. 59-64
Author(s):  
Serhii Zubkov
Keyword(s):  
Key Words ◽  
Conceptual Model ◽  
Financial Crises ◽  
Resource Potential ◽  
Economic Mechanism ◽  
Constituent Elements ◽  
Enterprise Analysis ◽  
Selection Of ◽  
Model Enterprise

Purpose. The aim of the article is to substantiate the constituent elements of the organizational and economic mechanism of mobilization of the resource potential of the enterprise. Methodology of research. The following methods are used in the article, namely: monographic – to study the works and substantiate own interpretation of the concept of organizational and economic mechanism of mobilization of resource potential of the enterprise; systematization and generalization – selection of components of the organizational and economic mechanism of mobilization of resource potential of the enterprise; analysis and modelling – development of a model of organizational and economic mechanism for mobilizing the resource potential of the enterprise. Findings. The author's interpretation of the concept “organizational and economic mechanism of mobilization of resource potential of the enterprise” is presented. The model of the organizational and economic mechanism of mobilization of resource potential of the enterprise is developed and the constituent elements of the organizational and economic mechanism of mobilization of resource potential of the enterprise are substantiated. It is proved that the construction of organizational and economic mechanism of mobilization of resource potential of the enterprise should combine a significant set of elements, the quality of formation and application of which depends on the effectiveness of measures to mobilize various elements of resource potential, which, of course, should affect the efficiency of the enterprise, especially in economic and financial crises. Originality. A conceptual model of the organizational and economic mechanism to ensure the mobilization of the resource potential of the trade enterprise, which includes the purpose and objectives, principles, subjects and objects of mobilization; support subsystems, functional, target; methods, ways and tools of resource potential mobilization. Practical value. The obtained results can be used in the activities of trade enterprises during the formation of the system of mobilization of resource potential to increase the efficiency of its use. Key words: model, enterprise, mechanism, organizational and economic mechanism, resource potential.

Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysis

Euphytica ◽  
1995 ◽  
Vol 92 (3) ◽  
pp. 341-351 ◽  
Author(s):  
H. N. Asemota ◽  
J. Ramser ◽  
C. Lop�z-Peralta ◽  
K. Weising ◽  
G. Kahl
Keyword(s):  
Genetic Variation ◽  
Dna Analysis ◽  
Cultivar Identification ◽  
Random Amplified Polymorphic Dna
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