FREEZE-THAW EFFECTS ON GRANULAR STRUCTURE REORGANIZATION FOR SOIL MATERIALS OF VARYING TEXTURE AND MOISTURE CONTENT

1988 ◽  
Vol 68 (3) ◽  
pp. 485-494 ◽  
Author(s):  
S. PAWLUK
Keyword(s):  
Moisture Content ◽  
Lacustrine Sediments ◽  
Field Capacity ◽  
Granular Structure ◽  
Glacial Till ◽  
Microstructural Development ◽  
Clay Loam ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Repeated Freezing

Repeated freezing and thawing of glacial till cores of clay loam texture results in the formation of granic and metafragmic microfabrics. These units of fabric are best developed near the surface of cores kept at moisture levels between field capacity and saturation. Well-sorted lacustrine sediments with fewer voids tend to form banded fabrics. Many of the morphological features such as vesicles, metavughs and desiccation cracks commonly attributed to freeze-thaw processes are evident in all materials tested. Discrete units of fabric observed in this study are very similar to units of fabric observed in the Ah horizons of Black Chernozemic and Cryosolic soils. Results of this investigation strongly support earlier research which suggests that frost processes are major contributors to their microstructural development. Key words: Granic, freeze-thaw, microfabrics

Inactivation of a Cold-Induced Putative RNA Helicase Gene of Listeria monocytogenes Is Accompanied by Failure To Grow at Low Temperatures but Does Not Affect Freeze-Thaw Tolerance

2010 ◽  
Vol 73 (8) ◽  
pp. 1474-1479 ◽  
Author(s):  
REHA O. AZIZOGLU ◽  
S. KATHARIOU
Keyword(s):  
Listeria Monocytogenes ◽  
Normal Growth ◽  
Rna Helicase ◽  
Soft Agar ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Transposon Insertion ◽  
Helicase Gene ◽  
Repeated Freezing ◽  
Cold Sensitive

Freeze-thaw tolerance (cryotolerance) of Listeria monocytogenes is markedly influenced by temperature of growth of the bacteria, and may involve responses to low-temperature stresses encountered during freezing and thawing. A cold-sensitive mariner-based transposon mutant of L. monocytogenes F2365 was found to harbor a single insertion in LMOf2365_1746, encoding a putative RNA helicase, and earlier shown by other investigators to be induced during 4°C growth of L. monocytogenes. The mutant had normal growth at 37°C but completely failed to grow at either 4 or 10°C, and had impaired growth and reduced swarming on soft agar at 25°C. However, the mutation had no discernible influence on the ability of the bacteria to tolerate repeated freezing and thawing after growth at either 25 or 37°C. The findings suggest that the transposon insertion in the putative helicase gene, in spite of the severely cold-sensitive phenotype that accompanies it, does not affect the ability of the bacteria to cope with cold-related stresses encountered during repeated freezing and thawing.

Freeze/thaw stability of transferrin, and reference values obtained with kinetic nephelometry.

1984 ◽  
Vol 30 (1) ◽  
pp. 114-115 ◽  
Author(s):  
L Stoddard ◽  
W Dennis ◽  
R M Parvin ◽  
O W van Assendelft
Keyword(s):  
Reference Values ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Normal Reference ◽  
Repeated Freezing ◽  
Blood Specimens ◽  
Normal Adults

Abstract To determine whether transferrin is stable to repeated freezing and thawing, we used blood specimens from 100 normal adults. Their transferrin concentrations were determined by a laser kinetic nephelometric immunoassay. The sera were stored at 4 degrees C and analyzed the next day (day 1). Specimens 1-25 were stored at -20 degrees C and assayed again on days 3, 4, 8, 16, 22, and 43. Specimens 26-100 were stored at 4 degrees C and analyzed again on day 2. Transferrin proved to be stable to repeated freezing and thawing. Normal reference values with respect to sex and age are given, as determined with the Baker 420 Immunological Nephelometer.

Effects of cyclic freezing and thawing on volume changes and permeabilities of soft fine-gained soils

1996 ◽  
Vol 33 (4) ◽  
pp. 529-537 ◽  
Author(s):  
K D Eigenbrod
Keyword(s):  
Moisture Content ◽  
Initial Moisture Content ◽  
Maximum Volume ◽  
Freezing And Thawing ◽  
Volume Changes ◽  
One Dimensional ◽  
Freeze Thaw ◽  
Fine Grained ◽  
Liquidity Index ◽  
Thaw Consolidation

Soft, fine-grained soils were exposed to cyclic one-dimensional, open-system freezing and thawing, resulting in maximum volume changes of up to 30%, depending on the initial moisture content and plasticity of the clay as well as on the rate of freezing. A linear relationship between the net volume changes subsequent to freezing and thawing and the liquidity index prior to freezing and thawing was obtained. This correlation is not unique, but depends on rate and mode of freezing. Thus, settlements from freeze–thaw consolidation in the field can be predicted from such tests if the rate and mode of freezing are the same as in the field. During cyclic freezing and thawing the soils became fissured and jointed, resulting for most clays in large increases in their bulk permeabilities, which increased with an increasing number of freeze–thaw cycles, often by more than two orders of magnitude. For some materials, however, little change in permeability occured. Key words: cyclic freeze–thaw, clays, freeze–thaw consolidation, permeability, volume changes.

scholarly journals Glacial and Periglacial Transformation of Palaeokarst in the Lublin-Volhynia Region (Se Poland, NW Ukraine) on the Base of TL Dating

2007 ◽  
Vol 27 (-1) ◽  
pp. 41-46 ◽  
Author(s):  
Radosław Dobrowolski ◽  
Stanisław Fedorowicz
Keyword(s):  
Genetic Relationship ◽  
Experimental Investigations ◽  
Considerable Influence ◽  
Freezing And Thawing ◽  
Tl Dating ◽  
Freeze Thaw ◽  
Se Poland ◽  
Repeated Freezing ◽  
Formation Conditions ◽  
Close Genetic Relationship

Glacial and Periglacial Transformation of Palaeokarst in the Lublin-Volhynia Region (Se Poland, NW Ukraine) on the Base of TL Dating Distinctly diverse results of TL dating are obtained for the deposits with similar lithofacial features but filling morphologically differentiated karst palaeoforms (dolines, pipes, pockets). The infillings of dolines and pipes are mostly of the Saalian age. Based on sedimentological analysis, their formation conditions are related to sub- or/and terminoglacial environment. The age obtained for all infillings of pockets is underestimated in comparison with lithostratigraphic data. A close genetic relationship between these forms and periglacial conditions seems to indicate that the reduction of TL signal is mostly influenced by the disintegration of grains resulted from the repeated freezing and thawing of glaciogenic deposits. A considerable influence of frost weathering on the decrease of thermoluminescence intensity of mineral grains is indirectly confirmed by the results of experimental investigations consisting in the repeated TL measurements of pockets' infillings after successive freeze-thaw cycles.

scholarly journals PSIV-25 Effect of Various Handling Methods of Plasma and Serum Samples on Pregnancy Associated Glycoprotein Concentrations

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 289-290
Author(s):  
Grace M Wesson ◽  
Lohana Fernandez ◽  
Rebecca K Poole ◽  
Gessica A Franco ◽  
Sydney T Reese ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Plasma Samples ◽  
Freezing And Thawing ◽  
Serum Samples ◽  
Freeze Thaw ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Repeated Freezing ◽  
Pregnancy Status ◽  
Handling Methods

Abstract Pregnancy associated glycoproteins (PAG) can be used as a biomarker for early pregnancy diagnosis, so accurate and consistent PAG detection is critical. The objective of this study was to determine if plasma and serum PAG concentrations were altered when centrifugation occurred at different times post-collection, when subjected to repeated freezing and thawing, and when monoclonal antibodies were kept in frequently or infrequently opened containers. Plasma (n = 4) and serum (n = 4) samples were collected from two open cows and two pregnant cows 28 days after artificial insemination. Pregnancy status was determined via transrectal ultrasonography. Plasma and serum samples were evenly separated and either centrifuged on the day of collection, or placed at 4°C and centrifuged the next day. An in-house PAG ELISA was performed on all samples before freezing (NOTHAW), after being frozen for one week (INTACT), after one freeze/thaw cycle (THAW1), two freeze/thaw cycles (THAW2), and three freeze/thaw cycles (THAW3). Data were analyzed using one-way ANOVA (GLM procedure, SAS 9.4). All samples from open cows were below the baseline of the assay. For pregnant cows, plasma samples had greater PAG concentrations than serum samples (11.84 vs 3.30 ± 0.66 ng/mL, respectively, P < 0.05). No differences were observed for day of centrifugation in both plasma and serum samples (P = 0.50 and P = 0.60, respectively) and in handling of monoclonal antibodies (P = 0.90). Freezing and thawing did not impact PAG concentrations in plasma samples (P = 0.19), but did alter serum concentrations (P = 0.01). Specifically, THAW1 (1.98 ng/mL) and THAW2 (1.42 ng/mL) serum PAG concentrations were lower compared to NOTHAW, THAW3, and INTACT samples (4.66, 4.85, and 3.57 ng/mL, respectively). Based on these data, plasma yields more consistent results than serum, even after several freeze-thaw cycles, and handling of monoclonal antibodies or time of centrifugation has no significant effect on measured PAG.

scholarly journals Freeze-Thaw Splitting Strength Analysis of PAC Based on the Gray-Markov Model

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Baoyang Yu ◽  
Zongguang Sun ◽  
Lin Qi
Keyword(s):  
Markov Model ◽  
Strength Analysis ◽  
Rapid Decline ◽  
Water Stability ◽  
Freezing And Thawing ◽  
Sem Images ◽  
Freeze Thaw ◽  
Split Ratio ◽  
Repeated Freezing ◽  
Markov Method

In this study, a freeze-thaw split test was carried out to simulate the frost-heaving behavior of permeable asphalt concrete (PAC). Furthermore, the water stability problems caused by spalling and loosening were studied. Through a comparative analysis of the freeze-thaw split ratio of porosities of 19%, 21%, and 24%, the PAC porosity with excellent water stability was determined to be 19–21%. Scanning electron microscopy (SEM) images of PAC with the three porosity values after repeated freezing and thawing verified that the porosities were greater than 24% and the asphalt film peeling area was the largest, resulting in the rapid decline of the PAC freeze-thaw split ratio. The Gray-Markov model was used to predict the water stability of the mixture with a porosity of 21%. Based on the results, a Gray-Markov method for evaluating the PAC water stability in seasonally frozen areas was introduced.

scholarly journals Irrigation Scheduling Based on Wireless Sensors Output and Soil-Water Characteristic Curve in Two Soils

Sensors ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 1336 ◽  
Author(s):  
J.D. Jabro ◽  
W.B. Stevens ◽  
W.M. Iversen ◽  
B.L. Allen ◽  
U.M. Sainju
Keyword(s):  
Soil Moisture ◽  
Moisture Content ◽  
Soil Water ◽  
Root Zone ◽  
Sandy Loam ◽  
Characteristic Curve ◽  
Field Capacity ◽  
Irrigation Scheduling ◽  
Clay Loam ◽  
Soil Water Characteristic Curve

Data-driven irrigation planning can optimize crop yield and reduce adverse impacts on surface and ground water quality. We evaluated an irrigation scheduling strategy based on soil matric potentials recorded by wireless Watermark (WM) sensors installed in sandy loam and clay loam soils and soil-water characteristic curve data. Five wireless WM nodes (IRROmesh) were installed at each location, where each node consisted of three WM sensors that were installed at 15, 30, and 60 cm depths in the crop rows. Soil moisture contents, at field capacity and permanent wilting points, were determined from soil-water characteristic curves and were approximately 23% and 11% for a sandy loam, and 35% and 17% for a clay loam, respectively. The field capacity level which occurs shortly after an irrigation event was considered the upper point of soil moisture content, and the lower point was the maximum soil water depletion level at 50% of plant available water capacity in the root zone, depending on crop type, root depth, growth stage and soil type. The lower thresholds of soil moisture content to trigger an irrigation event were 17% and 26% in the sandy loam and clay loam soils, respectively. The corresponding soil water potential readings from the WM sensors to initiate irrigation events were approximately 60 kPa and 105 kPa for sandy loam, and clay loam soils, respectively. Watermark sensors can be successfully used for irrigation scheduling by simply setting two levels of moisture content using soil-water characteristic curve data. Further, the wireless system can help farmers and irrigators monitor real-time moisture content in the soil root zone of their crops and determine irrigation scheduling remotely without time consuming, manual data logging and frequent visits to the field.

scholarly journals Experimental Investigation of the Variation of Concrete Pores under the Action of Freeze-Thaw Cycles by Using X-Ray CT

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Jie Yuan ◽  
Yang Liu ◽  
Hongxia Li ◽  
Baokun Zhang
Keyword(s):  
Experimental Investigation ◽  
Image Analysis ◽  
Failure Process ◽  
Statistical Characteristics ◽  
Freezing And Thawing ◽  
X Ray ◽  
Freeze Thaw ◽  
Complete Disintegration ◽  
Repeated Freezing ◽  
Extent Of Damage

The variation of concrete pores under the action of freeze-thaw cycles was investigated experimentally by using the X-ray CT. Firstly, the statistical characteristics of pores of concrete specimens were obtained by using the X-ray image analysis. Secondly, the variation of porosity and pore volume of concrete pores were analyzed and discussed by comparing with above characteristics. Thirdly, the failure process of the concrete specimens acted by the freeze-thaw cycles was investigated by scanning the interior of concrete specimens. The results showed that the pore volumes of concrete pores whose volumes were located at the interval [0.5 mm3, 20 mm3] have no big variation in both the amounts and volume of concrete pores, while others were found to have huge change during the process of experiment. The extent of damage acted by the repeated freezing and thawing gradually ranged from surface to complete disintegration of the interior of concrete specimens after 30 cycles of freeze-thaw acting.

scholarly journals Hydrothermal Effects of Freeze-Thaw in the Taklimakan Desert

2021 ◽  
Vol 13 (3) ◽  
pp. 1292
Author(s):  
Liu Xinchun ◽  
Kang Yongde ◽  
Chen Hongna ◽  
Lu Hui
Keyword(s):  
Soil Moisture ◽  
Moisture Content ◽  
Ambient Temperature ◽  
Soil Temperature ◽  
Soil Moisture Content ◽  
Soil Freezing ◽  
Taklimakan Desert ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
The Taklimakan Desert

The Taklimakan Desert, also known as the “Sea of Death”, is the largest desert in China and also the world’s second largest remote desert. The road crossing the Taklimakan Desert is the longest desert road in the world and has been the center of the Silk Road since ancient times. Based on field observation data (November 2013 to May 2014) collected from the Tazhong and Xiaotang stations, we studied the interannual and diurnal variations of soil temperature, soil moisture content, and surface heat fluxes during different freezing and thawing periods. The annual and daily changes of soil temperature, soil moisture content, and surface energy fluxes at different freezing and thawing stages were analyzed. We illustrated the coupling relationship between water and heat in freezing-thawing soil in the Taklimakan Desert. We established a coupling model of soil water and heat during freezing and thawing. During the soil freezing period, the soil temperatures at different depths generally trended downward. The temperature difference between the Tazhong station and the Xiaotang station was 4~8.5 °C. The freezing time of soil at 20 cm depth occurred about 11 days after that at 10 cm depth. The effect of ambient temperature on soil temperature gradually weakened with the increase of soil depth. With the occurrence of the soil freezing process, the initial soil moisture contents at 5 cm, 10 cm, 20 cm, and 40 cm depths at the Xiaotang station were 6%, 10%, 29%, and 59%, respectively, and those at the Tazhong station were 5%, 3.6%, 4.4%, and 5.8%, respectively. As the ambient temperature decreased, the freezing front continued to move downward and the liquid soil water content at each depth decreased. The desert highway is closely related to the economic development and prosperity of southern Xinjiang. Therefore, it is important to maintain and inspect the safety and applicability of freeze-thaw zones and avoid casualties from vehicles and personnel.

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