Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus)

In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100µg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine alphas1-casein sequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to 27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified in the group of kids. This is the first report of a birth of a transgenic goat in Latin America.

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Embryo recovery and transfer for the production of transgenic goats from Korean native strain, Capra hircus aegagrus

Small Ruminant Research ◽

10.1016/s0921-4488(99)00139-x ◽

2000 ◽

Vol 37 (1-2) ◽

pp. 57-63 ◽

Cited By ~ 15

Author(s):

C.S Lee ◽

N.Z Fang ◽

D.B Koo ◽

Y.S Lee ◽

G.D Zheng ◽

...

Keyword(s):

Capra Hircus ◽

Native Strain ◽

Embryo Recovery ◽

Transgenic Goats

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182LAPAROSCOPIC EMBRYO TRANSFER IN KOREAN BLACK GOATS

Reproduction Fertility and Development ◽

10.1071/rdv16n1ab182 ◽

2004 ◽

Vol 16 (2) ◽

pp. 213

Author(s):

S.T. Shin ◽

S.K. Jang ◽

H.S. Yang ◽

O.K. Lee ◽

Y.H. Shim ◽

...

Keyword(s):

Embryo Transfer ◽

Efficient Method ◽

Pregnancy Rates ◽

Capra Hircus ◽

Operating Table ◽

Polyethylene Tube ◽

Pronuclear Microinjection ◽

Duration Of Surgery ◽

Transgenic Embryos

In this study, laparoscopic embryo transfer (ET) was conducted to overcome the disadvantages of laparotomic ET including invasiveness, adhesions and duration of surgery in Korean black goats (Capra hircus aegagrus). Transferred transgenic embryos were produced by DNA pronuclear microinjection of in vivo derived zygotes. Recipient goats were synchronized in estrus by using an intravaginal progesterone device CIDR® for 13 days and injection of 400IU PMSG 48hrs before CIDR® removal. Embryos were transferred on day 4 after CIDR® removal. Recipient goats were starved for 48hrs and suspended head down in an operating table at an angle of 45° to the horizontal under general anesthesia. A Veress needle (Vomed, Tuttlingen, Germany) was inserted through the abdominal wall to make a pneumoperitoneum. After obtaining sufficient pneumoperitoneum, a 5-mm laparoscope and grasping forceps (MGB, Berlin, Germany) were inserted through the 5-mm trocar sleeves. After investigation of the ovaries, uterine horns and oviducts, embryos in a polyethylene tube (SP 65, Nastume, Tokyo, Japan) were transferred into the oviduct via the infundibulum in 76 recipients. To compare the pregnancy rates, laparotomic ET was also conducted in 21 recipients. In both groups, two microinjected embryos were transferred per recipient. Pregnancy of the recipient goats was examined by ultrasound on day 30 after embryo transfer. Pregnancy rates of laparoscopic ET were significantly higher than those of laparotomic ET (46.1% v. 28.6%; P<0.05). Our results suggest that laparoscopic ET is a highly efficient method for the transfer of goat embryos.

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Successful interspecific embryo transfer between spanish ibex (Caora pyrenaica) and domestic goat (Capra hircus) using micro-osmotic pumps for FSH administration

Theriogenology ◽

10.1016/0093-691x(96)84720-0 ◽

1996 ◽

Vol 45 (1) ◽

pp. 247 ◽

Cited By ~ 3

Author(s):

A. Fernández-Arias ◽

J. Folch ◽

J.L. Alabart ◽

J.P. Ramon

Keyword(s):

Embryo Transfer ◽

Capra Hircus ◽

Domestic Goat ◽

Osmotic Pumps

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Abstract #908: Intravenous Intralipid Therapy is Not Beneficial in Having a Live Delivery in Women Aged 40-42 with a Previous History of Miscarriage or Failure to Conceive Despite Embryo Transfer Undergoing in Vitro Fertilization-Embryo Transfer

Endocrine Practice ◽

10.1016/s1530-891x(20)42788-0 ◽

2015 ◽

Vol 21 ◽

pp. 181-182

Author(s):

Jerome Check ◽

Diane Check

Keyword(s):

In Vitro Fertilization ◽

Embryo Transfer ◽

Previous History ◽

Vitro Fertilization ◽

History Of

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Experience of using time-lapse microscopy in IVF and ICSI programs

Medical alphabet ◽

10.33667/2078-5631-2020-16-47-50 ◽

2020 ◽

pp. 47-50

Author(s):

N. V. Saraeva ◽

N. V. Spiridonova ◽

M. T. Tugushev ◽

O. V. Shurygina ◽

A. I. Sinitsyna

Keyword(s):

Pregnancy Rate ◽

Embryo Transfer ◽

Selection Procedure ◽

Time Lapse ◽

Single Embryo Transfer ◽

Main Group ◽

Clinical Pregnancy ◽

Control Group ◽

Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

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Production of interferon by red deer (Cervus elaphus) conceptuses and the effects of roIFN-tau on the timing of luteolysis and the success of asynchronous embryo transfer

Reproduction ◽

10.1530/reprod/118.2.387 ◽

2000 ◽

pp. 387-395 ◽

Cited By ~ 6

Author(s):

KJ Demmers ◽

HN Jabbour ◽

DW Deakin ◽

AP Flint

Keyword(s):

Embryo Transfer ◽

Early Pregnancy ◽

Cervus Elaphus ◽

Pregnancy Loss ◽

Red Deer ◽

Interferon Tau ◽

Recombinant Interferon ◽

Uterine Flushing ◽

Calving Rate

The role of interferon in early pregnancy in red deer was investigated by (a) measuring production of interferon by the conceptus, (b) testing the anti-luteolytic effect of recombinant interferon-tau in non-pregnant hinds, and (c) treatment of hinds with interferon after asynchronous embryo transfer. Blastocysts were collected from 34 hinds by uterine flushing 14 (n = 2), 16 (n = 2), 18 (n = 8), 20 (n = 13) or 22 (n = 9) days after synchronization of oestrus with progesterone withdrawal. Interferon anti-viral activity was detectable in uterine flushings from day 16 to day 22, and increased with duration of gestation (P < 0.01) and developmental stage (P < 0.01). When interferon-tau was administered daily between day 14 and day 20 to non-pregnant hinds to mimic natural blastocyst production, luteolysis was delayed by a dose of 0.2 mg day(-1) (27.3 +/- 1.3 days after synchronization, n = 4 versus 21 +/- 0 days in control hinds, n = 3; P < 0.05). Interferon-tau was administered to hinds after asynchronous embryo transfer to determine whether it protects the conceptus against early pregnancy loss. Embryos (n = 24) collected on day 6 from naturally mated, superovulated donors (n = 15) were transferred into synchronized recipients on day 10 or day 11. Interferon-tau treatment (0.2 mg daily from day 14 to 20) increased calving rate from 0 to 64% in all recipients (0/11 versus 7/11, P < 0.005), and from 0 to 67% in day 10 recipients (0/8 versus 6/9, P < 0.01). The increased success rate of asynchronous embryo transfer after interferon-tau treatment in cervids may be of benefit where mismatched embryo-maternal signalling leads to failure in the establishment of pregnancy.

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