Seroprevalence and risk factors for bovine brucellosis in Jordan

This study was conducted to characterize the epidemiology of bovine brucellosis in the state of Pernambuco, Brazil. The state was divided into three regions, and in each region, approximately 300 properties were randomly sampled. From these selected properties, a pre-established number of animals were randomly selected and blood serum samples were obtained. A total of 3,901 animals were selected from 900 properties. For each selected property, an epidemiological questionnaire was administered to assess the type of farming, the animal husbandry practices and the sanitary practices that could be associated with the presence of brucellosis infection. The testing protocol consisted of screening the samples with a buffered acidified plate antigen test and retesting the positive samples with a complement fixation test (CF). One positive animal was enough to define an infected herd. The prevalence rates of infected herds and animals in the state were 4.5% [3.2; 6.4%] and 1.4% [0.7; 2.7%], respectively. By region, the prevalence rates of infected herds and animals, respectively, were as follows: Zona da Mata, 3.3% [1.8; 6.1%] and 1.7% [0.5; 3.0%]; Agreste, 7.4% [4.9; 10.9%] and 1.9% [0.8; 3.0%]; and Sertão, 1.3% [0.5; 3.5%] and 0.7% [0.0; 1.6%]. Flooded pastures (OR = 2.86 [1.37; 6.42]) and the presence of 13 or more females in the herd (3rd quartile) (OR = 2.65 [1.19; 5.89]) were identified as risk factors. The existence of veterinary care emerged as a protective factor against bovine brucellosis in the state of Pernambuco (OR = 0.24 [0.10; 0.58]).

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Large-scale study of herd-level risk factors for bovine brucellosis in Brazil

Acta Tropica ◽

10.1016/j.actatropica.2016.09.016 ◽

2016 ◽

Vol 164 ◽

pp. 226-232 ◽

Cited By ~ 11

Author(s):

Ana Lourdes Arrais de Alencar Mota ◽

Fernando Ferreira ◽

José Soares Ferreira Neto ◽

Ricardo Augusto Dias ◽

Marcos Amaku ◽

...

Keyword(s):

Risk Factors ◽

Large Scale ◽

Bovine Brucellosis ◽

Herd Level ◽

Large Scale Study

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Bovine brucellosis in slaughterhouses controlled by Federal and Municipal Inspection Services in the state of Maranhão, Brazil

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000832017 ◽

2019 ◽

Vol 86 ◽

Author(s):

Anna Karoline Amaral Sousa ◽

Bruno Raphael Ribeiro Guimarães ◽

Priscila Alencar Beserra ◽

Danilo Cutrim Bezerra ◽

Ferdinan de Almeida Melo ◽

...

Keyword(s):

Risk Factors ◽

Brucella Abortus ◽

Bovine Brucellosis ◽

Blood Samples ◽

Male And Female ◽

Specific Antibodies ◽

Laboratory Results ◽

Inspection Service ◽

Serum Agglutination ◽

Do So

ABSTRACT: The aims of the current study were to investigate the prevalence of bovine brucellosis, to correlate laboratory results of serum reactive samples to the serology of animals presenting serous pouches, and to identify possible risk factors for the development of the disease. In order to do so, 1,265 bovine blood samples were collected from male and female animals bred in different counties in Maranhão state, Brazil, and in other regions of the country. The animals were slaughtered in two slaughterhouses controlled by the Federal Inspection Service (FIS), and in two others controlled by the Municipal Inspection Service (MIS), of regions such as Açailândia and Imperatriz. Samples were tested for specific antibodies by using buffered acidified antigen (BAA) and 2-mercaptoethanol in combination with slow serum agglutination (2-ME + SSA). Additionally, an epidemiological questionnaire was applied to 100 owners, who provided animals to the slaughterhouses, to enable investigating the risk factors for bovine brucellosis. Fifteen serous pouches of animals presenting this lesion were also collected at slaughter time. Thirty-nine out of the analyzed samples were reacted to BAA, whereas 15 reacted to the 2-ME + SSA test; only one male was reagent and it resulted in 1.19% prevalence. One out of the 15 collected serous pouches reacted to the 2-ME + SSA test. The risk factors identified in this study were: the incidence of miscarriages, the sale of animals without previous examination, and the failure in testing the animals before introducing them in herds and before slaughter. It was possible to conclude that the animals slaughtered in the herein evaluated slaughterhouses were infected with Brucella abortus, as well as that this infection prevailed in females.

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Seroprevalence and Associated Risk Factors of Bovine Brucellosis at the Wildlife-Livestock-Human Interface in Rwanda

Microorganisms ◽

10.3390/microorganisms8101553 ◽

2020 ◽

Vol 8 (10) ◽

pp. 1553

Author(s):

Jean Bosco Ntivuguruzwa ◽

Francis Babaman Kolo ◽

Richard Simba Gashururu ◽

Lydia Umurerwa ◽

Charles Byaruhanga ◽

...

Keyword(s):

Risk Factors ◽

Potential Risk ◽

Urban Areas ◽

Small Ruminants ◽

Raw Milk ◽

Bovine Brucellosis ◽

Urban District ◽

Cross Sectional ◽

Human Interface ◽

Potential Risk Factors

Bovine brucellosis is endemic in Rwanda; however, little information is available on seroprevalence and risk factors. Therefore, a cross-sectional study was conducted among cattle farmed at the wildlife-livestock-human interface (n = 1691) in five districts and one peri-urban district (n = 216). Cattle were screened using the Rose Bengal test, then the results were confirmed by indirect enzyme-linked immunesorbent assay. Potential risk factors were determined with a questionnaire and analyzed for their association with seropositivity. In all districts, the animal and herd-level seroprevalence was 7.4% (141/1907) and 28.9% (61/212), respectively, 8.3% (141/1691) and 30.9% (61/198) at the interface, and 0.0% (0/216) in peri-urban areas. Among the potential risk factors, old age (≥5 years), cattle farmed close to wildlife, herds of cattle and small ruminants, history of abortions, and replacement animals were significantly associated with brucellosis (p < 0.05). Low awareness of zoonotic brucellosis, assisting calving without biosafety protection, drinking raw milk, and manual milking were each observed in more than 21.7% of cattle keepers whose herds were seropositive. This study confirmed brucellosis endemicity in cattle farmed close to wildlife in Rwanda, suggesting the need to focus control efforts in these areas. Educated farmers with a high awareness of zoonotic brucellosis had low bovine brucellosis seropositivity, which emphasizes the importance of education.

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