In Vitro Biodegradation of Palm Oil Mill Effluent (POME) by Bacillus subtilis, Pseudomonas aeruginosa and Aspergillus niger

2016 ◽  
Vol 7 (4) ◽  
Author(s):  
Okwute Ojonoma Loretta ◽  
Stephen E
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Palm Oil ◽  
Palm Oil Mill Effluent ◽  
Palm Oil Mill

scholarly journals Karakterisasi PHA yang dihasilkan oleh Pseudomonas aeruginosa dan Bacillus subtilis yang ditumbuhkan dalam media limbah cair pabrik kelapa sawit Characterization of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis inoculated in palm oil mill effluent (POME) media

2016 ◽  
Vol 82 (2) ◽  
Author(s):  
Irma KRESNAWATY ◽  
Agustin Sri MULYATNI ◽  
Deden Dewantara ERIS ◽  
Haryo Tejo PRAKOSO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Palm Oil ◽  
Ft Ir ◽  
Pha Production ◽  
Palm Oil Mill ◽  
Scanning Electron

AbstractThe difficulties in processing of petroleum-based plastic waste had encouraged the development of biodegradable plastics polyhydroxyalkanoate (PHA). Researchers isolated the PHA-producing microorganisms from various sources to obtain new species with high PHA production capability. In addition, the high cost of PHA production might be overcome by using carbon-rich waste, such as palm oil mill effluent (POME). This research conducted characterization of produced PHA and optimization of PHA production in POME. In previous research, three potential isolates were obtained, which are one Pseudomonas aeruginosa isolate and two Bacillus subtilis isolates. Analysis of Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed the presence of PHA accumu-lation within the bacterial cell. The results of Spectra of Fourier Transform Infra Red Spectroscopy (FT-IR) revealed differences in C-C and C-H alipathic regions of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis. Optimum production of PHA was obtained using POME at concentration of 50-25% during 6 days of incubation time in an enriched media pretreatment.Abstrak Sulitnya pengolahan limbah plastik berbasis minyak bumi mendorong pengembangan plastik biodegradable poli-hidroksialkanoat (PHA). Beberapa peneliti mengisolasi mikroorganisme penghasil  PHA dari berbagai sumber karena diharapkan akan diperoleh spesies baru dengan kemampuan produksi PHA yang tinggi. Selain itu kendala tingginya biaya produksi PHA dapat diatasi dengan peman-faatan limbah yang kaya akan  karbon, seperti limbah cair pabrik kelapa sawit (LCPKS). Pada penelitian ini dilakukan karakterisasi PHA yang dihasilkan dan optimasi produksi PHA pada LCPKS. Pada penelitian sebelumnya telah diperoleh  tiga isolat potensial, yaitu : satu isolat  Pseudo-monas aeruginosa dan dua isolat  Bacillus subtilis.  Analisis Scanning Electron Microscopy (SEM) dan Transmission Electon Microscopy (TEM) menunjukkan adanya akumulasi PHA di dalam sel bakteri. Dari hasil analisis FT-IR disimpulkan bahwa senyawa PHA yang dihasilkan Pseudo-monas aeruginosa  berbeda dengan  Bacillus subtilisyang ditandai   perbedaan   pada  spectra   gugus  C-C  dan   C-H alifatik.  Produksi optimum PHA diperoleh pada konsentrasi LCPKS 50-25%, waktu inkubasi  enam hari dan optima-lisasi pertumbuhan pada media kaya di awal untuk mening-katkan populasi mikroba.

scholarly journals Karakterisasi PHA yang dihasilkan oleh Pseudomonas aeruginosa dan Bacillus subtilis yang ditumbuhkan dalam media limbah cair pabrik kelapa sawit Characterization of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis inoculated in palm oil mill effluent (POME) media

2016 ◽  
Vol 82 (2) ◽  
Author(s):  
Irma KRESNAWATY ◽  
Agustin Sri MULYATNI ◽  
Deden Dewantara ERIS ◽  
Haryo Tejo PRAKOSO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Palm Oil ◽  
Ft Ir ◽  
Pha Production ◽  
Palm Oil Mill ◽  
Scanning Electron

AbstractThe difficulties in processing of petroleum-based plastic waste had encouraged the development of biodegradable plastics polyhydroxyalkanoate (PHA). Researchers isolated the PHA-producing microorganisms from various sources to obtain new species with high PHA production capability. In addition, the high cost of PHA production might be overcome by using carbon-rich waste, such as palm oil mill effluent (POME). This research conducted characterization of produced PHA and optimization of PHA production in POME. In previous research, three potential isolates were obtained, which are one Pseudomonas aeruginosa isolate and two Bacillus subtilis isolates. Analysis of Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed the presence of PHA accumu-lation within the bacterial cell. The results of Spectra of Fourier Transform Infra Red Spectroscopy (FT-IR) revealed differences in C-C and C-H alipathic regions of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis. Optimum production of PHA was obtained using POME at concentration of 50-25% during 6 days of incubation time in an enriched media pretreatment.Abstrak Sulitnya pengolahan limbah plastik berbasis minyak bumi mendorong pengembangan plastik biodegradable poli-hidroksialkanoat (PHA). Beberapa peneliti mengisolasi mikroorganisme penghasil  PHA dari berbagai sumber karena diharapkan akan diperoleh spesies baru dengan kemampuan produksi PHA yang tinggi. Selain itu kendala tingginya biaya produksi PHA dapat diatasi dengan peman-faatan limbah yang kaya akan  karbon, seperti limbah cair pabrik kelapa sawit (LCPKS). Pada penelitian ini dilakukan karakterisasi PHA yang dihasilkan dan optimasi produksi PHA pada LCPKS. Pada penelitian sebelumnya telah diperoleh  tiga isolat potensial, yaitu : satu isolat  Pseudo-monas aeruginosa dan dua isolat  Bacillus subtilis.  Analisis Scanning Electron Microscopy (SEM) dan Transmission Electon Microscopy (TEM) menunjukkan adanya akumulasi PHA di dalam sel bakteri. Dari hasil analisis FT-IR disimpulkan bahwa senyawa PHA yang dihasilkan Pseudo-monas aeruginosa  berbeda dengan  Bacillus subtilisyang ditandai   perbedaan   pada  spectra   gugus  C-C  dan   C-H alifatik.  Produksi optimum PHA diperoleh pada konsentrasi LCPKS 50-25%, waktu inkubasi  enam hari dan optima-lisasi pertumbuhan pada media kaya di awal untuk mening-katkan populasi mikroba.

scholarly journals Optimization of fresh palm oil mill effluent biodegradation with Aspergillus niger and Trichoderma virens

2016 ◽  
Vol 42 (1) ◽  
pp. 63-73 ◽  
Author(s):  
Noorbaizura Jalaludin ◽  
Roshanida A. Rahman ◽  
Firdausi Razali ◽  
Hind F.A Barghash ◽  
Siti Sabrina Mohd Sukri
Keyword(s):  
Aspergillus Niger ◽  
Response Surface ◽  
Palm Oil ◽  
Fungal Biomass ◽  
Inoculum Size ◽  
Palm Oil Mill Effluent ◽  
Agitation Rate ◽  
Trichoderma Virens ◽  
Optimization Strategy ◽  
Palm Oil Mill

Abstract In this work, response surface optimization strategy was employed to enhance the biodegradation process of fresh palm oil mill effluent (POME) by Aspergillus niger and Trichoderma virens. A central composite design (CCD) combined with response surface methodology (RSM) were employed to study the effects of three independent variables: inoculum size (%), agitation rate (rpm) and temperature (°C) on the biodegradation processes and production of biosolids enriched with fungal biomass protein. The results achieved using A. niger were compared to those obtained using T. virens. The optimal conditions for the biodegradation processes in terms of total suspended solids (TSS), turbidity, chemical oxygen demand (COD), specific resistance to filtration (SRF) and production of biosolids enriched with fungal biomass protein in fresh POME treated with A. niger and T. virens have been predicted by multiple response optimization and verified experimentally at 19% (v/v) inoculum size, 100 rpm, 30.2°C and 5% (v/v) inoculum size, 100 rpm, 33.3°C respectively. As disclosed by ANOVA and response surface plots, the effects of inoculum size and agitation rate on fresh POME treatment process by both fungal strains were significant.

scholarly journals Actividad antimicrobiana de cuatro plantas del nor-oriente peruano

2009 ◽  
Vol 12 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Julio R. Ruiz Q. ◽  
Mirtha Roque A.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Staphylococcus Epidermidis ◽  
Microsporum Canis ◽  
Terminalia Catappa

Se investigó la actividad antimicrobiana in vitro de los extractos metanólicos, etanólicos e hidroalcohólicos de cuatro plantas del nor-oriente peruano: Cassia reticulata (planta entera), Ilex guayusa Loes (hojas), Piper lineatum (hojas), y Terminalia catappa (hojas). Las especies fueron recolectadas en el departamento de Cajamarca, excepto Terminalia catappa (Amazonas). La actividad antimicrobiana se evaluó mediante el método de difusión en agar. Los microorganismos utilizados fueron las bacterias Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacillus subtilis y Escherichia coli; y los hongos Candida albicans, Aspergillus niger y Microsporum canis. De doce extractos investigados, ocho (67%) presentaron actividad antimicrobiana significativa frente a Staphylococcus aureus y Staphylococcus epidermidis y uno (8%) frente a Escherichia coli. De doce extractos investigados, diez (83%) presentaron actividad significativa frente a Candida albicans, y seis (50%) contra Microsporum canis. Los extractos con la mejor actividad actimicrobiana fueron los tres extractos del Piper lineatum; el extracto hidroalcohólico de Cassia reticulata y el hidroalcohólico de Terminalia catappa.

scholarly journals In VitroDegradation of Bitumen from Tar Sand by Microorganisms around the Bitumen Deposit

2020 ◽  
Vol 27 (1) ◽  
pp. 97-104
Author(s):  
U.F. Okpo ◽  
A.T. Aborisade
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Weight Loss ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Arthrobotrys Oligospora ◽  
In Vitro Degradation ◽  
Highest Weight ◽  
Percentage Weight ◽  
Cell Densities

In vitro degradation of bitumen by microorganisms isolated around bitumen deposit at Agbabu was investigated. The microorganisms were isolated from soil sample collected around bitumen deposit and bitumen itself. The ability of individual isolates to utilize bitumen as sole carbon source in mineral salt medium was investigated. The results showed a decrease in the pH of the medium with an increase in the bacteria cell densities within the period of incubation, thereby confirming activities of the isolates in the medium. Bacillus subtilis (29.15%) caused highest weight loss from the bitumen, while Pseudomonas aeruginosa (12.03%) caused the least among the bacteria isolate. Other bacteria isolated were Acinetobacter sp. and Staphylococcus aureus. All the isolates caused weight loss from the bitumen. For moulds, Arthrobotrys oligospora caused higher percentage weight loss (19.13%), than Aspergillus niger in 28 days of incubation. By the 56 days of incubation, Bacillus subtilis was responsible for highest weight loss (49.50%), while Pseudomonas aeruginosa exhibited the lowest percentage (23.17%). For the moulds, Arthrobotrys oligospora caused the higher weight loss (42.83%) while Aspergillus niger had 37.33%. Thus, this established that the microorganisms isolated from the soil around the Agbabu bitumen and the bitumen itself could degrade bitumen from tar sand, hence their potential usefulness in remediation of bitumen polluted environment when the exploitation of the resource commenced. Keywords: Bitumen; Bacteria; Mould; Hydrocarbon

Production of a bioflocculant from Aspergillus niger using palm oil mill effluent as carbon source

2014 ◽  
Vol 171 ◽  
pp. 66-70 ◽  
Author(s):  
Ahmad H. Rajab Aljuboori ◽  
Yoshimitsu Uemura ◽  
Noridah Binti Osman ◽  
Suzana Yusup
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Palm Oil ◽  
Palm Oil Mill Effluent ◽  
Palm Oil Mill
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