scholarly journals Evaluating Penetration Depth of Treatment Fluids into Dentinal Tubules Using the GentleWave System

Dentistry ◽  
2016 ◽  
Vol 06 (03) ◽  
Author(s):  
Prashanthi Vandrangi
Keyword(s):  
Penetration Depth ◽  
Dentinal Tubules

scholarly journals Debunking the Concept of Dentinal Tubule Penetration of Endodontic Sealers: Sealer Staining with Rhodamine B Fluorescent Dye Is an Inadequate Method

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3211
Author(s):  
David Donnermeyer ◽  
Sina Schmidt ◽  
Arno Rohrbach ◽  
Johannes Berlandi ◽  
Sebastian Bürklein ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Rhodamine B ◽  
Root Canal ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Ah Plus ◽  
Root Dentin ◽  
Rhodamine B Dye

The aim of this study was to investigate the suitability of rhodamine B dye staining of an epoxy resin sealer (AH Plus) and calcium-silicate-based sealers (Total Fill BC Sealer, BioRoot RCS) to represent the penetration depth of the sealers into dentinal tubules after root canal obturation. In a three-step process, (1) leaching of rhodamine B from sealers into a buffer solution, (2) passive penetration of leached rhodamine B into dentinal tubules, and (3) conformity of rhodamine B penetration assessed by confocal laser scanning microscopy (CLSM), and sealer penetration assessed by scanning electron microscopy (SEM), in root-canal-filled teeth, were evaluated. Rhodamine B dye massively leached out of Total Fill BC Sealer and BioRoot RCS into the phosphate-buffered saline (PBS). A pinkish coloration of AH Plus was found after contact with PBS. Leached rhodamine B dye passively penetrated dentinal tubules from all three sealers when placed on root dentin. No correlation was observed between sealer penetration in SEM and rhodamine B penetration in CLSM. Staining of sealers using rhodamine B is an inadequate method with which to evaluate sealer penetration depth into dentinal tubules, as it overestimates the penetration of sealers into root dentin tubules.

Comparative evaluation of sealer penetration depth into radicular dentinal tubules using confocal scanning microscope: an in vitrostudy

2020 ◽  
Vol 02 (03) ◽  
pp. 69-74
Author(s):  
Ambika Sigadam ◽  
Kalyan Satish R ◽  
Girija S Sajjan ◽  
Madhu Varma K ◽  
Sita Ram Kumar M ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Comparative Evaluation ◽  
Scanning Microscope ◽  
Dentinal Tubules ◽  
Confocal Scanning

scholarly journals Penetration Depth of Sodium Hypochlorite in Dentinal Tubules after Conventional Irrigation, Passive Ultrasonic Agitation and Nd:YAG Laser Activated Irrigation

2016 ◽  
Vol 7 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Abdollah Ghorbanzadeh ◽  
Mohsen Aminsobhani ◽  
Khosro Sohrabi ◽  
Nasim Chiniforush ◽  
Sarvenaz Ghafari ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Sodium Hypochlorite ◽  
Nd:Yag Laser ◽  
Dentinal Tubules ◽  
Ultrasonic Agitation ◽  
Conventional Irrigation

scholarly journals Penetration depth of sodium hypochlorite into dentinal tubules influenced by different agitation systems

Endodontology ◽  
2021 ◽  
Vol 33 (2) ◽  
pp. 81
Author(s):  
KeyurM Karad ◽  
SwapnilJ Kolhe ◽  
MeenalN Gulve ◽  
GayatriB Aher ◽  
PriyankaS Kolhe
Keyword(s):  
Penetration Depth ◽  
Sodium Hypochlorite ◽  
Dentinal Tubules

scholarly journals The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a Streptococcus mutans Cultivated Environment

2021 ◽  
Vol 14 (11) ◽  
pp. 1124
Author(s):  
Jung-Chang Kung ◽  
Wei-Hsun Wang ◽  
Yu-Ching Chiang ◽  
Yuan-Ting Yang-Wang ◽  
Yueh-Ching Wang ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Laser Irradiation ◽  
Penetration Depth ◽  
Streptococcus Mutans ◽  
Er:Yag Laser ◽  
Dentin Hypersensitivity ◽  
Simultaneous Application ◽  
Dentinal Tubules ◽  
X Ray ◽  
Mesoporous Bioactive Glass

The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a Streptococcus mutans cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against Streptococcus mutans was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of S. mutans, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.

The impact of irrigation protocols on epoxy sealer penetration depth in dentinal tubules. Study involving laser confocal microscopy

2021 ◽  
Author(s):  
Wojciech Wilkoński ◽  
Lidia Jamróz‐Wilkońska ◽  
Mariusz Kępczyński ◽  
Szczepan Zapotoczny ◽  
Urszula Maziarz ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Penetration Depth ◽  
Laser Confocal Microscopy ◽  
Dentinal Tubules ◽  
The Impact

scholarly journals Minimal Dentinal Tubule Penetration of Endodontic Sealers in Warm Vertical Compaction by Direct Detection via SEM Analysis

2021 ◽  
Vol 10 (19) ◽  
pp. 4440
Author(s):  
Sina Schmidt ◽  
Edgar Schäfer ◽  
Sebastian Bürklein ◽  
Arno Rohrbach ◽  
David Donnermeyer
Keyword(s):  
Penetration Depth ◽  
Direct Detection ◽  
Filling Material ◽  
Dentinal Tubule ◽  
Root Canal Filling ◽  
Dentinal Tubules ◽  
Root Canals ◽  
Ah Plus ◽  
Warm Vertical Compaction ◽  
Endodontic Sealers

Sealer staining using rhodamine B dye to investigate the penetration depth of endodontic sealers was proven unsuitable for this purpose. This study aimed to investigate the sealer penetration depth into dentinal tubules by scanning electron microscopy (SEM). Root canals of 52 human upper central incisors were instrumented using the ProTaper Gold NiTi system (Dentsply Sirona, York, PA, USA) up to size F3. After irrigation with sodium hypochlorite and citric acid combined with ultrasonic activation, the root canals were either filled using the epoxy resin sealer AH Plus (Dentsply Sirona) or the calcium silicate-based sealer Total Fill BC Sealer HiFlow (TFHF, FKG Dentaire, La Chaux-de-Fonds, Switzerland) by warm vertical compaction. Root slices of 1 mm thickness were obtained at 2 to 3, 5 to 6 and 8 to 9 mm from the apex. The root slices were investigated for sealer penetration into the dentinal tubules using SEM according to four root quadrants (buccal, mesial, oral, distal). Statistical analysis was performed by the Kruskal-Wallis test (p = 0.05) as data were not normally distributed according to the Shapiro-Wilk test. AH Plus penetrated significantly deeper into the dentinal tubules compared to TFHF at each root level (p < 0.05). Dentinal sealer penetration was deeper in the bucco-oral direction compared to the mesio-distal direction. AH Plus penetrated deeper into dentinal tubules than TFHF. Warm vertical compaction exerting high pressure on the root canal filling material is not able to press sealers deep into dentinal tubules as penetration depth values did not exceed a mean of 110 µm in SEM.

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