scholarly journals Evaluation of Antimicrobial Efficacy and Penetration Depth of Various Irrigants into the Dentinal Tubules with and without Lasers: A Stereomicroscopic Study

2019 ◽  
Vol 12 (4) ◽  
pp. 273-279 ◽  
Author(s):  
MS Saravanakumar ◽  
Gopalakrishnan Anjana ◽  
Indu Raghavan
Keyword(s):  
Penetration Depth ◽  
Antimicrobial Efficacy ◽  
Dentinal Tubules

scholarly journals Comparative Evaluation of Antimicrobial Efficacy, Depth of Penetration into Dentinal Tubules and Effect on Microhardness of Root Dentin by Sodium Hypochlorite, Neem Extract and Gau Ark as Root Canal Irrigants: An In-vitro Study

2020 ◽  
Author(s):  
Dhanshri Shashikant Khade ◽  
ND Shashikiran ◽  
Sachin Gugawad ◽  
Namrata Gaonkar ◽  
Swapnil Taur ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Sodium Hypochlorite ◽  
Normal Saline ◽  
In Vitro Study ◽  
Antimicrobial Efficacy ◽  
Depth Of Penetration ◽  
Neem Extract ◽  
Dentinal Tubules ◽  
Root Dentin

Introduction: Disinfection of root canals can be done using intracanal irrigants. Sodium hypochlorite or NaOCL is amongst the most commonly used endodontic irrigants. Due to disadvantages of NaOCl, there is a need to identify a more biocompatible irrigant. Aim: To evaluate the antibacterial efficacy, depth of penetration into dentinal tubules and effect on microhardness of root dentin when NaOCl, Neem extract, Gau ark and Normal saline used as an irrigant against Enterococcus faecalis. Materials and Methods: Prepared Neem leaf extract, 3% NaOCl, Gau ark, Normal saline, Brain Heart Infusion (BHI) broth and BHI Agar were used in the present in-vitro study conducted from August-October 2019. Antimicrobial efficacy was measured by agar well diffusion method on 10 petriplates. After access opening and biomechanical preparation, each tooth sample was irrigated with 5 mL of irrigant. Samples were decoronated and roots were split along the longitudinal axis to check penetration depth of irrigants under stereomicroscope for 40 teeth samples. After preparation of 40 teeth sample, 2 mm dentin discs prepared were embedded in acrylic resin. Microhardness was checked under Vickers Microhardness (VHN) tester after samples were irrigated. Statistical Analysis of Variance and Tukey post-hoc test were used. Results: Zones of inhibition were highest with NaOCl (7.66 mm). Irrigants penetration depth into dentinal tubules was significant with NaOCl with average value 0.35 mm. Microhardness of root dentin of saline was 145.90 VHN, followed by Neem 136.59 VHN which was then followed by Gau ark 123.05 VHN hence, Neem has lesser effect on microhardness value as compared to NaOCl and Gau ark. There was statistically significant difference among four groups regarding antimicrobial efficacy, depth of penetration into dentinal tubules and microhardness of root dentin. Conclusion: Neem extract showed significant antimicrobial property as compared to Gau ark and normal saline, good penetration depth and less effect on microhardness of root dentin. Herbal irrigant like Neem extract can be used as a root canal irrigant.

scholarly journals Debunking the Concept of Dentinal Tubule Penetration of Endodontic Sealers: Sealer Staining with Rhodamine B Fluorescent Dye Is an Inadequate Method

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3211
Author(s):  
David Donnermeyer ◽  
Sina Schmidt ◽  
Arno Rohrbach ◽  
Johannes Berlandi ◽  
Sebastian Bürklein ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Rhodamine B ◽  
Root Canal ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Ah Plus ◽  
Root Dentin ◽  
Rhodamine B Dye

The aim of this study was to investigate the suitability of rhodamine B dye staining of an epoxy resin sealer (AH Plus) and calcium-silicate-based sealers (Total Fill BC Sealer, BioRoot RCS) to represent the penetration depth of the sealers into dentinal tubules after root canal obturation. In a three-step process, (1) leaching of rhodamine B from sealers into a buffer solution, (2) passive penetration of leached rhodamine B into dentinal tubules, and (3) conformity of rhodamine B penetration assessed by confocal laser scanning microscopy (CLSM), and sealer penetration assessed by scanning electron microscopy (SEM), in root-canal-filled teeth, were evaluated. Rhodamine B dye massively leached out of Total Fill BC Sealer and BioRoot RCS into the phosphate-buffered saline (PBS). A pinkish coloration of AH Plus was found after contact with PBS. Leached rhodamine B dye passively penetrated dentinal tubules from all three sealers when placed on root dentin. No correlation was observed between sealer penetration in SEM and rhodamine B penetration in CLSM. Staining of sealers using rhodamine B is an inadequate method with which to evaluate sealer penetration depth into dentinal tubules, as it overestimates the penetration of sealers into root dentin tubules.

Comparative evaluation of sealer penetration depth into radicular dentinal tubules using confocal scanning microscope: an in vitrostudy

2020 ◽  
Vol 02 (03) ◽  
pp. 69-74
Author(s):  
Ambika Sigadam ◽  
Kalyan Satish R ◽  
Girija S Sajjan ◽  
Madhu Varma K ◽  
Sita Ram Kumar M ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Comparative Evaluation ◽  
Scanning Microscope ◽  
Dentinal Tubules ◽  
Confocal Scanning

scholarly journals Penetration Depth of Sodium Hypochlorite in Dentinal Tubules after Conventional Irrigation, Passive Ultrasonic Agitation and Nd:YAG Laser Activated Irrigation

2016 ◽  
Vol 7 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Abdollah Ghorbanzadeh ◽  
Mohsen Aminsobhani ◽  
Khosro Sohrabi ◽  
Nasim Chiniforush ◽  
Sarvenaz Ghafari ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Sodium Hypochlorite ◽  
Nd:Yag Laser ◽  
Dentinal Tubules ◽  
Ultrasonic Agitation ◽  
Conventional Irrigation

scholarly journals Penetration depth of sodium hypochlorite into dentinal tubules influenced by different agitation systems

Endodontology ◽  
2021 ◽  
Vol 33 (2) ◽  
pp. 81
Author(s):  
KeyurM Karad ◽  
SwapnilJ Kolhe ◽  
MeenalN Gulve ◽  
GayatriB Aher ◽  
PriyankaS Kolhe
Keyword(s):  
Penetration Depth ◽  
Sodium Hypochlorite ◽  
Dentinal Tubules

scholarly journals The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a Streptococcus mutans Cultivated Environment

2021 ◽  
Vol 14 (11) ◽  
pp. 1124
Author(s):  
Jung-Chang Kung ◽  
Wei-Hsun Wang ◽  
Yu-Ching Chiang ◽  
Yuan-Ting Yang-Wang ◽  
Yueh-Ching Wang ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Laser Irradiation ◽  
Penetration Depth ◽  
Streptococcus Mutans ◽  
Er:Yag Laser ◽  
Dentin Hypersensitivity ◽  
Simultaneous Application ◽  
Dentinal Tubules ◽  
X Ray ◽  
Mesoporous Bioactive Glass

The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a Streptococcus mutans cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against Streptococcus mutans was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of S. mutans, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.

Antibacterial Efficacy of Pastes Against E Faecalis in Primary Root Dentin: A Confocal Microscope Study

2015 ◽  
Vol 39 (3) ◽  
pp. 247-254 ◽  
Author(s):  
R Verma ◽  
DS Sharma ◽  
AK Pathak
Keyword(s):  
Enterococcus Faecalis ◽  
Primary Teeth ◽  
Univariate Analysis ◽  
Primary Root ◽  
Pediatric Dentistry ◽  
Antimicrobial Efficacy ◽  
Bacterial Suspension ◽  
Dead Cell ◽  
Dentinal Tubules ◽  
Significant Difference

Objectives. Management of abscessed primary teeth often present endodontic failure owing to questioned efficiency of dressings or obturating pastes to eliminate Enterococcus faecalis, a resistant bacterium, residing in depth of dentinal tubules. The present study evaluates the antimicrobial efficacy of two antibacterial and two obturating pastes in dentinal tubules of primary teeth infected with Enterococcus faecalis using viability stain and confocal laser scanning microscope (CLSM). Study design. Total 28 samples were prepared.Four groups with 6 samples each were made according to antibacterial pastes i.e. 1% or 2%Chlorhexidine (CHX) + calcium hydroxide (CH), CH + iodoform (Metapex) and Zinc Oxide Eugenol (ZOE). Dentinal tubules from the root canal side were infected with E. faecalis by centrifugation of the bacterial suspension. Two specimens from each group were subjected to 1, 7 and 15 days antibacterial pastes exposure. Viability staining followed by CLSM were used to quantitatively analyze the dead cell count directly inside dentin. Result: Univariate analysis showed that all medicaments were significantly effective (p<.05). Kruskal wallis ANOVA test did not show significant difference among four medicaments at day 1 while it was significantly different at day 7 & 15. Paired sample student’s t-test revealed significant difference in efficacy between 1& 15 days for 1%CHX+CH; between 1&15,and 7&15 days; between all days for ZOE. Ranking of antimicrobial efficacy of tested medicament was (most effective to the least): 1%CHX+CH(15)> ZOE(15)> Metapex(15)> 2%CHX+CH(15)> 2%CHX+CH(7)> 2%CHX+CH(1)> 1%CHX+CH(7)> 2%CHX+CH(15)> Metapex(1)> ZOE(1)> ZOE(7). Conclusions: All medicaments were effective against E. faecalis in dentine of primary teeth and their efficacy increased with longer contact with 1%CHX+CH being most effective at day 15. Inclusion of 1% CHX in dressings or obturating pastes might minimize the endodontic relapse and maximize the tooth retention in functional state in pediatric dentistry.

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