scholarly journals Multiplex PCR-based detection of Salmonella typhimurium and Salmonella enteritidis in Specific Pathogen Free (SPF) and Commercial Eggs

2016 ◽  
Vol 05 (02) ◽  
Author(s):  
Jakeen El Jakee ◽  
Diaa El Din Gad Khelfa
Keyword(s):  
Salmonella Typhimurium ◽  
Multiplex Pcr ◽  
Salmonella Enteritidis ◽  
Specific Pathogen Free ◽  
Specific Pathogen

In Vitro Attachment of Salmonella typhimurium to Chicken Cecal Mucus: Effect of Cations and Pretreatment with Lactobacillus spp. Isolated from the Intestinal Tracts of Chickens

1998 ◽  
Vol 61 (3) ◽  
pp. 265-271 ◽  
Author(s):  
S. E. CRAVEN ◽  
D. D. WILLIAMS
Keyword(s):  
Salmonella Typhimurium ◽  
Chelating Agents ◽  
Culture Supernatant ◽  
Divalent Cations ◽  
Supernatant Fluid ◽  
Specific Pathogen Free ◽  
Intestinal Mucus ◽  
Specific Pathogen ◽  
Lactobacillus Spp

The attachment of radiolabeled Salmonella typhimurium 3333/O cells to immobilized cecal mucus from specific-pathogen-free leghorn chickens was determined in the presence of d-mannose. The attachment of S. typhimurium was inhibited by the chelating agents EDTA and citrate and by lanthanum but was enhanced in the presence of the calcium, barium, and manganese divalent cations. Summary findings of the effect of lectins are included. Attachment of lactobacilli, previously isolated from the intestines of chickens, to mucus was also enhanced by calcium and inhibited by chelators. The pretreatment of immobilized mucus with portions of cultures of five of eight strains of lactobacilli inhibited subsequent attachment of the S. typhimurium strain. Spent culture supernatant fluid and/or washed cells from these cultures inhibited attachment, and inhibition was enhanced by preheating the cells or supernatant fluid at 80°C. Results indicate that S. typhimurium mucus attachment not involving mannosyl-dependent receptors is influenced by presence of cations. Lactobacillus spp. isolated from the intestinal tracts of chickens produce cellular and cell-free components that inhibit this form of attachment to chicken intestinal mucus.

scholarly journals Effect of Antibiotics on the Colonization of Live Attenuated Salmonella Enteritidis Vaccine in Chickens

2021 ◽  
Vol 8 ◽  
Author(s):  
Jiangang Hu ◽  
Chuanyan Che ◽  
Jiakun Zuo ◽  
Xiangpeng Niu ◽  
Zhihao Wang ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Salmonella Enteritidis ◽  
Antibiotic Use ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Specific Pathogen Free ◽  
Live Vaccines ◽  
The World ◽  
Specific Pathogen ◽  
Effective Use

Salmonellosis, caused by Salmonella Enteritidis, is a prevalent zoonosis that has serious consequences for human health and the development of the poultry sector. The Salmonella Enteritis live vaccine (Sm24/Rif12/Ssq strain) is used to prevent Salmonella Enteritidis around the world. However, in some parts of the world, poultry flocks are frequently raised under intensive conditions, with significant amounts of antimicrobials to prevent and treat disease and to promote growth. To investigate whether antibiotic use influences the colonization of orally administered Salmonella live vaccines, 240 1-day-old specific pathogen-free chicks were randomly divided into 24 groups of 10 animals for this study. The different groups were treated with different antibiotics, which included ceftiofur, amoxicillin, enrofloxacin, and lincomycin–spectinomycin. Each group was immunized 2, 3, 4, and 5 days after withdrawal, respectively. At 5 days after immunization, the blood, liver, and ceca with contents were collected for the isolation of the Salmonella live vaccine strain. The result showed that no Salmonella vaccine strain was isolated in the blood and liver of the chicks in those groups. The highest number of Salmonella vaccine strains was isolated in the cecum from chicks vaccinated 2 days after ceftiofur withdrawal, and no Salmonella vaccine strain was isolated from the cecum in chicks immunized 3 days after ceftiofur withdrawal. Among the chickens immunized 4 days after the withdrawal of amoxicillin, enrofloxacin, and lincomycin–spectinomycin, the number of Salmonella vaccine colonization in the cecum was the highest, which was higher than that of the chickens immunized at other withdrawal interval (2, 3, and 5 days) groups and was higher than that of the chickens without treatment (P < 0.05). This study provides a reference for the effective use of the Salmonella Enteritidis live vaccine and key antibiotics commonly utilized in the poultry industry.

Bacterial Isolates from the Chicken Gizzard and Ceca with In Vitro Inhibitory Activity against Salmonella typhimurium

1997 ◽  
Vol 60 (2) ◽  
pp. 120-124 ◽  
Author(s):  
DOUGLAS E. COSBY ◽  
STEPHEN E. CRAVEN ◽  
MARK A. HARRISON ◽  
NELSON A. COX
Keyword(s):  
Salmonella Typhimurium ◽  
Inhibitory Activity ◽  
Specific Pathogen Free ◽  
Bacterial Isolates ◽  
Gram Stain ◽  
Ammonium Sulfate Precipitation ◽  
Gram Negative ◽  
Specific Pathogen ◽  
Lactobacillus Spp

Bacterial isolates (197) obtained from the gizzard and ceca of 20 broiler and 40 specific-pathogen-free chickens, 21 days to 8 months of age, were evaluated for inhibitory activity against Salmonella typhimurium. One-hundred forty strains were characterized as gram negative and oxidase negative, typical of the Enterobacteriaceae. Five of the gram-negative and oxidase-negative isolates demonstrated inhibitory activity against six strains of S. typhimurium after 10- and 20-fold concentration and ammonium sulfate precipitation of the cell-free supernatant fluid from a culture grown in M9 minimal medium. Three isolates were identified as lactobacilli, 40 other strains exhibited Gram stain, oxidase, and catalase reactions typical of the Lactobacillus spp., and three known lactobacilli were included in the evaluation. Limited inhibitory activity was exhibited by these 46 isolates when tested against six S. typhimurium strains. Fourteen other strains not characterized as presumptive enterobacteria or lactic acid bacteria demonstrated little or no inhibitory activity against the six test strains.

Effects of Gamma Irradiation on the Viability and Phenotypic Characteristics of Salmonella Enteritidis Inoculated into Specific-Pathogen-Free Eggs

2011 ◽  
Vol 74 (12) ◽  
pp. 2031-2038 ◽  
Author(s):  
ELIZABETH C. P. RODRIGUES ◽  
MAURO C. L. SOUZA ◽  
SANDRO S. TOLEDO ◽  
CELSO G. BARBOSA ◽  
ELIANE M. F. REIS ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Salmonella Enteritidis ◽  
Egg Yolk ◽  
Specific Pathogen Free ◽  
Bacterial Strains ◽  
Phenotypic Characteristics ◽  
Shell Eggs ◽  
Lactose Fermentation ◽  
Specific Pathogen ◽  
60Co Radiation

The goal of this study was to determine the effects of various levels of gamma irradiation on the phenotypic characteristics of 20 strains of Salmonella Enteritidis inoculated separately into specific-pathogen-free shell eggs. Bacterial strains were inoculated into egg yolks and exposed to 60Co radiation at doses of 0.49 to 5.0 kGy. The eggs were maintained at 25°C and analyzed for the presence of Salmonella on days 1, 2, 4, and 7, and the recovered Salmonella isolates were characterized biochemically. All strains were resistant to doses of 0.49, 0.54, 0.59, 0.8, and 1 kGy; colony counts were ≥105 CFU/ml of egg yolk except for one strain, which was detected at 96 h and at 7 days after irradiation at 1 kGy, with a population reduction of 2 log CFU/ml. For the other evaluated doses, 12 strains (60.0%) were resistant at 1.5 kGy and 7 strains (35.0%) were resistant at 3.0 kGy. Among all analyzed strains, 5.0 kGy was more effective for reducing and/or eliminating the inoculated bacteria; only two (10%) strains were resistant to this level of irradiation. Salmonella colony counts were significantly reduced (P < 0.01) with increasing doses from the day 1 to 7 of observation, when microbial growth peaked. Loss of mobility, lactose fermentation, citrate utilization, and hydrogen sulfide production occurred in some strains after irradiation independent of dose and postirradiation storage time. Increases in antibiotic susceptibility also occurred: seven strains became sensitive to β-lactams, two strains became sensitive to antifolates, and one strain each became sensitive to fluoroquinolone, phenicol, nitrofurans, tetracyclines, and aminoglycosides. The results indicate that up to 5.0 kGy of radiation applied to shell eggs inoculated with Salmonella Enteritidis at 4 log CFU per egg is not sufficient for complete elimination of this pathogen from this food matrix.

Evaluation of a Multiplex PCR Assay for the Identification of Salmonella Serovars Enteritidis and Typhimurium Using Retail and Abattoir Samples

2017 ◽  
Vol 80 (2) ◽  
pp. 295-301 ◽  
Author(s):  
Dele Ogunremi ◽  
Susan Nadin-Davis ◽  
Andrée Ann Dupras ◽  
Imelda Gálvan Márquez ◽  
Katayoun Omidi ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Multiplex Pcr ◽  
Internal Control ◽  
Broiler Chickens ◽  
Salmonella Enteritidis ◽  
Bacterial Species ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Retail Outlets ◽  
Dna Fragment

ABSTRACT A multiplex PCR was developed to identify the two most common serovars of Salmonella causing foodborne illness in Canada, namely, serovars Enteritidis and Typhimurium. The PCR was designed to amplify DNA fragments from four Salmonella genes, namely, invA gene (211-bp fragment), iroB gene (309-bp fragment), Typhimurium STM 4497 (523-bp fragment), and Enteritidis SE147228 (612-bp fragment). In addition, a 1,026-bp ribosomal DNA (rDNA) fragment universally present in bacterial species was included in the assay as an internal control fragment. The detection rate of the PCR was 100% among Salmonella Enteritidis (n = 92) and Salmonella Typhimurium (n = 33) isolates. All tested Salmonella isolates (n = 194) were successfully identified based on the amplification of at least one Salmonella-specific DNA fragment. None of the four Salmonella DNA amplicons were detected in any of the non-Salmonella isolates (n = 126), indicating an exclusivity rate of 100%. When applied to crude extracts of 2,001 field isolates of Salmonella obtained during the course of a national microbiological baseline study in broiler chickens and chicken products sampled from abattoir and retail outlets, 163 isolates, or 8.1%, tested positive for Salmonella Enteritidis and another 80 isolates, or 4.0%, tested as Salmonella Typhimurium. All isolates identified by serological testing as Salmonella Enteritidis in the microbiological study were also identified by using the multiplex PCR. The new test can be used to identify or confirm pure isolates of the two serovars and is also amenable for integration into existing culture procedures for accurate detection of Salmonella colonies.

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