Acid-Pepsin Soluble Collagen from Saltwater and Freshwater Fish Scales

In this study, hydroxyapatite (HAp) was extracted from freshwater fish scales and saltwater fish scales using thermal decomposition method at various temperatures. The percentages of HAp in each species of raw fish scales were analysed using thermogravimetry analysis (TGA) whereas Fourier transform infrared (FTIR) was used to confirm the present of HA. The crystallinity of the HAp was studied using x-ray diffractometer (XRD). TGA and FTIR are found to be useful techniques to predict the composition of the HAp present in the fish scales. The results show that the composition of HAp in the fish scales was not significantly dependent on the species of the fish. The percentage of HAp in the fish scales ranged from 40 to 45 wt%. The heating temperature had an effect on the crystallinity and colour of the HAp produced. Results from FTIR and XRD confirmed that the HAp extracted from fish scales was similar to the HAp standard. However, the natural HAp produced from saltwater fish scales was more crystalline than that produced from freshwater fish scales. The HAp produced from this study is relatively cheap and more importantly they are HALAL for four billion Muslims population around the world.

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Advanced Glycation End Products of the Maillard Reaction in Aortic Pepsin-Insoluble and Pepsin-Soluble Collagen From Diabetic Rats

Diabetes ◽

10.2337/diab.45.8.1037 ◽

1996 ◽

Vol 45 (8) ◽

pp. 1037-1043 ◽

Cited By ~ 25

Author(s):

J. Meng ◽

N. Sakata ◽

S. Takebayashi ◽

T. Asano ◽

T. Futata ◽

...

Keyword(s):

Maillard Reaction ◽

Advanced Glycation End Products ◽

Diabetic Rats ◽

Advanced Glycation ◽

Soluble Collagen ◽

Glycation End Products ◽

End Products ◽

Pepsin Soluble Collagen

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Preparation and Characterisation of Collagen from Freshwater Fish Scales

Food and Nutrition Sciences ◽

10.4236/fns.2011.28112 ◽

2011 ◽

Vol 02 (08) ◽

pp. 818-823 ◽

Cited By ~ 26

Author(s):

Fengxiang Zhang ◽

Anning Wang ◽

Zhihua Li ◽

Shengwen He ◽

Lijun Shao

Keyword(s):

Freshwater Fish ◽

Fish Scales

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Extraction and Partial Characterization of Pepsin-Soluble Collagens from the Skin of Amiurus Nebulosus

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.2926 ◽

2011 ◽

Vol 236-238 ◽

pp. 2926-2934 ◽

Cited By ~ 4

Author(s):

Li Li Chen ◽

Li Zhao ◽

Hua Liu ◽

Run Feng Wu

Keyword(s):

Type I Collagen ◽

Type I ◽

Alternative Source ◽

Soluble Collagen ◽

Acid Soluble Collagen ◽

Sds Page ◽

Skin Collagen ◽

Maximal Yield ◽

Pepsin Soluble Collagen

Pepsin-soluble collagen (PSC) was successfully extracted from the skin of Amiurus nebulosus. The skin of Amiurus nebulosus was immersed in 0.3 mol/L acetic acid (1: 20, m: V) for 6 h at 37°C, while pepsin was added, at a level of 5000U/g dosage of defatted skin. The maximal yield of the collagen was 97.44%, which was higher than that of acid-soluble collagen (ASC) at 62.05%. Some properties of pepsin-soluble collagens from the skin of Amiurus nebulosus were characterized. Amino acid composition and SDS-PAGE suggested that the collagen might be classified as type I collagen. Moreover, FTIR investigations showed the existence of helical arrangements in PSC of Amiurus nebulosus skin of collagen. There is a possibility to use Amiurus nebulosus skin collagen as an alternative source of collagen for industrial purposes and subsequently it may maximize the economical value of the fish.

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