scholarly journals Effects of Different Broth Enrichment upon Phage Magnetoelastic Biosensor for Fast Detecting Low <i>Salmonella</i> Counts on Problematic Produce

2019 ◽  
Vol 09 (06) ◽  
pp. 541-555 ◽  
Author(s):  
I-Hsuan Chen ◽  
Yuzhe Liu ◽  
Songtao Du ◽  
Jiajia Hu ◽  
Fengen Wang ◽  
...  
Keyword(s):  
Broth Enrichment

scholarly journals Comparing the Yield of Staphylococcus aureus Recovery with Static versus Agitated Broth Incubation

2018 ◽  
Vol 2018 ◽  
pp. 1-3
Author(s):  
Carol E. Muenks ◽  
Patrick G. Hogan ◽  
Carey-Ann D. Burnham ◽  
Stephanie A. Fritz
Keyword(s):  
Staphylococcus Aureus ◽  
Enrichment Culture ◽  
Culture Method ◽  
Ambient Air ◽  
Built Environments ◽  
Culture Methods ◽  
Semiquantitative Assessment ◽  
Direct Plating ◽  
Static Conditions ◽  
Broth Enrichment

Given the lack of standardization of methodologies for microbial recovery from built environments, we sought to compare the yield of Staphylococcus aureus with a broth enrichment method when incubated in agitated versus static conditions. Five unique strains of S. aureus at five different concentrations were cultured to compare direct plating, agitated broth enrichment, and static broth enrichment culture methods. All samples were incubated at 35° in ambient air. The lowest concentration recovered across three replicates and five strains did not differ between culture methods (Fisher’s exact test, p=0.50); notably, recovery of S. aureus was equivalent between static and agitated broth incubation. When broth enrichment was used (both static and agitated), the burden of S. aureus growth was higher (by semiquantitative assessment of 4-quadrant streaking) compared to the direct plating culture method. Optimizing strategies for microbial recovery is essential, particularly in areas of lower biomass, given the paucity of research concerning microbial communities of built environments. The results of this study, in conjunction with other experiments investigating microbiomes of built environments, can help inform protocols for standardizing culturing methods within built environments.

scholarly journals Evaluation of Multiple Methods for Detection of Gastrointestinal Colonization of Carbapenem-Resistant Organisms from Rectal Swabs

2016 ◽  
Vol 54 (6) ◽  
pp. 1664-1667 ◽  
Author(s):  
Patricia J. Simner ◽  
Isabella Martin ◽  
Belita Opene ◽  
Pranita D. Tamma ◽  
Karen C. Carroll ◽  
...  
Keyword(s):  
High Risk ◽  
Plate Method ◽  
Carbapenem Resistant ◽  
Rectal Swabs ◽  
Direct Inoculation ◽  
Risk Patients ◽  
Gastrointestinal Colonization ◽  
Broth Enrichment ◽  
Enrichment Methods ◽  
Enrichment Step

Rectal swabs from high-risk patients were screened for carbapenem-resistant organisms (CROs) using several methods. The direct MacConkey plate method was the most sensitive for CROs (95%), while chromID CARBA and the Check-Direct CPE screen assay were the most sensitive for the detection of carbapenemase-producing organisms (CPOs) (100%; allblaKPC). All methods had a specificity of >90% for CROs, and for CPOs, the specificity ranged from 85 to 98%. Broth enrichment methods performed poorly compared to direct inoculation methods, negating the need for the broth enrichment step.

scholarly journals Development of an optimized broth enrichment culture medium for the isolation of Clostridium difficile

Anaerobe ◽  
2018 ◽  
Vol 54 ◽  
pp. 92-99 ◽  
Author(s):  
Mairéad C. Connor ◽  
John W. McGrath ◽  
Geoff McMullan ◽  
Nikki Marks ◽  
Derek J. Fairley
Keyword(s):  
Clostridium Difficile ◽  
Culture Medium ◽  
Enrichment Culture ◽  
Broth Enrichment

Transfer of Salmonella montevideo onto the Interior Surfaces of Tomatoes by Cutting†

1997 ◽  
Vol 60 (7) ◽  
pp. 858-862 ◽  
Author(s):  
CHIA-MIN LIN ◽  
CHENG-I WEI
Keyword(s):  
Recovery Rate ◽  
Enrichment Method ◽  
Direct Plating ◽  
Inoculum Dose ◽  
Scar Region ◽  
Plating Method ◽  
Safety Operation ◽  
The Relationship ◽  
Cut Surface ◽  
Broth Enrichment

Salmonella contamination of precut watermelon, tomatoes, and cantaloupes was responsible for several outbreaks of salmonellosis. To better understand the relationship between bacterial doses and their transfer onto cut surfaces by using a knife, rifampicin-resistant Salmonella montevideo at 7, 70, 700, or 7,000 CFU in Butterfield's buffer (BPB) or tryptic soy broth (TSB) was added to the stem scars of tomatoes. Tomatoes were cut from the stem scar to blossom end using a sterilized knife. After stem scars were aseptically removed, cut surfaces were placed on tryptic soy agar-rifampicin (TSA-RIF) plates or processed by a broth enrichment method to determine if S. montevideo had been transferred to the cut surface. S. montevideo was recovered in a dose-related fashion using both methods. A higher recovery rate was obtained with bacterial inocula in TSB than in BPB, and also with broth enrichment rather than the direct plating method. The distribution of the transferred S. montevideo on the cut surface of contaminated and noncontaminated tomatoes with a knife was related to the inoculum dose added to the stem scars. S. montevideo colonies were found to cluster at the stem scar region with the lower inoculum dose. However, when a higher inoculum dose was used, the colonies spread from the stem scar region to the center and bottom of cut tomatoes, or they were transferred to another uninoculated tomato by the contaminated knife. Therefore, the safety operation criteria recommended by FDA to wash fruits before cutting, to use clean and sanitized utensils and surfaces when preparing cut fruits, and to store the cut fruits below 7°C should be followed in preparing tomato slices to minimize salmonellosis outbreaks caused by this product.

Isolation of Salmonella Typhimurium from Outbreak-Associated Cake Mix

2007 ◽  
Vol 70 (4) ◽  
pp. 997-1001 ◽  
Author(s):  
GUODONG ZHANG ◽  
LI MA ◽  
NEHAL PATEL ◽  
BALA SWAMINATHAN ◽  
STEPHANIE WEDEL ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Gel Electrophoresis ◽  
Ice Cream ◽  
Pulsed Field Gel Electrophoresis ◽  
Outbreak Investigation ◽  
Cake Batter ◽  
Pulsed Field ◽  
Enrichment Cultures ◽  
Broth Enrichment ◽  
Better Than

During May and June of 2005, 26 persons in several states were infected by a single strain (isolates indistinguishable by pulsed-field gel electrophoresis) of Salmonella enterica serotype Typhimurium after eating cake batter ice cream. The cake mix used to prepare the cake batter in the ice cream was implicated by epidemiologic investigation as the source of Salmonella contamination. Initial tests did not detect Salmonella in cake mix collected during the outbreak investigation. The objective of this study was to evaluate different procedures to isolate Salmonella from the implicated cake mix, cake, and ice cream. All outbreak-associated food samples (14 samples) were collected during the outbreak investigation by health departments of several of the states involved. Different combinations of Salmonella isolation procedures, including sample size, preenrichment broth, enrichment broth, enrichment temperature, and isolation medium, were used. Salmonella Typhimurium was isolated from two cake mix samples; the food isolates were indistinguishable from the outbreak pattern by pulsed-field gel electrophoresis subtyping. Universal preenrichment broth was substantially better than was lactose broth for preenrichment, and tetrathionate broth was better than was Rappaport-Vassiliadis broth for isolating Salmonella from the two positive cake mix samples. Although more typical Salmonella colonies were observed on plates from enrichment cultures grown at 35°C, more confirmed Salmonella isolates were obtained from plates of enrichment cultures grown at 42°C. Brilliant green agar, xylose lysine tergitol 4 agar, xylose lysine desoxycholate agar, Hektoen enteric agar, and bismuth sulfite agar plates were equally effective in isolating Salmonella from cake mix. The best combination of preenrichment-enrichment conditions for isolating the outbreak strain of Salmonella was preenrichment of cake mix samples in universal preenrichment broth at 35oC for 24 h, followed by enrichment in tetrathionate broth at 42°C for 24 h.

Incubation of Egg Contents Pools at an Elevated Temperature (42°C) Does Not Improve the Rapid Detection of Salmonella Enteritidis Phage Type 14b

2004 ◽  
Vol 67 (8) ◽  
pp. 1751-1754
Author(s):  
RICHARD K. GAST ◽  
PETER S. HOLT
Keyword(s):  
Elevated Temperature ◽  
High Frequency ◽  
Rapid Detection ◽  
Salmonella Enteritidis ◽  
Phage Type ◽  
Lateral Flow ◽  
Rapid Assays ◽  
Efficient Detection ◽  
Working Day ◽  
Broth Enrichment

Detecting internal Salmonella Enteritidis (SE) contamination in eggs is essential for protecting public health. Pooling together ≥10 eggs for sampling allows many eggs to be screened for contamination, but such pools must be incubated (usually at 25 to 37°C) to permit small numbers of SE to multiply before further testing. The present study determined whether incubating egg contents pools at an elevated temperature (42°C) could increase the rate of multiplication of a phage type 14b strain of SE sufficiently to support the detection of contamination by a rapid lateral flow immunodiffusion method within a single day. Pools of 10 eggs were contaminated with approximately 10 CFU of SE, supplemented with concentrated broth enrichment medium, and incubated at either 37 or 42°C. Incubation of contaminated egg pools at 42°C resulted in significantly higher SE levels after 6, 8, 10, and 12 h. However, incubation at 42°C could only generate a mean log SE concentration of4.21 CFU/ml within a single working day (8 h), inadequate to support efficient detection by most rapid assays. Detection of SE contamination in egg pools by a rapid lateral flow immunodiffusion test was not achieved at a high frequency until 12 h of incubation at 42°C.

scholarly journals Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry

2002 ◽  
Vol 34 (1) ◽  
pp. 37-41 ◽  
Author(s):  
A. Eyigor ◽  
K.T. Carli ◽  
C.B. Unal
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Broth Enrichment ◽  
Enrichment Step

scholarly journals Modified culture technique for Corynebacterium diphtheriae isolation from desiccated swabs

1978 ◽  
Vol 7 (2) ◽  
pp. 137-138
Author(s):  
R R Facklam ◽  
D N Lawrence ◽  
F O Sottnek
Keyword(s):  
Silica Gel ◽  
Enhanced Recovery ◽  
Skin Lesions ◽  
Corynebacterium Diphtheriae ◽  
Culture Technique ◽  
Field Collection ◽  
Broth Enrichment ◽  
Culture Procedure

Corynebacterium diphtheriae was isolated from pyoderma and ulcerative skin lesions with a modified delayed culture procedure as late as 9 weeks after field collection of silica gel-desiccated swabs. Biotypes gravis and mitis were identified. Most isolates were nontoxigenic. Todd-Hewitt broth enrichment enhanced recovery of C. diphtheriae by 70%.

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