HPTLC Phytochemical Screening and Hydrophilic Antioxidant Activities of &lt;i&gt;Apium graveolens&lt;/i&gt; L., &lt;i&gt;Cleome gynandra&lt;/i&gt; L., and &lt;i&gt;Hibiscus sabdariffa&lt;/i&gt; L. Used for Diabetes Management

Background: Mangrove plant extracts are used in folkloric medicine as aphrodisiac, sedative, antioxidant, antimicrobial and antimalarial. Screening for antimicrobial, phytochemical and antioxidant activities of fourteen mangroves plant species (Aegiceras corniculatum, Acanthus ilicifolius, Avicennia alba, Avicennia marina, Avicennia officinalis, Bruguiera cylindrica, Bruguiera gymnorhiza, Ceriops tagel, Excoecaria agallocha, Kandelia candel, Rhizophora apiculata, Rhizophora mucronata, Sonneratia alba, and Sonneratia caseolaris) in various solvents are reported in this work. Methods: The antimicrobial screening was carried out using agar well diffusion method. In this study, nine pathogenic strains were used, including three Gram-positive and six Gram-negative bacteria. Phytochemical screening, total flavonoids, total phenolic and antioxidant activity was tested by DPPH radical scavenging assay. Four phenolic compounds (gallic acid, vanillin, tannic acid and quercetin) were quantified by LC-MS/MS in selected mangrove species. Results: Antimicrobial screening showed Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa were most susceptible to ethyl acetate extract as compared to ethanol and methanol extract. Ethyl acetate extract of Avicennia marina and Bruguiera gymnorhiza showed strong antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa. Phytochemical analysis revealed the presence of saponins, phenolics, flavonoids, alkaloids, tannins, and terpenoids, which was found to be variable as per the solvent used for extraction. In addition, total phenolics and total flavonoids content with different solvents were found in the range of 11.08 to 196.76 mg GAE/g and 12.92 to 110.3 mg QE/g of extract respectively. Moreover, antioxidant capacities expressed in terms of IC50 (mg/mL) showed that methanol extract exhibited higher antioxidant capacity followed by ethanol extract. LC-MS/MS analysis showed gallic acid and tannic acid are present in higher concentration in Aegiceras corniculatum and Sonneratia caseolaris than other species. The vanillin and quercetin were found in the least concentration. Conclusion: Mangroves species are rich source of antioxidant, phenolics and antimicrobial compounds.

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FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.90 ◽

2018 ◽

Vol 10 (1) ◽

pp. 407

Author(s):

Dewi Kumala Putri ◽

Berna Elya ◽

Nuraini Puspitasari

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Hexane Extract ◽

Phytochemical Screening ◽

Active Fraction ◽

Leaf Extracts ◽

Antioxidant Power ◽

Dpph Method ◽

Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

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Phytochemical Screening, Antioxidant Activities and In Vitro Anticancer Potential of Egyptian Capsicum Spp.

Biochemistry & Pharmacology Open Access ◽

10.4172/2167-0501.1000205 ◽

2015 ◽

Vol 05 (02) ◽

Cited By ~ 1

Author(s):

Shaimaa GA ◽

Mahmoud MS

Keyword(s):

Antioxidant Activities ◽

Phytochemical Screening ◽

Capsicum Spp

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Comparative Studies of the Phytochemical and Antioxidant activities of the leaves, stems, roots, and seeds extracts of Mitracarpus scaber Zucc (Rubiaceae)

Mediterranean Journal of Chemistry ◽

10.13171/mjc10702007241441cwk ◽

2020 ◽

Vol 10 (7) ◽

pp. 668

Author(s):

Wacothon Karime Coulibaly

Keyword(s):

Ascorbic Acid ◽

Phenolic Compounds ◽

Dry Matter ◽

Antioxidant Activities ◽

Phytochemical Screening ◽

Average Value ◽

Mitracarpus Scaber ◽

Layer Chromatography ◽

Gallic Acid Equivalent ◽

Ethyl Acetate Extracts

Extracts of the leaves, stem, roots, and seeds of Mitracarpus scaber plants have been thoroughly investigated in an attempt to determine their phytochemical and antioxidant activities. Phytochemical screening carried out by thin-layer chromatography (TLC) revealed the presence of several secondary metabolites in all the selective extracts of the plant. The levels of phenolic compounds showed that the leaves of M. Scaber are the richest in polyphenols with an average value of 488.291 ± 1.205 μg GAE/g (microgram of gallic acid equivalent per gram of the dry matter of the powdered plant). The total flavonoid assay revealed a high content in the leaves (1.624 ± 0.032 %). Also, studies of the antioxidant activities by DPPH● (2,2-diphenyl-1'-picrylhydrazyl) method showed a significant effect compared to vitamin C (96.62 %). The percentages of inhibition are respectively 89.82 % and 82.3 % for ethyl acetate extracts of the leaves and seeds. Furthermore, the inhibitory percentages of n-butanol in the leaves and seeds are 90.57 % and 83.67 %, respectively. Calculated IC50 showed that the n-butanol fraction of the seeds exhibited the highest activity with an IC50 of 0.293 mg/mL less than ascorbic acid with an IC50 of 0.387 mg/mL.

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Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

International Research Journal of Pure and Applied Chemistry ◽

10.9734/irjpac/2020/v21i430160 ◽

2020 ◽

pp. 1-5

Author(s):

M. Suleman Stephen ◽

E. A. Adelakun ◽

J. H. Kanus ◽

Meshack M. Gideon

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Acetone Extract ◽

Apium Graveolens ◽

Isolation And Identification ◽

Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

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