Acclimating leaf celery plant (Apium graveolens) via bottom wet culture for increasing its adaptability to tropical riparian wetland ecosystem

Lakitan B, Kartika, Susilawati, Wijaya A. 2021. Acclimating leaf celery plant (Apium graveolens) via bottom wet culture to increase its adaptability to the tropical riparian wetland ecosystem. Biodiversitas 22: 320-328. Bottom-wet culture was set up for acclimating leaf celery plant prior to cultivation at shallow water table conditions. The aim of this research was to evaluate adaptability of leaf celery plants to riparian wetland ecosystem. Leaf celery was selected as potential candidate since natural habitat of its wild relatives is marshlands. Shading at 0%, 20%, and 60% was applied to reduced tropical sunlight intensity. Results of this study indicated that soil moisture was significantly increased in plants exposed to 60% shading, but leaf SPAD value was not significantly affected. Leaf celery is a perennial vegetable that can be frequently harvested. Weekly harvesting was rewarded with optimum yield and good quality leaves, i.e. high SPAD value (45.73 to 51.89). Delaying harvest to 3 weeks increased total yield but 52.12% of the harvested leaves were non-marketable. Mother plant of leaf celery produced suckers, but number of suckers only moderately correlated with yield (R2 = 0.56). Plants exposed to 60% shading produced significantly less suckers (9.00) than those exposed to full sunlight (12.46) and 20% shading (12.88) Use of zero intercept linear regression model, with length of leaf midrib (LLM) x leaf wingspan (LWS) as predictor, resulted in a geometrically based and accurate leaf area estimation model (LA = 0.3431(LLM x LWS); R2 = 0.87) for compound leaves of leaf celery plant. In conclusion, the most crucial factor in optimizing quantity and quality of yield was weekly harvesting focusing on marketable-size leaves.

Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

PENGARUH APLIKASI PUPUK NPK TERHADAP PERTUMBUHAN DAN HASIL TANAMAN SELEDRI (Apium graveolens L.) PADA MEDIA GAMBUT

Celery (Apium graveolens L.) is a vegetable plant that has long been known in Indonesia. Celery plants have many benefits. Celery plants come from Europe, especially in the Mediterranean region around the Mediterranean Sea. This plant spreads to the Plains of China, India, Central Asia, Ethiopia, South and Central Mexico and the United States the influence of NPK fertilizer on the growth and yield of celery plants on peat media. This research was conducted on Kenanga Street, Teluk Panji IV, Kampung Rakyat Subdistrict, Labuhan Batu Selatan Regency, at an altitude of ± 23 m above sea level. This activity will be carried out from February 2019 to May 2019. The design used is a randomized single factor group design that is with NPK fertilizer dosage: Treatment P0: (control), P1: (5 g / plant) P2: (10 g / plant ). The parameters observed were plant height (cm), number of leaves (strands), number of tillers, harvest weight (g). From the results of data analysis it was found that the effect of NPK Phonska fertilizer (15; 15; 15) can increase the growth of celery plant. The highest plant height (11.93 cm) Number of leaves (6.5 strands) Number of tillers (8.66) Weight of harvest (17.5 g). NPK Phonska fertilizer (15; 15; 15) is recommended with a dose of P2: (10 g / plant). Keywords : Celery (Apium graveolens L.), NPK, Peat Soil

INDUKSI KALUS LIMA KULTIVAR SELEDRI (Apium graveolens L.) DENGAN SUKROSA DAN BERBAGAI KONSENTRASI MALTOSA

Penelitian ini bertujuan untuk mengetahui respon berbagai kultivar seledri terhadap induk sikalus dengan menggunakan sukrosa dan maltosa. Percobaan dilaksanakan dengan menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri atas dua faktor dan diulang tiga kali. Faktor pertama adalah kultivar seledri terdiri atas lima taraf yaitu Aroma, Bamby, Samantha, Sunda,Tall Utah. Faktor kedua adalah konsentrasi karbohidrat yang terdiri atas lima taraf yaitu sukrosa 20 g/L, maltosa 20 g/L), maltosa 30 g/L, maltosa 40 g/L, maltosa 60 g/L. Variabel yang diamati meliputi waktu awal kalus terbentuk, diameter kalus, warna kalus, tekstur kalus, kalus embriogenik, dan jumlah tunas pada tahap regenerasi. Hasil penelitian menunjukkan konsentrasi sukrosa 20 g/L merupakan konsentrasi terbaik terhadap kecepatan muncul kalus pada kultivar Bamby. Sukrosa 20 g/L memberikan pengaruh paling baik terhadap ukuran kalus pada kultivar Aroma. Maltosa 30 g/L mampu menginduksi kalus dari semua kultivar seledri yang digunakan. Kulvivar Samantha responsif terhadap pembentukan kalus di semua konsentrasi Maltosa yang digunakan. Kata kunci: seledri, sukrosa, maltosa, kalus Keywords: Celery, Plant Breeding, Carbohydrate, Maltose, and Callus

PENGARUH APLIKASI PUPUK NPK TERHADAP PERTUMBUHAN DAN HASIL TANAMAN SELEDRI (Apium graveolens L.) PADA MEDIA GAMBUT

Celery (Apium graveolens L.) is a vegetable plant that has long been known in Indonesia. Celery plants have many benefits. Celery plants come from Europe, especially in the Mediterranean region around the Mediterranean Sea. This plant spreads to the Plains of China, India, Central Asia, Ethiopia, South and Central Mexico and the United States the influence of NPK fertilizer on the growth and yield of celery plants on peat media. This research was conducted on Kenanga Street, Teluk Panji IV, Kampung Rakyat Subdistrict, Labuhan Batu Selatan Regency, at an altitude of ± 23 m above sea level. This activity will be carried out from February 2019 to May 2019. The design used is a randomized single factor group design that is with NPK fertilizer dosage: Treatment P0: (control), P1: (5 g / plant) P2: (10 g / plant ). The parameters observed were plant height (cm), number of leaves (strands), number of tillers, harvest weight (g). From the results of data analysis it was found that the effect of NPK Phonska fertilizer (15; 15; 15) can increase the growth of celery plant. The highest plant height (11.93 cm) Number of leaves (6.5 strands) Number of tillers (8.66) Weight of harvest (17.5 g). NPK Phonska fertilizer (15; 15; 15) is recommended with a dose of P2: (10 g / plant). Keywords : Celery (Apium graveolens L.), NPK, Peat Soil

Identifikasi Spesies Nematoda Meloidogyne sp pada Tanaman Tomat (Solanum lycopersicum L) dan Seledri (Apium graveolens L) di Samarinda

Nematodes are harmful organisms for tomato and celery plants. The aim of this research is to know Meloidogynespecies that attack tomato and celery plants. The locations are in Lempake and in Sambutan, Samarinda city, EastKalimantan. Identification of nematode species is based on morphometric and perennial parten characteristics. Nematodeextract was performed by root stenning method and nematode surgery. The result showed that there was one species(Meloidogyne javanica) of nematode at root of tomato and celery plant in Lempake, while in Sambutan showed that therewere two species of Meloidogyne, they are Meloidogyne javanica and Meloidogyne incognita. Based on this research showedthat nematodes Meloidogyne are the most enermoust nematodes on tomato and celery plants in Samarinda.

Effect of immature yellow melon seeds in inhibition of growth of cucumber and celery plant

Cucumis sativus is a vegetable crop belonging to the Cucurbitaceae family and originates from temperate, subtropical sub-humid areas. This study was aimed to evaluate the effect of water extract of immature melon seeds in inhibition of seed germination of cucumber and celery plant. This study was conducted in the laboratory of the Department of Science, Faculty of Basic Education, Diyala University, Iraq. The seeds of these vegetables were selected and diagnosed in the herbaceous plant in the Department of Life Sciences. During the washing of immature seeds that were isolated from other melon residues. The results showed that the concentration of (0.1, 0.3, 0.7) and compared to the comparison models had the same effect in germination in seeds for cucumber. For celery plant only (0.7) gave the highest percentage of inhibition of celery seed plant and reached 20% Which was 80%. As for the length of the root only, the concentration of (0.7) gave the lowest rate for the cucumber and celery which amounted to 2.132 and 0.866 respectively, compared to the comparison treatment amounted to 5.932 and 1.399 respectively, in addition to other transactions. In addition, the concentration (0.7) gave the lowest length of the cucumber and celery time of 8.799 and 2.566 respectively, compared with the comparison treatment given to both plants 13.232 and 3.066 respectively, in addition to other treatments. As for the soft and dry weight of the cucumber plant, the concentration was given (0.7) the highest rate of soft weight at 0.2920, while the concentration (0.1, 0.3) gave 0.2057 and 0.0599 respectively, while dry weight gave the concentration (0.7).

Association of ‘Candidatus Liberibacter solanacearum’ with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection

A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ‘Candidatus Liberibacter solanacearum’ and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect ‘Ca. L. solanacearum’. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ‘Ca. Liberibacter’ were observed using electron microscopy in celery plant tissues. A fifth haplotype of ‘Ca. L. solanacearum’, named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.

Diurnal Pattern of Carbohydrates in Celery Leaves of Various Ages

Diurnal fluctuations in soluble carbohydrates and starch were monitored in young (expanding), mature (first fully expanded), and old (nearing senescence) celery (Apium graveolens L.) leaves. In all tissues, mannitol and sucrose were the carbohydrates present in the highest concentrations. In old and young leaflets and their petioles, there was little change in levels of mannitol and sucrose in 26 hours. In mature leaflets, sucrose accumulated in the light and decreased in the dark; mannitol increased slightly in late afternoon. Starch concentration, although quite low, showed definite diurnal fluctuations in mature leaflets, but only small changes in young and old leaflets. Both sucrose and mannitol were present in mature petiole phloem tissues. Mannitol concentrations were high in the adjacent storage parenchyma tissue, but sucrose was almost undetectable. These data support earlier findings that sucrose is produced, translocated, and metabolized throughout the celery plant. Mannitol is also translocated, but also serves as a major storage carbohydrate in leaf tissues, especially petiole parenchyma. Starch serves as a minor short-term storage compound in leaflets.