scholarly journals Comparative Studies of the Phytochemical and Antioxidant activities of the leaves, stems, roots, and seeds extracts of Mitracarpus scaber Zucc (Rubiaceae)

2020 ◽  
Vol 10 (7) ◽  
pp. 668
Author(s):  
Wacothon Karime Coulibaly
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Dry Matter ◽  
Antioxidant Activities ◽  
Phytochemical Screening ◽  
Average Value ◽  
Mitracarpus Scaber ◽  
Layer Chromatography ◽  
Gallic Acid Equivalent ◽  
Ethyl Acetate Extracts

Extracts of the leaves, stem, roots, and seeds of <em>Mitracarpus scaber</em> plants have been thoroughly investigated in an attempt to determine their phytochemical and antioxidant activities.<em> </em>Phytochemical screening carried out by thin-layer chromatography (TLC) revealed the presence of several secondary metabolites in all the selective extracts of the plant. The levels of phenolic compounds showed that the leaves of <em>M. Scaber</em> are the richest in polyphenols with an average value of 488.291 ± 1.205 μg GAE/g (microgram of gallic acid equivalent per gram of the dry matter of the powdered plant). The total flavonoid assay revealed a high content in the leaves (1.624 ± 0.032 %). Also, studies of the antioxidant activities by DPPH<sup>● </sup>(2,2-diphenyl-1'-picrylhydrazyl) method showed a significant effect compared to vitamin C (96.62 %). The percentages of inhibition are respectively 89.82 % and 82.3 % for ethyl acetate extracts of the leaves and seeds. Furthermore, the inhibitory percentages of n-butanol in the leaves and seeds are 90.57 % and 83.67 %, respectively. Calculated IC<sub>50</sub> showed that the n-butanol fraction of the seeds exhibited the highest activity with an IC<sub>50</sub> of 0.293 mg/mL less than ascorbic acid with an IC<sub>50</sub> of 0.387 mg/mL.

Phytochemical Study and Antioxidant Activities on Extracts of the Leaves and Roots of Costus afer Ker Gawl. (Zingiberaceae)

2020 ◽  
Vol 36 (05) ◽  
pp. 804-811
Author(s):  
Coulibaly Wacothon Karime ◽  
Adiko N’dri Marcelline ◽  
Benie Anoubilé ◽  
Konan Yao Mokey Jean-Baptiste ◽  
Bea Gouanda Thibaut ◽  
...  
Keyword(s):  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Average Value ◽  
Dpph Method ◽  
Phytochemical Constituents ◽  
Leaves And Roots ◽  
Layer Chromatography ◽  
Costus Afer

The extracts of leaves and roots of Costus afer plant have been fully investigated in an attempt to determine their phytochemical constituents and antioxidant activities. Phytochemical screening carried out using thin layer chromatography (TLC) revealed the presence of several secondary metabolites in all the selected extracts of the plant. In addition, our results showed that the leaves of C. afer are the richest in polyphenols with an average value of 3416.25 μg EAG/g MS. The total flavonoid assay revealed a highest content in the leaves (8.02 %). Furthermore, studies of the antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) method showed a significant effect compared to the reference vitamin C. Ethyl acetate extract of the leaves show a higher percentage of inhibition (83 %), followed the roots (69 %).

scholarly journals Antioxidant Compounds, Mineral Content and Antioxidant Activity of Several Tomato Cultivars Grown in Southwestern Romania

2013 ◽  
Vol 41 (1) ◽  
pp. 136 ◽  
Author(s):  
Violeta NOUR ◽  
Ion TRANDAFIR ◽  
Mira E. IONICA
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Trace Elements ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Fruit Shape ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Tomato Cultivars

Ten indeterminate tomato cultivars differing in fruit shape and typology, grown simultaneously in a greenhouse from Oltenia (Southwestern Romania) and harvested at red ripe stage, were investigated for the contents in ascorbic acid, lycopene, β-carotene, total phenolics, minerals (K, Na, Ca and Mg) and trace elements (Fe, Cu, Mn, Cr, Zn and B). In addition, their antioxidant activities were determined. Significant differences were found among tomato cultivars in all studied antioxidant compounds, as well as in the antioxidant activity. Ascorbic acid ranged from 91.9 to 329.7 mg kg-1 fw, lycopene ranged from 19.7 to 49.0 mg kg-1 fw, while total phenolic compounds varied between 300.2 and 557.8 mg kg-1 fw. Antioxidant activity ranged from 0.81 mmol Trolox kg-1 fw to 1.74 mmol Trolox kg-1 fw and it was significantly correlated to total phenolics content (r = 0.91; p< 0.05). The cocktail type cultivar ‘Tiger’ and the cherry type cultivar ‘Belle’ proved to be the most powerful in antioxidant activity and phenolic compounds while the rectangular plum shaped cultivar ‘Porto’ recorded the highest average lycopene content. A valuable cultivar proved to be the brownish red ‘Sacher’ which registered among the highest contents of phenolics, lycopene and ascorbic acid. Mineral and trace elements contents were also significantly affected by cultivars. Values recorded for K, Ca and Mg ranged from 2139.6 to 3056.9 mg kg-1, 137.7 to 325.8 mg kg-1 and 27.3 to 168.7 mg kg-1 respectively.

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

scholarly journals Pharmacognostical, Physicochemical and Preliminary Phytochemical Standardization of Aerial Parts of Onosma bracteatum Wall

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Patel V. G. ◽  
Patel K. G. ◽  
Patel K. V. ◽  
Gandhi T. R.
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Microscopical Examination ◽  
Phytochemical Screening ◽  
Aerial Parts ◽  
Inorganic Acids ◽  
Physicochemical Evaluation ◽  
Thin Walled ◽  
Layer Chromatography

Objective: The present study was undertaken to develop the standardization parameters of powdered aerial parts of Onosma bracteatum Wall, family Boraginaceae. Methods: Different parameters such as pharmacognostical, physicochemical, preliminary phytochemical evaluation along with thin layer chromatography for identification of phytoconstituents were studied. Results: On microscopical examination of the aerial parts it showed the presence of oval to polygonal thin walled straight epidermal cells; spiral vessels, a few fibres elongated with blunt tips, long warty, tubercle based unicellular hairs and paracytic stomata. On physicochemical evaluation it was found to contain more amount of polar constituents as the ethanol extractive value was found to be more. Total ash value and acid insoluble ash indicated the presence of inorganic acids and silicaceous matter respectively. Foaming index and swelling index were indicative of saponins and mucilaginous matter present in the aerial parts. On preliminary phytochemical screening and thin layer chromatographic studies it revealed the presence of saponins, flavonoids, phenolic compounds and mucilage. Conclusion: The present work carried out can serve as a purpose for identification, authentication and standardization of the crude drug.

scholarly journals HPTLC and FTIR Fingerprinting of Olive Leaves Extracts and ATR-FTIR Characterisation of Major Flavonoids and Polyphenolics

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6892
Author(s):  
Snezana Agatonovic-Kustrin ◽  
Vladimir Gegechkori ◽  
Dementyev Sergey Petrovich ◽  
Kobakhidze Tamara Ilinichna ◽  
David William Morton
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Deep Eutectic Solvent ◽  
Ethanol Extract ◽  
Flavonoid Content ◽  
Olive Leaves ◽  
Polyphenolic Content ◽  
Natural Deep Eutectic Solvent ◽  
Layer Chromatography ◽  
Ethyl Acetate Extracts

The aim of this study was to analyse the effect of spontaneous microbial maceration on the release and extraction of the flavonoids and phenolics from olive leaves. Bioprofiling based on thin-layer chromatography effect-directed detection followed by ATR-FTIR spectroscopy proved to be a reliable and convenient method for simultaneous comparison of the extracts. Results show that fermentation significantly enhances the extraction of phenolic compounds and flavonoids. The polyphenolic content was increased from 6.7 µg GAE (gallic acid equivalents) to 25.5 µg GAE, antioxidants from 10.3 µg GAE to 25.3 µg GAE, and flavonoid content from 42 µg RE (rutin equivalents) to 238 µg RE per 20 µL of extract. Increased antioxidant activity of fermented ethyl acetate extracts was attributed to the higher concentration of extracted flavonoids and phenolic terpenoids, while increased antioxidant activity in fermented ethanol extract was due to increased extraction of flavonoids as extraction of phenolic compounds was not improved. Lactic acid that is released during fermentation and glycine present in the olive leaves form a natural deep eutectic solvent (NADES) with significantly increased solubility for flavonoids.

Estimation of Phenolic acid and Flavonoids in leaves of Amaranthus viridis and Amaranthus spinosus

2021 ◽  
pp. 3889-3895
Author(s):  
Swarnakumari S ◽  
Sasikala M ◽  
Mohan S ◽  
Divyanand Maharaj U ◽  
Kavipriya G
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
Methanolic Extract ◽  
Solvent System ◽  
Who Guidelines ◽  
Phytochemical Screening ◽  
Amaranthus Spinosus ◽  
Layer Chromatography

Objective: To explore physiochemical and phytochemical standardization of leaves of Amaranthus viridis and Amaranthus spinosus. Method: As per WHO guidelines, physical standardization parameters with various solvents system were evaluated. The preliminary phytochemical screenings were adopted for disclosing the existence of phytochemicals in the discrete extracts. Thin layer chromatography and HPTLC were employed for methanolic extract of leaves of Amaranthus viridis and Amaranthus spinosus. Result: Preliminary phytochemical screening with various extracts reveals phytoconstituents. HPTLC fingerprint were executed for leaves of Amaranthus viridis and Amaranthus spinosus using selected solvent system. HPTLC analysis implies the presence of phenolic compounds and flavonoids in both the plant Amaranthus viridis and Amaranthus spinosus. Conclusion: Quercetin and Gallic acid was raise to be more in Amaranthus viridis compared to Amaranthus spinosus, however rutin was reported only in Amaranthus spinosus. The outcome of the research leads for isolation, purification and utilization in herbal industries.

scholarly journals Comparison of Phenolic Compounds and Antioxidant Activities of Raw and Cooked Turkish Artichoke Cultivars

2021 ◽  
Vol 5 ◽  
Author(s):  
Seda Kayahan ◽  
Didem Saloglu
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Acid Content ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Microwave Cooking ◽  
Gallic Acid Equivalent

The objective of this work was to determine the total phenolic compounds and antioxidants in raw and cooked Sakiz and Bayrampasa variety artichokes in parts such as inner bracts, stems, receptacles, and outer bracts. The artichokes were cooked by boiling, microwaving, and baking methods, and total phenolic compounds and antioxidants of cooked artichokes were evaluated. While TPC (total phenolic content), DPPH (2,2-diphenyl-1-picryl-hydrazine), and CUPRAC values for the leaves of raw Bayrampasa artichoke were found to be 686 mg gallic acid equivalent (GAE)/100 g, 478 mg TE/100 g, and 4,875 mg TE/100 g, respectively, TPC, DPPH, and CUPRAC values for stems of Sakiz artichoke were determined to be 1,579 mg GAE/100 g, 1,259 mg TE/100 g, and 3,575 mg TE/100 g. A significant increase in the content of TPC, DPPH, and CUPRAC values was observed for all cooking applications of both artichokes. DPPH and CUPRAC values increased by 11 and 43 times and 17 and 6.7 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively. Chlorogenic acid, cynarine, and cynaroside content of both artichokes had an increment after all cooking applications. Chlorogenic acid content was improved 29 and 58 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively.

scholarly journals Phytochemical Screening and Thin Layer Chromatography Profiling of Various Extracts of Achyranthes aspera and Cissus quadrangularis

2021 ◽  
Vol 10 (4) ◽  
pp. 225-229
Author(s):  
V. Suresh Kumar ◽  
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Solvent System ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Achyranthes Aspera ◽  
Cissus Quadrangularis ◽  
Layer Chromatography

Achyranthes aspera and Cissus quadrangularis are important medicinal plants possessing wide biological activity. The natural products obtained from plants provides an unique opportunity for the development of new drugs but due to their complex nature there is a need to isolate and purify the bioactive compounds from plant extracts by separation techniques. Our earlier findings revealed that methanol extracts of A. aspera and C. quadrangularis and aqueous extract of C. quadrangularis exhibited antibacterial activity. This prompted to take up further research to reveal these plants’ potential valuable phytochemicals and therefore an attempt has been made to separate the phytochemical constituents of methanol and aqueous extracts of A. aspera and C. quadrangularis by thin layer chromatography. Phytochemical screening of both plant extracts revealed more concentrated phytochemicals in methanol extracts than in aqueous extracts. The TLC profiling showed that methanol extract of A. aspera and C. quadrangularis are rich in flavonoids and phenolic compounds. Flavonoids were separated with n-butanol:ethyl acetate:water (1:2:3) solvent system at Rf value of 0.66 and was confirmed by post-development derivatization with 3% boric acid and 10% oxalic acid spray. After derivatization, the flavonoid spot could be further visualized under UV light at 254nm. Phenolic compounds were separated with methanol:water (2:1) solvent system at Rf value of 0.88 and confirmed with 10% ferric chloride spray. From the present study, suitable mobile phase for separation of flavonoids and phenolic compound fraction from methanol and aqueous extracts of both plants and TLC profiling have been developed

scholarly journals NUTRITIONAL COMPONENTS IN SELECT FLORIDA TROPICAL FRUITS

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 504E-504
Author(s):  
Kanjana Mahattanatawee ◽  
Elizabeth Baldwin ◽  
Kevin Goodner ◽  
John Manthey ◽  
Gary Luzio
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Gallic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Tropical Fruits ◽  
Total Dietary Fiber ◽  
Soluble Phenolics ◽  
Gallic Acid Equivalent

Fourteen tropical fruits from southern Florida [red guava, white guava, carambola, red pitaya (red dragon), white pitaya (white dragon), mamey, sapodilla, lychee, longan, green mango, ripe mango, green papaya and ripe papaya] were evaluated for antioxidant activity, ascorbic acid (vitamin C), total fiber and pectin. ORAC (oxygen radical absorbance capacity) and DPPH (1,1-diphenyl-2-picrylhydrazyl, radical scavenging activity) assays were used to determine antioxidant activity. The total soluble phenolics (TSP), ORAC, and DPPH ranged from 205.4 to 2316.7 μg gallic acid equivalent/g puree, 0.03 to 16.7 μmole Trolox equivalent/g puree and 2.1 to 620.2 μg gallic acid equivalent/g puree, respectively. Total ascorbic acid (TAA), total dietary fiber (TDF) and pectin ranged from 13.6 to 159.6 mg/100 g, 0.88 to 7.25 g/100 g and 0.2 to 1.04 g/100 g, respectively. The antioxidant activities, TSP, TAA, TDF and pectin appeared to be influenced by cultivar (papaya, guava and dragon fruit) and ripening stage (papaya and/or mango). Data demonstrate the potential benefits of several of these fruits for human health.

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