scholarly journals Effect of Water Stress Induced by Polyethylene Glycol 6000 on Somatic Embryogenesis in Cocoa (<i>Theobroma cacao</i> L.)

2019 ◽  
Vol 10 (09) ◽  
pp. 1240-1254
Author(s):  
Manlé Tokpapon Eliane ◽  
Kouassi Kan Modeste ◽  
Soumahoro Brahima André ◽  
Koffi Kouablan Edmond ◽  
Koné Mongomaké
Keyword(s):  
Somatic Embryogenesis ◽  
Polyethylene Glycol ◽  
Water Stress ◽  
Theobroma Cacao ◽  
Effect Of Water ◽  
Polyethylene Glycol 6000

scholarly journals Water stress in germination, growth and development of coffee cultivars

2018 ◽  
Vol 40 (1) ◽  
pp. 82-89 ◽  
Author(s):  
Julieta Andréa Silva de Almeida ◽  
Mary Túlia Vargas Lobato Guedes de Azevedo ◽  
Marcus Vinicius Salomon ◽  
Priscila Fratin Medina
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Seed Germination ◽  
Growth And Development ◽  
Coffea Arabica ◽  
Primary Root ◽  
Control Treatment ◽  
Effect Of Water ◽  
Reduced Height ◽  
Polyethylene Glycol 6000

Abstract: The aim of this study was to evaluate the effect of water stress on the germination of Coffea arabica seeds and the growth and development of plants from these seeds. Seeds of the cultivars Bourbon Vermelho, Mundo Novo, and BA-10 were placed to germinate in the dark at 30 ºC in the presence and absence of 100 g/L of polyethylene glycol 6000. The water stress applied delayed the beginning of seed germination by seven days in relation to seeds of the control, which had achieved responses of up to 50%, but there were no differences between the treatments at the end of the experiment. Furthermore, the length of the primary root in seeds germinated under water stress was less than in the control treatment, especially for the cultivar Mundo Novo. Plants of the cultivars Bourbon Vermelho and Mundo Novo developed from seeds previously germinated under water stress exhibited reduced height and fewer leaf pairs than those from the control, whereas ‘BA-10’ responded in a similar way in both treatments. Thus, the cultivars Bourbon Vermelho and Mundo Novo were more sensitive to the water stress applied in the germination phase than ‘BA-10’.

Effects of water potential on germination of lettuce, sunflower, and citrus seeds

1969 ◽  
Vol 47 (11) ◽  
pp. 1761-1764 ◽  
Author(s):  
Merrill R. Kaufmann
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Water Potential ◽  
Cellulose Acetate Membrane ◽  
Lettuce Seeds ◽  
Water Potentials ◽  
Citrus Seeds ◽  
Germinating Seeds ◽  
Polyethylene Glycol 6000 ◽  
Selection Of

The effect of nearly constant water potential on germination of citrus, sunflower, and lettuce seeds was studied. Water potential equilibration was achieved by placing soil above a cellulose acetate membrane which was in contact with a solution of polyethylene glycol-6000. Selection of solute potentials in the solution resulted in controlled water potentials in the soil over a range of 0 to −14.9 bars for citrus and 0 to −8.0 bars for sunflower and lettuce. The water stress experienced by germinating seeds in this system is largely the result of a matric effect rather than a solute effect.Citrus seeds germinated at water potentials as low as −4.7 bars and lettuce at −4.1 bars, but sunflower germinated at −8.0 bars. Sunflower germinated as rapidly at −4.1 bars as lettuce at −2.3 bars, both reaching 50% germination at about 8 days. Citrus germinated much more slowly, requiring 26 days at 0 bars.

scholarly journals Effects of Water Stress on Germination of Yarrow Populations (Achillea Spp.) From Different Bioclimatic Zones in Iran

2014 ◽  
Vol 68 (1) ◽  
pp. 39-54 ◽  
Author(s):  
Parvin Salehi Shanjani ◽  
Masoumeh Izadpanah ◽  
Mohamad Reza Mohamadpour
Keyword(s):  
Polyethylene Glycol ◽  
Natural Selection ◽  
Water Stress ◽  
Water Potential ◽  
Wild Populations ◽  
Genetic Potential ◽  
Intraspecific Variations ◽  
Germination Ability ◽  
Genetic Pool ◽  
Polyethylene Glycol 6000

ABSTRACT The effects of water potential on germination were studied in 18 wild populations of three yarrow species (Achillea tenuifolia, A. vermicularis and A. filipendulina) from different bioclimatic zones in Iran. Water potential between 0 and -0.6 MPa were obtained using polyethylene glycol 6000 (PEG-6000) solutions. The study of water stress on germination showed that, regardless of the species and the populations, the lowering of the water potential reduced the capacity for germination and early seeding growth. These results indicated a strong genetic potential for drought tolerance during germination within each species. These differences in germination ability of wild populations of each species might be attributed to intraspecific variations resulting from the effects of natural selection and genetic pool background.

scholarly journals Effect of water stress induced by polyethylene glycol 6000 on the somatic embryos conversion into whole plantlets in cocoa (Theobroma cacao L.)

2021 ◽  
Vol 15 (1) ◽  
pp. 12-25
Author(s):  
Tokpapon Eliane Manlé ◽  
Kan Modeste Kouassi ◽  
Brahima André Soumahoro ◽  
Tchoa Koné ◽  
Kouablan Edmond Koffi ◽  
...  
Keyword(s):  
Climate Change ◽  
Polyethylene Glycol ◽  
Water Stress ◽  
Somatic Embryos ◽  
Stem Length ◽  
Improvement Program ◽  
Polyethylene Glycol 6000 ◽  
Regenerated Plantlets ◽  
Theobroma Cacao L

Rainfall scarcity due to climate change is a major constraint that limits cocoa productivity in Côte d'Ivoire. This work aims to regenerate cocoa plants tolerant to water stress using in vitro methods. Staminode and petal explants of the genotypes C1, C9, C14, C15, C16, C18 and C20 were used to produce somatic embryos through two methods. Firstly, somatic embryos were induced under stressfull conditions on media containing different concentrations of polyethylene glycol (PEG) 6000 (0; 25; 50; 75; 100 and 125 g/l) and secondly; under non-stressed conditions. Somatic embryos were placed on a conversion medium in the same stress condition. The number of regenerants decreased with the increase in the concentration of PEG with all genotypes. Only genotypes C1 and C15 regenerated plantlets under water stress conditions. The sensitive genotypes C9, C14, C16, C18 and C20 have not developed plantlets on media containing PEG. The plantlets produced under water deficit conditions exhibited a reduction in stem length and leaves number and an increase in length or offset of the high number of roots. The survival rate of regenerants during acclimatization was higher on the sandsubstrate. The selected genotypes could be used in an improvement program of cocoa production.Keywords: Climate change; plant regeneration; genotype; tolerance; drought; in vitro

scholarly journals Analisis Pengaruh Pelapisan Benih dengan Bahan Desikan dan Lama Penyimpanan Terhadap Kualitas Benih Kakao (Theobroma Cacao L.) di Pusat Penelitian Kopi dan Kakao Indonesia, Jember

Agroteknika ◽  
2018 ◽  
Vol 1 (2) ◽  
pp. 99-110
Author(s):  
Siti Suleho Batubara ◽  
John Nefri ◽  
Nofrianil
Keyword(s):  
Polyethylene Glycol ◽  
Theobroma Cacao ◽  
Polyethylene Glycol 6000 ◽  
Theobroma Cacao L

Peran benih dari perbanyakan generatif sangat besar. Benih berkualitas biasanya disediakan oleh perkebunan besar yang jauh dari perkebunan petani kecil sehingga pengiriman benih membutuhkan waktu lama. Hal ini dapat menyebabkan penurunan kualitas biji kakao. upaya untuk menjaga kualitas benih dengan perlakuan pektin desiccant dan Polyethylene glycol 6000. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian bahan pengering, lama penyimpanan, dan interaksi keduanya dalam menjaga kualitas biji kakao. Penelitian ini dilakukan di Pusat Penelitian Kopi dan Kakao Indonesia, Jember. Penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) Faktorial yang diulang dua kali. Faktor pertama adalah desikan dan faktor kedua adalah lama penyimpanan. Hasil penelitian menunjukkan bahwa bahan pengeringan memiliki pengaruh yang signifikan pada galat 1% terhadap kadar air, tingkat perkecambahan, dan indeks vigor biji kakao, dan pada tingkat 5% terhadap benih berjamur selama penyimpanan, tidak signifikan dalam Berat biji setelah disimpan. Lama penyimpanan secara signifikan pada galat 1% mempengaruhi kadar air, tingkat perkecambahan biji, dan terbukti dalam perdagangan 5% dari indeks vigor benih kakao, tidak signifikan dalam berat benih setelah simpan, dan benih berkecambah selama penyimpanan. Pengaruh kedua faktor memiliki pengaruh yang signifikan pada galat 1% terhadap kadar air, benih berjamur selama penyimpanan, perkecambahan, dan indeks vigor benih, dan tidak ada pengaruh yang signifikan terhadap berat benih setelah penyimpanan dan benih berkecambah dalam penyimpanan.

Accumulation of polyethylene glycol 6000 and its effects on water content and carbohydrate level in water-stressed tomato plants

1988 ◽  
Vol 66 (5) ◽  
pp. 970-973 ◽  
Author(s):  
E. Jacomini ◽  
A. Bertani ◽  
S. Mapelli
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Soluble Sugar ◽  
Physiological Effects ◽  
Tomato Plants ◽  
Peg 6000 ◽  
Plant Parts ◽  
Tomato Roots ◽  
Peg Treatment ◽  
Polyethylene Glycol 6000

Young tomato plants were grown in 10 or 25% polyethylene glycol (PEG) 6000 solutions to impose water stress. Plant organs were analyzed to ascertain whether the various plant parts accumulated PEG and to determine the physiological effects. PEG 6000 was taken up by tomato roots and translocated to the shoots. PEG accumulation was greater in roots and in the first leaves and negligible in apical leaves. In all leaves there was an increase in soluble-sugar concentration during the PEG treatment. However, PEG-accumulating leaves showed a delay in dehydration in comparison with leaves where PEG was not accumulated. To explain this behaviour we hypothesize that PEG was accumulated in extracellular spaces and that, as a consequence, water was drawn out from the cells, inducing cellular but not tissue dehydration.

scholarly journals Water-stress Screening in Blackgram [Vigna mungo (L.) Hepper] Genotypes using Polyethylene Glycol 6000 at Seedling Growth Stage

2020 ◽  
Author(s):  
V. A. Mohanlal ◽  
K. Saravanan ◽  
T. Sabesan
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Seedling Growth ◽  
Dry Weight ◽  
Vigna Mungo ◽  
Tolerance Index ◽  
Fresh Weight ◽  
Drought Tolerant ◽  
Polyethylene Glycol 6000 ◽  
Pot Culture

Background: Blackgram is one of the important pulse crops belongs to the Papilionaceae family. Seed germination and seedling growth are a very important step in the survival of a plant. Water stress is one of the important factors affects seedling growth. Methods: An experiment was conducted to study the effect of water stress on seedling growth in twenty-one blackgram [Vigna mungo (L.) Hepper] genotypes at the pot culture yard of Annamalai University during March 2018. The water stress was created by using Polyethylene Glycol (6000 MW) in different concentrations (0%, 10% and 20%). Different phenotypic characters were recorded such as shoot length, root length, shoot fresh weight, root fresh weight, shoot dry weight and root dry weight at two different levels of treatment and control. The experiment was laid in a completely randomized design with two replications. Result: The combined analysis of variance revealed significant differences (p less than 0.001) among genotypes, treatments and genotypes × treatments interaction. The highest stress tolerance index (STI) was recorded by the genotype ADT-5. Based on clustering and ranking, the genotypes ADT-5, KGB-28, RU-16-9, VBG-10010 and VBG-12062 were classified as drought-tolerant. Overall, pot culture screening of 21 blackgram genotypes for drought tolerance at seedling stage using various concentrations of PEG 6000 resulted in the identification of drought-tolerant genotypes, where ADT-5 showed the highest tolerance followed by KGB-28 and RU-16-9.

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