Natural coagulants from a crude water extract of common bean seed showed very
good efficiency of turbidity removal from water of ~89 % under optimal
coagulation conditions, which were determined using response surface
methodology (RSM). However, they also increased the content of organic
matter in treated model water by ~66 %, which is the main drawback of usage
of natural coagulants, in general. Thus, ultrafiltration was applied for
processing of the crude water extract in order to separate biomolecules,
which exhibit the coagulation activity. Four fractions obtained by
ultrafiltration were applied in coagulation tests under the same conditions
as the crude extract, and the 4th fraction (molecules with molecular weights
>30 kDa) with the predominant content of proteins with molecular weights 50
- 60 kDa, achieved almost as high efficiency of turbidity removal (75 %) as
the crude extract. At the same time, the content of organic matter in
treated water increased just for 16 % in comparison to the blank (model
water processed in the same way but without coagulant). After optimization
of process parameters by RSM for usage of the 4th fraction, the coagulation
activity increased further to 80 %.
Extraction and Solubilization Methods Suitable for Proteomic Analysis of Common Bean Seed of Ivory Coast
