Gingival-derived mesenchymal stem cells: An endless resource for regenerative dentistry

Despite gingival derived mesenchymal stem cells (GDMSCs) are unique and have numerous advantages for regenerative dentistry, the hypoxia preconditioning of GDMSCs may be essential prior to the transplantation. The aim of this study is to examine whether CoCl2 can enhance HIF-1α in GDMSCs in vitro. GDMSCs of Rabbit (Oryctolagus cuniculus) were obtained from laboratory stock. The sample for this study was randomly selected and divided into 4 groups; GDMSC normoxia (24 hours and 48 hours) groups and GDMSC hypoxia (24 hours and 48 hours) groups respectively (N=20/n=5). To stimulate the hypoxia condition, the final concentration of 100μM Cobalt (II) Chloride (CoCl2) was used in this study. The examination of Hypoxia Inducible Factor-1α (HIF-1α) stimulated with CoCl2 in the GDMSCs utilized immunocytochemistry methods with Fluorescein isothiocyanate antibody labelling. The Tukey Honest Significant Different (HSD) test was conducted to compare the significant difference in HIF-1α expression between groups (p<0.01). The greatest expression of HIF-1α is found in GDMSCs treated with CoCl2 for 24 hours. There was significant difference in HIF-1α expression between GMSCs normoxia (24 hours and 48 hours) and GMSCs hypoxia of 24 hours groups but not 48 hours group (p<0.01). CoCl2 can enhance significantly HIF-1α expression of GDMSCs for 24 hours in vitro.

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Regenerative Dentistry and Stem Cells: A Multilineage Differentiation as a Safe and Useful Alternative Way of Harvesting and Selection Adipose Derived Mesenchymal Stem Cells

Current Drug Targets ◽

10.2174/1389450119666180816122230 ◽

2018 ◽

Vol 19 (16) ◽

pp. 1991-1997 ◽

Cited By ~ 1

Author(s):

Dorina Lauritano ◽

Palmieri Annalisa ◽

Candotto Valentina ◽

Carinci Francesco

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Multilineage Differentiation ◽

Regenerative Dentistry

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Management of Endodontic and Periodontal Lesions: the Role of Regenerative Dentistry and Biomaterials

Dentistry Journal ◽

10.3390/dj8020032 ◽

2020 ◽

Vol 8 (2) ◽

pp. 32

Author(s):

Marco Tatullo ◽

Francesco Riccitiello ◽

Sandro Rengo ◽

Benedetta Marrelli ◽

Rosa Valletta ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Surgical Approach ◽

Interdisciplinary Approach ◽

Pivotal Role ◽

Bioactive Substances ◽

Cell Lineages ◽

Regenerative Dentistry ◽

Strategic Role

Regenerative dentistry represents a novel interdisciplinary approach involving biomaterials, several molecules and mesenchymal stem cells (MSCs), preferably derived from oral tissues. The pivotal role of MSCs depends on the fact that they can differentiate into different cell lineages and have the strategic role to release bioactive substances that stimulate the renewal and regeneration of damaged tissues. The role of regenerative dentistry is promising in all the branches of dentistry: the most intriguing application is related to the management of endodontic and periodontal defects, overcoming the surgical approach and the implantology as a consequence of a poorly efficient therapeutic plan.

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The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry

Japanese Dental Science Review ◽

10.1016/j.jdsr.2016.09.001 ◽

2017 ◽

Vol 53 (2) ◽

pp. 25-33 ◽

Cited By ~ 10

Author(s):

Kunimichi Niibe ◽

Maolin Zhang ◽

Kosuke Nakazawa ◽

Satoru Morikawa ◽

Taneaki Nakagawa ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Neural Crest ◽

Neural Crest Cell ◽

Regenerative Dentistry ◽

Cell Source ◽

A Cell ◽

Cell Phenotypes ◽

Crest Cell

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Inflammatory Cytokines and Biodegradable Scaffolds in Dental Mesenchymal Stem Cells Priming

Current Stem Cell Research & Therapy ◽

10.2174/1574888x14666190103170109 ◽

2019 ◽

Vol 14 (4) ◽

pp. 320-326 ◽

Cited By ~ 4

Author(s):

Tatjana Kanjevac ◽

Collin Gustafson ◽

Ana Ivanovska ◽

Francesca Ravanetti ◽

Antonio Cacchioli ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Inflammatory Cytokines ◽

Therapeutic Potential ◽

Multipotent Stem Cells ◽

Inflammatory Microenvironment ◽

New Findings ◽

Regenerative Dentistry ◽

Biomaterial Scaffolds ◽

Regenerate Tissue

Mesenchymal stem cells (MSCs) are multipotent stem cells with wide-ranging clinical applications due to their ability to regenerate tissue from mesenchymal origin and their capability of suppressing immune responses, thus reducing the likelihood of graft versus host disease after transplantation. MSCs can be isolated from a variety of sources including bone marrow, adipose tissue, umbilical cord blood, and immature teeth. Dental stem cells (DSCs) possess progenitor and immunomodulatory abilities as the other MSC types and because they can be easily isolated, are considered as attractive therapeutic agents in regenerative dentistry. Recently, it has been shown that DSCs seeded onto newly developed synthetic biomaterial scaffolds have retained their potential for proliferation and at the same time have enhanced capabilities for differentiation and immunosuppression. The scaffolds are becoming more efficient at MSC priming as researchers learn how short peptide sequences alter the adhesive and proliferative capabilities of the scaffolds by stimulating or inhibiting classical osteogenic pathways. New findings on how to modulate the inflammatory microenvironment, which can prime DSCs for differentiation, combined with the use of next generation scaffolds may significantly improve their therapeutic potential. In this review, we summarize current findings regarding DSCs as a potential regenerative therapy, including stem cell priming with inflammatory cytokines, types of scaffolds currently being explored and the modulation of scaffolds to regulate immune response and promote growth.

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Application of collagen and mesenchymal stem cells in regenerative dentistry

Current Stem Cell Research & Therapy ◽

10.2174/1574888x17666211220100521 ◽

2021 ◽

Vol 17 ◽

Author(s):

Mohammad Samiei ◽

Mahdieh Alipour ◽

Khadijeh Khezri ◽

Yalda Rahbar Saadat ◽

Haleh Forouhandeh ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cellular Response ◽

Matrix Elasticity ◽

Temporomandibular Joints ◽

Regenerative Dentistry ◽

Different Types ◽

Calcified Tissues ◽

Modify Collagen ◽

Host Tissues

: Collagen is an important macromolecule of extracellular matrix (ECM) in bones, teeth, and temporomandibular joints. Mesenchymal stem cells (MSCs) interact with the components of the ECM such as collagen, proteoglycans, glycosaminoglycans (GAGs), and several proteins on behalf of variable matrix elasticity and bioactive cues. Synthetic collagen-based biomaterials could be effective scaffolds for regenerative dentistry applications due to mimicking of host tissues’ ECM. These biomaterials are biocompatible, biodegradable, readily available, and non-toxic to cells whose capability promotes cellular response and wound healing in the craniofacial region. Collagen could incorporate other biomolecules to induce mineralization in calcified tissues such as bone and tooth. Moreover, the addition of these molecules or other polymers to collagen-based biomaterials could enhance mechanical properties, which is important in load-bearing areas such as the mandible. A literature review was performed via reliable internet database (mainly PubMed) based on MeSH keywords. This review first describes the properties of collagen as a key protein in the structure of hard tissues. Then, it introduces different types of collagens, the correlation between collagen and MSCs, and the methods used to modify collagen in regenerative dentistry including recent progression on the regeneration of periodontium, dentin-pulp complex, and temporomandibular joint by applying collagen. Besides, the prospects and challenges of collagen-based biomaterials in the craniofacial region pointes out.

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МЕЗЕНХІМАЛЬНІ СТОВБУРОВІ КЛІТИНИ ОДОНТОГЕННОГО ПОХОДЖЕННЯ: ПЕРСПЕКТИВИ ТА МОЖЛИВОСТІ РЕГЕНЕРАТИВНОЇ МЕДИЦИНИ

Scientific and practical journal "Stomatological Bulletin" ◽

10.35220/2078-8916-2021-41-3.6 ◽

2021 ◽

Vol 116 (3) ◽

pp. 33-40

Author(s):

О.І. Годованець ◽

К.Л. Гальчук ◽

Т.І. Муринюк ◽

Е.О. Саука

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Regenerative Medicine ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Web Of Science ◽

Regenerative Dentistry

Мета: Провести аналіз літературних джерел у напрямку науково-теоретичних та клінічних аспек- тів щодо можливостей використання мезенхімаль- них стовбурових клітин, отриманих з різних джерел щелепно-лицевої ділянки. Матеріали і методи: Під час дослідження викорис- тано бібліосемантичний метод та структурно-логіч- ний аналіз. Для пошуку сучасної наукової літератури були використані електронні бази даних PubMed, MEDLINE, Scopus, Web of Science та EMBASE за клю- човими словами «regenerative medicine», «regenerative dentistry», «stem cells», «dental mesenchymal stem cells», «stem cell therapy», «tissue engineering». Висновки: На основі проведеного аналізу літера- тури прослідковується неабиякий інтерес науковців до стовбурових клітин одонтогенного походження та їх використання у регенеративній практиці не лише стоматологічного спрямування, але і для ліку- вання соматичних хвороб різного генезу. Це пов’язано із неінвазивним та більш простим методом забору матеріалу, порівняно із кістковим мозком людини чи ембріональними тканинами. Стовбурові клітини різ- няться за походженням, диференційною активністю та джерелом їх отримання, а також мають ваго- мий потенціал до диференціації за напрямком різних клітинних ліній залежно від впливу факторів росту та живильного середовища. При одержанні нових чистих культур вдається встановити їх походження шляхом ідентифікації експресії маркерів, характерних для стовбурових клітин. Тим не менш, незважаючи на високі очікування від подальшого розвитку реге- неративної терапії, науковцям необхідно детальніше вивчити можливості використання цих клітин на етапах клінічного випробування, дослідити імуноло- гічну поведінку стовбурових клітин одонтогенного походження в тому чи іншому середовищі.

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