Differentiation of Toxoplasma Gondii from Closely Related Coccidia by Riboprint Analysis and a Surface Antigen Gene Polymerase Chain Reaction

1993 ◽  
Vol 48 (3) ◽  
pp. 447-456 ◽  
Author(s):  
Paul J. Brindley ◽  
J. P. Dubey ◽  
Eric Y. Denkers ◽  
Ricardo T. Gazzinelli ◽  
Claudio Silveira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Surface Antigen ◽  
Chain Reaction ◽  
Antigen Gene ◽  
Gene Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues

2004 ◽  
Vol 99 (2) ◽  
pp. 185-188 ◽  
Author(s):  
Márcia Andreia Barge Loução Terra ◽  
Alexandre Ribeiro Bello ◽  
Otilio Machado Bastos ◽  
Regina Reis Amendoeira ◽  
Janice Mary Chicarino de Oliveira Coelho ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Molecular diagnosis of the curly lettuce parasitic contamination from hydroponic cultivation from supermarkets

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Fernanda Pinto-Ferreira ◽  
Jonatan Batista Reis ◽  
Aline Ticiani Pereira Paschoal ◽  
Letícia Santos Balbino ◽  
Amanda Bertão-Santos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Dna Extraction ◽  
Chain Reaction ◽  
Cryptosporidium Spp ◽  
Hydroponic Cultivation ◽  
Polymerase Chain ◽  
Fluctuation Method ◽  
The City ◽  
Commercial Kit

Abstract The consumption of vegetables has increased in recent years due to the search for a healthier diet that is rich in fiber and has fewer calories. To assess the parasitic contamination of lettuce sold in markets, a survey of parasites was carried out from a supermarket chain in the city of Londrina, Paraná. A total of thirty samples of lettuce were purchased in the ten markets visited, three in each, of which ten were conventionally cultivated, ten were hydroponically cultivated, and ten were organically cultivated. All samples were analyzed using the sedimentation methods of Hoffman, Pons and Janer and the fluctuation method of Faust and colleagues and Willis with adaptations. In addition, the samples were subjected to DNA extraction by a commercial kit and polymerase chain reaction to detect Toxoplasma gondii, Cryptosporidium spp. and Giardia spp., which are protozoa that cause food and waterborne parasitic outbreaks. All samples were negative for sedimentation and flotation techniques. One of the hydroponically cultivated samples was positive for T. gondii. The results demonstrate the risk of curly lettuce contamination from hydroponic cultivation and the need for proper cleaning of these foods before consumption.

scholarly journals Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered in abattoirs in Pernambuco, Brazil

2014 ◽  
Vol 34 (4) ◽  
pp. 329-331 ◽  
Author(s):  
Mauro J.G. Bezerra ◽  
Jefferson A.L.O. Cruz ◽  
Eugênio S. Kung ◽  
José G. Silva ◽  
André S. Santos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Genomic Dna ◽  
Reproductive Organs ◽  
Infected Sheep ◽  
Fetal Membranes ◽  
Immunofluorescence Technique ◽  
Chain Reaction ◽  
Indirect Immunofluorescence Technique ◽  
Polymerase Chain

The aim of the present study was to assess the occurrence of antibodies to Toxoplasma gondii and to detect genomic DNA of the parasite in the reproductive organs, fetuses and fetal membranes of sheep in slaughterhouses in the state of Pernambuco, Brazil. The Indirect Immunofluorescence technique (IFA) was used for screening. The Polymerase Chain Reaction (PCR) was used to detect DNA of T. gondii in the animals that were positive in the serology. In the serology, 13/50 samples were positive and genomic DNA of T. gondii was detected in one uterus, tube, ovary, placenta and fetus (heart, brain and umbilical cord) sample from a sheep that was positive in the serology. The present study provides evidence of the occurrence of T. gondii DNA in the organs of the reproductive system, placenta and fetus of a naturally infected sheep.

Infrequent detection of Toxoplasma gondii genome in toxoplasmic lymphadenitis: A polymerase chain reaction study

1992 ◽  
Vol 23 (2) ◽  
pp. 154-158 ◽  
Author(s):  
Lawrence M. Weiss ◽  
Yuan-Yuan Chen ◽  
Gerald J. Berry ◽  
John G. Strickler ◽  
Ronald F. Dorfman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Chain Reaction ◽  
Reaction Study ◽  
Polymerase Chain
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