scholarly journals Comparison of Skin Scraping and Standard Superficial Skin Biopsy in the Laboratory Diagnosis of Scabies

2020 ◽  
Vol 44 (3) ◽  
pp. 164-167
Author(s):  
Orçun Zorbozan ◽  
Bengü Gerçeker Türk ◽  
Ayda Acar ◽  
Göktürk Oraloğlu ◽  
Ayşegül Ünver ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Laboratory Diagnosis ◽  
Skin Scraping ◽  
Superficial Skin

Morphological diagnosis and successful treatment of chorioptic mange in 2 South American coatis (Nasua nasua)

2021 ◽  
Author(s):  
Christoph J. Klinger ◽  
Teresa M.S.A. Boehm ◽  
Marco Roller ◽  
Tobias Knauf-Witzens
Keyword(s):  
Side Effects ◽  
Hair Loss ◽  
Complete Resolution ◽  
Clinical Signs ◽  
South American ◽  
Skin Scraping ◽  
Nasua Nasua ◽  
Superficial Skin ◽  
Morphological Diagnosis ◽  
Topical Applications

AbstractTwo female South American coatis (Nasua nasua) kept in a zoo in Southern Germany presented with pruritus and moderate hair loss at the dorsal trunk, thigh areas and alongside the tail. Otherwise the animals showed no clinical signs. While the clinical general examination as well as cytology of dermatologic samples showed no pathologic changes, Chorioptes sp. mites could be identified in a superficial skin scraping. Two consecutive topical applications of a selamectin/sarolaner spot-on formulation 4 weeks apart lead to a complete resolution. No side effects were observed.

scholarly journals An Update of Pitted Keratolysis: A Review

2018 ◽  
Vol 4 (1) ◽  
pp. 27-30
Author(s):  
Tania Hoque ◽  
Bhuiya Mohammad Mahatab Uddin
Keyword(s):  
Skin Biopsy ◽  
Bacterial Infection ◽  
Fungal Infection ◽  
Medical Research ◽  
Oral Medication ◽  
Skin Scraping ◽  
Histopathological Feature ◽  
Present Information ◽  
Hygienic Measures ◽  
Soles Of The Feet

Pitted keratolysis is a bacterial infection of the soles of the feet or less commonly, the palms of the hands. Pitted keratolysis is easily identified by its shallow, crater-like pits. Collection of specimen using swab may be helpful to identify causative bacteria and skin scraping is often taken to exclude fungal infection. The diagnosis is sometimes made by skin biopsy revealing characteristic histopathological feature of Pitted Keratolysis. Treatment generally consists of hygienic measures, sometimes supplemented by medication and perhaps on oral medication. This review is aimed to consolidate present information about aetiopathogenesis, diagnosis and management of Pitted Keratolysis. It is worth mentioning that Pitted Keratolysis is non-contagious.Journal of Current and Advance Medical Research 2017;4(1):27-30

Acetate tape impression test for diagnosis of notoedric mange in cats

2016 ◽  
Vol 19 (6) ◽  
pp. 702-705 ◽  
Author(s):  
Keytyanne O Sampaio ◽  
Lorena MB de Oliveira ◽  
Priscylla M Burmann ◽  
Reginaldo P Sousa Filho ◽  
Janaina SAM Evangelista ◽  
...  
Keyword(s):  
Good Method ◽  
Skin Scraping ◽  
Clinical Specimens ◽  
Stage Of Development ◽  
Superficial Skin ◽  
Significant Difference ◽  
Notoedres Cati ◽  
Notoedric Mange

Objectives The aim of this study was to compare the sensitivity of acetate tape impression with skin squeezing and superficial skin scraping methods for the diagnosis of burrowing mites ( Notoedres cati) in cats. Methods Samples were collected from 50 cats showing signs of notoedric mange. The most affected region was selected for sampling using acetate tape impression with skin squeezing, and superficial skin scraping. Results No significant difference was observed in the number of mites found by both methods, regardless of the stage of development of the mite. The tests showed a high correlation to the total number of mites (r = 0.928). However, in two animals it was possible to confirm the presence of N cati only by the acetate tape impression test. Conclusions and relevance We conclude that the acetate tape impression test is a good method for confirming the presence of N cati in cats. In our experience, this technique is as sensitive as superficial skin scraping, even in animals presenting with a low infestation. Moreover, the technique is less traumatic and allows the collection of clinical specimens in more sensitive locations, such as the eyelids, lips and paws.

scholarly journals Study of the Etiological Causes of Toe Web Space Lesions in Cairo, Egypt

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Hussein Mohamed Hassab-El-Naby ◽  
Yasser Fathy Mohamed ◽  
Hamed Mohamed Abdo ◽  
Mohamed Ismail Kamel ◽  
Wael Refaat Hablas ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Fungal Infection ◽  
Potassium Hydroxide ◽  
Bacterial Isolation ◽  
Skin Scraping ◽  
Detailed History ◽  
Egyptian Patients ◽  
Web Space ◽  
Skin Conditions ◽  
Light Fluorescence

Background. The etiology of foot intertrigo is varied. Several pathogens and skin conditions might play a role in toe web space lesions.Objective. To identify the possible etiological causes of toe web space lesions.Methods. 100 Egyptian patients were enrolled in this study (72 females and 28 males). Their ages ranged from 18 to 79 years. For every patient, detailed history taking, general and skin examinations, and investigations including Wood’s light examination, skin scraping for potassium hydroxide test, skin swabs for bacterial isolation, and skin biopsy all were done.Results. Among the 100 patients, positive Wood’s light fluorescence was observed in 24 and positive bacterial growth was observed in 85. With skin biopsy, 52 patients showed features characteristic for eczema, 25 showed features characteristic for fungus, 19 showed features characteristic for callosity, and 3 showed features characteristic for wart while in only 1 patient the features were characteristic for lichen planus.Conclusion. Toe web space lesions are caused by different etiological factors. The most common was interdigital eczema (52%) followed by fungal infection (25%). We suggest that patients who do not respond to antifungals should be reexamined for another primary or secondary dermatologic condition that may resemble interdigital fungal infection.

Comparison of parasitic mite retrieval methods in a population of community cats

2016 ◽  
Vol 19 (6) ◽  
pp. 657-664 ◽  
Author(s):  
Catherine Milley ◽  
Michael Dryden ◽  
Wayne Rosenkrantz ◽  
Joya Griffin ◽  
Christopher Reeder
Keyword(s):  
Room Temperature ◽  
Incidence Rates ◽  
River Valley ◽  
Ohio River ◽  
Ohio River Valley ◽  
Skin Scraping ◽  
Superficial Skin ◽  
The Usa ◽  
Parasitic Mites ◽  
Better Than

Objectives This study compared methods of mite retrieval from community cats in the Ohio River Valley region of the USA and determined incidence of parasitic mites in this region. Methods In total, 493 community cats were humanely trapped and anesthetized for a trap–neuter–return program. Cats received a dermatologic examination, ear swabs, superficial skin scraping, flea combing, acetate tape preparation and feces collection. All samples were examined microscopically. Large volumes of hair and scale from flea combing were dissolved in 10% potassium hydroxide and centrifuged with Sheather’s solution. Fecal samples were mixed with Sheather’s solution, filtered and centrifuged. Results Ear swabs were significantly ( P <0.05) better than other methods for finding chigger mites and Otodectes cynotis, and skin scraping was significantly better than ear swabs for finding Cheyletiella species. Only cats with O cynotis had clinical lesions. Mites remained identifiable for 6 months at room temperature. Mite incidence rates were as follows: Notoedres cati (1/493 cats), 0.002 (95% confidence interval [CI] 0–0.006); Lynxacarus radovskyi (2/493 cats), 0.004 (95% CI 0–0.01); Demodex gatoi (5/493 cats), 0.01 (95% CI 0.001–0.019); chigger mites (10/493 cats), 0.02 (95% CI 0.008–0.033); Cheyletiella species (12/493 cats), 0.024 (95% CI 0.011–0.038); and O cynotis (124/493 cats), 0.252 (95% CI 0.213–0.29). Conclusions and relevance Ear swabs are recommended when O cynotis or chigger mites are suspected. Skin scraping is more likely to yield positive results than ear swabs, but was not significantly better than acetate tape preparations, flea combing or fecal flotation for finding Cheyletiella species. Mites can remain identifiable for prolonged periods at room temperature. With the exception of O cynotis, the incidence of feline parasitic mites in the Ohio River Valley region of the USA is low; however, D gatoi and L radovskyi were present in the area and should be considered in cats with dermatologic disease attributable to them. In this population of community cats, asymptomatic carriage of mites was common.

scholarly journals A CASE REPORT: HYPOCHROMIC MICROCYTIC ANEMIA IN GENERALIZED SARCOPTIC MANGE INFECTED DOG

2021 ◽  
Vol 10 (2) ◽  
pp. 255-266
Author(s):  
Natalia Irene Rumpaisum ◽  
Sri Kayati Widyastuti
Keyword(s):  
Case Report ◽  
Fish Oil ◽  
Microcytic Anemia ◽  
Sarcoptic Mange ◽  
Skin Scraping ◽  
Chlorpheniramine Maleate ◽  
Hypochromic Microcytic Anemia ◽  
Superficial Skin

Skabies merupakan penyakit kulit yang disebabkan oleh infeksi tungau Sarcoptes sp. Tujuan penulisan artikel ini untuk memberikan informasi mengenai dampak dari infeksi skabies pada anjing yang menyebabkan anemia mikrositik hipokromik serta pengobatan dan penanganan yang tepat diberikan kepada anjing kasus skabies. Seekor anjing kasus berjenis kelamin jantan, dan berumur empat tahun, dengan bobot badan 9,4 kg dan memiliki masalah kulit berupa eritema pada bagian telinga, hiperkeratosis pada bagian kepala, kaki depan, abdomen, hiperpigmentasi, squama, skar, krusta dan alopesia. Masalah kulit pada anjing telah berlangsung selama delapan bulan sebelum dilakukan pemeriksaan. Dari hasil pemeriksaan kerokan kulit dengan metode superficial skin scraping ditemukan adanya larva tungau Sarcoptes sp dan hasil pemeriksaan laboratorium menunjukkan anjing mengalami anemia mikrositik hipokromik, neutofilia dan limfositopenia. Berdasarkan serangkaian pemeriksaan yang telah dilakukan, hewan didiagnosis menderita infeksi skabiosis. Pengobatan dilakukan dengan pemberian ivermectin 0,2 mL dan sabun belerang/sulfur sebagai terapi kausatif. Chlorpheniramine maleate (CTM) satu tablet dan hematodin 1,8 mL sebagai terapi simptomatik, minyak ikan/fish oil satu kapsul sebagai terapi suportif memberikan hasil yang baik dengan ditandai perubahan pada area lesi yang menunjukkan kesembuhan. Pengobatan yang telah dilakukan selama 42 hari menunjukan kondisi anjing mengalami kesembuhan ditandai dengan hilangnya squama, crusta, eritema, hiperkeratosis pada daerah punggung, wajah, telinga, leher, kaki depan, kaki belakang, pelvis dan rambut anjing tumbuh kembali normal.

Bullous Scabies: Clinical, Dermoscopic, and Pathologic Characteristics of 10 Patients

2021 ◽  
Author(s):  
Feng-Zeng Li ◽  
Meng Jia ◽  
Ke-Jun Chen ◽  
Qian Ye ◽  
Sheng Fang
Keyword(s):  
Differential Diagnosis ◽  
Basement Membrane ◽  
Skin Biopsy ◽  
Poor Response ◽  
Neutrophil Infiltration ◽  
Basement Membrane Zone ◽  
Direct Immunofluorescence ◽  
Skin Scraping ◽  
Pathological Characteristics ◽  
Clinical Variant

Bullous scabies (BS) is a rare atypical clinical variant of scabies and is easily confused with bullous disorders. The diagnosis of BS is always a challenge, and physicians often misdiagnose BS patients. Patients with BS admitted from 2012 to 2020 were enrolled in this study. The clinical, dermoscopic, and pathological characteristics of the patients were analyzed retrospectively. Ten patients with BS were enrolled in this study. Seven of the 10 patients were male. The bullae were most commonly found on the thighs and arms (80% of patients). Only 30% of patients (3/10) tested positive for mites and/or eggs by the initial skin scraping, but 100% (5/5) of the patients who received dermoscopy tested positive. Among these 10 patients, only five received a skin biopsy. Subepidermal (4/5) and intraepidermal (1/5) bullae with eosinophil and neutrophil infiltration were observed in five patients. Direct immunofluorescence (DIF) indicated linear deposition of IgG in the basement membrane zone in three patients. Physicians should consider the possibility of BS in patients with blisters, pruritus, and poor response to corticosteroids. Dermoscopy should be prioritized for the differential diagnosis of BS to exclude other bullous disorders. Finally, a biopsy should be performed on each patient with bullae.

Laboratory diagnosis of thrombosis

1977 ◽  
Vol 137 (10) ◽  
pp. 1362-1364
Author(s):  
V. Gurevich
Keyword(s):  
Laboratory Diagnosis

Eosinophilic vasculitis: an inhabitual and resistant manifestation of a vasculitis

VASA ◽  
2010 ◽  
Vol 39 (4) ◽  
pp. 344-348 ◽  
Author(s):  
Jandus ◽  
Bianda ◽  
Alerci ◽  
Gallino ◽  
Marone
Keyword(s):  
Skin Biopsy ◽  
Intravenous Iron ◽  
Small Vessel Vasculitis ◽  
High Dose ◽  
Raynaud Phenomenon ◽  
The Third ◽  
Left Elbow ◽  
Right Hand ◽  
The Right ◽  
Trunk Skin

A 55-year-old woman was referred because of diffuse pruritic erythematous lesions and an ischemic process of the third finger of her right hand. She was known to have anaemia secondary to hypermenorrhea. She presented six months before admission with a cutaneous infiltration on the left cubital cavity after a paravenous leakage of intravenous iron substitution. She then reported a progressive pruritic erythematous swelling of her left arm and lower extremities and trunk. Skin biopsy of a lesion on the right leg revealed a fibrillar, small-vessel vasculitis containing many eosinophils.Two months later she reported Raynaud symptoms in both hands, with a persistent violaceous coloration of the skin and cold sensation of her third digit of the right hand. A round 1.5 cm well-delimited swelling on the medial site of the left elbow was noted. The third digit of her right hand was cold and of violet colour. Eosinophilia (19 % of total leucocytes) was present. Doppler-duplex arterial examination of the upper extremities showed an occlusion of the cubital artery down to the palmar arcade on the right arm. Selective angiography of the right subclavian and brachial arteries showed diffuse alteration of the blood flow in the cubital artery and hand, with fine collateral circulation in the carpal region. Neither secondary causes of hypereosinophilia nor a myeloproliferative process was found. Considering the skin biopsy results and having excluded other causes of eosinophilia, we assumed the diagnosis of an eosinophilic vasculitis. Treatment with tacrolimus and high dose steroids was started, the latter tapered within 12 months and then stopped, but a dramatic flare-up of the vasculitis with Raynaud phenomenon occurred. A new immunosupressive approach with steroids and methotrexate was then introduced. This case of aggressive eosinophilic vasculitis is difficult to classify into the usual forms of vasculitis and constitutes a therapeutic challenge given the resistance to current immunosuppressive regimens.

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