scholarly journals Determination of Caffeine Content in Non-Alcoholic Beverages and Energy Drinks Using Hplc-Uv Method.

2010 ◽  
Vol 9 (1) ◽  
Author(s):  
M Karau ◽  
J Kihunyu ◽  
N Kathenya ◽  
L Wangai ◽  
D Kariuki ◽  
...  
Keyword(s):  
Energy Drinks ◽  
Alcoholic Beverages ◽  
Caffeine Content

scholarly journals Determination of Caffeine and Taurine Contents in Energy Drinks by HPLC-UV

2016 ◽  
Vol 9 ◽  
pp. 66-73 ◽  
Author(s):  
Krishna Prasad Rai ◽  
Hasta Bahadur Rai ◽  
Santosh Dahal ◽  
Saroj Chaudhary ◽  
Suraj Shrestha
Keyword(s):  
Amino Acids ◽  
Pilot Study ◽  
Quantitative Determination ◽  
Physiological Responses ◽  
Alcoholic Beverage ◽  
Energy Drinks ◽  
Energy Drink ◽  
Young Generation ◽  
Caffeine Content

Energy drinks are non-alcoholic beverage intended to enhance the psycho-physiological responses in human, which is especially popular among young generation in Nepal. It is normally high caffeinated drink added with other ingredients such as carbohydrates, amino acids, B-group of vitamins etc. In this study, 10 brands of energy drink available in Nepalese markets were taken then analyzed for quantitative determination of Caffeine and Taurine by HPLC-UV method. From the result obtained, pH and TSS values of energy drinks were found in the range of 2.96-3.81 and 6.64-18.21 respectively. Likewise, the Caffeine and Taurine content in same samples were found in the range of not detected (ND) to 35.78 mg/100 ml and ND to 387.5 mg/100 ml respectively. Only the 6 samples out of 10 were confi rmed caffeine content as per claimed in label, while only 3 samples were confi rmed for Taurine content as per label claimed. Based on this pilot study, the majority of samples did not meet the label claims in term of Caffeine and Taurine, which apparently indicated the misbranding of such drinks. Since, there is no any regulation for such energy drinks in Nepal, it seems to be a great challenge for regulation of their safety and misbranding.

scholarly journals Simultaneous Determination of Caffeine and Taurine in Energy Drinks by FT-MIR Spectroscopy Coupled with Multivariate Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Marcela González-Vázquez ◽  
Ofelia Gabriela Meza-Márquez ◽  
Tzayhri Gallardo-Velázquez ◽  
Guillermo Osorio-Revilla ◽  
José Luis Velázquez Hernández ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Fourier Transform ◽  
Standard Error ◽  
Energy Drinks ◽  
Mid Infrared ◽  
Determination Coefficient ◽  
Caffeine Content ◽  
Mir Spectroscopy ◽  
Validation Stage

Energy drinks have been studied due to their damaging side effects on the health of their consumers when consumed in excess or when combined with alcohol. Our objective was to develop chemometric models, based on Fourier-transform mid-infrared (FT-MIR) spectroscopy, to quantify the taurine and caffeine content in energy drinks rapidly and simultaneously. The taurine and caffeine content in the 50 samples ranged between 0 and 69.51 mg/100 mL and 14.92 and 1126.17 mg/100 mL, respectively. The best prediction model was obtained with the partial least squares (PLS1) algorithm; for taurine, the following values were obtained: determination coefficient of calibration (Rc2) = 0.9999, standard error of calibration (SEC) = 0.15, determination coefficient of validation (Rv2) = 0.9997, and standard error of prediction (SEP) = 0.16; for caffeine, Rc2 = 0.9999, SEC = 0.26, Rv2 = 0.9999, and SEP = 0.32. The model developed with PLS1 showed certainty in predictions during the validation stage and during application to external samples. FT-MIR coupled to chemometrics is a reliable and fast technique (compared to conventional techniques) to quantify taurine and caffeine in energy drinks simultaneously.

scholarly journals Rapid High-Performance Liquide Chromatographic Method for Quantitative Determination of Caffeine in Different Soft and Energy Drinks Available in Bangladesh

2021 ◽  
Vol 9 (3) ◽  
pp. 1081-1089
Author(s):  
Juthi Mirza ◽  
Masuda Sultana ◽  
Md. Esrafil ◽  
Shamoli Akter ◽  
Md. Jahangir Alam ◽  
...  
Keyword(s):  
High Performance ◽  
Food Additives ◽  
Reference Value ◽  
Energy Drinks ◽  
Energy Drink ◽  
Hplc Method ◽  
Soft Drinks ◽  
Caffeine Content ◽  
And Control

Caffeine is one of the commonly used food additives, which has unique flavor characteristics and bitter taste and used in soft drinks as flavor enhancer. An experimental study was designed to determine the concentration of caffeine in different brands of soft drinks and energy drinks available in Bangladesh by using HPLC. For chromatographic analysis, A Luna 5 C18 (2) 100A column (250×4.6 mm) was used at 37°C temperature at the wavelength of 272nm. Chromatographic separation was determined using buffer of sodium acetate and acetic acid with acetonitrile at a ratio of 80:20 (pH=4.0; flow rate of 1.0 ml/min). The results of this study showed that caffeine content in soft drinks ranged from 19.63 to 101.73 mg/100ml and highest concentration of caffeine found in brand 3 samples while lowest concentration found in brand 2 samples. Significantly higher concentration of caffeine (p<0.05) found in six soft drinks sample when compared to BSTI and FDA reference value except brand 2 sample (p>0.05). Quantification of caffeine in different brands of energy drink sample revealed that, four brand sample contained caffeine; among them brand 3 sample showed the highest levels of caffeine 295.86 mg/100ml and lowest amount found in brand 1 sample (101.74 mg/100ml). Concentration of Caffeine in soft and energy drinks exceeded the national and international standard recommended range hence this proposed HPLC method can be used for routine determination and control of caffeine content in different drinks.

scholarly journals Quantitative determination of caffeine in different matrices

2016 ◽  
Vol 62 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Nevena Grujić-Letić ◽  
Branislava Rakić ◽  
Emilia Šefer ◽  
Maja Milanović ◽  
Maja Nikšić ◽  
...  
Keyword(s):  
Surface Water ◽  
High Performance ◽  
Energy Drinks ◽  
Pharmaceutical Products ◽  
Hplc Method ◽  
Narcotic Analgesics ◽  
Human Waste ◽  
Caffeine Content ◽  
And Control

Caffeine is odorless, bitter taste substance which can be naturally found in coffee, cocoa, tea leaves, and is intentionally added in food and pharmaceutical products. It can also be found in surface water in small concentrations where is often used as an excellent indicator of human waste. The aim of the work is determination of caffeine content in food, beverages, analgesics and surface water using solidphase extraction followed by high-performance liquid chromatography (HPLC). Caffeine content was determined in 12 commercial tea and coffee products, non-alcoholic energy drinks and food, 5 combined preparations of analgesics and the Danube samples collected from 7 representative locations. The results showed that caffeine content in food ranged 5,6-158 mg/100 g, tea samples 24,71-30,81 mg/100 ml, coffee samples 1328-3594 mg/100 g, energy drinks 9,69-30,79 mg/100 ml and in the Danube samples 15,91-306,12 ng/l. Caffeine content in combined commercial formulations of non-narcotic analgesics of all brands did meet specifications. The data suggested that the proposed HPLC method can be used for routine determination and control of caffeine content in different matrices.

scholarly journals Determination of Caffeine Content in Commercial Energy Beverages Available in Saudi Arabian Market by Gas Chromatography-Mass Spectrometric Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Mohammed Al-Bratty ◽  
Hassan A. Alhazmi ◽  
Zia ur Rehman ◽  
Sadique A. Javed ◽  
Waquar Ahsan ◽  
...  
Keyword(s):  
Adult Population ◽  
Health Effect ◽  
Energy Drinks ◽  
Energy Drink ◽  
Spectrometric Analysis ◽  
High Concentration ◽  
Caffeine Consumption ◽  
Caffeine Content ◽  
Validation Parameters

The popularity of energy beverages among young adult population is high. These drinks are claimed to boost energy and performance and contain high concentration of caffeine as one of the several ingredients. Discrepancies have been encountered by some of the previous studies between the actual quantity of caffeine present in the product and the amount mentioned on the label, making the determination of caffeine content in these drinks very important. Thus, in this study, we analyzed the caffeine concentration in most popular energy drinks available in Saudi Arabia. The energy drink samples (n = 9) were procured from retail outlets. Sample solutions were prepared in methanol and analyzed for caffeine content by GC-MS. Chromatographic parameters were optimized to achieve optimum resolution and various validation parameters were evaluated. The method was successfully applied for the quantification of caffeine in energy drinks by directly injecting the multifold diluted samples in methanol. The method was linear (r2 = 0.999) over a concentration range of 5–25 µg/mL, specific, precise (%RSD of peak area = 0.56–0.78), and accurate (%recovery = 99.3–101.2%). The amounts of caffeine determined were found in the range of 20.82–33.72 mg/100 mL (52.05–84.3 mg/pack). Results revealed that the amount of caffeine actually present in the tested drinks varied within ±10% range from the amount specified on the product labels. The amounts of caffeine detected in tested beverages were within the USFDA safe upper limit of per-day caffeine consumption, which has indicated that the consumption of one serving of energy drink is unlikely to produce any adverse health effect.

scholarly journals Determination of Caffeine in Beverages Found in Bangladeshi Market by High Performance Liquid Chromatography (HPLC)

2021 ◽  
Vol 37 (3) ◽  
pp. 663-666
Author(s):  
G. M. M. Anwarul Hasan ◽  
Anuj Kumer Das
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Energy Drinks ◽  
Soft Drinks ◽  
Caffeine Content

Caffeine, a phycostimulant is present in several foods and drinks.In the present study, beverages of different brands in Bangladeshi market were analyzed for caffeine by high-performance liquid chromatography (HPLC) using methanol-water (40:60, v/v) as mobile phase. Caffeine content ranged from 16.33 -19.33 mg/can in soft drinks and 45.66-47.33 mg/can in energy drinks respectively. These data indicated that the levels of caffeine in Bangladeshi soft drinks and energy drinks are within the ranges reported from similar products in other countries.

scholarly journals A Pilot Study Evaluating Preferences for Caffeinated Drinks Consumption Among Mures Medical Students

2018 ◽  
Vol 75 (1) ◽  
pp. 15
Author(s):  
Iustinian SIMION ◽  
Victoria RUS ◽  
Maria SALCUDEAN ◽  
Catalin Moise DOGAR ◽  
Monica TARCEA ◽  
...  
Keyword(s):  
Medical Students ◽  
Brain Injuries ◽  
Personal Data ◽  
Energy Drinks ◽  
Health Impact ◽  
Alcoholic Beverages ◽  
Lifestyle Behaviors ◽  
Food Behaviors ◽  
Alcoholic Drinks ◽  
Food And Drink

The scale of caffeinated drinks offered for young adults is large and also is the risk for abuse followed by heart or brain injuries due to high levels of caffeine, taurine, sugars etc. For this purpose, we chose to evaluate the risk behaviors in a group of Mures medical students and assess their practices and knowledge of excess caffeine drinks intake. For this study, we used a food frequency questionnaire containing 26 questions related to personal data, food behaviors, types of food and drink consumed and the frequency of consumption. The questionnaire was applied to 427 Mures medical students in 2016. On average, 2/3 of the Mures medical students associate an excess of coffee, energy drinks and other types of caffeinated beverages, especially during the exams period. From the list of preferred drinks that our students chose, in descending order, are: coffee, carbonated beverages, alcoholic beverages and energy drinks. Students often turn to alcoholic drinks, sometimes in combination with energy drinks, raising the risk for their health. 25% have only coffee in the morning, on empty stomach, skipping breakfast. More than half of them have at least 3 coffees per day, with possible health impact on long term. Our data emphasizes once more the importance of health education in schools and universities, especially for medical students and future doctors, and the need for efficient community interventions in order to prevent lifestyle behaviors at risk.

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