scholarly journals Chemical constituents of Eremomastax speciosa (Hochst.) Cufod leaves and its cytotoxic potential on NIH-3T3 cells

2021 ◽  
Vol 34 (3) ◽  
pp. 633-640
Author(s):  
M. O. Eve ◽  
T. N. Alfred ◽  
I. I. Akripo ◽  
E. E. Ubana ◽  
I. M. Choudhary
Keyword(s):  
Fatty Acid ◽  
Chemical Constituents ◽  
Acid Ethyl Ester ◽  
Fibroblast Cell ◽  
Fatty Acid Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
3T3 Cells ◽  
Nih 3T3 ◽  
Acid Esters ◽  
Nih 3T3 Cells

This study aimed at assessing the cytotoxicity of Eremomastax speciosa crude extract on NIH-3T3 fibroblast cell lines and reporting the chemical constituents in the extract. The MTT assay on NIH-3T3 cells showed a significantly lower (p < 0.05) inhibition from E. speciosa (IC50 > 30 µg/mL) compared to cyclohexamide (IC50 > 0.8 µg/mL). This result validates the non-toxicity observed with the use of E. speciosa on normal cells at low to moderate doses. Four compounds were isolated and identified from their EIMS as well as 1D and 2D NMR spectroscopic data namely hydroxyandrographolide (1), stigmasterol glucoside (2), (Z)-4-coumaric acid 4-O-β-D-apiofuranosyl-(1’’→2’)-O-β-D-glucopyranoside (3) and 5-methoxy-4,4′-di-O-methyl- secolariciresinol-9′-monoacetate (4). These compounds are isolated from this species for the first time. Thirteen volatile constituents were detected in the extract using gas chromatography mass spectrometry (GC-MS). Besides 6,10,14-trimethy-2-pentadecanone (12.63%), mostly fatty acid esters were detected in high amounts notably ethyl hexadecanoate (16.00%), ethyl-9,12,15-octadecatrienoate (11.51%) and 9,12-octadecadienoic acid ethyl ester (8.05%). This study revealed many unsaturated fatty acid esters in E. speciosa and is noteworthy that ω-3 and ω-6 fatty acid esters were predominant, hence an added nutritional value to this plant.                     KEY WORDS: Eremomastax speciosa, Secondary metabolites, NIH-3T3 cytotoxicity, NMR, GC-MS   Bull. Chem. Soc. Ethiop. 2020, 34(3), 633-640. DOI: https://dx.doi.org/10.4314/bcse.v34i3.18

scholarly journals Chemical Constituents of Rheum ribes L.

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Consolacion Y. Ragasa ◽  
Jariel Naomi B. Bacar ◽  
Maria Margarita R. Querido ◽  
Maria Carmen S. Tan ◽  
Glenn G. Oyong ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chemical Constituents ◽  
Chemical Investigation ◽  
Fatty Acid Esters ◽  
Nmr Data ◽  
Acid Esters

Chemical investigation of the dichloromethane extract of Rheum ribes has led to the isolation of β-sitosteryl-3β- glucopyranoside-6'-O-fatty acid esters (1), β-sitosterol (2), phytyl fatty acid esters (3), triacylgly c e r o l s (4) and chlorophyllide a (5). The structures of 1-5 were identified by comparison of their NMR data with literature data.

Volatile Components of Sindora glabra Leaves

2013 ◽  
Vol 448-453 ◽  
pp. 956-959
Author(s):  
Yi Ping Chen ◽  
Sha Sha Ji ◽  
Zhen Yi Liang ◽  
Jun Feng Zhang ◽  
Zhao Hua Lin
Keyword(s):  
Ethyl Acetate Extract ◽  
Acid Ethyl Ester ◽  
Fatty Acid Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Hexadecanoic Acid ◽  
Total Volatile ◽  
Benzenedicarboxylic Acid ◽  
Total Composition ◽  
Acid Esters

The ethyl acetate extract was made from the leaves of Sindora glabra by solvent extract method and silica column chromatography, and was analyzed by means of gas chromatography-mass spectrometry. The results show that thirty-four peaks were observed, in which twenty-eight compounds were identified accounting for 93.40% of the total volatile substances detected. The major constituents are Hexadecanoic acid, ethyl ester (12.77%); (1.alpha,4a.alpha,8a.alpha) -7-methyl-4-methylene-1-isopropyl -,1,2,3,4,4a,5,6,8a-octahydro naphthalene (11.95%) and 1,2-Benzenedicarboxylic acid, butyl 2-ethylhexyl ester (11.10%). The main types of compounds are fatty acid esters (44.46%) and terpenoids (25.39%). And most of the terpenoids are sesquiterpenoids which accounted for 23.44% of the total composition.

scholarly journals MicroRNA-27a-3p promotes proliferation and migration by up-regulation of perlecan in murine fibroblast cell line NIH/3T3

2021 ◽  
Author(s):  
Jin Wang ◽  
Qingyue Ma ◽  
Wenquan Pang ◽  
Shangzhi Li ◽  
Yunwen Zou ◽  
...  
Keyword(s):  
Cell Viability ◽  
Fibroblast Cell ◽  
3T3 Cells ◽  
Expression Levels ◽  
Gene Assay ◽  
And Migration ◽  
Skin Scar ◽  
Nih 3T3 ◽  
Proliferation And Migration ◽  
Nih 3T3 Cells

IntroductionSkin scar is a common cutaneous complication, the outcome of which is unpleasant. Several microRNAs (miRs) participate in the process of skin scar formation. We aimed to explore the role of miR-27a-3p in NIH/3T3 mouse fibroblasts as well as the downstream protein and signaling cascades.Material and methodsmiR-27a-3p was aberrantly expressed in NIH/3T3 cells, followed by measurements of cell viability, migration and expressions of proteins related to proliferation and migration. Perlecan expression in cells aberrantly expressing miR-27a-3p was examined by Western blot analysis. Reporter gene assay was conducted to assess the relationship between miR-27a-3p and perlecan. Then, whether miR-27a-3p affected NIH/3T3 cells through regulating perlecan was ascertained. The effects of aberrantly expressed miR-27a-3p and perlecan on expression levels of VEGF, bFGF and key kinases in the MAPK/ERK and the PI3K/AKT pathways were detected.ResultsCell viability and migration were enhanced and protein expression levels of Cyclin D1, MMP-2 and MMP-9 were up-regulated by miR-27a-3p overexpression in NIH/3T3 cells. Then, we found that perlecan was positively correlated with miR-27a-3p expression, and its knockdown abrogated the effects of miR-27a-3p overexpression on NIH/3T3 cells. Finally, we found that the expression levels of VEGF and bFGF as well as phosphorylated levels of MAPK, ERK, PI3K and AKT were increased by miR-27a-3p overexpression, and those increases were reversed by perlecan knockdown.ConclusionsmiR-27a-3p promotes proliferation and migration of NIH/3T3 cells through up-regulating perlecan expression. Meanwhile, miR-27a-3p up-regulates expression levels of VEGF and bFGF, and activates MAPK/ERK and PI3K/AKT pathways through up-regulating perlecan expression.

scholarly journals Sterols from Echinopsis oxygona (Link) zucc. ex Pfeiff

2020 ◽  
Vol 32 (9) ◽  
pp. 2135-2138
Author(s):  
RHANNEY L. GONZALES ◽  
CHIEN-CHANG SHEN ◽  
CONSOLACION Y. RAGASA
Keyword(s):  
Fatty Acid ◽  
Sea Urchin ◽  
Chemical Constituents ◽  
The Philippines ◽  
Ornamental Plant ◽  
Fatty Acid Esters ◽  
Anticancer Properties ◽  
Ergosterol Peroxide ◽  
Easter Lily ◽  
Acid Esters

Echinopsis oxygona (Link) zucc. ex Pfeiff, commonly known as easter lily cactus and sea urchin cactus, is grown as an ornamental plant in the Philippines. Chemical investigation of the dichloromethane extracts of E. oxygona has led to the isolation of ostreasterol (1), ostreasteryl fatty acid esters (2) and ergosterol peroxide (3) from the flowers and a mixture of β-sitosterol (4) and stigmasterol (5) in about 2:1 ratio from the stems. Compounds 3-5 were reported to exhibit anticancer properties. This is the first report on the chemical constituents of E. oxygona.

scholarly journals GC-MS metabolic profiling and anti-urease activity of nonpolar fractions of Calligonum Polygonoides L. (Polygonaceae) and Crateva Adansonii DC. Prodr. (Capparaceae)

2021 ◽  
Vol 18 (9) ◽  
pp. 1955-1960
Author(s):  
Irfan Pervaiz ◽  
Saeed Ahmad ◽  
Adeel Arshad ◽  
Umair Khurshid ◽  
Abdul Basit
Keyword(s):  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Fatty Acid Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Pathological Conditions ◽  
Starting Point ◽  
Urease Inhibitory ◽  
Acid Esters

Purpose: To determine the urease-inhibitory activity and chemical constituents of fractions of Calligonum polygonoides and Crateva adansonii separated by physical properties. Methods: The anti-urease activities of different fractions of the plants (methanol, n-hexane, CHCl3, nbutanol) were evaluated using a standard procedure. The chemical constituents of the extracts with the highest urease-inhibitory activity were determined by gas chromatography-mass spectrometry. Results: The n-hexane fractions of both plants had higher urease-inhibitory activity and a lower halfmaximal inhibitory concentration (IC50) than the other extracts. GC-MS evaluation revealed that nhexane fraction of C. polygonoides was rich in fatty acids (39.36 %), sterols (22.29 %), long chain alkanes (98.5 %), and a few volatiles (5.26 %), while the n-hexane fraction of C. adansonii had high levels of alkanes (35.03 %), sterols (10.46 %), fatty acid esters (46.82 %), and triterpenes (23.76 %). Conclusion: The n-hexane fractions of the plants demonstrate high urease-inhibitory activity. Thus, these plant-based anti-urease fractions can potentially serve as a starting point for the development of novel antibacterial agents with enhanced efficacy and reduced antibiotic resistance in the treatment of pathological conditions and infections associated with urease.

scholarly journals Simultaneous determination of fatty acid esters 2-chloro-1, 3-propandiol(2-mcpd), 3-chloro-1,2- propandiol(3-mcpd) and glycidol in vegetable oils by gas chromatography mass spectrometry GC-MS/MS

2019 ◽  
Vol 2 (3) ◽  
pp. 67-73
Author(s):  
Tam Le Thanh ◽  
Duy Nguyen Thanh ◽  
Kiet Ly Tuan ◽  
Tan Phu Minh ◽  
Hung Nguyen Quoc ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid ◽  
Phenylboronic Acid ◽  
Fatty Acid Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Food Contaminants ◽  
Refined Oil ◽  
Limit Of Quantitation ◽  
Repeatability And Reproducibility ◽  
Acid Esters

The food contaminants including 3-chloro-1,2-propanediol (3-MCPD) and 3-MCPD fatty acid esters have been a matter of great concern in the past few years due to their toxicity and their occurrence in numerous foods. Recently, significant amounts of the isomeric compounds 2-chloro-1,3-propanediol (2-MCPD) fatty acid esters have been detected in refined oil samples. Gas Chromatography (GC) coupled with mass spectroscopy (MS) was used for the quantification of fatty acid esters of 2-Chloro-1,3-propandiol (2-MCPD), 3-Chloro-1,2- propandiol (3-MCPD) and glycidol in oil. Internal standards including 3-MCPD-ester-d5 (rac 1,2-bis-palmitoyl-3-chloropropanediol-d5), 2-MCPD-ester-d5 (1,3-Distearoyl-2- chloropropanediol-d5) and Glycidyl sterate-d5 were added to samples. Spiked samples were, then, incubated in NaBr/H2SO4 and H2SO4/MeOH solutions. They were, eventually, extracted with ethyl acetate and n-hexane solvents in order to convert Glycidyl esters to free MCPD and glycidol in samples before using phenylboronic acid derivative. The limit of quantitation was of 0.1 ppm (mg/kg). The recoveries at the concentration of 0.1 mg/kg ranged from 80 to 120% and the repeatability and reproducibility were less than 10%.

scholarly journals Cytotoxicity of Alpinia galanga Rhizome Crude Extract on NIH-3T3 Cells

2017 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
Ferry Sandra ◽  
Janti Sudiono ◽  
Pretty Trisfilha ◽  
Deviyanti Pratiwi
Keyword(s):  
Crude Extract ◽  
Cytotoxic Effect ◽  
Fibroblast Cell ◽  
3T3 Cells ◽  
Ginger Extract ◽  
Alpinia Galanga ◽  
Diphenyltetrazolium Bromide ◽  
Nih 3T3 ◽  
Normal Standard ◽  
Nih 3T3 Cells

BACKGROUND: Alpinia galanga (A. galanga) was reported as a potential medicinal source due to its wide effect. A. galanga rhizome crude extract (ARCE) was reported to have high cytotoxic effect in cancer cells, but low in normal cells. However half maximal inhibitory concentration (IC50) of ARCE is not clearly known yet. Hence, current study was conducted to investigate the IC50 of ARCE in normal standard fibroblast cell line, NIH-3T3 cells.METHODS: Rhizomes of A. galanga were collected, peeled, dried, milled and weighed. Extraction was performed using maceration method, then filtered and evaporated. ARCE with various concentrations were applied in NIH-3T3 cells for 24 or 48 hours. Cells were documented and counted with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Five hundreds grams of simplicia were macerated with ethanol and evaporated, 1 mg/mL crude extract with total volume of 114 mL was obtained. By addition of ARCE in NIH-3T3 cell culture, number of NIH-3T3 cells were shown less when treated with higher concentration of ARCE. Cell numbers of 0, 3.125, 6.25, 12.5, 25 and 50% ARCE treatment for 24 hours are 11,531, 11,352, 10,920, 10,365, 9,471, 8,360, respectively, meanwhile for 48 hours are 13,219, 12,686, 12,278, 11,390, 10,279, 8,390, respectively.CONCLUSION: IC50 of ARCE in 24 hours treatment was 620.5 mg/mL, while in 48 hours treatment was 666.6 mg/mL. Hence, ARCE is suggested to have low cytotoxic effect in NIH-3T3 cells.KEYWORDS: Alpinia galanga, ginger, extract, cytotoxic, MTT, NIH-3T3 

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